Belongs to the enoyl-CoA hydratase isomerase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| ECH_2 | PF16113 | 23 - 221 | 4.1e-45 | Enoyl-CoA hydratase/isomerase |
| KH_1 | PF00013 | 273 - 325 | 1.1e-07 | KH domain |
| Beta-prop_FBX42 | PF13415 | 324 - 381 | 7.6e-06 | FBX42, beta-propeller domain |
| Kelch_KLHDC2_KLHL20_DRC7 | PF24681 | 334 - 384 | 3.1e-08 | KLHDC2/KLHL20/DRC7 Kelch-repeats domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1271 |
| AatII | GACGTC | 1 cut(s) | 1215 |
| AccB1I | GGYRCC | 2 cut(s) | 74, 935 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 289 |
| AccI | GTMKAC | 1 cut(s) | 894 |
| AccII | CGCG | 1 cut(s) | 10 |
| AciI | CCGC | 7 cut(s) | 10, 738, 747, 753, 1015, 1296, 1404 |
| AclWI | GGATC | 2 cut(s) | 1406, 1414 |
| AcsI | RAATTY | 1 cut(s) | 1305 |
| AcuI | CTGAAG | 1 cut(s) | 1167 |
| AcyI | GRCGYC | 2 cut(s) | 801, 1212 |
| AdeI | CACNNNGTG | 1 cut(s) | 1291 |
| AfaI | GTAC | 3 cut(s) | 824, 1258, 1523 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 168, 289, 352, 775 |
| AgeI | ACCGGT | 1 cut(s) | 819 |
| AgsI | TTSAA | 4 cut(s) | 189, 413, 445, 709 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 901 |
| AjnI | CCWGG | 3 cut(s) | 162, 1392, 1438 |
| Alw21I | GWGCWC | 2 cut(s) | 633, 657 |
| Alw26I | GTCTC | 3 cut(s) | 10, 971, 1356 |
| AlwI | GGATC | 2 cut(s) | 1406, 1414 |
| Ama87I | CYCGRG | 1 cut(s) | 1047 |
| AoxI | GGCC | 1 cut(s) | 1349 |
| ApeKI | GCWGC | 4 cut(s) | 362, 478, 481, 1510 |
| ApoI | RAATTY | 1 cut(s) | 1305 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1369, 1401 |
| AsiGI | ACCGGT | 1 cut(s) | 819 |
| AspLEI | GCGC | 1 cut(s) | 182 |
| AspS9I | GGNCC | 3 cut(s) | 640, 1153, 1349 |
| AsuC2I | CCSGG | 1 cut(s) | 771 |
| AsuHPI | GGTGA | 7 cut(s) | 377, 423, 506, 554, 1136, 1176, 1382 |
| AsuII | TTCGAA | 1 cut(s) | 913 |
| AsuNHI | GCTAGC | 1 cut(s) | 142 |
| AvaI | CYCGRG | 1 cut(s) | 1047 |
| AvaII | GGWCC | 2 cut(s) | 640, 1153 |
| BaeGI | GKGCMC | 1 cut(s) | 833 |
| BanI | GGYRCC | 2 cut(s) | 74, 935 |
| BanII | GRGCYC | 1 cut(s) | 633 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 263, 295 |
| Bbv12I | GWGCWC | 2 cut(s) | 633, 657 |
| BbvI | GCAGC | 4 cut(s) | 349, 468, 490, 1497 |
| BccI | CCATC | 4 cut(s) | 80, 640, 1100, 1213 |
| BceAI | ACGGC | 1 cut(s) | 751 |
| BciT130I | CCWGG | 3 cut(s) | 164, 1394, 1440 |
| BclI | TGATCA | 1 cut(s) | 382 |
| BcnI | CCSGG | 1 cut(s) | 771 |
| BcoDI | GTCTC | 3 cut(s) | 10, 971, 1356 |
| BfaI | CTAG | 6 cut(s) | 113, 143, 152, 176, 236, 666 |
| BfmI | CTRYAG | 1 cut(s) | 347 |
| BfoI | RGCGCY | 1 cut(s) | 183 |
