Belongs to the enoyl-CoA hydratase isomerase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 467, 527 |
| AccII | CGCG | 1 cut(s) | 27 |
| AccIII | TCCGGA | 1 cut(s) | 457 |
| AciI | CCGC | 2 cut(s) | 215, 230 |
| AclWI | GGATC | 2 cut(s) | 672, 1113 |
| AcsI | RAATTY | 2 cut(s) | 140, 759 |
| AcuI | CTGAAG | 1 cut(s) | 875 |
| AfaI | GTAC | 1 cut(s) | 51 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 464, 778, 1018 |
| AgsI | TTSAA | 8 cut(s) | 103, 119, 588, 633, 653, 755, 824, 856 |
| AhlI | ACTAGT | 1 cut(s) | 590 |
| AjnI | CCWGG | 3 cut(s) | 68, 485, 493 |
| AluBI | AGCT | 7 cut(s) | 443, 656, 686, 986, 1039, 1108, 1150 |
| AluI | AGCT | 7 cut(s) | 443, 656, 686, 986, 1039, 1108, 1150 |
| Alw21I | GWGCWC | 2 cut(s) | 277, 1041 |
| Alw44I | GTGCAC | 1 cut(s) | 273 |
| AlwI | GGATC | 2 cut(s) | 672, 1113 |
| Aor13HI | TCCGGA | 1 cut(s) | 457 |
| ApaLI | GTGCAC | 1 cut(s) | 273 |
| ApeKI | GCWGC | 2 cut(s) | 611, 785 |
| ApoI | RAATTY | 2 cut(s) | 140, 759 |
| AseI | ATTAAT | 1 cut(s) | 249 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 931 |
| AsuHPI | GGTGA | 4 cut(s) | 193, 233, 701, 834 |
| BaeGI | GKGCMC | 1 cut(s) | 277 |
| BanI | GGYRCC | 2 cut(s) | 467, 527 |
| BanII | GRGCYC | 1 cut(s) | 1041 |
| Bbv12I | GWGCWC | 2 cut(s) | 277, 1041 |
| BbvI | GCAGC | 2 cut(s) | 623, 772 |
| BccI | CCATC | 3 cut(s) | 533, 709, 794 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 146, 180 |
| BciT130I | CCWGG | 3 cut(s) | 70, 487, 495 |
| BclI | TGATCA | 1 cut(s) | 1060 |
| BcuI | ACTAGT | 1 cut(s) | 590 |
| BfaI | CTAG | 4 cut(s) | 566, 591, 605, 741 |
| BisI | GCNGC | 3 cut(s) | 231, 612, 786 |
| BlsI | GCNGC | 3 cut(s) | 232, 613, 787 |
| Bme1390I | CCNGG | 3 cut(s) | 70, 487, 495 |
| BmiI | GGNNCC | 3 cut(s) | 469, 529, 1023 |
| BmrFI | CCNGG | 3 cut(s) | 70, 487, 495 |
| BmsI | GCATC | 3 cut(s) | 154, 688, 848 |
| BpuEI | CTTGAG | 2 cut(s) | 173, 794 |
| BsaJI | CCNNGG | 1 cut(s) | 388 |
| BsaWI | WCCGGW | 2 cut(s) | 457, 711 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 256, 286, 498, 528 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 464, 778, 1018 |
| Bse3DI | GCAATG | 2 cut(s) | 435, 1140 |
| BseAI | TCCGGA | 1 cut(s) | 457 |
| BseBI | CCWGG | 3 cut(s) | 70, 487, 495 |
| BseDI | CCNNGG | 1 cut(s) | 388 |
| BseGI | GGATG | 6 cut(s) | 169, 466, 544, 679, 720, 1087 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 464, 778, 1018 |
| BseMI | GCAATG | 2 cut(s) | 435, 1140 |
| BseMII | CTCAG | 1 cut(s) | 402 |
| BseRI | GAGGAG | 4 cut(s) | 173, 791, 794, 963 |
| BseSI | GKGCMC | 1 cut(s) | 277 |
| BseXI | GCAGC | 2 cut(s) | 623, 772 |
| Bsh1236I | CGCG | 1 cut(s) | 27 |
| BshNI | GGYRCC | 2 cut(s) | 467, 527 |
