Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 365 |
| Acc36I | ACCTGC | 1 cut(s) | 365 |
| AccB1I | GGYRCC | 1 cut(s) | 527 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 742 |
| AciI | CCGC | 1 cut(s) | 198 |
| AclWI | GGATC | 2 cut(s) | 374, 457 |
| AfaI | GTAC | 1 cut(s) | 48 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 174, 464, 621, 742, 805 |
| AgsI | TTSAA | 8 cut(s) | 13, 103, 486, 642, 866, 898, 1162, 1186 |
| AjiI | CACGTC | 1 cut(s) | 242 |
| AjnI | CCWGG | 2 cut(s) | 333, 493 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 429, 461 |
| Alw21I | GWGCWC | 1 cut(s) | 1110 |
| Alw26I | GTCTC | 2 cut(s) | 130, 259 |
| AlwI | GGATC | 2 cut(s) | 374, 457 |
| ApeKI | GCWGC | 3 cut(s) | 236, 815, 931 |
| AspLEI | GCGC | 1 cut(s) | 635 |
| AsuHPI | GGTGA | 4 cut(s) | 830, 876, 959, 1007 |
| BanI | GGYRCC | 1 cut(s) | 527 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 716, 748 |
| Bbv12I | GWGCWC | 1 cut(s) | 1110 |
| BbvI | GCAGC | 3 cut(s) | 248, 802, 943 |
| BccI | CCATC | 1 cut(s) | 533 |
| BceAI | ACGGC | 1 cut(s) | 456 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 146, 180 |
| BciT130I | CCWGG | 2 cut(s) | 335, 495 |
| BclI | TGATCA | 1 cut(s) | 835 |
| BcoDI | GTCTC | 2 cut(s) | 130, 259 |
| BfoI | RGCGCY | 1 cut(s) | 636 |
| BfuAI | ACCTGC | 1 cut(s) | 365 |
| BisI | GCNGC | 3 cut(s) | 237, 816, 932 |
| BlsI | GCNGC | 3 cut(s) | 238, 817, 933 |
| Bme1390I | CCNGG | 2 cut(s) | 335, 495 |
| BmgBI | CACGTC | 1 cut(s) | 242 |
| BmiI | GGNNCC | 1 cut(s) | 529 |
| BmrFI | CCNGG | 2 cut(s) | 335, 495 |
| BmsI | GCATC | 2 cut(s) | 154, 890 |
| BsaI | GGTCTC | 2 cut(s) | 130, 259 |
| BsaJI | CCNNGG | 2 cut(s) | 120, 333 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 174, 464, 621, 742, 805 |
| Bse118I | RCCGGY | 1 cut(s) | 215 |
| Bse3DI | GCAATG | 1 cut(s) | 80 |
| BseBI | CCWGG | 2 cut(s) | 335, 495 |
| BseDI | CCNNGG | 2 cut(s) | 120, 333 |
| BseGI | GGATG | 2 cut(s) | 544, 751 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 174, 464, 621, 742, 805 |
| BseMI | GCAATG | 1 cut(s) | 80 |
| BseMII | CTCAG | 1 cut(s) | 30 |
| BseXI | GCAGC | 3 cut(s) | 248, 802, 943 |
| BshNI | GGYRCC | 1 cut(s) | 527 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1110 |
| BsiSI | CCGG | 1 cut(s) | 216 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 174, 464, 621, 742, 805 |
| BsmAI | GTCTC | 2 cut(s) | 130, 259 |
| BsmI | GAATGC | 1 cut(s) | 109 |
| Bso31I | GGTCTC | 2 cut(s) | 130, 259 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1110 |
| Bsp143I | GATC | 4 cut(s) | 19, 366, 449, 835 |
| Bsp19I | CCATGG | 1 cut(s) | 120 |
| BspACI | CCGC | 1 cut(s) | 198 |
| BspCNI | CTCAG | 1 cut(s) | 29 |
| BspHI | TCATGA | 1 cut(s) | 961 |
| BspLI | GGNNCC | 1 cut(s) | 529 |
| BspMI | ACCTGC | 1 cut(s) | 365 |
| BspPI | GGATC | 2 cut(s) | 374, 457 |
| BspT107I | GGYRCC | 1 cut(s) | 527 |
| BspTNI | GGTCTC | 2 cut(s) | 130, 259 |
| BsrDI | GCAATG | 1 cut(s) | 80 |
| BsrFI | RCCGGY | 1 cut(s) | 215 |
| BssAI | RCCGGY | 1 cut(s) | 215 |
| BssECI | CCNNGG | 2 cut(s) | 120, 333 |
| BssMI | GATC | 4 cut(s) | 19, 366, 449, 835 |
| BssT1I | CCWWGG | 1 cut(s) | 120 |
