Prupe.2G207200_v2.0.a1

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000464
Physical Location & Seq
Forward (+)
24108488 .. 24110268
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G207200.1

Sequence Viewer

Length: 1176 bp
ATGGCTTCTTTCCAACTTGATGACCAGGTTCTAGTACAAGAGACTATGTTCGTGAGAACATTGACCTTGAACAGGCCTCGCCAACTGAACACCCTTTCCCTTGAAATGATCTCTCAGCTGTCAGAACTTTTCCTGGCCTATGAGGACGATGCCAATGCCAAGTTGATCATTCTCAAGGGTAGAGGAAGAGCATTTTGTGCTGGCGGCAATCTAGCCGCCGTGCATCGTCACTTTCTTGAAGGAAATCTGAAATATGGTGAAAAAGTTAGTCAGAAGATCATCAACTTAACCTATTTGATAGCAACATACAGTAAACCCCAGGTTTCAATTCTGAATGGGATTGCCATGGGAGCTGGGGTTGGTATTTCAGTACACGGAAAATTCCGTGTAGCAACAGAGAATTCTATCTTTGCAACGCCAGAAAATGGTCTGGGATTTTTCCCTAATGTGGGTGCTTCTTATTTCTTGTCAAGACTCCCTGGATTTTTTGGAGAGTATTTGGGTCTCACAGGTGCCAGGTTGGATGGGGCTGAAATGCTTGCTTGTGGTCTAGCAACTCACTTTGTTCCCTCAGCCAAATTGCCTCTACTGGAAAAGGCTCTGATTTCCAGAGCTGCTTCAGTTACTTCCTCAAGCTTTGATCTTGCTTTTATTTCAGCAATTATAGACGAACACTCCCTACGGCAACCGGCTCTGAACGAGAAAAGTGCTCTTCACAAAATGGATGTCATTGACAAGTGCTTTTCCAGACCAACAGTTGAAGAAATCTTAAGTGCCCTTGAGAGGGAGGCTGCAGAAGCACACATGGCAGATGATGACCATCGTCATGAATGGTTAGCTTTGACGATTCTTTTGCTGAAGAAGGCGTCGCCGATGAGCTTGAAGATTTGTTTGAGATCCATTAGAGAAGGAAGGGTGCAGGCAATTGACGAATGCCTTGTCCGCGAGTACAGAATAACTAGCCATATACTGCAAGGGCAAATAAGCAAGGACTTCAGAGAAGGTTGCAGAGCTATCGTGTGGGACAAGGATAAGAAACCAAAGTGGAAGCCTTCTAGTTTGGAACTCATCACTGACCATATGGTTGACCACTACTTCTCTAAGTTGGATGGTGATGAAGAATTAAAGCTCCCTCAAAGATCCAACTTGGCTGCATTTGCCAATGCCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

392

Amino Acids

43.43

Weight (kDa)

