Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0000464

Physical Location & Seq

Genomic Coordinates Reverse (-)

15423084 .. 15425895

Gene Structure 2812 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g18470.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1065 bp

ATGATGTCTCGGCTGTGCCAACTGTTCCACGCCTACGAGGACGACGCCAATGTCAAGCTGGTCATTCTCAAAGGCAAAGGAAGAGCATTCTGCGCCGGCGGCGATGTGGTGGTGGTGGCTCGTCATCTTTTCGAAGGCAACTGGAGATTTGGAGCGCGACAAGGTAAGAGGGTGTATACCCTAGGCTACTTGATAGCAAAAAACACTACGCCCCAGGTTTCATTTCTCAATGGAATTGTCATGGGAGGTGGCGCCGGAGTTTCTATACACGGCAGGTTTCGTGTAGCAACTGAGAACTCGGTGTTTGCTTTGCCGGAAACAGCTCTGGGATTGTTTCCCGATTTGGGTGCTTCTTATTTTCTGTCAAGGCTTCCTGGATTCTTTGGAGAATATCTTGGTCTTACTGGTGCTAGATTGGACGGTCCTGAAATGCTCGCTTGTGGTTTAGCAACTCACTTTGTTCCCTCAGCCAAATTAGCCTCGTTAGAAGAAGCCCTAGTTGCTTCCACAAATATATCGAGCAGTGATCTTGTTGATAATATTTCAGCCATTATTAATGAATACTCACAGCAACCAGCTGTGAAAGAGAAGAGCGTTTGTCACAGAATAGATGTTATTGACAAGTGCTTTTCTAAGAGCACAGTGGAGGAAATTTTAAGTTCTCTTGAGAAGGAGCTTGCTACAACTGGTATACATGATCGTGATCACTGGTTGTCTTCATCGATTGCATCCCTGAAAAAGGCGTCACCGATAAGTTTGAAGATCACATTGAGATCGATTCGAGAAGGAAGGATGCAAGGAGTTGGCGAGTGCCTTGTTCGTGAGCAAAGAATGGTTTGCCATGTTGTGAGAGGGGAGATCAGTAAGGACTTTGGGGAGGGTTGTAGAGCGATATTGTTGGACAAGGATAAGAACCCGAAATGGGAGCCTTCTAAGTTGGAGCATATCACTGACGAGATGGTTGATCGCTACTTCTCTAAGTTGGATAGTGATGATGAAGAATTGGAAGAGTTGAAGCTTCCTGAAAGATCCAATTTGTATGGTTCTGCTATTGCCAAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

355

Amino Acids

38.95

Weight (kDa)

6.34

Isoelectric Point (pI)

