Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 344 |
| Acc36I | ACCTGC | 1 cut(s) | 344 |
| AccB1I | GGYRCC | 1 cut(s) | 506 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 685 |
| AccI | GTMKAC | 1 cut(s) | 1109 |
| AccII | CGCG | 3 cut(s) | 110, 133, 903 |
| AciI | CCGC | 3 cut(s) | 133, 154, 871 |
| AclI | AACGTT | 1 cut(s) | 77 |
| AclWI | GGATC | 3 cut(s) | 29, 161, 1092 |
| AcoI | YGGCCR | 1 cut(s) | 1045 |
| AfaI | GTAC | 2 cut(s) | 51, 287 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 443, 685, 745 |
| AgsI | TTSAA | 5 cut(s) | 106, 248, 363, 743, 838 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 232 |
| AjnI | CCWGG | 2 cut(s) | 312, 472 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 135, 167 |
| AluBI | AGCT | 8 cut(s) | 197, 240, 422, 630, 656, 971, 1024, 1087 |
| AluI | AGCT | 8 cut(s) | 197, 240, 422, 630, 656, 971, 1024, 1087 |
| AlwI | GGATC | 3 cut(s) | 29, 161, 1092 |
| AoxI | GGCC | 1 cut(s) | 1045 |
| ApeKI | GCWGC | 3 cut(s) | 240, 419, 874 |
| AspLEI | GCGC | 1 cut(s) | 112 |
| AspS9I | GGNCC | 1 cut(s) | 521 |
| AsuC2I | CCSGG | 2 cut(s) | 219, 349 |
| AsuHPI | GGTGA | 2 cut(s) | 207, 899 |
| AvaII | GGWCC | 1 cut(s) | 521 |
| BalI | TGGCCA | 1 cut(s) | 1047 |
| BanI | GGYRCC | 1 cut(s) | 506 |
| BbvI | GCAGC | 3 cut(s) | 227, 431, 861 |
| BccI | CCATC | 3 cut(s) | 391, 835, 1033 |
| BciT130I | CCWGG | 2 cut(s) | 314, 474 |
| BclI | TGATCA | 1 cut(s) | 775 |
| BcnI | CCSGG | 2 cut(s) | 219, 349 |
| BfaI | CTAG | 5 cut(s) | 237, 545, 596, 1014, 1059 |
| BfuAI | ACCTGC | 1 cut(s) | 344 |
| BisI | GCNGC | 5 cut(s) | 134, 241, 420, 872, 875 |
| BlsI | GCNGC | 5 cut(s) | 135, 242, 421, 873, 876 |
| Bme1390I | CCNGG | 4 cut(s) | 219, 314, 349, 474 |
| Bme18I | GGWCC | 1 cut(s) | 521 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 232 |
| BmgT120I | GGNCC | 1 cut(s) | 521 |
| BmiI | GGNNCC | 3 cut(s) | 346, 448, 508 |
| BmrFI | CCNGG | 4 cut(s) | 219, 314, 349, 474 |
| BmsI | GCATC | 1 cut(s) | 830 |
| BpuMI | CCSGG | 2 cut(s) | 219, 349 |
| Bsa29I | ATCGAT | 2 cut(s) | 64, 854 |
| BsaJI | CCNNGG | 4 cut(s) | 218, 312, 339, 348 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 135, 165 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 443, 685, 745 |
| BseBI | CCWGG | 2 cut(s) | 314, 474 |
| BseCI | ATCGAT | 2 cut(s) | 64, 854 |
| BseDI | CCNNGG | 4 cut(s) | 218, 312, 339, 348 |
| BseGI | GGATG | 1 cut(s) | 694 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 443, 685, 745 |
| BseMII | CTCAG | 3 cut(s) | 30, 129, 636 |
| BseXI | GCAGC | 3 cut(s) | 227, 431, 861 |
| Bsh1236I | CGCG | 3 cut(s) | 110, 133, 903 |
| BshFI | GGCC | 1 cut(s) | 1047 |
| BshNI | GGYRCC | 1 cut(s) | 506 |
| BshVI | ATCGAT | 2 cut(s) | 64, 854 |
