Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000464

Physical Location & Seq

Genomic Coordinates Reverse (-)

27428696 .. 27432062

Gene Structure 3367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1229900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 729 bp

ATGCCCGAAACTGCTTTGGGACTTTTTCCGGATGTAGGTGCCTCTTATTACTTGTCCAGACTTCCTGGATTCTTTGGAGAATATGTTGGTCTTACAGGTACCAGATTGGATGGTGCTGAAATGCTTGCTTGTGGTCTTGCCACACATTTTGTTCCATCAATAAGATTGTCTTTGTTAGAAGAAGCTTTAAACAAAGTTGATTCAAGTGATTTTGCCAATATTCAAGCAATTATAGATAAATACTCGCAGAGTCCAGCTCTGAAAAAGAAAAGTGCTTATTTCCGGATGGACGTTATAGACAAATGCTTTTCTAGAAGAACTGTAGAAGAAATTATATCTGCACTTGAGGATGAGGAGGCTAACAACAGGGCAGATGATTGGTTAACTTCAACAGTTCAAGCGCTAACGAAGGCATCACCAATGAGTCTGAAGATTTCTTTGAGATCAATTAGAGAAGGAAGGCTTCAGGGAGTTGGTCAGTGCCTTGTTCGTGAATATAGAATGGTTTCGCATGTGGTGAAAGGAGAAGTCAGCAAGGACTTCAGAGAGGGTTGCAGAGCTTTATTGTTGGACAAGGACAAGAACCCAAAGTGGGAGCCTTCTAAATTGGAGCTCGTCACTGACCATATGGTCGAGCGGTACTTCTCTAAGTTGGACGATGAAGGATGGGAAGATTTAAAGCTCCCTGCAAGATCAAACTTGCCTGTAACTGCCATTGCCAAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

243

Amino Acids

27.14

Weight (kDa)

5.77

Isoelectric Point (pI)