34.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	1 - 325	1.4e-108	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	2 - 224	1.6e-24	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30650 AT2G30650 AT2G30660 AT2G30660 AT5G65940 AT5G65940 AT5G65940 AT5G65940
fragaria_vesca	FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g08010 FvH4_7g09160 FvH4_7g15030 FvH4_7g18470
malus_domestica	MD00G1098500.v1.1 MD02G1229900.v1.1 MD07G1169300.v1.1 MD07G1169400.v1.1
prunus_persica	Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G109100_v2.0.a1 Prupe.2G207200_v2.0.a1 Prupe.2G207400_v2.0.a1 Prupe.2G207600_v2.0.a1 Prupe.2G207700_v2.0.a1 Prupe.2G207800_v2.0.a1
pyrus_communis	pycom02g19850 pycom07g06680 pycom07g16310
rosa_chinensis	RchiOBHm_Chr1g0341301 RchiOBHm_Chr1g0345221 RchiOBHm_Chr1g0357091 RchiOBHm_Chr1g0357121 RchiOBHm_Chr1g0357151
rosa_laevigata	RLG00000027659 RLG00000028049 RLG00000028052 RLG00000029082 RLG00000029626
rosa_multiflora	Rmu_sc0000117.1_g000042 Rmu_sc0000166.1_g000050 Rmu_sc0004092.1_g000048 Rmu_sc0005967.1_g000013 Rmu_sc0006896.1_g000007 Rmu_sc0011699.1_g000007 Rmu_sc0013980.1_g000002 Rmu_sc0021527.1_g000001 Rmu_sc0024834.1_g000001 Rmu_ssc0000009.1_g000009
rosa_roxburghii	Rroxscaffold_4G00294310 Rroxscaffold_4G00299050 Rroxscaffold_4G00311940
rosa_rugosa	Rorug01G0157800.1 Rorug01G0254200 Rorug01G0254300.1 Rorug01G0296600.1 Rorug01G0296800 Rorug01G0296900 Rorug06G0065700
rosa_samantha	Rh1AG129700 Rh1AG162000 Rh1AG172400 Rh1AG268500 Rh1AG268600 Rh1AG305700 Rh1BG140400 Rh1BG236100 Rh1BG236200 Rh1BG269000 Rh1BG269100 Rh1CG123500 Rh1CG151300 Rh1CG160500 Rh1CG252100 Rh1CG252300 Rh1CG286900 Rh1DG172700 Rh1DG263300 Rh1DG263400 Rh1DG299000 Rh1DG299100
rosa_wichuraiana	Rw0G018900 Rw0G022950 Rw1G013330 Rw1G014380 Rw1G023680 Rw1G023700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	50
Acc36I	ACCTGC	1 cut(s)	264
AccB1I	GGYRCC	1 cut(s)	251
AccI	GTMKAC	2 cut(s)	176, 691
AccII	CGCG	1 cut(s)	157
AciI	CCGC	1 cut(s)	99
AclWI	GGATC	1 cut(s)	1023
AcsI	RAATTY	1 cut(s)	651
AcyI	GRCGYC	3 cut(s)	45, 252, 743
AfiI	CCNNNNNNNGG	3 cut(s)	344, 739, 922
AgsI	TTSAA	2 cut(s)	760, 1015
AjnI	CCWGG	2 cut(s)	213, 373
AluBI	AGCT	6 cut(s)	58, 323, 578, 676, 1018, 1060
AluI	AGCT	6 cut(s)	58, 323, 578, 676, 1018, 1060
Alw21I	GWGCWC	1 cut(s)	641
Alw26I	GTCTC	1 cut(s)	12
AlwI	GGATC	1 cut(s)	1023
ApoI	RAATTY	1 cut(s)	651
AseI	ATTAAT	1 cut(s)	555
AspA2I	CCTAGG	1 cut(s)	181
AspLEI	GCGC	3 cut(s)	95, 157, 254
AspS9I	GGNCC	1 cut(s)	422
AsuHPI	GGTGA	1 cut(s)	738
AsuII	TTCGAA	1 cut(s)	132
AvaII	GGWCC	1 cut(s)	422
AvrII	CCTAGG	1 cut(s)	181
BanI	GGYRCC	1 cut(s)	251
BbsI	GAAGAC	1 cut(s)	708
Bbv12I	GWGCWC	1 cut(s)	641
BbvCI	CCTCAGC	1 cut(s)	466
BccI	CCATC	1 cut(s)	952