| BsiSI | CCGG | 3 cut(s) | 218, 348, 413 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 443, 685, 745 |
| BsnI | GGCC | 1 cut(s) | 1047 |
| Bsp143I | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| Bsp19I | CCATGG | 1 cut(s) | 339 |
| Bsp68I | TCGCGA | 1 cut(s) | 903 |
| BspACI | CCGC | 3 cut(s) | 133, 154, 871 |
| BspANI | GGCC | 1 cut(s) | 1047 |
| BspCNI | CTCAG | 3 cut(s) | 29, 128, 637 |
| BspDI | ATCGAT | 2 cut(s) | 64, 854 |
| BspFNI | CGCG | 3 cut(s) | 110, 133, 903 |
| BspHI | TCATGA | 1 cut(s) | 778 |
| BspLI | GGNNCC | 3 cut(s) | 346, 448, 508 |
| BspMI | ACCTGC | 1 cut(s) | 344 |
| BspPI | GGATC | 3 cut(s) | 29, 161, 1092 |
| BspT107I | GGYRCC | 1 cut(s) | 506 |
| BssECI | CCNNGG | 4 cut(s) | 218, 312, 339, 348 |
| BssMI | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| BssT1I | CCWWGG | 1 cut(s) | 339 |
| Bst2UI | CCWGG | 2 cut(s) | 314, 474 |
| Bst4CI | ACNGT | 8 cut(s) | 147, 203, 371, 521, 671, 683, 721, 978 |
| Bst6I | CTCTTC | 1 cut(s) | 720 |
| BstC8I | GCNNGC | 1 cut(s) | 534 |
| BstDEI | CTNAG | 3 cut(s) | 16, 115, 645 |
| BstDSI | CCRYGG | 1 cut(s) | 339 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 743 |
| BstF5I | GGATG | 1 cut(s) | 694 |
| BstFNI | CGCG | 3 cut(s) | 110, 133, 903 |
| BstHHI | GCGC | 1 cut(s) | 112 |
| BstKTI | GATC | 7 cut(s) | 22, 37, 68, 169, 778, 854, 1100 |
| BstMBI | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 133, 900 |
| BstNI | CCWGG | 2 cut(s) | 314, 474 |
| BstSCI | CCNGG | 4 cut(s) | 217, 312, 347, 472 |
| BstUI | CGCG | 3 cut(s) | 110, 133, 903 |
| BstV1I | GCAGC | 3 cut(s) | 227, 431, 861 |
| BstX2I | RGATCY | 1 cut(s) | 1097 |
| BstYI | RGATCY | 1 cut(s) | 1097 |
| Bsu15I | ATCGAT | 2 cut(s) | 64, 854 |
| BsuRI | GGCC | 1 cut(s) | 1047 |
| BsuTUI | ATCGAT | 2 cut(s) | 64, 854 |
| BtgI | CCRYGG | 1 cut(s) | 339 |
| BtgZI | GCGATG | 1 cut(s) | 807 |
| BtsCI | GGATG | 1 cut(s) | 694 |
| BtsIMutI | CAGTG | 3 cut(s) | 676, 726, 1029 |
| BtuMI | TCGCGA | 1 cut(s) | 903 |
| BveI | ACCTGC | 1 cut(s) | 344 |
| Cac8I | GCNNGC | 1 cut(s) | 534 |
| CciI | TCATGA | 1 cut(s) | 778 |
| CfoI | GCGC | 1 cut(s) | 112 |
| Cfr13I | GGNCC | 1 cut(s) | 521 |
| ClaI | ATCGAT | 2 cut(s) | 64, 854 |
| CseI | GACGC | 1 cut(s) | 222 |
| Csp6I | GTAC | 2 cut(s) | 50, 286 |
| CviQI | GTAC | 2 cut(s) | 50, 286 |
| DdeI | CTNAG | 3 cut(s) | 16, 115, 645 |
| DpnI | GATC | 7 cut(s) | 21, 36, 67, 168, 777, 853, 1099 |
| DpnII | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| DriI | GACNNNNNGTC | 1 cut(s) | 232 |
| EaeI | YGGCCR | 1 cut(s) | 1045 |
| Eam1104I | CTCTTC | 1 cut(s) | 720 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 232 |
| EarI | CTCTTC | 1 cut(s) | 720 |
| Eco130I | CCWWGG | 1 cut(s) | 339 |
