Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0000464

Physical Location & Seq

Genomic Coordinates Reverse (-)

13110921 .. 13112308

Gene Structure 1388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g15030.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1194 bp

ATGGCTTCCCTCATCTCAGATCTTCATTCCAGCGATCCCGAGATTCTGGTACGAAAGAATAATCGATTCGTGACGACGTTGACACTCAACAGGCCTCGGAAGTTGAATGCCCTCTCGGTTTCAATGCTGTCACGGCTGTCGGAGCTGTTCCTCCGTTGCGACAACGATCCCCATGTCAAGTTGGTCATTCTCAAAGCTACCGGCAAGACATTTTCACCGGGCGGCGACATTGCTTCTGTAGCTCAGCATCTCTACAATGGCAATGTGAGAGCTGCTTGCAGAAGTTTCGCAACTGCATACAGCTTAATGTACTTGGTGGCAACAAAGACTAGACCCCAGGTTTCATTTCTCAATGGAATGACCATGGGGACCGGCGCAGGTGTTTCTATACACGGTAGCTTTCGTGTCACGACGGAGAAGACGGTGTTTGCTATGCCGGAAGCAGCTATCGGAGGGTTTCCTGATGTTGGTTCCTCTTATTTCCTGTCTAGACTTCCTGGATTCTTTGGAGAGTATCTTGGTCTAACAGGTGCTAAATTGGATGGTCCTGAAATGCTTGCTTTGGGTCTAGCAACTCACTTTGTTCCCTCATCTAAATTGGCTTCGCTAGAAGAAGCCCTAGTGAGAACACTCGGTGCTTTACCTAATACAAGCTCTAGTGATGACCGGGCTTTTATTTCAGCTATTCTAGATGCATACTCGGAGCAGCCAGCTTTGAAAGCGAAGAGTGCTTACCACCGTATGGATGTCATCGACAAGTGCTTTTCTCAATCAACAGTGGAAGAAATTTTAAGTAGCCTTGAGAAGGAGTATGCTACATCAACCAACACAAGTGATCATCATGAATGGTTATCTTCATCGATTCAATCAATGAAAAGCGCATCGCCGATAAGTTTGAAGATTGCTCTGAGATCGATTCGAGGAGGAAGGAATCTGCAAGGAGTCGGTGAGTGCATTGTTCGCGAATATAGGATTATTTGTCATGTTTTGCGTGGGGAAATCAGCAAGGATTTCATGGAGGGTTGCAGAGCTATACTGTTGGACAAGGATAAGAACCCAAAGTGGGAGCCTTCTAGATTAGAGCTTGTCACTGACCATATGGTTAGCCACTACTTCTCTAGGTTGGATGATGACGAAGAATTAAAGCGCCCTCAAACATCAAAATTGTCTGTAACTCCCATTTCCAAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

398

Amino Acids

43.6

Weight (kDa)

