Belongs to the enoyl-CoA hydratase isomerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 776 |
| AccB1I | GGYRCC | 2 cut(s) | 470, 530 |
| AccII | CGCG | 1 cut(s) | 27 |
| AciI | CCGC | 1 cut(s) | 233 |
| AclWI | GGATC | 1 cut(s) | 675 |
| AcsI | RAATTY | 2 cut(s) | 418, 762 |
| AcuI | CTGAAG | 1 cut(s) | 878 |
| AfaI | GTAC | 2 cut(s) | 54, 1070 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 467, 594, 781, 1021 |
| AgsI | TTSAA | 9 cut(s) | 88, 122, 345, 611, 636, 656, 827, 859, 1126 |
| AjnI | CCWGG | 2 cut(s) | 488, 496 |
| AluBI | AGCT | 7 cut(s) | 446, 659, 689, 989, 1042, 1111, 1153 |
| AluI | AGCT | 7 cut(s) | 446, 659, 689, 989, 1042, 1111, 1153 |
| Alw21I | GWGCWC | 1 cut(s) | 1044 |
| AlwI | GGATC | 1 cut(s) | 675 |
| Ama87I | CYCGRG | 1 cut(s) | 776 |
| AoxI | GGCC | 1 cut(s) | 92 |
| ApeKI | GCWGC | 2 cut(s) | 679, 788 |
| ApoI | RAATTY | 2 cut(s) | 418, 762 |
| AseI | ATTAAT | 1 cut(s) | 252 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 934 |
| AsuHPI | GGTGA | 3 cut(s) | 196, 236, 837 |
| AsuII | TTCGAA | 1 cut(s) | 289 |
| AvaI | CYCGRG | 1 cut(s) | 776 |
| BanI | GGYRCC | 2 cut(s) | 470, 530 |
| BanII | GRGCYC | 1 cut(s) | 1044 |
| BbsI | GAAGAC | 1 cut(s) | 1107 |
| Bbv12I | GWGCWC | 1 cut(s) | 1044 |
| BbvI | GCAGC | 2 cut(s) | 691, 775 |
| BccI | CCATC | 3 cut(s) | 395, 536, 712 |
| BciT130I | CCWGG | 2 cut(s) | 490, 498 |
| BclI | TGATCA | 1 cut(s) | 1051 |
| BfaI | CTAG | 1 cut(s) | 744 |
| BglII | AGATCT | 1 cut(s) | 616 |
| BisI | GCNGC | 3 cut(s) | 234, 680, 789 |
| BlsI | GCNGC | 3 cut(s) | 235, 681, 790 |
| BmcAI | AGTACT | 1 cut(s) | 1070 |
| Bme1390I | CCNGG | 2 cut(s) | 490, 498 |
| BmeT110I | CYCGRG | 1 cut(s) | 776 |
| BmiI | GGNNCC | 4 cut(s) | 472, 532, 1013, 1026 |
| BmrFI | CCNGG | 2 cut(s) | 490, 498 |
| BmsI | GCATC | 3 cut(s) | 157, 691, 851 |
| BpiI | GAAGAC | 1 cut(s) | 1107 |
| Bpu14I | TTCGAA | 1 cut(s) | 289 |
| BpuEI | CTTGAG | 1 cut(s) | 176 |
| BsaJI | CCNNGG | 2 cut(s) | 95, 391 |
| BsaWI | WCCGGW | 1 cut(s) | 714 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 259, 289, 501, 531 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 467, 594, 781, 1021 |
| Bse3DI | GCAATG | 2 cut(s) | 438, 1143 |
| BseBI | CCWGG | 2 cut(s) | 490, 498 |
| BseDI | CCNNGG | 2 cut(s) | 95, 391 |
| BseGI | GGATG | 7 cut(s) | 172, 387, 547, 682, 723, 727, 1090 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 467, 594, 781, 1021 |
| BseMI | GCAATG | 2 cut(s) | 438, 1143 |
| BseMII | CTCAG | 1 cut(s) | 405 |
| BseRI | GAGGAG | 4 cut(s) | 176, 794, 797, 966 |
| BseXI | GCAGC | 2 cut(s) | 691, 775 |
| Bsh1236I | CGCG | 1 cut(s) | 27 |
| BshFI | GGCC | 1 cut(s) | 94 |
| BshNI | GGYRCC | 2 cut(s) | 470, 530 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1044 |
| BsiHKCI | CYCGRG | 1 cut(s) | 776 |