| Bst2UI | CCWGG | 2 cut(s) | 335, 495 |
| Bst4CI | ACNGT | 1 cut(s) | 421 |
| Bst6I | CTCTTC | 1 cut(s) | 719 |
| BstC8I | GCNNGC | 2 cut(s) | 555, 813 |
| BstDEI | CTNAG | 3 cut(s) | 16, 591, 872 |
| BstDSI | CCRYGG | 1 cut(s) | 120 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 172, 803 |
| BstF5I | GGATG | 2 cut(s) | 544, 751 |
| BstH2I | RGCGCY | 1 cut(s) | 636 |
| BstHHI | GCGC | 1 cut(s) | 635 |
| BstKTI | GATC | 4 cut(s) | 22, 369, 452, 838 |
| BstMAI | GTCTC | 2 cut(s) | 130, 259 |
| BstMBI | GATC | 4 cut(s) | 19, 366, 449, 835 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 212, 236, 719, 728 |
| BstNI | CCWGG | 2 cut(s) | 335, 495 |
| BstSCI | CCNGG | 2 cut(s) | 333, 493 |
| BstV1I | GCAGC | 3 cut(s) | 248, 802, 943 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 121 |
| BtgI | CCRYGG | 1 cut(s) | 120 |
| BtrI | CACGTC | 1 cut(s) | 242 |
| BtsCI | GGATG | 2 cut(s) | 544, 751 |
| BtsI | GCAGTG | 1 cut(s) | 1173 |
| BtsIMutI | CAGTG | 2 cut(s) | 426, 1173 |
| BveI | ACCTGC | 1 cut(s) | 365 |
| Cac8I | GCNNGC | 2 cut(s) | 555, 813 |
| CciI | TCATGA | 1 cut(s) | 961 |
| CfoI | GCGC | 1 cut(s) | 635 |
| Cfr10I | RCCGGY | 1 cut(s) | 215 |
| Csp6I | GTAC | 1 cut(s) | 47 |
| CviAII | CATG | 6 cut(s) | 26, 121, 161, 389, 962, 983 |
| CviQI | GTAC | 1 cut(s) | 47 |
| DdeI | CTNAG | 3 cut(s) | 16, 591, 872 |
| DpnI | GATC | 4 cut(s) | 21, 368, 451, 837 |
| DpnII | GATC | 4 cut(s) | 19, 366, 449, 835 |
| DraI | TTTAAA | 1 cut(s) | 286 |
| Eam1104I | CTCTTC | 1 cut(s) | 719 |
| EarI | CTCTTC | 1 cut(s) | 719 |
| Eco130I | CCWWGG | 1 cut(s) | 120 |
| Eco31I | GGTCTC | 2 cut(s) | 130, 259 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 172, 803 |
| EcoRII | CCWGG | 2 cut(s) | 333, 493 |
| EcoT14I | CCWWGG | 1 cut(s) | 120 |
| ErhI | CCWWGG | 1 cut(s) | 120 |
| FaeI | CATG | 6 cut(s) | 29, 124, 164, 392, 965, 986 |
| FatI | CATG | 6 cut(s) | 25, 120, 160, 388, 961, 982 |
| FbaI | TGATCA | 1 cut(s) | 835 |
| Fnu4HI | GCNGC | 3 cut(s) | 237, 816, 932 |
| FokI | GGATG | 2 cut(s) | 551, 758 |
| Fsp4HI | GCNGC | 3 cut(s) | 237, 816, 932 |
| GlaI | GCGC | 1 cut(s) | 634 |
| GluI | GCNGC | 3 cut(s) | 237, 816, 932 |
| HaeII | RGCGCY | 1 cut(s) | 636 |
| HapII | CCGG | 1 cut(s) | 216 |
| HhaI | GCGC | 1 cut(s) | 635 |
| Hin1II | CATG | 6 cut(s) | 29, 124, 164, 392, 965, 986 |
| Hin6I | GCGC | 1 cut(s) | 633 |
| HinP1I | GCGC | 1 cut(s) | 633 |
| HindIII | AAGCTT | 2 cut(s) | 204, 394 |
| HinfI | GANTC | 5 cut(s) | 31, 40, 354, 498, 916 |
| HpaII | CCGG | 1 cut(s) | 216 |
| HphI | GGTGA | 4 cut(s) | 830, 876, 959, 1007 |
| Hpy188I | TCNGA | 3 cut(s) | 19, 547, 703 |
| Hpy188III | TCNNGA | 6 cut(s) | 35, 458, 486, 689, 962, 1186 |
| HpyAV | CCTTC | 3 cut(s) | 799, 921, 1080 |
| HpyCH4III | ACNGT | 1 cut(s) | 421 |
| HpyCH4IV | ACGT | 1 cut(s) | 241 |
| HpyCH4V | TGCA | 5 cut(s) | 85, 236, 293, 954, 1026 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 212, 236, 719, 728 |
| HpyF3I | CTNAG | 3 cut(s) | 16, 591, 872 |
| HpySE526I | ACGT | 1 cut(s) | 241 |
| Hsp92II | CATG | 6 cut(s) | 29, 124, 164, 392, 965, 986 |
| HspAI | GCGC | 1 cut(s) | 633 |
