Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000464

Physical Location & Seq

Genomic Coordinates Forward (+)

24405372 .. 24406304

Gene Structure 933 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1169400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGCTCTCTCGACTCTCCCAACTGTTCCTGGCGTATGACAACAGTTCCAATGTCAAGTTGATCATTCTCAAAAGTAACGGAAGAGCATTTTGTGCCGGAGGTGACGTAGCTGCGGTGCACCGTCATTTTCTTAAAGGAAATTTGAAATTTGGTGCAATAACTAGTCAGAAGATGCTTACCTTAACCTACTTTGTTTCAATTCTTAATGGGATTGCGATGGGAGGTGGCGTCGGTATTTCAATACACAGTAAATTCCGTGTAGCAACAGATAATTCAGTCTTTGCAATGCCGGAAACAGCTCTGGGATTGTTTCCCGATGTCGGTGCATCTTATTTCTTGTCAAGACTTCCTGGATTTTTTGGTGAGTATTTGGGTCTTACAGGTGCAAGATTGAATGGTGCTGAAATGCTTGCATGTGGTCTAGCGACTCACTATGTGCCCTCAGCCAAATTGTCTTCACTGGAAGAAGCTCTAAAATGCCGAGTAGCTTCATCTACTTCATCAAGCTGTGATCTTGCTTCTACTATTTCAGCAATTATAGATGAATACTCGCTGAAGAAACCAGCTTTGAGTGAAAAAAGTGCTTATTACAAAAGAGATAAGCTTATGATTTTATGGTGTTTCCAATACCTAACTATATATCGTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

217

Amino Acids

23.48

Weight (kDa)

9.27

Isoelectric Point (pI)