| BisI | GCNGC | 5 cut(s) | 363, 479, 482, 754, 1511 |
| BlsI | GCNGC | 5 cut(s) | 364, 480, 483, 755, 1512 |
| Bme1390I | CCNGG | 4 cut(s) | 164, 771, 1394, 1440 |
| Bme18I | GGWCC | 2 cut(s) | 640, 1153 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 901 |
| BmeT110I | CYCGRG | 1 cut(s) | 1047 |
| BmgT120I | GGNCC | 3 cut(s) | 640, 1153, 1349 |
| BmiI | GGNNCC | 4 cut(s) | 76, 937, 1143, 1155 |
| BmrFI | CCNGG | 4 cut(s) | 164, 771, 1394, 1440 |
| BmrI | ACTGGG | 1 cut(s) | 827 |
| BmsI | GCATC | 1 cut(s) | 437 |
| BmtI | GCTAGC | 1 cut(s) | 146 |
| BmuI | ACTGGG | 1 cut(s) | 827 |
| BpmI | CTGGAG | 2 cut(s) | 1320, 1415 |
| Bpu14I | TTCGAA | 1 cut(s) | 913 |
| BpuEI | CTTGAG | 1 cut(s) | 1232 |
| BpuMI | CCSGG | 1 cut(s) | 771 |
| BsaBI | GATNNNNATC | 1 cut(s) | 914 |
| BsaHI | GRCGYC | 2 cut(s) | 801, 1212 |
| BsaI | GGTCTC | 2 cut(s) | 10, 971 |
| BsaJI | CCNNGG | 3 cut(s) | 162, 769, 804 |
| BsaWI | WCCGGW | 1 cut(s) | 819 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 861, 891 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 168, 289, 352, 775 |
| Bse118I | RCCGGY | 1 cut(s) | 819 |
| Bse1I | ACTGG | 2 cut(s) | 833, 1030 |
| Bse3DI | GCAATG | 1 cut(s) | 1036 |
| Bse8I | GATNNNNATC | 1 cut(s) | 914 |
| BseBI | CCWGG | 3 cut(s) | 164, 1394, 1440 |
| BseDI | CCNNGG | 3 cut(s) | 162, 769, 804 |
| BseGI | GGATG | 3 cut(s) | 91, 298, 1501 |
| BseJI | GATNNNNATC | 1 cut(s) | 914 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 168, 289, 352, 775 |
| BseMI | GCAATG | 1 cut(s) | 1036 |
| BseMII | CTCAG | 2 cut(s) | 240, 1294 |
| BseNI | ACTGG | 2 cut(s) | 833, 1030 |
| BseRI | GAGGAG | 4 cut(s) | 501, 756, 879, 882 |
| BseSI | GKGCMC | 1 cut(s) | 833 |
| BseXI | GCAGC | 4 cut(s) | 349, 468, 490, 1497 |
| BseYI | CCCAGC | 1 cut(s) | 931 |
| BsgI | GTGCAG | 1 cut(s) | 987 |
| Bsh1236I | CGCG | 1 cut(s) | 10 |
| BshFI | GGCC | 1 cut(s) | 1351 |
| BshNI | GGYRCC | 2 cut(s) | 74, 935 |
| BshTI | ACCGGT | 1 cut(s) | 819 |
| BsiHKAI | GWGCWC | 2 cut(s) | 633, 657 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1047 |
| BsiSI | CCGG | 2 cut(s) | 771, 820 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 168, 289, 352, 775 |
| BsmAI | GTCTC | 3 cut(s) | 10, 971, 1356 |
| BsnI | GGCC | 1 cut(s) | 1351 |
| Bso31I | GGTCTC | 2 cut(s) | 10, 971 |
| BsoBI | CYCGRG | 1 cut(s) | 1047 |
| Bsp119I | TTCGAA | 1 cut(s) | 913 |
| Bsp1286I | GDGCHC | 3 cut(s) | 633, 657, 833 |
| Bsp143I | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| BspACI | CCGC | 7 cut(s) | 10, 738, 747, 753, 1015, 1296, 1404 |
| BspANI | GGCC | 1 cut(s) | 1351 |
| BspCNI | CTCAG | 2 cut(s) | 241, 1293 |