7.19

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 425
AccII CGCG 1 cut(s) 945
AciI CCGC 3 cut(s) 204, 216, 943
AclWI GGATC 2 cut(s) 891, 1134
AcsI RAATTY 2 cut(s) 380, 400
AcuI CTGAAG 3 cut(s) 603, 878, 979
AcyI GRCGYC 1 cut(s) 866
AfaI GTAC 3 cut(s) 36, 372, 950
AfiI CCNNNNNNNGG 6 cut(s) 72, 425, 448, 449, 783, 784
AflII CTTAAG 1 cut(s) 769
AgsI TTSAA 6 cut(s) 70, 104, 239, 327, 761, 883
AjnI CCWGG 5 cut(s) 24, 132, 318, 478, 515
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 9 cut(s) 118, 353, 614, 636, 839, 879, 1013, 1129, 1171
AluI AGCT 9 cut(s) 118, 353, 614, 636, 839, 879, 1013, 1129, 1171
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 35, 509
AlwI GGATC 2 cut(s) 891, 1134
AoxI GGCC 2 cut(s) 74, 135
ApeKI GCWGC 3 cut(s) 614, 791, 1151
ApoI RAATTY 2 cut(s) 380, 400
ArsI GACNNNNNNTTYG 6 cut(s) 711, 743, 835, 867, 924, 956
AsuHPI GGTGA 2 cut(s) 269, 1124
BaeGI GKGCMC 1 cut(s) 778
BanI GGYRCC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 712
BbvCI CCTCAGC 1 cut(s) 571
BbvI GCAGC 3 cut(s) 601, 778, 1138
BccI CCATC 3 cut(s) 518, 828, 1103
BceAI ACGGC 2 cut(s) 203, 698
BcgI CGANNNNNNTGC 8 cut(s) 137, 171, 689, 723, 835, 869, 997, 1031
BciT130I CCWGG 5 cut(s) 26, 134, 320, 480, 517
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 35, 509
BfaI CTAG 5 cut(s) 32, 212, 551, 960, 1056
BfmI CTRYAG 1 cut(s) 792
BfrI CTTAAG 1 cut(s) 769
BisI GCNGC 5 cut(s) 205, 216, 615, 792, 1152
BlsI GCNGC 5 cut(s) 206, 217, 616, 793, 1153
Bme1390I CCNGG 5 cut(s) 26, 134, 320, 480, 517
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 5 cut(s) 26, 134, 320, 480, 517
BmsI GCATC 2 cut(s) 139, 232
BoxI GACNNNNGTC 1 cut(s) 822
Bpu10I CCTNAGC 1 cut(s) 571
BpuEI CTTGAG 3 cut(s) 158, 616, 800
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 509
BsaJI CCNNGG 3 cut(s) 318, 345, 478
BsaXI ACNNNNNCTCC 4 cut(s) 342, 372, 659, 689
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 425, 448, 449, 783, 784
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 5 cut(s) 26, 134, 320, 480, 517
BseDI CCNNGG 3 cut(s) 318, 345, 478
BseGI GGATG 3 cut(s) 529, 730, 1114
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 6 cut(s) 72, 425, 448, 449, 783, 784
BseMII CTCAG 2 cut(s) 128, 585
BseNI ACTGG 1 cut(s) 594
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 3 cut(s) 601, 778, 1138
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 938
Bsh1236I CGCG 1 cut(s) 945
BshFI GGCC 2 cut(s) 76, 137
BshNI GGYRCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 1 cut(s) 689
BslFI GGGAC 1 cut(s) 1037
BslI CCNNNNNNNGG 6 cut(s) 72, 425, 448, 449, 783, 784
BsmAI GTCTC 2 cut(s) 35, 509
BsmFI GGGAC 1 cut(s) 1037
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 2 cut(s) 76, 137
Bso31I GGTCTC 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 712, 778
Bsp143I GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 3 cut(s) 204, 216, 943
BspANI GGCC 2 cut(s) 76, 137
BspCNI CTCAG 2 cut(s) 127, 584
BspFNI CGCG 1 cut(s) 945
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 796
BspPI GGATC 2 cut(s) 891, 1134
BspQI GCTCTTC 2 cut(s) 181, 717
BspT107I GGYRCC 1 cut(s) 512
BspTI CTTAAG 1 cut(s) 769
BspTNI GGTCTC 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 318, 345, 478