| Eco47I | GGWCC | 1 cut(s) | 521 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 743 |
| EcoRII | CCWGG | 2 cut(s) | 312, 472 |
| EcoT14I | CCWWGG | 1 cut(s) | 339 |
| EcoT22I | ATGCAT | 2 cut(s) | 640, 823 |
| ErhI | CCWWGG | 1 cut(s) | 339 |
| FbaI | TGATCA | 1 cut(s) | 775 |
| FblI | GTMKAC | 1 cut(s) | 1109 |
| Fnu4HI | GCNGC | 5 cut(s) | 134, 241, 420, 872, 875 |
| FokI | GGATG | 1 cut(s) | 701 |
| Fsp4HI | GCNGC | 5 cut(s) | 134, 241, 420, 872, 875 |
| FspBI | CTAG | 5 cut(s) | 237, 545, 596, 1014, 1059 |
| GlaI | GCGC | 1 cut(s) | 111 |
| GluI | GCNGC | 5 cut(s) | 134, 241, 420, 872, 875 |
| HaeIII | GGCC | 1 cut(s) | 1047 |
| HapII | CCGG | 3 cut(s) | 218, 348, 413 |
| HgaI | GACGC | 1 cut(s) | 222 |
| HhaI | GCGC | 1 cut(s) | 112 |
| Hin6I | GCGC | 1 cut(s) | 110 |
| HinP1I | GCGC | 1 cut(s) | 110 |
| HincII | GTYRAC | 1 cut(s) | 81 |
| HindII | GTYRAC | 1 cut(s) | 81 |
| HinfI | GANTC | 2 cut(s) | 477, 856 |
| HpaII | CCGG | 3 cut(s) | 218, 348, 413 |
| HphI | GGTGA | 2 cut(s) | 207, 899 |
| Hpy166II | GTNNAC | 3 cut(s) | 81, 667, 1110 |
| Hpy188I | TCNGA | 5 cut(s) | 19, 142, 428, 646, 660 |
| Hpy188III | TCNNGA | 8 cut(s) | 38, 437, 465, 524, 779, 860, 902, 1014 |
| Hpy8I | GTNNAC | 3 cut(s) | 81, 667, 1110 |
| Hpy99I | CGWCG | 1 cut(s) | 230 |
| HpyAV | CCTTC | 4 cut(s) | 19, 857, 861, 1020 |
| HpyCH4III | ACNGT | 8 cut(s) | 147, 203, 371, 521, 671, 683, 721, 978 |
| HpyCH4IV | ACGT | 2 cut(s) | 77, 228 |
| HpyCH4V | TGCA | 6 cut(s) | 264, 638, 821, 877, 894, 1066 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 133, 900 |
| HpyF3I | CTNAG | 3 cut(s) | 16, 115, 645 |
| HpySE526I | ACGT | 2 cut(s) | 77, 228 |
| HspAI | GCGC | 1 cut(s) | 110 |
| Ksp22I | TGATCA | 1 cut(s) | 775 |
| Kzo9I | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| LmnI | GCTCC | 1 cut(s) | 1092 |
| Lsp1109I | GCAGC | 3 cut(s) | 227, 431, 861 |
| LweI | GCATC | 1 cut(s) | 830 |
| MaeI | CTAG | 5 cut(s) | 237, 545, 596, 1014, 1059 |
| MaeII | ACGT | 2 cut(s) | 77, 228 |
| MaeIII | GTNAC | 2 cut(s) | 70, 1027 |
| MalI | GATC | 7 cut(s) | 21, 36, 67, 168, 777, 853, 1099 |
| MboI | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| MboII | GAAGA | 6 cut(s) | 406, 599, 737, 786, 850, 1088 |
| MflI | RGATCY | 1 cut(s) | 1097 |
| MlsI | TGGCCA | 1 cut(s) | 1047 |
| MluCI | AATT | 5 cut(s) | 101, 572, 801, 1079, 1103 |
| MluNI | TGGCCA | 1 cut(s) | 1047 |
| MmeI | TCCRAC | 1 cut(s) | 960 |
| MnlI | CCTC | 7 cut(s) | 105, 161, 422, 460, 574, 952, 1101 |
| Mox20I | TGGCCA | 1 cut(s) | 1047 |
| Mph1103I | ATGCAT | 2 cut(s) | 640, 823 |
| MscI | TGGCCA | 1 cut(s) | 1047 |
| MseI | TTAA | 4 cut(s) | 281, 734, 761, 1082 |
| Msp20I | TGGCCA | 1 cut(s) | 1047 |
| MspI | CCGG | 3 cut(s) | 218, 348, 413 |
| MspR9I | CCNGG | 4 cut(s) | 219, 314, 349, 474 |