8.62

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	23 - 269	2.2e-25	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 372	2e-112	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca	FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica	MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica	Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis	pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis	RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata	RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora	Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii	Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa	Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha	Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana	Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	368
Acc36I	ACCTGC	1 cut(s)	368
AccB7I	CCANNNNNTGG	1 cut(s)	742
AccII	CGCG	1 cut(s)	963
AciI	CCGC	1 cut(s)	222
AclWI	GGATC	2 cut(s)	29, 161
AcsI	RAATTY	1 cut(s)	786
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	2 cut(s)	51, 311
AfiI	CCNNNNNNNGG	4 cut(s)	467, 742, 805, 1063
AgsI	TTSAA	5 cut(s)	106, 123, 718, 866, 898
AjnI	CCWGG	2 cut(s)	336, 496
AlwI	GGATC	2 cut(s)	29, 161
Ama87I	CYCGRG	1 cut(s)	38
AoxI	GGCC	1 cut(s)	92
ApeKI	GCWGC	3 cut(s)	272, 443, 706
ApoI	RAATTY	1 cut(s)	786
AspLEI	GCGC	3 cut(s)	377, 881, 1149
AspS9I	GGNCC	2 cut(s)	369, 545
AsuC2I	CCSGG	2 cut(s)	219, 668
AsuHPI	GGTGA	2 cut(s)	207, 959
AvaI	CYCGRG	1 cut(s)	38
AvaII	GGWCC	2 cut(s)	369, 545
BarI	GAAGNNNNNNTAC	2 cut(s)	716, 748
BbsI	GAAGAC	1 cut(s)	425
BbvI	GCAGC	3 cut(s)	259, 455, 718
BccI	CCATC	1 cut(s)	536
BceAI	ACGGC	1 cut(s)	149
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	2 cut(s)	338, 498
BclI	TGATCA	1 cut(s)	835
BcnI	CCSGG	2 cut(s)	219, 668
BfaI	CTAG	9 cut(s)	330, 489, 569, 608, 620, 657, 689, 1074, 1119
BfmI	CTRYAG	1 cut(s)	237
BfoI	RGCGCY	1 cut(s)	1150
BfuAI	ACCTGC	1 cut(s)	368
BglII	AGATCT	1 cut(s)	19
BisI	GCNGC	4 cut(s)	223, 273, 444, 707
BlpI	GCTNAGC	1 cut(s)	243
BlsI	GCNGC	4 cut(s)	224, 274, 445, 708
Bme1390I	CCNGG	4 cut(s)	219, 338, 498, 668
Bme18I	GGWCC	2 cut(s)	369, 545
BmeT110I	CYCGRG	1 cut(s)	38
BmgT120I	GGNCC	2 cut(s)	369, 545
BmiI	GGNNCC	3 cut(s)	370, 472, 1068
BmrFI	CCNGG	4 cut(s)	219, 338, 498, 668
BmsI	GCATC	3 cut(s)	256, 682, 890
BpiI	GAAGAC	1 cut(s)	425
Bpu1102I	GCTNAGC	1 cut(s)	243
BpuEI	CTTGAG	1 cut(s)	821
BpuMI	CCSGG	2 cut(s)	219, 668
Bsa29I	ATCGAT	3 cut(s)	64, 860, 914
BsaJI	CCNNGG	3 cut(s)	95, 336, 363
Bsc4I	CCNNNNNNNGG	4 cut(s)	467, 742, 805, 1063