BssMI GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
BssNI GRCGYC 1 cut(s) 866
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 5 cut(s) 26, 134, 320, 480, 517
Bst4CI ACNGT 2 cut(s) 311, 757
Bst6I CTCTTC 2 cut(s) 181, 717
BstACI GRCGYC 1 cut(s) 866
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 202, 540, 921
BstDEI CTNAG 3 cut(s) 114, 571, 1101
BstDSI CCRYGG 1 cut(s) 345
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 3 cut(s) 529, 730, 1114
BstFNI CGCG 1 cut(s) 945
BstKTI GATC 6 cut(s) 111, 168, 279, 643, 899, 1142
BstMAI GTCTC 2 cut(s) 35, 509
BstMBI GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
BstMWI GCNNNNNNNGC 6 cut(s) 197, 350, 797, 806, 942, 1157
BstNI CCWGG 5 cut(s) 26, 134, 320, 480, 517
BstPAI GACNNNNGTC 1 cut(s) 822
BstSCI CCNGG 5 cut(s) 24, 132, 318, 478, 515
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 778
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 3 cut(s) 601, 778, 1138
BstX2I RGATCY 2 cut(s) 896, 1139
BstYI RGATCY 2 cut(s) 896, 1139
BsuRI GGCC 2 cut(s) 76, 137
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 3 cut(s) 529, 730, 1114
BtsIMutI CAGTG 1 cut(s) 1071
Cac8I GCNNGC 3 cut(s) 202, 540, 921
CciI TCATGA 1 cut(s) 826
Cfr10I RCCGGY 1 cut(s) 688
CseI GACGC 1 cut(s) 855
CsiI ACCWGGT 1 cut(s) 24
Csp6I GTAC 3 cut(s) 35, 371, 949
CviAII CATG 3 cut(s) 346, 805, 827
CviQI GTAC 3 cut(s) 35, 371, 949
DdeI CTNAG 3 cut(s) 114, 571, 1101
DpnI GATC 6 cut(s) 110, 167, 278, 642, 898, 1141
DpnII GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
Eam1104I CTCTTC 2 cut(s) 181, 717
EarI CTCTTC 2 cut(s) 181, 717
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 76
Eco31I GGTCTC 1 cut(s) 509
Eco57I CTGAAG 3 cut(s) 603, 878, 979
EcoNI CCTNNNNNAGG 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 400
EcoRII CCWGG 5 cut(s) 24, 132, 318, 478, 515
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 349, 808, 830
FaqI GGGAC 1 cut(s) 1037
FatI CATG 3 cut(s) 345, 804, 826
FauNDI CATATG 1 cut(s) 1080
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 205, 216, 615, 792, 1152
FokI GGATG 3 cut(s) 536, 737, 1121
Fsp4HI GCNGC 5 cut(s) 205, 216, 615, 792, 1152
FspBI CTAG 5 cut(s) 32, 212, 551, 960, 1056
GluI GCNGC 5 cut(s) 205, 216, 615, 792, 1152
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 2 cut(s) 76, 137
HapII CCGG 1 cut(s) 689
HgaI GACGC 1 cut(s) 855
Hin1I GRCGYC 1 cut(s) 866
Hin1II CATG 3 cut(s) 349, 808, 830
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 2 cut(s) 634, 1169
HinfI GANTC 2 cut(s) 474, 847
HpaII CCGG 1 cut(s) 689
HphI GGTGA 2 cut(s) 269, 1124
Hpy166II GTNNAC 3 cut(s) 314, 373, 1087
Hpy188I TCNGA 7 cut(s) 124, 249, 273, 333, 603, 696, 998
Hpy188III TCNNGA 6 cut(s) 52, 236, 471, 609, 747, 827
Hpy8I GTNNAC 3 cut(s) 314, 373, 1087
Hpy99I CGWCG 1 cut(s) 871
HpyAV CCTTC 6 cut(s) 233, 856, 902, 906, 995, 1062
HpyCH4III ACNGT 2 cut(s) 311, 757
HpyCH4V TGCA 7 cut(s) 223, 413, 794, 919, 973, 1008, 1154
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 350, 797, 806, 942, 1157
HpyF3I CTNAG 3 cut(s) 114, 571, 1101
Hsp92I GRCGYC 1 cut(s) 866
Hsp92II CATG 3 cut(s) 349, 808, 830
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
LguI GCTCTTC 2 cut(s) 181, 717
LmnI GCTCC 2 cut(s) 350, 1134