| BsiHKAI | GWGCWC | 2 cut(s) | 277, 1041 |
| BsiSI | CCGG | 2 cut(s) | 458, 712 |
| BslFI | GGGAC | 1 cut(s) | 462 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 464, 778, 1018 |
| BsmFI | GGGAC | 1 cut(s) | 462 |
| BsmI | GAATGC | 1 cut(s) | 109 |
| Bsp1286I | GDGCHC | 2 cut(s) | 277, 1041 |
| Bsp13I | TCCGGA | 1 cut(s) | 457 |
| Bsp143I | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| Bsp19I | CCATGG | 1 cut(s) | 388 |
| BspACI | CCGC | 2 cut(s) | 215, 230 |
| BspCNI | CTCAG | 1 cut(s) | 403 |
| BspEI | TCCGGA | 1 cut(s) | 457 |
| BspFNI | CGCG | 1 cut(s) | 27 |
| BspHI | TCATGA | 1 cut(s) | 241 |
| BspLI | GGNNCC | 3 cut(s) | 469, 529, 1023 |
| BspPI | GGATC | 2 cut(s) | 672, 1113 |
| BspQI | GCTCTTC | 1 cut(s) | 196 |
| BspT107I | GGYRCC | 2 cut(s) | 467, 527 |
| BsrDI | GCAATG | 2 cut(s) | 435, 1140 |
| BssECI | CCNNGG | 1 cut(s) | 388 |
| BssMI | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| BssT1I | CCWWGG | 1 cut(s) | 388 |
| Bst2UI | CCWGG | 3 cut(s) | 70, 487, 495 |
| Bst4CI | ACNGT | 3 cut(s) | 326, 421, 751 |
| Bst6I | CTCTTC | 2 cut(s) | 196, 1092 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 212 |
| BstC8I | GCNNGC | 1 cut(s) | 555 |
| BstDEI | CTNAG | 2 cut(s) | 411, 1074 |
| BstDSI | CCRYGG | 1 cut(s) | 388 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 776 |
| BstF5I | GGATG | 6 cut(s) | 169, 466, 544, 679, 720, 1087 |
| BstFNI | CGCG | 1 cut(s) | 27 |
| BstKTI | GATC | 5 cut(s) | 269, 667, 872, 1063, 1121 |
| BstMBI | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 212, 794 |
| BstNI | CCWGG | 3 cut(s) | 70, 487, 495 |
| BstSCI | CCNGG | 3 cut(s) | 68, 485, 493 |
| BstSLI | GKGCMC | 1 cut(s) | 277 |
| BstUI | CGCG | 1 cut(s) | 27 |
| BstV1I | GCAGC | 2 cut(s) | 623, 772 |
| BstX2I | RGATCY | 1 cut(s) | 1118 |
| BstYI | RGATCY | 1 cut(s) | 1118 |
| BtgI | CCRYGG | 1 cut(s) | 388 |
| BtgZI | GCGATG | 1 cut(s) | 42 |
| BtsCI | GGATG | 6 cut(s) | 169, 466, 544, 679, 720, 1087 |
| BtsIMutI | CAGTG | 1 cut(s) | 80 |
| Cac8I | GCNNGC | 1 cut(s) | 555 |
| CciI | TCATGA | 1 cut(s) | 241 |
| Csp6I | GTAC | 1 cut(s) | 50 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 559, 594 |
| CviAII | CATG | 4 cut(s) | 242, 361, 389, 938 |
| CviQI | GTAC | 1 cut(s) | 50 |
| DdeI | CTNAG | 2 cut(s) | 411, 1074 |
| DpnI | GATC | 5 cut(s) | 268, 666, 871, 1062, 1120 |
| DpnII | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| Eam1104I | CTCTTC | 2 cut(s) | 196, 1092 |
| EarI | CTCTTC | 2 cut(s) | 196, 1092 |
| Ecl136II | GAGCTC | 1 cut(s) | 1039 |
| Eco130I | CCWWGG | 1 cut(s) | 388 |
| Eco24I | GRGCYC | 1 cut(s) | 1041 |
| Eco53kI | GAGCTC | 1 cut(s) | 1039 |
| Eco57I | CTGAAG | 1 cut(s) | 875 |