| BspFNI | CGCG | 1 cut(s) | 10 |
| BspHI | TCATGA | 2 cut(s) | 508, 1215 |
| BspLI | GGNNCC | 4 cut(s) | 76, 937, 1143, 1155 |
| BspOI | GCTAGC | 1 cut(s) | 146 |
| BspPI | GGATC | 2 cut(s) | 1406, 1414 |
| BspT104I | TTCGAA | 1 cut(s) | 913 |
| BspT107I | GGYRCC | 2 cut(s) | 74, 935 |
| BspTNI | GGTCTC | 2 cut(s) | 10, 971 |
| BsrDI | GCAATG | 1 cut(s) | 1036 |
| BsrFI | RCCGGY | 1 cut(s) | 819 |
| BsrI | ACTGG | 2 cut(s) | 833, 1030 |
| BssAI | RCCGGY | 1 cut(s) | 819 |
| BssECI | CCNNGG | 3 cut(s) | 162, 769, 804 |
| BssMI | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| BssNI | GRCGYC | 2 cut(s) | 801, 1212 |
| Bst2UI | CCWGG | 3 cut(s) | 164, 1394, 1440 |
| Bst4CI | ACNGT | 4 cut(s) | 325, 814, 928, 1008 |
| Bst6I | CTCTTC | 3 cut(s) | 266, 856, 1143 |
| BstACI | GRCGYC | 2 cut(s) | 801, 1212 |
| BstBI | TTCGAA | 1 cut(s) | 913 |
| BstC8I | GCNNGC | 4 cut(s) | 102, 144, 360, 1515 |
| BstDEI | CTNAG | 5 cut(s) | 138, 249, 419, 887, 1280 |
| BstEII | GGTNACC | 1 cut(s) | 1017 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 350 |
| BstF5I | GGATG | 3 cut(s) | 91, 298, 1501 |
| BstFNI | CGCG | 1 cut(s) | 10 |
| BstH2I | RGCGCY | 1 cut(s) | 183 |
| BstHHI | GCGC | 1 cut(s) | 182 |
| BstKTI | GATC | 4 cut(s) | 385, 1129, 1409, 1414 |
| BstMAI | GTCTC | 3 cut(s) | 10, 971, 1356 |
| BstMBI | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 148, 744, 753, 1107 |
| BstNI | CCWGG | 3 cut(s) | 164, 1394, 1440 |
| BstNSI | RCATGY | 1 cut(s) | 1071 |
| BstPI | GGTNACC | 1 cut(s) | 1017 |
| BstSCI | CCNGG | 4 cut(s) | 162, 769, 1392, 1438 |
| BstSFI | CTRYAG | 1 cut(s) | 347 |
| BstSLI | GKGCMC | 1 cut(s) | 833 |
| BstUI | CGCG | 1 cut(s) | 10 |
| BstV1I | GCAGC | 4 cut(s) | 349, 468, 490, 1497 |
| BstX2I | RGATCY | 1 cut(s) | 1406 |
| BstYI | RGATCY | 1 cut(s) | 1406 |
| BsuRI | GGCC | 1 cut(s) | 1351 |
| BtsCI | GGATG | 3 cut(s) | 91, 298, 1501 |
| BtsIMutI | CAGTG | 4 cut(s) | 330, 840, 1013, 1061 |
| Cac8I | GCNNGC | 4 cut(s) | 102, 144, 360, 1515 |
| CciI | TCATGA | 2 cut(s) | 508, 1215 |
| CfoI | GCGC | 1 cut(s) | 182 |
| Cfr10I | RCCGGY | 1 cut(s) | 819 |
| Cfr13I | GGNCC | 3 cut(s) | 640, 1153, 1349 |
| CseI | GACGC | 1 cut(s) | 809 |
| Csp6I | GTAC | 3 cut(s) | 823, 1257, 1522 |
| CspAI | ACCGGT | 1 cut(s) | 819 |
| CviAII | CATG | 5 cut(s) | 41, 509, 530, 1068, 1216 |
| CviQI | GTAC | 3 cut(s) | 823, 1257, 1522 |
| DdeI | CTNAG | 5 cut(s) | 138, 249, 419, 887, 1280 |
| DpnI | GATC | 4 cut(s) | 384, 1128, 1408, 1413 |
| DpnII | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| DraIII | CACNNNGTG | 1 cut(s) | 1291 |
| DriI | GACNNNNNGTC | 1 cut(s) | 901 |