| BsiSI | CCGG | 1 cut(s) | 715 |
| BslFI | GGGAC | 1 cut(s) | 465 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 467, 594, 781, 1021 |
| BsmFI | GGGAC | 1 cut(s) | 465 |
| BsmI | GAATGC | 1 cut(s) | 112 |
| BsnI | GGCC | 1 cut(s) | 94 |
| BsoBI | CYCGRG | 1 cut(s) | 776 |
| Bsp119I | TTCGAA | 1 cut(s) | 289 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1044 |
| Bsp143I | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| Bsp19I | CCATGG | 1 cut(s) | 391 |
| BspACI | CCGC | 1 cut(s) | 233 |
| BspANI | GGCC | 1 cut(s) | 94 |
| BspCNI | CTCAG | 1 cut(s) | 406 |
| BspFNI | CGCG | 1 cut(s) | 27 |
| BspLI | GGNNCC | 4 cut(s) | 472, 532, 1013, 1026 |
| BspPI | GGATC | 1 cut(s) | 675 |
| BspQI | GCTCTTC | 1 cut(s) | 199 |
| BspT104I | TTCGAA | 1 cut(s) | 289 |
| BspT107I | GGYRCC | 2 cut(s) | 470, 530 |
| BsrDI | GCAATG | 2 cut(s) | 438, 1143 |
| BssECI | CCNNGG | 2 cut(s) | 95, 391 |
| BssMI | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| BssT1I | CCWWGG | 1 cut(s) | 391 |
| Bst2UI | CCWGG | 2 cut(s) | 490, 498 |
| Bst4CI | ACNGT | 3 cut(s) | 138, 329, 754 |
| Bst6I | CTCTTC | 2 cut(s) | 199, 1086 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 215 |
| BstBI | TTCGAA | 1 cut(s) | 289 |
| BstC8I | GCNNGC | 1 cut(s) | 558 |
| BstDEI | CTNAG | 2 cut(s) | 414, 1077 |
| BstDSI | CCRYGG | 1 cut(s) | 391 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 592, 779 |
| BstF5I | GGATG | 7 cut(s) | 172, 387, 547, 682, 723, 727, 1090 |
| BstFNI | CGCG | 1 cut(s) | 27 |
| BstKTI | GATC | 4 cut(s) | 619, 670, 875, 1054 |
| BstMBI | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 215, 797 |
| BstNI | CCWGG | 2 cut(s) | 490, 498 |
| BstSCI | CCNGG | 2 cut(s) | 488, 496 |
| BstUI | CGCG | 1 cut(s) | 27 |
| BstV1I | GCAGC | 2 cut(s) | 691, 775 |
| BstV2I | GAAGAC | 1 cut(s) | 1107 |
| BstX2I | RGATCY | 1 cut(s) | 616 |
| BstYI | RGATCY | 1 cut(s) | 616 |
| BsuRI | GGCC | 1 cut(s) | 94 |
| BtgI | CCRYGG | 1 cut(s) | 391 |
| BtsCI | GGATG | 7 cut(s) | 172, 387, 547, 682, 723, 727, 1090 |
| BtsIMutI | CAGTG | 1 cut(s) | 759 |
| Cac8I | GCNNGC | 1 cut(s) | 558 |
| CseI | GACGC | 1 cut(s) | 33 |
| Csp6I | GTAC | 2 cut(s) | 53, 1069 |
| CviAII | CATG | 3 cut(s) | 364, 392, 941 |
| CviQI | GTAC | 2 cut(s) | 53, 1069 |
| DdeI | CTNAG | 2 cut(s) | 414, 1077 |
| DpnI | GATC | 4 cut(s) | 618, 669, 874, 1053 |
| DpnII | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| Eam1104I | CTCTTC | 2 cut(s) | 199, 1086 |
| EarI | CTCTTC | 2 cut(s) | 199, 1086 |
| Ecl136II | GAGCTC | 1 cut(s) | 1042 |
| Eco130I | CCWWGG | 1 cut(s) | 391 |
| Eco147I | AGGCCT | 1 cut(s) | 94 |
| Eco24I | GRGCYC | 1 cut(s) | 1044 |
| Eco53kI | GAGCTC | 1 cut(s) | 1042 |
| Eco57I | CTGAAG | 1 cut(s) | 878 |
| Eco88I | CYCGRG | 1 cut(s) | 776 |