Bse118I	RCCGGY	2 cut(s)	200, 371
Bse3DI	GCAATG	2 cut(s)	228, 268
BseBI	CCWGG	2 cut(s)	338, 498
BseCI	ATCGAT	3 cut(s)	64, 860, 914
BseDI	CCNNGG	3 cut(s)	95, 336, 363
BseGI	GGATG	3 cut(s)	547, 751, 1132
BseLI	CCNNNNNNNGG	4 cut(s)	467, 742, 805, 1063
BseMI	GCAATG	2 cut(s)	228, 268
BseMII	CTCAG	3 cut(s)	30, 257, 899
BseRI	GAGGAG	1 cut(s)	936
BseXI	GCAGC	3 cut(s)	259, 455, 718
Bsh1236I	CGCG	1 cut(s)	963
BshFI	GGCC	1 cut(s)	94
BshVI	ATCGAT	3 cut(s)	64, 860, 914
BsiHKCI	CYCGRG	1 cut(s)	38
BsiSI	CCGG	5 cut(s)	201, 218, 372, 437, 667
BslFI	GGGAC	1 cut(s)	382
BslI	CCNNNNNNNGG	4 cut(s)	467, 742, 805, 1063
BsmFI	GGGAC	1 cut(s)	382
BsmI	GAATGC	1 cut(s)	112
BsnI	GGCC	1 cut(s)	94
BsoBI	CYCGRG	1 cut(s)	38
Bsp143I	GATC	5 cut(s)	19, 34, 166, 835, 911
Bsp1720I	GCTNAGC	1 cut(s)	243
Bsp19I	CCATGG	1 cut(s)	363
Bsp68I	TCGCGA	1 cut(s)	963
BspACI	CCGC	1 cut(s)	222
BspANI	GGCC	1 cut(s)	94
BspCNI	CTCAG	3 cut(s)	29, 256, 900
BspDI	ATCGAT	3 cut(s)	64, 860, 914
BspFNI	CGCG	1 cut(s)	963
BspHI	TCATGA	1 cut(s)	841
BspLI	GGNNCC	3 cut(s)	370, 472, 1068
BspMI	ACCTGC	1 cut(s)	368
BspPI	GGATC	2 cut(s)	29, 161
BsrDI	GCAATG	2 cut(s)	228, 268
BsrFI	RCCGGY	2 cut(s)	200, 371
BssAI	RCCGGY	2 cut(s)	200, 371
BssECI	CCNNGG	3 cut(s)	95, 336, 363
BssMI	GATC	5 cut(s)	19, 34, 166, 835, 911
BssT1I	CCWWGG	1 cut(s)	363
Bst2UI	CCWGG	2 cut(s)	338, 498
Bst4CI	ACNGT	5 cut(s)	395, 424, 740, 778, 1038
Bst6I	CTCTTC	1 cut(s)	719
BstC8I	GCNNGC	3 cut(s)	277, 558, 711
BstDEI	CTNAG	3 cut(s)	16, 243, 908
BstDSI	CCRYGG	1 cut(s)	363
BstENI	CCTNNNNNAGG	1 cut(s)	803
BstF5I	GGATG	3 cut(s)	547, 751, 1132
BstFNI	CGCG	1 cut(s)	963
BstH2I	RGCGCY	1 cut(s)	1150
BstHHI	GCGC	3 cut(s)	377, 881, 1149
BstKTI	GATC	5 cut(s)	22, 37, 169, 838, 914
BstMBI	GATC	5 cut(s)	19, 34, 166, 835, 911
BstMWI	GCNNNNNNNGC	6 cut(s)	133, 142, 239, 719, 728, 960
BstNI	CCWGG	2 cut(s)	338, 498
BstSCI	CCNGG	4 cut(s)	217, 336, 496, 666
BstSFI	CTRYAG	1 cut(s)	237
BstUI	CGCG	1 cut(s)	963
BstV1I	GCAGC	3 cut(s)	259, 455, 718
BstV2I	GAAGAC	1 cut(s)	425
BstX2I	RGATCY	1 cut(s)	19
BstYI	RGATCY	1 cut(s)	19
Bsu15I	ATCGAT	3 cut(s)	64, 860, 914
BsuRI	GGCC	1 cut(s)	94
BsuTUI	ATCGAT	3 cut(s)	64, 860, 914
BtgI	CCRYGG	1 cut(s)	363
BtgZI	GCGATG	1 cut(s)	867
BtsCI	GGATG	3 cut(s)	547, 751, 1132
BtsIMutI	CAGTG	2 cut(s)	783, 1089
BtuMI	TCGCGA	1 cut(s)	963
BveI	ACCTGC	1 cut(s)	368
Cac8I	GCNNGC	3 cut(s)	277, 558, 711
CciI	TCATGA	1 cut(s)	841
CfoI	GCGC	3 cut(s)	377, 881, 1149
Cfr10I	RCCGGY	2 cut(s)	200, 371
Cfr13I	GGNCC	2 cut(s)	369, 545
ClaI	ATCGAT	3 cut(s)	64, 860, 914
Csp6I	GTAC	2 cut(s)	50, 310