BceAI	ACGGC	1 cut(s)	286
BcgI	CGANNNNNNTGC	2 cut(s)	329, 363
BciT130I	CCWGG	2 cut(s)	215, 375
BclI	TGATCA	1 cut(s)	703
BcoDI	GTCTC	1 cut(s)	12
BfaI	CTAG	3 cut(s)	182, 411, 497
BfoI	RGCGCY	1 cut(s)	255
BfuAI	ACCTGC	1 cut(s)	264
BisI	GCNGC	1 cut(s)	100
BlnI	CCTAGG	1 cut(s)	181
BlsI	GCNGC	1 cut(s)	101
Bme1390I	CCNGG	2 cut(s)	215, 375
Bme18I	GGWCC	1 cut(s)	422
BmgT120I	GGNCC	1 cut(s)	422
BmiI	GGNNCC	2 cut(s)	253, 927
BmrFI	CCNGG	2 cut(s)	215, 375
BmsI	GCATC	2 cut(s)	737, 783
BpiI	GAAGAC	1 cut(s)	708
BpmI	CTGGAG	1 cut(s)	163
Bpu10I	CCTNAGC	1 cut(s)	466
Bpu14I	TTCGAA	1 cut(s)	132
BpuEI	CTTGAG	1 cut(s)	686
Bsa29I	ATCGAT	2 cut(s)	722, 776
BsaBI	GATNNNNATC	1 cut(s)	702
BsaHI	GRCGYC	3 cut(s)	45, 252, 743
BsaJI	CCNNGG	2 cut(s)	181, 213
Bsc4I	CCNNNNNNNGG	3 cut(s)	344, 739, 922
Bse118I	RCCGGY	1 cut(s)	95
Bse1I	ACTGG	4 cut(s)	146, 409, 691, 713
Bse8I	GATNNNNATC	1 cut(s)	702
BseBI	CCWGG	2 cut(s)	215, 375
BseCI	ATCGAT	2 cut(s)	722, 776
BseDI	CCNNGG	2 cut(s)	181, 213
BseGI	GGATG	2 cut(s)	728, 798
BseJI	GATNNNNATC	1 cut(s)	702
BseLI	CCNNNNNNNGG	3 cut(s)	344, 739, 922
BseMII	CTCAG	2 cut(s)	282, 480
BseNI	ACTGG	4 cut(s)	146, 409, 691, 713
Bsh1236I	CGCG	1 cut(s)	157
BshNI	GGYRCC	1 cut(s)	251
BshVI	ATCGAT	2 cut(s)	722, 776
BsiHKAI	GWGCWC	1 cut(s)	641
BsiSI	CCGG	3 cut(s)	96, 255, 314
BslI	CCNNNNNNNGG	3 cut(s)	344, 739, 922
BsmAI	GTCTC	1 cut(s)	12
BsmI	GAATGC	1 cut(s)	86
Bsp119I	TTCGAA	1 cut(s)	132
Bsp1286I	GDGCHC	1 cut(s)	641
Bsp143I	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
BspACI	CCGC	1 cut(s)	99
BspCNI	CTCAG	2 cut(s)	283, 479
BspDI	ATCGAT	2 cut(s)	722, 776
BspFNI	CGCG	1 cut(s)	157
BspLI	GGNNCC	2 cut(s)	253, 927
BspMI	ACCTGC	1 cut(s)	264
BspPI	GGATC	1 cut(s)	1023
BspQI	GCTCTTC	2 cut(s)	76, 584
BspT104I	TTCGAA	1 cut(s)	132
BspT107I	GGYRCC	1 cut(s)	251
BsrFI	RCCGGY	1 cut(s)	95
BsrI	ACTGG	4 cut(s)	146, 409, 691, 713
BssAI	RCCGGY	1 cut(s)	95
BssECI	CCNNGG	2 cut(s)	181, 213
BssMI	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
BssNAI	GTATAC	2 cut(s)	177, 692
BssNI	GRCGYC	3 cut(s)	45, 252, 743
BssT1I	CCWWGG	1 cut(s)	181
Bst1107I	GTATAC	2 cut(s)	177, 692
Bst2UI	CCWGG	2 cut(s)	215, 375
Bst4CI	ACNGT	3 cut(s)	24, 422, 643
Bst6I	CTCTTC	3 cut(s)	76, 584, 1002
BstACI	GRCGYC	3 cut(s)	45, 252, 743
BstBI	TTCGAA	1 cut(s)	132
BstC8I	GCNNGC	3 cut(s)	97, 435, 678
BstDEI	CTNAG	5 cut(s)	291, 466, 633, 933, 978
BstENI	CCTNNNNNAGG	1 cut(s)	737
BstF5I	GGATG	2 cut(s)	728, 798
BstFNI	CGCG	1 cut(s)	157
BstH2I	RGCGCY	1 cut(s)	255
BstHHI	GCGC	3 cut(s)	95, 157, 254
BstKTI	GATC	8 cut(s)	529, 700, 706, 765, 776, 861, 967, 1031
BstMAI	GTCTC	1 cut(s)	12
BstMBI	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
BstMWI	GCNNNNNNNGC	4 cut(s)	92, 99, 476, 500
BstNI	CCWGG	2 cut(s)	215, 375