32.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	1 - 208	1.3e-62	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	4 - 161	8e-21	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca	FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica	MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica	Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis	pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis	RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata	RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora	Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii	Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa	Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha	Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana	Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	113
AcsI	RAATTY	3 cut(s)	139, 146, 251
AcuI	CTGAAG	1 cut(s)	575
AcyI	GRCGYC	1 cut(s)	228
AdeI	CACNNNGTG	1 cut(s)	436
AfiI	CCNNNNNNNGG	1 cut(s)	320
AgsI	TTSAA	4 cut(s)	145, 198, 240, 394
AhlI	ACTAGT	1 cut(s)	161
AjnI	CCWGG	2 cut(s)	27, 349
AluBI	AGCT	7 cut(s)	110, 299, 470, 488, 507, 566, 604
AluI	AGCT	7 cut(s)	110, 299, 470, 488, 507, 566, 604
Alw21I	GWGCWC	1 cut(s)	120
Alw44I	GTGCAC	1 cut(s)	116
ApaLI	GTGCAC	1 cut(s)	116
ApeKI	GCWGC	1 cut(s)	110
ApoI	RAATTY	3 cut(s)	139, 146, 251
AsuHPI	GGTGA	2 cut(s)	113, 374
BaeGI	GKGCMC	2 cut(s)	120, 441
BbsI	GAAGAC	1 cut(s)	447
Bbv12I	GWGCWC	1 cut(s)	120
BbvCI	CCTCAGC	1 cut(s)	442
BbvI	GCAGC	1 cut(s)	97
BccI	CCATC	1 cut(s)	211
BcgI	CGANNNNNNTGC	2 cut(s)	305, 339
BciT130I	CCWGG	2 cut(s)	29, 351
BclI	TGATCA	1 cut(s)	60
BcuI	ACTAGT	1 cut(s)	161
BfaI	CTAG	2 cut(s)	162, 422
BisI	GCNGC	1 cut(s)	111
BlsI	GCNGC	1 cut(s)	112
Bme1390I	CCNGG	2 cut(s)	29, 351
BmrFI	CCNGG	2 cut(s)	29, 351
BmsI	GCATC	2 cut(s)	162, 335
BpiI	GAAGAC	1 cut(s)	447
Bpu10I	CCTNAGC	1 cut(s)	442
BsaHI	GRCGYC	1 cut(s)	228
BsaXI	ACNNNNNCTCC	4 cut(s)	90, 120, 213, 243
Bsc4I	CCNNNNNNNGG	1 cut(s)	320
Bse1I	ACTGG	1 cut(s)	465
Bse3DI	GCAATG	1 cut(s)	291
BseBI	CCWGG	2 cut(s)	29, 351
BseLI	CCNNNNNNNGG	1 cut(s)	320
BseMI	GCAATG	1 cut(s)	291
BseMII	CTCAG	1 cut(s)	456
BseNI	ACTGG	1 cut(s)	465
BseSI	GKGCMC	2 cut(s)	120, 441
BseXI	GCAGC	1 cut(s)	97
BsiHKAI	GWGCWC	1 cut(s)	120
BsiSI	CCGG	2 cut(s)	96, 290
BslI	CCNNNNNNNGG	1 cut(s)	320
Bsp1286I	GDGCHC	2 cut(s)	120, 441
Bsp143I	GATC	2 cut(s)	60, 511
BspACI	CCGC	1 cut(s)	113
BspCNI	CTCAG	1 cut(s)	455
BspQI	GCTCTTC	1 cut(s)	76
BsrDI	GCAATG	1 cut(s)	291
BsrI	ACTGG	1 cut(s)	465
BssMI	GATC	2 cut(s)	60, 511
BssNI	GRCGYC	1 cut(s)	228
Bst2UI	CCWGG	2 cut(s)	29, 351
Bst4CI	ACNGT	4 cut(s)	24, 44, 122, 248
Bst6I	CTCTTC	1 cut(s)	76
BstACI	GRCGYC	1 cut(s)	228
BstAPI	GCANNNNNTGC	1 cut(s)	92
BstC8I	GCNNGC	1 cut(s)	411
BstDEI	CTNAG	1 cut(s)	442
BstKTI	GATC	2 cut(s)	63, 514
BstMBI	GATC	2 cut(s)	60, 511
BstMWI	GCNNNNNNNGC	1 cut(s)	92
BstNI	CCWGG	2 cut(s)	29, 351
BstNSI	RCATGY	1 cut(s)	417
BstSCI	CCNGG	2 cut(s)	27, 349
BstSLI	GKGCMC	2 cut(s)	120, 441
BstV1I	GCAGC	1 cut(s)	97
BstV2I	GAAGAC	1 cut(s)	447
BtgZI	GCGATG	1 cut(s)	230
BtsIMutI	CAGTG	1 cut(s)	458
Cac8I	GCNNGC	1 cut(s)	411
CseI	GACGC	1 cut(s)	217
CviAII	CATG	1 cut(s)	414