Lsp1109I GCAGC 3 cut(s) 601, 778, 1138
LweI GCATC 2 cut(s) 139, 232
MabI ACCWGGT 1 cut(s) 24
MaeI CTAG 5 cut(s) 32, 212, 551, 960, 1056
MaeIII GTNAC 2 cut(s) 227, 622
MalI GATC 6 cut(s) 110, 167, 278, 642, 898, 1141
MboI GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
MboII GAAGA 7 cut(s) 198, 286, 704, 773, 871, 895, 1130
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 2 cut(s) 896, 1139
MhlI GDGCHC 2 cut(s) 712, 778
MluCI AATT 7 cut(s) 327, 380, 400, 578, 660, 924, 1121
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 4 cut(s) 37, 501, 1086, 1167
MnlI CCTC 9 cut(s) 87, 136, 176, 580, 594, 640, 777, 781, 1143
MseI TTAA 3 cut(s) 287, 770, 1124
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 1 cut(s) 689
MspR9I CCNGG 5 cut(s) 26, 134, 320, 480, 517
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 5 cut(s) 26, 134, 320, 480, 517
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 6 cut(s) 197, 350, 797, 806, 942, 1157
NcoI CCATGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 1080
NdeII GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
NlaIII CATG 3 cut(s) 349, 808, 830
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 227
PagI TCATGA 1 cut(s) 826
PceI AGGCCT 1 cut(s) 76
PciSI GCTCTTC 2 cut(s) 181, 717
PctI GAATGC 1 cut(s) 938
PfeI GAWTC 1 cut(s) 847
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 5 cut(s) 206, 217, 616, 793, 1153
PleI GAGTC 1 cut(s) 468
PpsI GAGTC 1 cut(s) 468
PshAI GACNNNNGTC 1 cut(s) 822
Psp6I CCWGG 5 cut(s) 24, 132, 318, 478, 515
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 5 cut(s) 24, 132, 318, 478, 515
PspN4I GGNNCC 1 cut(s) 514
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 2 cut(s) 896, 1139
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 3 cut(s) 36, 372, 950
RsaNI GTAC 3 cut(s) 35, 371, 949
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 2 cut(s) 181, 717
SaqAI TTAA 3 cut(s) 287, 770, 1124
SatI GCNGC 5 cut(s) 205, 216, 615, 792, 1152
Sau3AI GATC 6 cut(s) 108, 165, 276, 640, 896, 1139
SchI GAGTC 1 cut(s) 468
ScrFI CCNGG 5 cut(s) 26, 134, 320, 480, 517
SduI GDGCHC 2 cut(s) 712, 778
SexAI ACCWGGT 1 cut(s) 24
SfaNI GCATC 2 cut(s) 139, 232
SfcI CTRYAG 1 cut(s) 792
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 4 cut(s) 173, 631, 769, 779
SmoI CTYRAG 4 cut(s) 173, 631, 769, 779
Sse9I AATT 7 cut(s) 327, 380, 400, 578, 660, 924, 1121
SseBI AGGCCT 1 cut(s) 76
SsiI CCGC 3 cut(s) 204, 216, 943
SspMI CTAG 5 cut(s) 32, 212, 551, 960, 1056
StuI AGGCCT 1 cut(s) 76
StyD4I CCNGG 5 cut(s) 24, 132, 318, 478, 515
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 311, 757
TasI AATT 7 cut(s) 327, 380, 400, 578, 660, 924, 1121
TatI WGTACW 3 cut(s) 34, 370, 948
TauI GCSGC 2 cut(s) 207, 218
TfiI GAWTC 1 cut(s) 847
Tru1I TTAA 3 cut(s) 287, 770, 1124
Tru9I TTAA 3 cut(s) 287, 770, 1124
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 3 cut(s) 614, 791, 1151
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 843, 1131
TspGWI ACGGA 2 cut(s) 374, 390
TspRI CASTG 1 cut(s) 1078
Van91I CCANNNNNTGG 1 cut(s) 425
Vha464I CTTAAG 1 cut(s) 769
XagI CCTNNNNNAGG 1 cut(s) 70
XapI RAATTY 2 cut(s) 380, 400
XspI CTAG 5 cut(s) 32, 212, 551, 960, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.