CviAII	CATG	5 cut(s)	173, 364, 842, 983, 1015
CviQI	GTAC	2 cut(s)	50, 310
DdeI	CTNAG	3 cut(s)	16, 243, 908
DpnI	GATC	5 cut(s)	21, 36, 168, 837, 913
DpnII	GATC	5 cut(s)	19, 34, 166, 835, 911
DraIII	CACNNNGTG	1 cut(s)	635
Eam1104I	CTCTTC	1 cut(s)	719
EarI	CTCTTC	1 cut(s)	719
Eco130I	CCWWGG	1 cut(s)	363
Eco147I	AGGCCT	1 cut(s)	94
Eco47I	GGWCC	2 cut(s)	369, 545
Eco88I	CYCGRG	1 cut(s)	38
EcoNI	CCTNNNNNAGG	1 cut(s)	803
EcoRII	CCWGG	2 cut(s)	336, 496
EcoT14I	CCWWGG	1 cut(s)	363
EcoT22I	ATGCAT	1 cut(s)	697
ErhI	CCWWGG	1 cut(s)	363
FaeI	CATG	5 cut(s)	176, 367, 845, 986, 1018
FaqI	GGGAC	1 cut(s)	382
FatI	CATG	5 cut(s)	172, 363, 841, 982, 1014
FauNDI	CATATG	1 cut(s)	1098
FbaI	TGATCA	1 cut(s)	835
Fnu4HI	GCNGC	4 cut(s)	223, 273, 444, 707
FokI	GGATG	3 cut(s)	554, 758, 1139
Fsp4HI	GCNGC	4 cut(s)	223, 273, 444, 707
FspBI	CTAG	9 cut(s)	330, 489, 569, 608, 620, 657, 689, 1074, 1119
GlaI	GCGC	3 cut(s)	376, 880, 1148
GluI	GCNGC	4 cut(s)	223, 273, 444, 707
HaeII	RGCGCY	1 cut(s)	1150
HaeIII	GGCC	1 cut(s)	94
HapII	CCGG	5 cut(s)	201, 218, 372, 437, 667
HhaI	GCGC	3 cut(s)	377, 881, 1149
Hin1II	CATG	5 cut(s)	176, 367, 845, 986, 1018
Hin6I	GCGC	3 cut(s)	375, 879, 1147
HinP1I	GCGC	3 cut(s)	375, 879, 1147
HincII	GTYRAC	1 cut(s)	81
HindII	GTYRAC	1 cut(s)	81
HindIII	AAGCTT	1 cut(s)	1187
HinfI	GANTC	7 cut(s)	43, 66, 501, 862, 916, 931, 942
HpaII	CCGG	5 cut(s)	201, 218, 372, 437, 667
HphI	GGTGA	2 cut(s)	207, 959
Hpy166II	GTNNAC	1 cut(s)	81
Hpy188I	TCNGA	6 cut(s)	19, 99, 142, 452, 703, 909
Hpy8I	GTNNAC	1 cut(s)	81
Hpy99I	CGWCG	2 cut(s)	79, 415
HpyAV	CCTTC	3 cut(s)	799, 921, 1080
HpyCH4III	ACNGT	5 cut(s)	395, 424, 740, 778, 1038
HpyCH4IV	ACGT	1 cut(s)	77
HpyCH4V	TGCA	6 cut(s)	279, 296, 695, 937, 954, 1026
HpyF10VI	GCNNNNNNNGC	6 cut(s)	133, 142, 239, 719, 728, 960
HpyF3I	CTNAG	3 cut(s)	16, 243, 908
HpySE526I	ACGT	1 cut(s)	77
Hsp92II	CATG	5 cut(s)	176, 367, 845, 986, 1018
HspAI	GCGC	3 cut(s)	375, 879, 1147
Ksp22I	TGATCA	1 cut(s)	835
Kzo9I	GATC	5 cut(s)	19, 34, 166, 835, 911
LmnI	GCTCC	3 cut(s)	142, 703, 1066
Lsp1109I	GCAGC	3 cut(s)	259, 455, 718
LweI	GCATC	3 cut(s)	256, 682, 890
MaeI	CTAG	9 cut(s)	330, 489, 569, 608, 620, 657, 689, 1074, 1119
MaeII	ACGT	1 cut(s)	77
MaeIII	GTNAC	5 cut(s)	70, 129, 406, 1087, 1171
MalI	GATC	5 cut(s)	21, 36, 168, 837, 913
MboI	GATC	5 cut(s)	19, 34, 166, 835, 911
MboII	GAAGA	8 cut(s)	14, 430, 623, 736, 794, 846, 910, 1148
MflI	RGATCY	1 cut(s)	19
MluCI	AATT	5 cut(s)	536, 596, 786, 1139, 1163
MlyI	GAGTC	1 cut(s)	951
MmeI	TCCRAC	3 cut(s)	120, 1020, 1104
Mph1103I	ATGCAT	1 cut(s)	697
MseI	TTAA	3 cut(s)	305, 791, 1142