| EcoICRI | GAGCTC | 1 cut(s) | 1042 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 592, 779 |
| EcoRI | GAATTC | 1 cut(s) | 418 |
| EcoRII | CCWGG | 2 cut(s) | 488, 496 |
| EcoT14I | CCWWGG | 1 cut(s) | 391 |
| EcoT38I | GRGCYC | 1 cut(s) | 1044 |
| ErhI | CCWWGG | 1 cut(s) | 391 |
| FaeI | CATG | 3 cut(s) | 367, 395, 944 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 876, 908 |
| FaqI | GGGAC | 1 cut(s) | 465 |
| FatI | CATG | 3 cut(s) | 363, 391, 940 |
| FauNDI | CATATG | 2 cut(s) | 157, 1056 |
| FbaI | TGATCA | 1 cut(s) | 1051 |
| Fnu4HI | GCNGC | 3 cut(s) | 234, 680, 789 |
| FokI | GGATG | 7 cut(s) | 179, 374, 554, 669, 730, 734, 1097 |
| FriOI | GRGCYC | 1 cut(s) | 1044 |
| Fsp4HI | GCNGC | 3 cut(s) | 234, 680, 789 |
| FspBI | CTAG | 1 cut(s) | 744 |
| GluI | GCNGC | 3 cut(s) | 234, 680, 789 |
| HaeIII | GGCC | 1 cut(s) | 94 |
| HapII | CCGG | 1 cut(s) | 715 |
| HgaI | GACGC | 1 cut(s) | 33 |
| Hin1II | CATG | 3 cut(s) | 367, 395, 944 |
| HincII | GTYRAC | 1 cut(s) | 81 |
| HindII | GTYRAC | 1 cut(s) | 81 |
| HindIII | AAGCTT | 1 cut(s) | 1151 |
| HinfI | GANTC | 6 cut(s) | 38, 187, 399, 501, 632, 1122 |
| HpaII | CCGG | 1 cut(s) | 715 |
| HphI | GGTGA | 3 cut(s) | 196, 236, 837 |
| Hpy166II | GTNNAC | 4 cut(s) | 81, 300, 332, 645 |
| Hpy188I | TCNGA | 2 cut(s) | 351, 974 |
| Hpy188III | TCNNGA | 6 cut(s) | 132, 245, 461, 608, 744, 920 |
| Hpy8I | GTNNAC | 4 cut(s) | 81, 300, 332, 645 |
| Hpy99I | CGWCG | 4 cut(s) | 27, 32, 35, 38 |
| HpyAV | CCTTC | 4 cut(s) | 251, 878, 882, 1038 |
| HpyCH4III | ACNGT | 3 cut(s) | 138, 329, 754 |
| HpyCH4IV | ACGT | 1 cut(s) | 77 |
| HpyCH4V | TGCA | 9 cut(s) | 155, 278, 410, 572, 624, 791, 984, 1118, 1148 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 215, 797 |
| HpyF3I | CTNAG | 2 cut(s) | 414, 1077 |
| HpySE526I | ACGT | 1 cut(s) | 77 |
| Hsp92II | CATG | 3 cut(s) | 367, 395, 944 |
| Ksp22I | TGATCA | 1 cut(s) | 1051 |
| Kzo9I | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| LguI | GCTCTTC | 1 cut(s) | 199 |
| LmnI | GCTCC | 3 cut(s) | 1024, 1039, 1116 |
| Lsp1109I | GCAGC | 2 cut(s) | 691, 775 |
| LweI | GCATC | 3 cut(s) | 157, 691, 851 |
| MaeI | CTAG | 1 cut(s) | 744 |
| MaeII | ACGT | 1 cut(s) | 77 |
| MaeIII | GTNAC | 1 cut(s) | 1135 |
| MalI | GATC | 4 cut(s) | 618, 669, 874, 1053 |
| MboI | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| MboII | GAAGA | 6 cut(s) | 216, 759, 770, 871, 1103, 1112 |
| MflI | RGATCY | 1 cut(s) | 616 |
| MhlI | GDGCHC | 1 cut(s) | 1044 |
| MmeI | TCCRAC | 3 cut(s) | 120, 978, 1062 |
| MroXI | GAANNNNTTC | 1 cut(s) | 934 |
| MseI | TTAA | 5 cut(s) | 114, 252, 305, 314, 1106 |
| MspI | CCGG | 1 cut(s) | 715 |
| MspR9I | CCNGG | 2 cut(s) | 490, 498 |
| Mva1269I | GAATGC | 1 cut(s) | 112 |
| MvaI | CCWGG | 2 cut(s) | 490, 498 |