| Ksp22I | TGATCA | 1 cut(s) | 835 |
| Kzo9I | GATC | 4 cut(s) | 19, 366, 449, 835 |
| LmnI | GCTCC | 4 cut(s) | 718, 808, 1081, 1152 |
| Lsp1109I | GCAGC | 3 cut(s) | 248, 802, 943 |
| LweI | GCATC | 2 cut(s) | 154, 890 |
| MaeII | ACGT | 1 cut(s) | 241 |
| MaeIII | GTNAC | 2 cut(s) | 1087, 1171 |
| MalI | GATC | 4 cut(s) | 21, 368, 451, 837 |
| MboI | GATC | 4 cut(s) | 19, 366, 449, 835 |
| MboII | GAAGA | 8 cut(s) | 462, 620, 630, 736, 794, 910, 1139, 1148 |
| MhlI | GDGCHC | 1 cut(s) | 1110 |
| MluCI | AATT | 8 cut(s) | 98, 259, 278, 861, 866, 1076, 1139, 1162 |
| MmeI | TCCRAC | 1 cut(s) | 1020 |
| MnlI | CCTC | 6 cut(s) | 102, 158, 720, 914, 1012, 1161 |
| MseI | TTAA | 5 cut(s) | 189, 285, 348, 791, 1142 |
| MslI | CAYNNNNRTG | 1 cut(s) | 119 |
| MspI | CCGG | 1 cut(s) | 216 |
| MspR9I | CCNGG | 2 cut(s) | 335, 495 |
| Mva1269I | GAATGC | 1 cut(s) | 109 |
| MvaI | CCWGG | 2 cut(s) | 335, 495 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 212, 236, 719, 728 |
| NcoI | CCATGG | 1 cut(s) | 120 |
| NdeII | GATC | 4 cut(s) | 19, 366, 449, 835 |
| NlaIII | CATG | 6 cut(s) | 29, 124, 164, 392, 965, 986 |
| NlaIV | GGNNCC | 1 cut(s) | 529 |
| NmuCI | GTSAC | 2 cut(s) | 1087, 1171 |
| PagI | TCATGA | 1 cut(s) | 961 |
| PaqCI | CACCTGC | 1 cut(s) | 365 |
| PctI | GAATGC | 1 cut(s) | 109 |
| PfeI | GAWTC | 5 cut(s) | 31, 40, 354, 498, 916 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 742 |
| PfoI | TCCNGGA | 1 cut(s) | 493 |
| PkrI | GCNGC | 3 cut(s) | 238, 817, 933 |
| Psp6I | CCWGG | 2 cut(s) | 333, 493 |
| PspGI | CCWGG | 2 cut(s) | 333, 493 |
| PspN4I | GGNNCC | 1 cut(s) | 529 |
| RsaI | GTAC | 1 cut(s) | 48 |
| RsaNI | GTAC | 1 cut(s) | 47 |
| RseI | CAYNNNNRTG | 1 cut(s) | 119 |
| SaqAI | TTAA | 5 cut(s) | 189, 285, 348, 791, 1142 |
| SatI | GCNGC | 3 cut(s) | 237, 816, 932 |
| Sau3AI | GATC | 4 cut(s) | 19, 366, 449, 835 |
| ScrFI | CCNGG | 2 cut(s) | 335, 495 |
| SduI | GDGCHC | 1 cut(s) | 1110 |
| SfaNI | GCATC | 2 cut(s) | 154, 890 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 119 |
| Sse9I | AATT | 8 cut(s) | 98, 259, 278, 861, 866, 1076, 1139, 1162 |
| SsiI | CCGC | 1 cut(s) | 198 |
| SspI | AATATT | 1 cut(s) | 789 |
| StyD4I | CCNGG | 2 cut(s) | 333, 493 |
| StyI | CCWWGG | 1 cut(s) | 120 |
| TaaI | ACNGT | 1 cut(s) | 421 |
| TaiI | ACGT | 1 cut(s) | 244 |
| TaqI | TCGA | 2 cut(s) | 753, 919 |
| TasI | AATT | 8 cut(s) | 98, 259, 278, 861, 866, 1076, 1139, 1162 |
| TatI | WGTACW | 1 cut(s) | 46 |
| TfiI | GAWTC | 5 cut(s) | 31, 40, 354, 498, 916 |
| Tru1I | TTAA | 5 cut(s) | 189, 285, 348, 791, 1142 |
| Tru9I | TTAA | 5 cut(s) | 189, 285, 348, 791, 1142 |
| TscAI | CASTG | 2 cut(s) | 426, 1180 |
| TseFI | GTSAC | 2 cut(s) | 1087, 1171 |
| TseI | GCWGC | 3 cut(s) | 236, 815, 931 |
| Tsp45I | GTSAC | 2 cut(s) | 1087, 1171 |
| TspDTI | ATGAA | 5 cut(s) | 55, 330, 708, 839, 950 |
| TspGWI | ACGGA | 2 cut(s) | 425, 573 |
| TspRI | CASTG | 2 cut(s) | 426, 1180 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 742 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 172, 803 |
| XbaI | TCTAGA | 1 cut(s) | 688 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 989 |