| EcoICRI | GAGCTC | 1 cut(s) | 1039 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 776 |
| EcoRII | CCWGG | 3 cut(s) | 68, 485, 493 |
| EcoT14I | CCWWGG | 1 cut(s) | 388 |
| EcoT38I | GRGCYC | 1 cut(s) | 1041 |
| ErhI | CCWWGG | 1 cut(s) | 388 |
| FaeI | CATG | 4 cut(s) | 245, 364, 392, 941 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 873, 905 |
| FaqI | GGGAC | 1 cut(s) | 462 |
| FatI | CATG | 4 cut(s) | 241, 360, 388, 937 |
| FauI | CCCGC | 1 cut(s) | 208 |
| FbaI | TGATCA | 1 cut(s) | 1060 |
| Fnu4HI | GCNGC | 3 cut(s) | 231, 612, 786 |
| FokI | GGATG | 6 cut(s) | 176, 473, 551, 666, 727, 1094 |
| FriOI | GRGCYC | 1 cut(s) | 1041 |
| Fsp4HI | GCNGC | 3 cut(s) | 231, 612, 786 |
| FspBI | CTAG | 4 cut(s) | 566, 591, 605, 741 |
| GluI | GCNGC | 3 cut(s) | 231, 612, 786 |
| HapII | CCGG | 2 cut(s) | 458, 712 |
| Hin1II | CATG | 4 cut(s) | 245, 364, 392, 941 |
| HindIII | AAGCTT | 1 cut(s) | 1148 |
| HinfI | GANTC | 8 cut(s) | 35, 91, 132, 184, 396, 498, 629, 897 |
| HpaII | CCGG | 2 cut(s) | 458, 712 |
| HphI | GGTGA | 4 cut(s) | 193, 233, 701, 834 |
| Hpy166II | GTNNAC | 3 cut(s) | 275, 297, 329 |
| Hpy188I | TCNGA | 3 cut(s) | 287, 348, 971 |
| Hpy188III | TCNNGA | 5 cut(s) | 129, 242, 458, 741, 917 |
| Hpy8I | GTNNAC | 3 cut(s) | 275, 297, 329 |
| HpyAV | CCTTC | 6 cut(s) | 248, 594, 749, 875, 879, 1035 |
| HpyCH4III | ACNGT | 3 cut(s) | 326, 421, 751 |
| HpyCH4V | TGCA | 9 cut(s) | 152, 275, 407, 428, 621, 644, 788, 981, 1115 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 212, 794 |
| HpyF3I | CTNAG | 2 cut(s) | 411, 1074 |
| Hsp92II | CATG | 4 cut(s) | 245, 364, 392, 941 |
| Kpn2I | TCCGGA | 1 cut(s) | 457 |
| Ksp22I | TGATCA | 1 cut(s) | 1060 |
| Kzo9I | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| LguI | GCTCTTC | 1 cut(s) | 196 |
| LmnI | GCTCC | 3 cut(s) | 1021, 1036, 1113 |
| Lsp1109I | GCAGC | 2 cut(s) | 623, 772 |
| LweI | GCATC | 3 cut(s) | 154, 688, 848 |
| MaeI | CTAG | 4 cut(s) | 566, 591, 605, 741 |
| MaeIII | GTNAC | 1 cut(s) | 1132 |
| MalI | GATC | 5 cut(s) | 268, 666, 871, 1062, 1120 |
| MboI | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| MboII | GAAGA | 5 cut(s) | 213, 756, 868, 1100, 1109 |
| MfeI | CAATTG | 1 cut(s) | 98 |
| MflI | RGATCY | 1 cut(s) | 1118 |
| MhlI | GDGCHC | 2 cut(s) | 277, 1041 |
| MluCI | AATT | 8 cut(s) | 98, 140, 259, 354, 759, 819, 873, 1031 |
| MlyI | GAGTC | 3 cut(s) | 85, 126, 906 |
| MmeI | TCCRAC | 3 cut(s) | 975, 1059, 1146 |
| MroI | TCCGGA | 1 cut(s) | 457 |
| MroXI | GAANNNNTTC | 1 cut(s) | 931 |
| MseI | TTAA | 6 cut(s) | 111, 249, 302, 311, 866, 1103 |
| MspI | CCGG | 2 cut(s) | 458, 712 |
| MspR9I | CCNGG | 3 cut(s) | 70, 487, 495 |
| MunI | CAATTG | 1 cut(s) | 98 |
| Mva1269I | GAATGC | 1 cut(s) | 109 |