BstSCI	CCNGG	2 cut(s)	213, 373
BstUI	CGCG	1 cut(s)	157
BstV2I	GAAGAC	1 cut(s)	708
BstX2I	RGATCY	1 cut(s)	1028
BstYI	RGATCY	1 cut(s)	1028
BstZ17I	GTATAC	2 cut(s)	177, 692
Bsu15I	ATCGAT	2 cut(s)	722, 776
BsuTUI	ATCGAT	2 cut(s)	722, 776
BtgZI	GCGATG	1 cut(s)	117
BtsCI	GGATG	2 cut(s)	728, 798
BtsI	GCAGTG	1 cut(s)	529
BtsIMutI	CAGTG	4 cut(s)	529, 648, 706, 948
BveI	ACCTGC	1 cut(s)	264
Cac8I	GCNNGC	3 cut(s)	97, 435, 678
CfoI	GCGC	3 cut(s)	95, 157, 254
Cfr10I	RCCGGY	1 cut(s)	95
Cfr13I	GGNCC	1 cut(s)	422
ClaI	ATCGAT	2 cut(s)	722, 776
CseI	GACGC	2 cut(s)	53, 732
CviAII	CATG	3 cut(s)	241, 695, 842
DdeI	CTNAG	5 cut(s)	291, 466, 633, 933, 978
DinI	GGCGCC	1 cut(s)	253
DpnI	GATC	8 cut(s)	528, 699, 705, 764, 775, 860, 966, 1030
DpnII	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
DrdI	GACNNNNNNGTC	1 cut(s)	50
DseDI	GACNNNNNNGTC	1 cut(s)	50
Eam1104I	CTCTTC	3 cut(s)	76, 584, 1002
EarI	CTCTTC	3 cut(s)	76, 584, 1002
Eco130I	CCWWGG	1 cut(s)	181
Eco47I	GGWCC	1 cut(s)	422
EcoNI	CCTNNNNNAGG	1 cut(s)	737
EcoRII	CCWGG	2 cut(s)	213, 373
EcoT14I	CCWWGG	1 cut(s)	181
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	1 cut(s)	181
FaeI	CATG	3 cut(s)	244, 698, 845
FaiI	YATR	9 cut(s)	177, 242, 266, 515, 692, 696, 843, 945, 1041
FatI	CATG	3 cut(s)	240, 694, 841
FbaI	TGATCA	1 cut(s)	703
FblI	GTMKAC	2 cut(s)	176, 691
Fnu4HI	GCNGC	1 cut(s)	100
FokI	GGATG	2 cut(s)	715, 805
Fsp4HI	GCNGC	1 cut(s)	100
FspBI	CTAG	3 cut(s)	182, 411, 497
GlaI	GCGC	3 cut(s)	94, 156, 253
GluI	GCNGC	1 cut(s)	100
GsuI	CTGGAG	1 cut(s)	163
HaeII	RGCGCY	1 cut(s)	255
HapII	CCGG	3 cut(s)	96, 255, 314
HgaI	GACGC	2 cut(s)	53, 732
HhaI	GCGC	3 cut(s)	95, 157, 254
Hin1I	GRCGYC	3 cut(s)	45, 252, 743
Hin1II	CATG	3 cut(s)	244, 698, 845
Hin6I	GCGC	3 cut(s)	93, 155, 252
HinP1I	GCGC	3 cut(s)	93, 155, 252
HindIII	AAGCTT	2 cut(s)	1016, 1058
HinfI	GANTC	2 cut(s)	378, 778
HpaII	CCGG	3 cut(s)	96, 255, 314
HphI	GGTGA	1 cut(s)	738
Hpy166II	GTNNAC	2 cut(s)	177, 692
Hpy188III	TCNNGA	7 cut(s)	338, 425, 665, 701, 782, 821, 1022
Hpy8I	GTNNAC	2 cut(s)	177, 692
Hpy99I	CGWCG	1 cut(s)	47
HpyAV	CCTTC	5 cut(s)	128, 664, 779, 783, 939
HpyCH4III	ACNGT	3 cut(s)	24, 422, 643
HpyCH4V	TGCA	2 cut(s)	728, 796
HpyF10VI	GCNNNNNNNGC	4 cut(s)	92, 99, 476, 500
HpyF3I	CTNAG	5 cut(s)	291, 466, 633, 933, 978
Hsp92I	GRCGYC	3 cut(s)	45, 252, 743
Hsp92II	CATG	3 cut(s)	244, 698, 845
HspAI	GCGC	3 cut(s)	93, 155, 252
KasI	GGCGCC	1 cut(s)	251
KroI	GCCGGC	1 cut(s)	95
KroNI	GCCGGC	1 cut(s)	97
Ksp22I	TGATCA	1 cut(s)	703
Kzo9I	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
LguI	GCTCTTC	2 cut(s)	76, 584
LmnI	GCTCC	4 cut(s)	152, 673, 925, 940
LweI	GCATC	2 cut(s)	737, 783
MaeI	CTAG	3 cut(s)	182, 411, 497
MaeIII	GTNAC	2 cut(s)	599, 744
MalI	GATC	8 cut(s)	528, 699, 705, 764, 775, 860, 966, 1030