MspI	CCGG	5 cut(s)	201, 218, 372, 437, 667
MspR9I	CCNGG	4 cut(s)	219, 338, 498, 668
Mva1269I	GAATGC	1 cut(s)	112
MvaI	CCWGG	2 cut(s)	338, 498
MvnI	CGCG	1 cut(s)	963
MwoI	GCNNNNNNNGC	6 cut(s)	133, 142, 239, 719, 728, 960
NciI	CCSGG	2 cut(s)	219, 668
NcoI	CCATGG	1 cut(s)	363
NdeI	CATATG	1 cut(s)	1098
NdeII	GATC	5 cut(s)	19, 34, 166, 835, 911
NlaIII	CATG	5 cut(s)	176, 367, 845, 986, 1018
NlaIV	GGNNCC	3 cut(s)	370, 472, 1068
NmuCI	GTSAC	4 cut(s)	70, 129, 406, 1087
NruI	TCGCGA	1 cut(s)	963
NsiI	ATGCAT	1 cut(s)	697
PagI	TCATGA	1 cut(s)	841
PaqCI	CACCTGC	1 cut(s)	368
PceI	AGGCCT	1 cut(s)	94
PctI	GAATGC	1 cut(s)	112
PfeI	GAWTC	6 cut(s)	43, 66, 501, 862, 916, 931
PflMI	CCANNNNNTGG	1 cut(s)	742
PfoI	TCCNGGA	1 cut(s)	496
PkrI	GCNGC	4 cut(s)	224, 274, 445, 708
PleI	GAGTC	1 cut(s)	950
PpsI	GAGTC	1 cut(s)	950
Psp6I	CCWGG	2 cut(s)	336, 496
PspGI	CCWGG	2 cut(s)	336, 496
PspN4I	GGNNCC	3 cut(s)	370, 472, 1068
PspPI	GGNCC	2 cut(s)	369, 545
PsuI	RGATCY	1 cut(s)	19
RruI	TCGCGA	1 cut(s)	963
RsaI	GTAC	2 cut(s)	51, 311
RsaNI	GTAC	2 cut(s)	50, 310
SaqAI	TTAA	3 cut(s)	305, 791, 1142
SatI	GCNGC	4 cut(s)	223, 273, 444, 707
Sau3AI	GATC	5 cut(s)	19, 34, 166, 835, 911
Sau96I	GGNCC	2 cut(s)	369, 545
SchI	GAGTC	1 cut(s)	951
ScrFI	CCNGG	4 cut(s)	219, 338, 498, 668
SfaNI	GCATC	3 cut(s)	256, 682, 890
SfcI	CTRYAG	1 cut(s)	237
SinI	GGWCC	2 cut(s)	369, 545
SmlI	CTYRAG	1 cut(s)	800
SmoI	CTYRAG	1 cut(s)	800
Sse9I	AATT	5 cut(s)	536, 596, 786, 1139, 1163
SseBI	AGGCCT	1 cut(s)	94
SsiI	CCGC	1 cut(s)	222
SspMI	CTAG	9 cut(s)	330, 489, 569, 608, 620, 657, 689, 1074, 1119
StuI	AGGCCT	1 cut(s)	94
StyD4I	CCNGG	4 cut(s)	217, 336, 496, 666
StyI	CCWWGG	1 cut(s)	363
TaaI	ACNGT	5 cut(s)	395, 424, 740, 778, 1038
TaiI	ACGT	1 cut(s)	80
TaqI	TCGA	5 cut(s)	64, 753, 860, 914, 919
TasI	AATT	5 cut(s)	536, 596, 786, 1139, 1163
TatI	WGTACW	1 cut(s)	309
TauI	GCSGC	1 cut(s)	225
TfiI	GAWTC	6 cut(s)	43, 66, 501, 862, 916, 931
Tru1I	TTAA	3 cut(s)	305, 791, 1142
Tru9I	TTAA	3 cut(s)	305, 791, 1142
TscAI	CASTG	2 cut(s)	783, 1096
TseFI	GTSAC	4 cut(s)	70, 129, 406, 1087
TseI	GCWGC	3 cut(s)	272, 443, 706
Tsp45I	GTSAC	4 cut(s)	70, 129, 406, 1087
TspDTI	ATGAA	6 cut(s)	14, 333, 846, 858, 887, 1003
TspGWI	ACGGA	2 cut(s)	143, 428
TspRI	CASTG	2 cut(s)	783, 1096
Van91I	CCANNNNNTGG	1 cut(s)	742
VpaK11BI	GGWCC	2 cut(s)	369, 545
XagI	CCTNNNNNAGG	1 cut(s)	803
XapI	RAATTY	1 cut(s)	786
XbaI	TCTAGA	3 cut(s)	488, 688, 1073
XspI	CTAG	9 cut(s)	330, 489, 569, 608, 620, 657, 689, 1074, 1119
Zsp2I	ATGCAT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.