| MvaI | CCWGG | 3 cut(s) | 70, 487, 495 |
| MvnI | CGCG | 1 cut(s) | 27 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 212, 794 |
| NcoI | CCATGG | 1 cut(s) | 388 |
| NdeII | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| NlaIII | CATG | 4 cut(s) | 245, 364, 392, 941 |
| NlaIV | GGNNCC | 3 cut(s) | 469, 529, 1023 |
| NmeAIII | GCCGAG | 1 cut(s) | 73 |
| PagI | TCATGA | 1 cut(s) | 241 |
| PciSI | GCTCTTC | 1 cut(s) | 196 |
| PctI | GAATGC | 1 cut(s) | 109 |
| PdmI | GAANNNNTTC | 1 cut(s) | 931 |
| PfeI | GAWTC | 5 cut(s) | 35, 184, 396, 498, 629 |
| PfoI | TCCNGGA | 1 cut(s) | 493 |
| PkrI | GCNGC | 3 cut(s) | 232, 613, 787 |
| PleI | GAGTC | 3 cut(s) | 85, 126, 905 |
| PpsI | GAGTC | 3 cut(s) | 85, 126, 905 |
| PshBI | ATTAAT | 1 cut(s) | 249 |
| Psp124BI | GAGCTC | 1 cut(s) | 1041 |
| Psp6I | CCWGG | 3 cut(s) | 68, 485, 493 |
| PspGI | CCWGG | 3 cut(s) | 68, 485, 493 |
| PspN4I | GGNNCC | 3 cut(s) | 469, 529, 1023 |
| PsuI | RGATCY | 1 cut(s) | 1118 |
| RsaI | GTAC | 1 cut(s) | 51 |
| RsaNI | GTAC | 1 cut(s) | 50 |
| SacI | GAGCTC | 1 cut(s) | 1041 |
| SapI | GCTCTTC | 1 cut(s) | 196 |
| SaqAI | TTAA | 6 cut(s) | 111, 249, 302, 311, 866, 1103 |
| SatI | GCNGC | 3 cut(s) | 231, 612, 786 |
| Sau3AI | GATC | 5 cut(s) | 266, 664, 869, 1060, 1118 |
| SchI | GAGTC | 3 cut(s) | 85, 126, 906 |
| ScrFI | CCNGG | 3 cut(s) | 70, 487, 495 |
| SduI | GDGCHC | 2 cut(s) | 277, 1041 |
| SfaNI | GCATC | 3 cut(s) | 154, 688, 848 |
| SmlI | CTYRAG | 3 cut(s) | 16, 188, 773 |
| SmoI | CTYRAG | 3 cut(s) | 16, 188, 773 |
| SpeI | ACTAGT | 1 cut(s) | 590 |
| Sse9I | AATT | 8 cut(s) | 98, 140, 259, 354, 759, 819, 873, 1031 |
| SsiI | CCGC | 2 cut(s) | 215, 230 |
| SspMI | CTAG | 4 cut(s) | 566, 591, 605, 741 |
| SstI | GAGCTC | 1 cut(s) | 1041 |
| StyD4I | CCNGG | 3 cut(s) | 68, 485, 493 |
| StyI | CCWWGG | 1 cut(s) | 388 |
| TaaI | ACNGT | 3 cut(s) | 326, 421, 751 |
| TaqI | TCGA | 1 cut(s) | 130 |
| TaqII | GACCGA | 1 cut(s) | 890 |
| TasI | AATT | 8 cut(s) | 98, 140, 259, 354, 759, 819, 873, 1031 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 5 cut(s) | 35, 184, 396, 498, 629 |
| Tru1I | TTAA | 6 cut(s) | 111, 249, 302, 311, 866, 1103 |
| Tru9I | TTAA | 6 cut(s) | 111, 249, 302, 311, 866, 1103 |
| TscAI | CASTG | 1 cut(s) | 87 |
| TseI | GCWGC | 2 cut(s) | 611, 785 |
| TspDTI | ATGAA | 3 cut(s) | 230, 267, 1101 |
| TspGWI | ACGGA | 2 cut(s) | 373, 389 |
| TspRI | CASTG | 1 cut(s) | 87 |
| VneI | GTGCAC | 1 cut(s) | 273 |
| VspI | ATTAAT | 1 cut(s) | 249 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 776 |
| XapI | RAATTY | 2 cut(s) | 140, 759 |
| XbaI | TCTAGA | 1 cut(s) | 740 |
| XmnI | GAANNNNTTC | 1 cut(s) | 931 |
| XspI | CTAG | 4 cut(s) | 566, 591, 605, 741 |