| Eam1104I | CTCTTC | 3 cut(s) | 266, 856, 1143 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 901 |
| EarI | CTCTTC | 3 cut(s) | 266, 856, 1143 |
| EciI | GGCGGA | 2 cut(s) | 753, 1419 |
| Ecl136II | GAGCTC | 1 cut(s) | 631 |
| Eco24I | GRGCYC | 1 cut(s) | 633 |
| Eco31I | GGTCTC | 2 cut(s) | 10, 971 |
| Eco47I | GGWCC | 2 cut(s) | 640, 1153 |
| Eco53kI | GAGCTC | 1 cut(s) | 631 |
| Eco57I | CTGAAG | 1 cut(s) | 1167 |
| Eco88I | CYCGRG | 1 cut(s) | 1047 |
| Eco91I | GGTNACC | 1 cut(s) | 1017 |
| EcoICRI | GAGCTC | 1 cut(s) | 631 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 350 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1153 |
| EcoO65I | GGTNACC | 1 cut(s) | 1017 |
| EcoRI | GAATTC | 1 cut(s) | 1305 |
| EcoRII | CCWGG | 3 cut(s) | 162, 1392, 1438 |
| EcoT22I | ATGCAT | 1 cut(s) | 1073 |
| EcoT38I | GRGCYC | 1 cut(s) | 633 |
| FaeI | CATG | 5 cut(s) | 44, 512, 533, 1071, 1219 |
| FatI | CATG | 5 cut(s) | 40, 508, 529, 1067, 1215 |
| FbaI | TGATCA | 1 cut(s) | 382 |
| FblI | GTMKAC | 1 cut(s) | 894 |
| Fnu4HI | GCNGC | 5 cut(s) | 363, 479, 482, 754, 1511 |
| FokI | GGATG | 3 cut(s) | 98, 305, 1508 |
| FriOI | GRGCYC | 1 cut(s) | 633 |
| Fsp4HI | GCNGC | 5 cut(s) | 363, 479, 482, 754, 1511 |
| FspBI | CTAG | 6 cut(s) | 113, 143, 152, 176, 236, 666 |
| GlaI | GCGC | 1 cut(s) | 181 |
| GluI | GCNGC | 5 cut(s) | 363, 479, 482, 754, 1511 |
| GsaI | CCCAGC | 1 cut(s) | 935 |
| GsuI | CTGGAG | 2 cut(s) | 1320, 1415 |
| HaeII | RGCGCY | 1 cut(s) | 183 |
| HaeIII | GGCC | 1 cut(s) | 1351 |
| HapII | CCGG | 2 cut(s) | 771, 820 |
| HgaI | GACGC | 1 cut(s) | 809 |
| HhaI | GCGC | 1 cut(s) | 182 |
| Hin1I | GRCGYC | 2 cut(s) | 801, 1212 |
| Hin1II | CATG | 5 cut(s) | 44, 512, 533, 1071, 1219 |
| Hin6I | GCGC | 1 cut(s) | 180 |
| HinP1I | GCGC | 1 cut(s) | 180 |
| HincII | GTYRAC | 1 cut(s) | 895 |
| HindII | GTYRAC | 1 cut(s) | 895 |
| HinfI | GANTC | 4 cut(s) | 463, 910, 915, 1427 |
| HpaII | CCGG | 2 cut(s) | 771, 820 |
| HphI | GGTGA | 7 cut(s) | 377, 423, 506, 554, 1136, 1176, 1382 |
| Hpy166II | GTNNAC | 2 cut(s) | 817, 895 |
| Hpy188I | TCNGA | 6 cut(s) | 250, 721, 730, 860, 1243, 1411 |
| Hpy188III | TCNNGA | 9 cut(s) | 236, 509, 847, 1145, 1216, 1249, 1291, 1361, 1415 |
| Hpy8I | GTNNAC | 2 cut(s) | 817, 895 |
| Hpy99I | CGWCG | 3 cut(s) | 803, 977, 1389 |
| HpyAV | CCTTC | 4 cut(s) | 346, 468, 627, 726 |
| HpyCH4III | ACNGT | 4 cut(s) | 325, 814, 928, 1008 |
| HpyCH4IV | ACGT | 2 cut(s) | 1081, 1212 |
| HpyCH4V | TGCA | 7 cut(s) | 278, 501, 573, 1004, 1071, 1163, 1315 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 148, 744, 753, 1107 |