| MvnI | CGCG | 1 cut(s) | 27 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 215, 797 |
| NcoI | CCATGG | 1 cut(s) | 391 |
| NdeI | CATATG | 2 cut(s) | 157, 1056 |
| NdeII | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| NlaIII | CATG | 3 cut(s) | 367, 395, 944 |
| NlaIV | GGNNCC | 4 cut(s) | 472, 532, 1013, 1026 |
| NmeAIII | GCCGAG | 1 cut(s) | 76 |
| NspV | TTCGAA | 1 cut(s) | 289 |
| PaeR7I | CTCGAG | 1 cut(s) | 776 |
| PceI | AGGCCT | 1 cut(s) | 94 |
| PciSI | GCTCTTC | 1 cut(s) | 199 |
| PctI | GAATGC | 1 cut(s) | 112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 934 |
| PfeI | GAWTC | 6 cut(s) | 38, 187, 399, 501, 632, 1122 |
| PfoI | TCCNGGA | 1 cut(s) | 496 |
| PkrI | GCNGC | 3 cut(s) | 235, 681, 790 |
| PshBI | ATTAAT | 1 cut(s) | 252 |
| Psp124BI | GAGCTC | 1 cut(s) | 1044 |
| Psp6I | CCWGG | 2 cut(s) | 488, 496 |
| PspGI | CCWGG | 2 cut(s) | 488, 496 |
| PspN4I | GGNNCC | 4 cut(s) | 472, 532, 1013, 1026 |
| PspXI | VCTCGAGB | 1 cut(s) | 776 |
| PsuI | RGATCY | 1 cut(s) | 616 |
| RsaI | GTAC | 2 cut(s) | 54, 1070 |
| RsaNI | GTAC | 2 cut(s) | 53, 1069 |
| SacI | GAGCTC | 1 cut(s) | 1044 |
| SapI | GCTCTTC | 1 cut(s) | 199 |
| SaqAI | TTAA | 5 cut(s) | 114, 252, 305, 314, 1106 |
| SatI | GCNGC | 3 cut(s) | 234, 680, 789 |
| Sau3AI | GATC | 4 cut(s) | 616, 667, 872, 1051 |
| ScaI | AGTACT | 1 cut(s) | 1070 |
| ScrFI | CCNGG | 2 cut(s) | 490, 498 |
| SduI | GDGCHC | 1 cut(s) | 1044 |
| SfaNI | GCATC | 3 cut(s) | 157, 691, 851 |
| Sfr274I | CTCGAG | 1 cut(s) | 776 |
| SfuI | TTCGAA | 1 cut(s) | 289 |
| SlaI | CTCGAG | 1 cut(s) | 776 |
| SmlI | CTYRAG | 2 cut(s) | 191, 776 |
| SmoI | CTYRAG | 2 cut(s) | 191, 776 |
| SseBI | AGGCCT | 1 cut(s) | 94 |
| SsiI | CCGC | 1 cut(s) | 233 |
| SspMI | CTAG | 1 cut(s) | 744 |
| SstI | GAGCTC | 1 cut(s) | 1044 |
| StuI | AGGCCT | 1 cut(s) | 94 |
| StyD4I | CCNGG | 2 cut(s) | 488, 496 |
| StyI | CCWWGG | 1 cut(s) | 391 |
| TaaI | ACNGT | 3 cut(s) | 138, 329, 754 |
| TaiI | ACGT | 1 cut(s) | 80 |
| TaqI | TCGA | 4 cut(s) | 133, 289, 777, 815 |
| TatI | WGTACW | 1 cut(s) | 1068 |
| TauI | GCSGC | 1 cut(s) | 236 |
| TfiI | GAWTC | 6 cut(s) | 38, 187, 399, 501, 632, 1122 |
| Tru1I | TTAA | 5 cut(s) | 114, 252, 305, 314, 1106 |
| Tru9I | TTAA | 5 cut(s) | 114, 252, 305, 314, 1106 |
| TscAI | CASTG | 1 cut(s) | 759 |
| TseI | GCWGC | 2 cut(s) | 679, 788 |
| TspDTI | ATGAA | 2 cut(s) | 270, 1104 |
| TspGWI | ACGGA | 1 cut(s) | 392 |
| TspRI | CASTG | 1 cut(s) | 759 |
| VspI | ATTAAT | 1 cut(s) | 252 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 592, 779 |
| XapI | RAATTY | 2 cut(s) | 418, 762 |
| XbaI | TCTAGA | 1 cut(s) | 743 |
| XhoI | CTCGAG | 1 cut(s) | 776 |
| XmnI | GAANNNNTTC | 1 cut(s) | 934 |
| XspI | CTAG | 1 cut(s) | 744 |
| ZrmI | AGTACT | 1 cut(s) | 1070 |