41.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	1 - 215	6.7e-72	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0000464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca	FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica	MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica	Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis	pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis	RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata	RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora	Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii	Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa	Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha	Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana	Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	629
Acc65I	GGTACC	1 cut(s)	98
AccB1I	GGYRCC	2 cut(s)	38, 98
AccBSI	CCGCTC	1 cut(s)	637
AccIII	TCCGGA	2 cut(s)	28, 282
AciI	CCGC	1 cut(s)	637
AcuI	CTGAAG	3 cut(s)	449, 449, 526
AfaI	GTAC	2 cut(s)	100, 641
AfeI	AGCGCT	1 cut(s)	402
AfiI	CCNNNNNNNGG	2 cut(s)	35, 592
AgsI	TTSAA	4 cut(s)	204, 224, 390, 398
AjnI	CCWGG	1 cut(s)	64
AluBI	AGCT	6 cut(s)	185, 257, 560, 613, 682, 724
AluI	AGCT	6 cut(s)	185, 257, 560, 613, 682, 724
Alw21I	GWGCWC	1 cut(s)	615
Aor13HI	TCCGGA	2 cut(s)	28, 282
Aor51HI	AGCGCT	1 cut(s)	402
Asp700I	GAANNNNTTC	1 cut(s)	505
Asp718I	GGTACC	1 cut(s)	98
AspLEI	GCGC	1 cut(s)	403
AsuHPI	GGTGA	2 cut(s)	408, 529
BanI	GGYRCC	2 cut(s)	38, 98
BanII	GRGCYC	1 cut(s)	615
Bbv12I	GWGCWC	1 cut(s)	615
BccI	CCATC	4 cut(s)	104, 163, 280, 660
BciT130I	CCWGG	1 cut(s)	66
BfaI	CTAG	1 cut(s)	312
BfmI	CTRYAG	1 cut(s)	321
BfoI	RGCGCY	1 cut(s)	404
Bme1390I	CCNGG	1 cut(s)	66
BmiI	GGNNCC	3 cut(s)	40, 100, 597
BmrFI	CCNGG	1 cut(s)	66
BmsI	GCATC	1 cut(s)	422
BplI	GAGNNNNNCTC	2 cut(s)	241, 273
BpuEI	CTTGAG	1 cut(s)	365
BsaWI	WCCGGW	2 cut(s)	28, 282
BsaXI	ACNNNNNCTCC	2 cut(s)	69, 99
Bsc4I	CCNNNNNNNGG	2 cut(s)	35, 592
Bse3DI	GCAATG	1 cut(s)	714
BseAI	TCCGGA	2 cut(s)	28, 282
BseBI	CCWGG	1 cut(s)	66
BseGI	GGATG	5 cut(s)	37, 115, 291, 355, 671
BseLI	CCNNNNNNNGG	2 cut(s)	35, 592
BseMI	GCAATG	1 cut(s)	714
BseRI	GAGGAG	1 cut(s)	368
BsgI	GTGCAG	1 cut(s)	324
BshNI	GGYRCC	2 cut(s)	38, 98
BsiHKAI	GWGCWC	1 cut(s)	615
BsiSI	CCGG	2 cut(s)	29, 283
BslFI	GGGAC	1 cut(s)	33
BslI	CCNNNNNNNGG	2 cut(s)	35, 592
BsmFI	GGGAC	1 cut(s)	33
Bsp1286I	GDGCHC	1 cut(s)	615
Bsp13I	TCCGGA	2 cut(s)	28, 282
Bsp143I	GATC	2 cut(s)	443, 692
BspACI	CCGC	1 cut(s)	637
BspEI	TCCGGA	2 cut(s)	28, 282
BspLI	GGNNCC	3 cut(s)	40, 100, 597
BspT107I	GGYRCC	2 cut(s)	38, 98
BsrBI	CCGCTC	1 cut(s)	637
BsrDI	GCAATG	1 cut(s)	714
BssMI	GATC	2 cut(s)	443, 692
Bst2UI	CCWGG	1 cut(s)	66
Bst4CI	ACNGT	2 cut(s)	322, 394
BstC8I	GCNNGC	1 cut(s)	126
BstDEI	CTNAG	1 cut(s)	648
BstF5I	GGATG	5 cut(s)	37, 115, 291, 355, 671
BstH2I	RGCGCY	1 cut(s)	404
BstHHI	GCGC	1 cut(s)	403
BstKTI	GATC	2 cut(s)	446, 695
BstMBI	GATC	2 cut(s)	443, 692
BstNI	CCWGG	1 cut(s)	66
BstNSI	RCATGY	1 cut(s)	515
BstSCI	CCNGG	1 cut(s)	64
BstSFI	CTRYAG	1 cut(s)	321
BtsCI	GGATG	5 cut(s)	37, 115, 291, 355, 671
BtsIMutI	CAGTG	2 cut(s)	485, 618
Cac8I	GCNNGC	1 cut(s)	126
CfoI	GCGC	1 cut(s)	403
Csp6I	GTAC	2 cut(s)	99, 640
CspCI	CAANNNNNGTGG	2 cut(s)	130, 165
CviAII	CATG	1 cut(s)	512
CviJI	RGCY	9 cut(s)	185, 257, 359, 463, 560, 598, 613, 682, 724
CviKI_1	RGCY	9 cut(s)	185, 257, 359, 463, 560, 598, 613, 682, 724
CviQI	GTAC	2 cut(s)	99, 640
DdeI	CTNAG	1 cut(s)	648
DpnI	GATC	2 cut(s)	445, 694
DpnII	GATC	2 cut(s)	443, 692
DraI	TTTAAA	2 cut(s)	189, 678
DrdI	GACNNNNNNGTC	1 cut(s)	629