MboI	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
MboII	GAAGA	7 cut(s)	93, 500, 601, 708, 772, 1010, 1019
MflI	RGATCY	1 cut(s)	1028
MhlI	GDGCHC	1 cut(s)	641
MluCI	AATT	5 cut(s)	234, 473, 651, 1001, 1033
Mly113I	GGCGCC	1 cut(s)	252
MmeI	TCCRAC	3 cut(s)	879, 918, 963
MnlI	CCTC	8 cut(s)	31, 162, 239, 475, 490, 640, 845, 871
MreI	CGCCGGCG	1 cut(s)	95
MroNI	GCCGGC	1 cut(s)	95
MseI	TTAA	3 cut(s)	555, 656, 1063
MslI	CAYNNNNRTG	1 cut(s)	699
MspA1I	CMGCKG	1 cut(s)	578
MspI	CCGG	3 cut(s)	96, 255, 314
MspR9I	CCNGG	2 cut(s)	215, 375
Mva1269I	GAATGC	1 cut(s)	86
MvaI	CCWGG	2 cut(s)	215, 375
MvnI	CGCG	1 cut(s)	157
MwoI	GCNNNNNNNGC	4 cut(s)	92, 99, 476, 500
NaeI	GCCGGC	1 cut(s)	97
NarI	GGCGCC	1 cut(s)	252
NdeII	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
NgoMIV	GCCGGC	1 cut(s)	95
NlaIII	CATG	3 cut(s)	244, 698, 845
NlaIV	GGNNCC	2 cut(s)	253, 927
NmuCI	GTSAC	2 cut(s)	599, 744
NspV	TTCGAA	1 cut(s)	132
PciSI	GCTCTTC	2 cut(s)	76, 584
PctI	GAATGC	1 cut(s)	86
PdiI	GCCGGC	1 cut(s)	97
PfeI	GAWTC	2 cut(s)	378, 778
PfoI	TCCNGGA	1 cut(s)	373
PkrI	GCNGC	1 cut(s)	101
PluTI	GGCGCC	1 cut(s)	255
PshBI	ATTAAT	1 cut(s)	555
Psp6I	CCWGG	2 cut(s)	213, 373
PspGI	CCWGG	2 cut(s)	213, 373
PspN4I	GGNNCC	2 cut(s)	253, 927
PspPI	GGNCC	1 cut(s)	422
PsuI	RGATCY	1 cut(s)	1028
PvuII	CAGCTG	1 cut(s)	578
RseI	CAYNNNNRTG	1 cut(s)	699
SapI	GCTCTTC	2 cut(s)	76, 584
SaqAI	TTAA	3 cut(s)	555, 656, 1063
SatI	GCNGC	1 cut(s)	100
Sau3AI	GATC	8 cut(s)	526, 697, 703, 762, 773, 858, 964, 1028
Sau96I	GGNCC	1 cut(s)	422
ScrFI	CCNGG	2 cut(s)	215, 375
SduI	GDGCHC	1 cut(s)	641
SfaNI	GCATC	2 cut(s)	737, 783
SfoI	GGCGCC	1 cut(s)	253
SfuI	TTCGAA	1 cut(s)	132
SgrAI	CRCCGGYG	1 cut(s)	95
SinI	GGWCC	1 cut(s)	422
SmiMI	CAYNNNNRTG	1 cut(s)	699
SmlI	CTYRAG	1 cut(s)	665
SmoI	CTYRAG	1 cut(s)	665
Sse9I	AATT	5 cut(s)	234, 473, 651, 1001, 1033
SsiI	CCGC	1 cut(s)	99
SspDI	GGCGCC	1 cut(s)	251
SspI	AATATT	1 cut(s)	541
SspMI	CTAG	3 cut(s)	182, 411, 497
StyD4I	CCNGG	2 cut(s)	213, 373
StyI	CCWWGG	1 cut(s)	181
TaaI	ACNGT	3 cut(s)	24, 422, 643
TaqI	TCGA	5 cut(s)	132, 518, 722, 776, 781
TasI	AATT	5 cut(s)	234, 473, 651, 1001, 1033
TauI	GCSGC	1 cut(s)	102
TfiI	GAWTC	2 cut(s)	378, 778
Tru1I	TTAA	3 cut(s)	555, 656, 1063
Tru9I	TTAA	3 cut(s)	555, 656, 1063
TscAI	CASTG	4 cut(s)	529, 648, 713, 955
TseFI	GTSAC	2 cut(s)	599, 744
Tsp45I	GTSAC	2 cut(s)	599, 744
TspDTI	ATGAA	4 cut(s)	210, 573, 708, 1011
TspRI	CASTG	4 cut(s)	529, 648, 713, 955
VpaK11BI	GGWCC	1 cut(s)	422
VspI	ATTAAT	1 cut(s)	555
XagI	CCTNNNNNAGG	1 cut(s)	737
XapI	RAATTY	1 cut(s)	651
XcmI	CCANNNNNNNNNTGG	1 cut(s)	55
XmaJI	CCTAGG	1 cut(s)	181
XmiI	GTMKAC	2 cut(s)	176, 691
XspI	CTAG	3 cut(s)	182, 411, 497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.