CviJI	RGCY	8 cut(s)	110, 299, 446, 470, 488, 507, 566, 604
CviKI_1	RGCY	8 cut(s)	110, 299, 446, 470, 488, 507, 566, 604
DdeI	CTNAG	1 cut(s)	442
DpnI	GATC	2 cut(s)	62, 513
DpnII	GATC	2 cut(s)	60, 511
DraIII	CACNNNGTG	1 cut(s)	436
Eam1104I	CTCTTC	1 cut(s)	76
EarI	CTCTTC	1 cut(s)	76
Eco57I	CTGAAG	1 cut(s)	575
EcoRII	CCWGG	2 cut(s)	27, 349
FaeI	CATG	1 cut(s)	417
FaiI	YATR	8 cut(s)	36, 415, 435, 539, 608, 616, 638, 640
FatI	CATG	1 cut(s)	413
FbaI	TGATCA	1 cut(s)	60
Fnu4HI	GCNGC	1 cut(s)	111
Fsp4HI	GCNGC	1 cut(s)	111
FspBI	CTAG	2 cut(s)	162, 422
GluI	GCNGC	1 cut(s)	111
HapII	CCGG	2 cut(s)	96, 290
HgaI	GACGC	1 cut(s)	217
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	1 cut(s)	417
HindIII	AAGCTT	1 cut(s)	602
HinfI	GANTC	2 cut(s)	12, 427
HpaII	CCGG	2 cut(s)	96, 290
HphI	GGTGA	2 cut(s)	113, 374
Hpy166II	GTNNAC	1 cut(s)	118
Hpy188I	TCNGA	1 cut(s)	168
Hpy188III	TCNNGA	3 cut(s)	9, 314, 342
Hpy8I	GTNNAC	1 cut(s)	118
Hpy99I	CGWCG	1 cut(s)	233
HpyCH4III	ACNGT	4 cut(s)	24, 44, 122, 248
HpyCH4IV	ACGT	1 cut(s)	105
HpyCH4V	TGCA	6 cut(s)	118, 155, 284, 326, 386, 413
HpyF10VI	GCNNNNNNNGC	1 cut(s)	92
HpyF3I	CTNAG	1 cut(s)	442
HpySE526I	ACGT	1 cut(s)	105
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	1 cut(s)	417
Ksp22I	TGATCA	1 cut(s)	60
Kzo9I	GATC	2 cut(s)	60, 511
LguI	GCTCTTC	1 cut(s)	76
Lsp1109I	GCAGC	1 cut(s)	97
LweI	GCATC	2 cut(s)	162, 335
MaeI	CTAG	2 cut(s)	162, 422
MaeII	ACGT	1 cut(s)	105
MaeIII	GTNAC	2 cut(s)	74, 101
MalI	GATC	2 cut(s)	62, 513
MboI	GATC	2 cut(s)	60, 511
MboII	GAAGA	5 cut(s)	93, 181, 447, 476, 568
MhlI	GDGCHC	2 cut(s)	120, 441
MluCI	AATT	7 cut(s)	139, 146, 198, 251, 271, 449, 534
MlyI	GAGTC	2 cut(s)	6, 421
MnlI	CCTC	3 cut(s)	92, 215, 451
MseI	TTAA	4 cut(s)	132, 182, 204, 649
MspI	CCGG	2 cut(s)	96, 290
MspR9I	CCNGG	2 cut(s)	29, 351
MvaI	CCWGG	2 cut(s)	29, 351
MwoI	GCNNNNNNNGC	1 cut(s)	92
NdeII	GATC	2 cut(s)	60, 511
NlaIII	CATG	1 cut(s)	417
NmeAIII	GCCGAG	1 cut(s)	506
NmuCI	GTSAC	1 cut(s)	101
NspI	RCATGY	1 cut(s)	417
PciSI	GCTCTTC	1 cut(s)	76
PfoI	TCCNGGA	1 cut(s)	349
PkrI	GCNGC	1 cut(s)	112
PleI	GAGTC	2 cut(s)	6, 421
PpsI	GAGTC	2 cut(s)	6, 421
Psp6I	CCWGG	2 cut(s)	27, 349
PspGI	CCWGG	2 cut(s)	27, 349
SapI	GCTCTTC	1 cut(s)	76
SaqAI	TTAA	4 cut(s)	132, 182, 204, 649
SatI	GCNGC	1 cut(s)	111
Sau3AI	GATC	2 cut(s)	60, 511
SchI	GAGTC	2 cut(s)	6, 421
ScrFI	CCNGG	2 cut(s)	29, 351
SduI	GDGCHC	2 cut(s)	120, 441
SfaNI	GCATC	2 cut(s)	162, 335
SpeI	ACTAGT	1 cut(s)	161
Sse9I	AATT	7 cut(s)	139, 146, 198, 251, 271, 449, 534
SsiI	CCGC	1 cut(s)	113
SspMI	CTAG	2 cut(s)	162, 422
StyD4I	CCNGG	2 cut(s)	27, 349
TaaI	ACNGT	4 cut(s)	24, 44, 122, 248
TaiI	ACGT	1 cut(s)	108
TaqI	TCGA	1 cut(s)	10
TasI	AATT	7 cut(s)	139, 146, 198, 251, 271, 449, 534
Tru1I	TTAA	4 cut(s)	132, 182, 204, 649
Tru9I	TTAA	4 cut(s)	132, 182, 204, 649
TscAI	CASTG	1 cut(s)	465
TseFI	GTSAC	1 cut(s)	101
TseI	GCWGC	1 cut(s)	110
Tsp45I	GTSAC	1 cut(s)	101
TspDTI	ATGAA	3 cut(s)	480, 489, 558
TspGWI	ACGGA	2 cut(s)	93, 245
TspRI	CASTG	1 cut(s)	465
VneI	GTGCAC	1 cut(s)	116
XapI	RAATTY	3 cut(s)	139, 146, 251
XceI	RCATGY	1 cut(s)	417
XspI	CTAG	2 cut(s)	162, 422

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.