DseDI	GACNNNNNNGTC	1 cut(s)	629
Ecl136II	GAGCTC	1 cut(s)	613
Eco24I	GRGCYC	1 cut(s)	615
Eco47III	AGCGCT	1 cut(s)	402
Eco53kI	GAGCTC	1 cut(s)	613
Eco57I	CTGAAG	3 cut(s)	449, 449, 526
EcoICRI	GAGCTC	1 cut(s)	613
EcoRII	CCWGG	1 cut(s)	64
EcoT38I	GRGCYC	1 cut(s)	615
FaeI	CATG	1 cut(s)	515
FaiI	YATR	8 cut(s)	84, 233, 296, 335, 498, 513, 627, 629
FalI	AAGNNNNNCTT	4 cut(s)	154, 186, 447, 479
FaqI	GGGAC	1 cut(s)	33
FatI	CATG	1 cut(s)	511
FauNDI	CATATG	1 cut(s)	627
FokI	GGATG	5 cut(s)	44, 122, 298, 362, 678
FriOI	GRGCYC	1 cut(s)	615
FspBI	CTAG	1 cut(s)	312
GlaI	GCGC	1 cut(s)	402
HaeII	RGCGCY	1 cut(s)	404
HapII	CCGG	2 cut(s)	29, 283
HhaI	GCGC	1 cut(s)	403
Hin1II	CATG	1 cut(s)	515
Hin6I	GCGC	1 cut(s)	401
HinP1I	GCGC	1 cut(s)	401
HincII	GTYRAC	1 cut(s)	384
HindII	GTYRAC	1 cut(s)	384
HindIII	AAGCTT	2 cut(s)	183, 722
HinfI	GANTC	4 cut(s)	69, 200, 250, 424
HpaI	GTTAAC	1 cut(s)	384
HpaII	CCGG	2 cut(s)	29, 283
HphI	GGTGA	2 cut(s)	408, 529
Hpy166II	GTNNAC	1 cut(s)	384
Hpy188I	TCNGA	3 cut(s)	261, 429, 545
Hpy188III	TCNNGA	5 cut(s)	29, 57, 283, 312, 491
Hpy8I	GTNNAC	1 cut(s)	384
HpyAV	CCTTC	5 cut(s)	403, 449, 453, 609, 656
HpyCH4III	ACNGT	2 cut(s)	322, 394
HpyCH4IV	ACGT	1 cut(s)	291
HpyCH4V	TGCA	3 cut(s)	341, 555, 689
HpyF3I	CTNAG	1 cut(s)	648
HpySE526I	ACGT	1 cut(s)	291
Hsp92II	CATG	1 cut(s)	515
HspAI	GCGC	1 cut(s)	401
Kpn2I	TCCGGA	2 cut(s)	28, 282
KpnI	GGTACC	1 cut(s)	102
KspAI	GTTAAC	1 cut(s)	384
Kzo9I	GATC	2 cut(s)	443, 692
LmnI	GCTCC	3 cut(s)	595, 610, 687
LweI	GCATC	1 cut(s)	422
MaeI	CTAG	1 cut(s)	312
MaeII	ACGT	1 cut(s)	291
MaeIII	GTNAC	2 cut(s)	616, 706
MalI	GATC	2 cut(s)	445, 694
MbiI	CCGCTC	1 cut(s)	637
MboI	GATC	2 cut(s)	443, 692
MboII	GAAGA	5 cut(s)	191, 327, 338, 442, 683
MhlI	GDGCHC	1 cut(s)	615
MluCI	AATT	4 cut(s)	228, 330, 447, 605
MlyI	GAGTC	2 cut(s)	259, 433
MmeI	TCCRAC	2 cut(s)	549, 633
MnlI	CCTC	5 cut(s)	52, 340, 346, 349, 541
MroI	TCCGGA	2 cut(s)	28, 282
MroXI	GAANNNNTTC	1 cut(s)	505
MseI	TTAA	4 cut(s)	188, 383, 677, 727
MspI	CCGG	2 cut(s)	29, 283
MspR9I	CCNGG	1 cut(s)	66
MvaI	CCWGG	1 cut(s)	66
NdeI	CATATG	1 cut(s)	627
NdeII	GATC	2 cut(s)	443, 692
NlaIII	CATG	1 cut(s)	515
NlaIV	GGNNCC	3 cut(s)	40, 100, 597
NmuCI	GTSAC	1 cut(s)	616
NspI	RCATGY	1 cut(s)	515
PdmI	GAANNNNTTC	1 cut(s)	505
PfeI	GAWTC	2 cut(s)	69, 200
PfoI	TCCNGGA	1 cut(s)	64
PleI	GAGTC	2 cut(s)	258, 432
PpsI	GAGTC	2 cut(s)	258, 432
Psp124BI	GAGCTC	1 cut(s)	615
Psp6I	CCWGG	1 cut(s)	64
PspGI	CCWGG	1 cut(s)	64
PspN4I	GGNNCC	3 cut(s)	40, 100, 597
RsaI	GTAC	2 cut(s)	100, 641
RsaNI	GTAC	2 cut(s)	99, 640
SacI	GAGCTC	1 cut(s)	615
SaqAI	TTAA	4 cut(s)	188, 383, 677, 727
Sau3AI	GATC	2 cut(s)	443, 692
SchI	GAGTC	2 cut(s)	259, 433
ScrFI	CCNGG	1 cut(s)	66
SduI	GDGCHC	1 cut(s)	615
SetI	ASST	9 cut(s)	40, 100, 187, 259, 294, 562, 615, 684, 726
SfaNI	GCATC	1 cut(s)	422
SfcI	CTRYAG	1 cut(s)	321
SmlI	CTYRAG	1 cut(s)	344
SmoI	CTYRAG	1 cut(s)	344
Sse9I	AATT	4 cut(s)	228, 330, 447, 605
SsiI	CCGC	1 cut(s)	637
SspI	AATATT	1 cut(s)	220
SspMI	CTAG	1 cut(s)	312
SstI	GAGCTC	1 cut(s)	615
StyD4I	CCNGG	1 cut(s)	64
TaaI	ACNGT	2 cut(s)	322, 394
TaiI	ACGT	1 cut(s)	294
TaqI	TCGA	1 cut(s)	633
TasI	AATT	4 cut(s)	228, 330, 447, 605
TfiI	GAWTC	2 cut(s)	69, 200
Tru1I	TTAA	4 cut(s)	188, 383, 677, 727
Tru9I	TTAA	4 cut(s)	188, 383, 677, 727
TscAI	CASTG	2 cut(s)	485, 625
TseFI	GTSAC	1 cut(s)	616
Tsp45I	GTSAC	1 cut(s)	616
TspDTI	ATGAA	1 cut(s)	675
TspRI	CASTG	2 cut(s)	485, 625
XbaI	TCTAGA	1 cut(s)	311
XceI	RCATGY	1 cut(s)	515
XmnI	GAANNNNTTC	1 cut(s)	505
XspI	CTAG	1 cut(s)	312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.