| HpyF3I | CTNAG | 5 cut(s) | 138, 249, 419, 887, 1280 |
| HpySE526I | ACGT | 2 cut(s) | 1081, 1212 |
| Hsp92I | GRCGYC | 2 cut(s) | 801, 1212 |
| Hsp92II | CATG | 5 cut(s) | 44, 512, 533, 1071, 1219 |
| HspAI | GCGC | 1 cut(s) | 180 |
| Ksp22I | TGATCA | 1 cut(s) | 382 |
| Kzo9I | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| LmnI | GCTCC | 5 cut(s) | 265, 355, 628, 699, 849 |
| Lsp1109I | GCAGC | 4 cut(s) | 349, 468, 490, 1497 |
| LweI | GCATC | 1 cut(s) | 437 |
| MaeI | CTAG | 6 cut(s) | 113, 143, 152, 176, 236, 666 |
| MaeII | ACGT | 2 cut(s) | 1081, 1212 |
| MaeIII | GTNAC | 5 cut(s) | 634, 952, 1017, 1291, 1388 |
| MalI | GATC | 4 cut(s) | 384, 1128, 1408, 1413 |
| MboI | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| MflI | RGATCY | 1 cut(s) | 1406 |
| MhlI | GDGCHC | 3 cut(s) | 633, 657, 833 |
| MlyI | GAGTC | 1 cut(s) | 1436 |
| MmeI | TCCRAC | 1 cut(s) | 567 |
| Mph1103I | ATGCAT | 1 cut(s) | 1073 |
| MseI | TTAA | 8 cut(s) | 30, 69, 338, 689, 1338, 1479, 1536, 1540 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1066 |
| MspA1I | CMGCKG | 2 cut(s) | 481, 1298 |
| MspI | CCGG | 2 cut(s) | 771, 820 |
| MspR9I | CCNGG | 4 cut(s) | 164, 771, 1394, 1440 |
| MvaI | CCWGG | 3 cut(s) | 164, 1394, 1440 |
| MvnI | CGCG | 1 cut(s) | 10 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 148, 744, 753, 1107 |
| NciI | CCSGG | 1 cut(s) | 771 |
| NdeII | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| NheI | GCTAGC | 1 cut(s) | 142 |
| NlaIII | CATG | 5 cut(s) | 44, 512, 533, 1071, 1219 |
| NlaIV | GGNNCC | 4 cut(s) | 76, 937, 1143, 1155 |
| NmeAIII | GCCGAG | 1 cut(s) | 829 |
| NmuCI | GTSAC | 4 cut(s) | 634, 952, 1291, 1388 |
| NsiI | ATGCAT | 1 cut(s) | 1073 |
| NspI | RCATGY | 1 cut(s) | 1071 |
| NspV | TTCGAA | 1 cut(s) | 913 |
| PacI | TTAATTAA | 1 cut(s) | 1540 |
| PaeR7I | CTCGAG | 1 cut(s) | 1047 |
| PagI | TCATGA | 2 cut(s) | 508, 1215 |
| PfeI | GAWTC | 3 cut(s) | 463, 910, 915 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 289 |
| PfoI | TCCNGGA | 1 cut(s) | 1438 |
| PinAI | ACCGGT | 1 cut(s) | 819 |
| PkrI | GCNGC | 5 cut(s) | 364, 480, 483, 755, 1512 |
| PleI | GAGTC | 1 cut(s) | 1435 |
| PpsI | GAGTC | 1 cut(s) | 1435 |
| PpuMI | RGGWCCY | 1 cut(s) | 1153 |
| PsiI | TTATAA | 1 cut(s) | 1271 |
| Psp124BI | GAGCTC | 1 cut(s) | 633 |
| Psp5II | RGGWCCY | 1 cut(s) | 1153 |
| Psp6I | CCWGG | 3 cut(s) | 162, 1392, 1438 |
| PspEI | GGTNACC | 1 cut(s) | 1017 |
| PspFI | CCCAGC | 1 cut(s) | 931 |
| PspGI | CCWGG | 3 cut(s) | 162, 1392, 1438 |
| PspN4I | GGNNCC | 4 cut(s) | 76, 937, 1143, 1155 |
| PspPI | GGNCC | 3 cut(s) | 640, 1153, 1349 |
