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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 635 |
| AccBSI | CCGCTC | 2 cut(s) | 193, 365 |
| AccI | GTMKAC | 1 cut(s) | 213 |
| AciI | CCGC | 6 cut(s) | 13, 193, 365, 504, 633, 650 |
| AcsI | RAATTY | 1 cut(s) | 654 |
| AcuI | CTGAAG | 1 cut(s) | 122 |
| AcyI | GRCGYC | 1 cut(s) | 636 |
| AfaI | GTAC | 3 cut(s) | 11, 71, 694 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 248, 677 |
| AjnI | CCWGG | 1 cut(s) | 624 |
| AluBI | AGCT | 1 cut(s) | 647 |
| AluI | AGCT | 1 cut(s) | 647 |
| Alw21I | GWGCWC | 1 cut(s) | 33 |
| AoxI | GGCC | 1 cut(s) | 455 |
| ApeKI | GCWGC | 1 cut(s) | 647 |
| ApoI | RAATTY | 1 cut(s) | 654 |
| AspLEI | GCGC | 1 cut(s) | 638 |
| AsuHPI | GGTGA | 3 cut(s) | 51, 265, 352 |
| AxyI | CCTNAGG | 1 cut(s) | 288 |
| BanI | GGYRCC | 1 cut(s) | 635 |
| BbsI | GAAGAC | 1 cut(s) | 44 |
| Bbv12I | GWGCWC | 1 cut(s) | 33 |
| BbvI | GCAGC | 1 cut(s) | 634 |
| BccI | CCATC | 2 cut(s) | 139, 457 |
| BciT130I | CCWGG | 1 cut(s) | 626 |
| BfaI | CTAG | 1 cut(s) | 197 |
| BfoI | RGCGCY | 1 cut(s) | 639 |
| BisI | GCNGC | 4 cut(s) | 194, 366, 634, 648 |
| BlsI | GCNGC | 4 cut(s) | 195, 367, 635, 649 |
| Bme1390I | CCNGG | 1 cut(s) | 626 |
| BmiI | GGNNCC | 1 cut(s) | 637 |
| BmrFI | CCNGG | 1 cut(s) | 626 |
| BmsI | GCATC | 2 cut(s) | 343, 431 |
| BpiI | GAAGAC | 1 cut(s) | 44 |
| Bpu10I | CCTNAGC | 1 cut(s) | 459 |
| BsaHI | GRCGYC | 1 cut(s) | 636 |
| BsaJI | CCNNGG | 2 cut(s) | 395, 614 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 248, 677 |
| Bse21I | CCTNAGG | 1 cut(s) | 288 |
| Bse3DI | GCAATG | 1 cut(s) | 22 |
| BseBI | CCWGG | 1 cut(s) | 626 |
| BseDI | CCNNGG | 2 cut(s) | 395, 614 |
| BseGI | GGATG | 1 cut(s) | 406 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 248, 677 |
| BseMI | GCAATG | 1 cut(s) | 22 |
| BseMII | CTCAG | 1 cut(s) | 279 |
| BseRI | GAGGAG | 1 cut(s) | 20 |
| BseXI | GCAGC | 1 cut(s) | 634 |
| BshFI | GGCC | 1 cut(s) | 457 |
| BshNI | GGYRCC | 1 cut(s) | 635 |
| BsiHKAI | GWGCWC | 1 cut(s) | 33 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 248, 677 |
| BsnI | GGCC | 1 cut(s) | 457 |
| Bsp1286I | GDGCHC | 1 cut(s) | 33 |
| BspACI | CCGC | 6 cut(s) | 13, 193, 365, 504, 633, 650 |
| BspANI | GGCC | 1 cut(s) | 457 |