| MvaI | CCWGG | 2 cut(s) | 314, 474 |
| MvnI | CGCG | 3 cut(s) | 110, 133, 903 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 133, 900 |
| NciI | CCSGG | 2 cut(s) | 219, 349 |
| NcoI | CCATGG | 1 cut(s) | 339 |
| NdeII | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| NlaIV | GGNNCC | 3 cut(s) | 346, 448, 508 |
| NmuCI | GTSAC | 2 cut(s) | 70, 1027 |
| NruI | TCGCGA | 1 cut(s) | 903 |
| NsiI | ATGCAT | 2 cut(s) | 640, 823 |
| PagI | TCATGA | 1 cut(s) | 778 |
| PaqCI | CACCTGC | 1 cut(s) | 344 |
| PfeI | GAWTC | 2 cut(s) | 477, 856 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 685 |
| PfoI | TCCNGGA | 1 cut(s) | 472 |
| PkrI | GCNGC | 5 cut(s) | 135, 242, 421, 873, 876 |
| Psp1406I | AACGTT | 1 cut(s) | 77 |
| Psp6I | CCWGG | 2 cut(s) | 312, 472 |
| PspGI | CCWGG | 2 cut(s) | 312, 472 |
| PspN4I | GGNNCC | 3 cut(s) | 346, 448, 508 |
| PspPI | GGNCC | 1 cut(s) | 521 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 659, 691 |
| PsuI | RGATCY | 1 cut(s) | 1097 |
| RruI | TCGCGA | 1 cut(s) | 903 |
| RsaI | GTAC | 2 cut(s) | 51, 287 |
| RsaNI | GTAC | 2 cut(s) | 50, 286 |
| SaqAI | TTAA | 4 cut(s) | 281, 734, 761, 1082 |
| SatI | GCNGC | 5 cut(s) | 134, 241, 420, 872, 875 |
| Sau3AI | GATC | 7 cut(s) | 19, 34, 65, 166, 775, 851, 1097 |
| Sau96I | GGNCC | 1 cut(s) | 521 |
| ScrFI | CCNGG | 4 cut(s) | 219, 314, 349, 474 |
| SfaNI | GCATC | 1 cut(s) | 830 |
| SinI | GGWCC | 1 cut(s) | 521 |
| Sse9I | AATT | 5 cut(s) | 101, 572, 801, 1079, 1103 |
| SsiI | CCGC | 3 cut(s) | 133, 154, 871 |
| SspMI | CTAG | 5 cut(s) | 237, 545, 596, 1014, 1059 |
| StyD4I | CCNGG | 4 cut(s) | 217, 312, 347, 472 |
| StyI | CCWWGG | 1 cut(s) | 339 |
| TaaI | ACNGT | 8 cut(s) | 147, 203, 371, 521, 671, 683, 721, 978 |
| TaiI | ACGT | 2 cut(s) | 80, 231 |
| TaqI | TCGA | 4 cut(s) | 64, 97, 854, 859 |
| TasI | AATT | 5 cut(s) | 101, 572, 801, 1079, 1103 |
| TatI | WGTACW | 1 cut(s) | 285 |
| TauI | GCSGC | 2 cut(s) | 136, 874 |
| TfiI | GAWTC | 2 cut(s) | 477, 856 |
| Tru1I | TTAA | 4 cut(s) | 281, 734, 761, 1082 |
| Tru9I | TTAA | 4 cut(s) | 281, 734, 761, 1082 |
| TscAI | CASTG | 3 cut(s) | 676, 726, 1036 |
| TseFI | GTSAC | 2 cut(s) | 70, 1027 |
| TseI | GCWGC | 3 cut(s) | 240, 419, 874 |
| Tsp45I | GTSAC | 2 cut(s) | 70, 1027 |
| TspDTI | ATGAA | 6 cut(s) | 309, 368, 786, 798, 827, 943 |
| TspGWI | ACGGA | 1 cut(s) | 404 |
| TspRI | CASTG | 3 cut(s) | 676, 726, 1036 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 685 |
| VpaK11BI | GGWCC | 1 cut(s) | 521 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 743 |
| XbaI | TCTAGA | 1 cut(s) | 1013 |
| XmiI | GTMKAC | 1 cut(s) | 1109 |
| XspI | CTAG | 5 cut(s) | 237, 545, 596, 1014, 1059 |
| Zsp2I | ATGCAT | 2 cut(s) | 640, 823 |