| PspPPI | RGGWCCY | 1 cut(s) | 1153 |
| PspXI | VCTCGAGB | 1 cut(s) | 1047 |
| PsuI | RGATCY | 1 cut(s) | 1406 |
| PvuII | CAGCTG | 1 cut(s) | 481 |
| RsaI | GTAC | 3 cut(s) | 824, 1258, 1523 |
| RsaNI | GTAC | 3 cut(s) | 823, 1257, 1522 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1066 |
| SacI | GAGCTC | 1 cut(s) | 633 |
| SalI | GTCGAC | 1 cut(s) | 893 |
| SaqAI | TTAA | 8 cut(s) | 30, 69, 338, 689, 1338, 1479, 1536, 1540 |
| SatI | GCNGC | 5 cut(s) | 363, 479, 482, 754, 1511 |
| Sau3AI | GATC | 4 cut(s) | 382, 1126, 1406, 1411 |
| Sau96I | GGNCC | 3 cut(s) | 640, 1153, 1349 |
| SchI | GAGTC | 1 cut(s) | 1436 |
| ScrFI | CCNGG | 4 cut(s) | 164, 771, 1394, 1440 |
| SduI | GDGCHC | 3 cut(s) | 633, 657, 833 |
| SfaNI | GCATC | 1 cut(s) | 437 |
| SfcI | CTRYAG | 1 cut(s) | 347 |
| Sfr274I | CTCGAG | 1 cut(s) | 1047 |
| SfuI | TTCGAA | 1 cut(s) | 913 |
| SinI | GGWCC | 2 cut(s) | 640, 1153 |
| SlaI | CTCGAG | 1 cut(s) | 1047 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1066 |
| SmlI | CTYRAG | 2 cut(s) | 1047, 1247 |
| SmoI | CTYRAG | 2 cut(s) | 1047, 1247 |
| SsiI | CCGC | 7 cut(s) | 10, 738, 747, 753, 1015, 1296, 1404 |
| SspI | AATATT | 2 cut(s) | 336, 1195 |
| SspMI | CTAG | 6 cut(s) | 113, 143, 152, 176, 236, 666 |
| SstI | GAGCTC | 1 cut(s) | 633 |
| StyD4I | CCNGG | 4 cut(s) | 162, 769, 1392, 1438 |
| TaaI | ACNGT | 4 cut(s) | 325, 814, 928, 1008 |
| TaiI | ACGT | 2 cut(s) | 1084, 1215 |
| TaqI | TCGA | 7 cut(s) | 300, 466, 798, 894, 913, 1048, 1360 |
| TatI | WGTACW | 2 cut(s) | 1256, 1521 |
| TauI | GCSGC | 1 cut(s) | 756 |
| TfiI | GAWTC | 3 cut(s) | 463, 910, 915 |
| Tru1I | TTAA | 8 cut(s) | 30, 69, 338, 689, 1338, 1479, 1536, 1540 |
| Tru9I | TTAA | 8 cut(s) | 30, 69, 338, 689, 1338, 1479, 1536, 1540 |
| TscAI | CASTG | 4 cut(s) | 330, 840, 1013, 1068 |
| TseFI | GTSAC | 4 cut(s) | 634, 952, 1291, 1388 |
| TseI | GCWGC | 4 cut(s) | 362, 478, 481, 1510 |
| Tsp45I | GTSAC | 4 cut(s) | 634, 952, 1291, 1388 |
| TspDTI | ATGAA | 7 cut(s) | 255, 386, 497, 696, 781, 1047, 1318 |
| TspGWI | ACGGA | 2 cut(s) | 120, 653 |
| TspRI | CASTG | 4 cut(s) | 330, 840, 1013, 1068 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 289 |
| VpaK11BI | GGWCC | 2 cut(s) | 640, 1153 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 350 |
| XapI | RAATTY | 1 cut(s) | 1305 |
| XbaI | TCTAGA | 1 cut(s) | 235 |
| XceI | RCATGY | 1 cut(s) | 1071 |
| XhoI | CTCGAG | 1 cut(s) | 1047 |
| XmiI | GTMKAC | 1 cut(s) | 894 |
| XspI | CTAG | 6 cut(s) | 113, 143, 152, 176, 236, 666 |
| ZraI | GACGTC | 1 cut(s) | 1213 |
| Zsp2I | ATGCAT | 1 cut(s) | 1073 |