| BspCNI | CTCAG | 1 cut(s) | 280 |
| BspHI | TCATGA | 1 cut(s) | 75 |
| BspLI | GGNNCC | 1 cut(s) | 637 |
| BspT107I | GGYRCC | 1 cut(s) | 635 |
| BsrBI | CCGCTC | 2 cut(s) | 193, 365 |
| BsrDI | GCAATG | 1 cut(s) | 22 |
| BssECI | CCNNGG | 2 cut(s) | 395, 614 |
| BssNI | GRCGYC | 1 cut(s) | 636 |
| Bst2UI | CCWGG | 1 cut(s) | 626 |
| Bst4CI | ACNGT | 3 cut(s) | 116, 579, 615 |
| Bst6I | CTCTTC | 1 cut(s) | 528 |
| BstACI | GRCGYC | 1 cut(s) | 636 |
| BstC8I | GCNNGC | 2 cut(s) | 239, 631 |
| BstDEI | CTNAG | 2 cut(s) | 288, 459 |
| BstDSI | CCRYGG | 1 cut(s) | 614 |
| BstF5I | GGATG | 1 cut(s) | 406 |
| BstH2I | RGCGCY | 1 cut(s) | 639 |
| BstHHI | GCGC | 1 cut(s) | 638 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 362, 635, 644 |
| BstNI | CCWGG | 1 cut(s) | 626 |
| BstSCI | CCNGG | 1 cut(s) | 624 |
| BstV1I | GCAGC | 1 cut(s) | 634 |
| BstV2I | GAAGAC | 1 cut(s) | 44 |
| Bsu36I | CCTNAGG | 1 cut(s) | 288 |
| BsuRI | GGCC | 1 cut(s) | 457 |
| BtgI | CCRYGG | 1 cut(s) | 614 |
| BtsCI | GGATG | 1 cut(s) | 406 |
| Cac8I | GCNNGC | 2 cut(s) | 239, 631 |
| CciI | TCATGA | 1 cut(s) | 75 |
| CfoI | GCGC | 1 cut(s) | 638 |
| Csp6I | GTAC | 3 cut(s) | 10, 70, 693 |
| CviAII | CATG | 5 cut(s) | 26, 76, 92, 181, 690 |
| CviJI | RGCY | 7 cut(s) | 100, 177, 196, 286, 368, 457, 647 |
| CviKI_1 | RGCY | 7 cut(s) | 100, 177, 196, 286, 368, 457, 647 |
| CviQI | GTAC | 3 cut(s) | 10, 70, 693 |
| DdeI | CTNAG | 2 cut(s) | 288, 459 |
| DinI | GGCGCC | 1 cut(s) | 637 |
| DraI | TTTAAA | 1 cut(s) | 319 |
| Eam1104I | CTCTTC | 1 cut(s) | 528 |
| EarI | CTCTTC | 1 cut(s) | 528 |
| Eco147I | AGGCCT | 1 cut(s) | 457 |
| Eco57I | CTGAAG | 1 cut(s) | 122 |
| Eco81I | CCTNAGG | 1 cut(s) | 288 |
| EcoRII | CCWGG | 1 cut(s) | 624 |
| EcoT22I | ATGCAT | 1 cut(s) | 358 |
| EgeI | GGCGCC | 1 cut(s) | 637 |
| EheI | GGCGCC | 1 cut(s) | 637 |
| FaeI | CATG | 5 cut(s) | 29, 79, 95, 184, 693 |
| FatI | CATG | 5 cut(s) | 25, 75, 91, 180, 689 |
| FblI | GTMKAC | 1 cut(s) | 213 |
| Fnu4HI | GCNGC | 4 cut(s) | 194, 366, 634, 648 |
| FokI | GGATG | 1 cut(s) | 413 |
| Fsp4HI | GCNGC | 4 cut(s) | 194, 366, 634, 648 |
| FspBI | CTAG | 1 cut(s) | 197 |
| GlaI | GCGC | 1 cut(s) | 637 |
| GluI | GCNGC | 4 cut(s) | 194, 366, 634, 648 |
| HaeII | RGCGCY | 1 cut(s) | 639 |
| HaeIII | GGCC | 1 cut(s) | 457 |
| HhaI | GCGC | 1 cut(s) | 638 |
| Hin1I | GRCGYC | 1 cut(s) | 636 |
| Hin1II | CATG | 5 cut(s) | 29, 79, 95, 184, 693 |
| Hin6I | GCGC | 1 cut(s) | 636 |
| HinP1I | GCGC | 1 cut(s) | 636 |
| HincII | GTYRAC | 2 cut(s) | 127, 214 |
| HindII | GTYRAC | 2 cut(s) | 127, 214 |
| HinfI | GANTC | 1 cut(s) | 52 |
| HphI | GGTGA | 3 cut(s) | 51, 265, 352 |
| Hpy166II | GTNNAC | 2 cut(s) | 127, 214 |
| Hpy188I | TCNGA | 1 cut(s) | 415 |
| Hpy188III | TCNNGA | 2 cut(s) | 76, 374 |
| Hpy8I | GTNNAC | 2 cut(s) | 127, 214 |
| Hpy99I | CGWCG | 1 cut(s) | 218 |
| HpyAV | CCTTC | 3 cut(s) | 242, 461, 551 |
| HpyCH4III | ACNGT | 3 cut(s) | 116, 579, 615 |
| HpyCH4V | TGCA | 1 cut(s) | 356 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 362, 635, 644 |
| HpyF3I | CTNAG | 2 cut(s) | 288, 459 |
| Hsp92I | GRCGYC | 1 cut(s) | 636 |
| Hsp92II | CATG | 5 cut(s) | 29, 79, 95, 184, 693 |
| HspAI | GCGC | 1 cut(s) | 636 |
| KasI | GGCGCC | 1 cut(s) | 635 |
| LmnI | GCTCC | 1 cut(s) | 190 |
| LpnPI | CCDG | 8 cut(s) | 52, 300, 478, 503, 535, 611, 638, 674 |
| Lsp1109I | GCAGC | 1 cut(s) | 634 |
| LweI | GCATC | 2 cut(s) | 343, 431 |
| MaeI | CTAG | 1 cut(s) | 197 |
| MaeIII | GTNAC | 3 cut(s) | 293, 331, 374 |
| MbiI | CCGCTC | 2 cut(s) | 193, 365 |
| MboII | GAAGA | 4 cut(s) | 49, 115, 118, 545 |
| MhlI | GDGCHC | 1 cut(s) | 33 |
| MluCI | AATT | 2 cut(s) | 433, 654 |
| Mly113I | GGCGCC | 1 cut(s) | 636 |
| MnlI | CCTC | 6 cut(s) | 89, 283, 390, 456, 464, 529 |
| Mph1103I | ATGCAT | 1 cut(s) | 358 |
| MseI | TTAA | 4 cut(s) | 138, 260, 318, 525 |
| MspR9I | CCNGG | 1 cut(s) | 626 |
| MvaI | CCWGG | 1 cut(s) | 626 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 362, 635, 644 |
| NarI | GGCGCC | 1 cut(s) | 636 |
| NlaIII | CATG | 5 cut(s) | 29, 79, 95, 184, 693 |
| NlaIV | GGNNCC | 1 cut(s) | 637 |
| NmuCI | GTSAC | 1 cut(s) | 374 |
| NsiI | ATGCAT | 1 cut(s) | 358 |
| PagI | TCATGA | 1 cut(s) | 75 |
| PceI | AGGCCT | 1 cut(s) | 457 |
| PfeI | GAWTC | 1 cut(s) | 52 |
| PkrI | GCNGC | 4 cut(s) | 195, 367, 635, 649 |
| PluTI | GGCGCC | 1 cut(s) | 639 |
| Psp6I | CCWGG | 1 cut(s) | 624 |
| PspGI | CCWGG | 1 cut(s) | 624 |
| PspN4I | GGNNCC | 1 cut(s) | 637 |
| RsaI | GTAC | 3 cut(s) | 11, 71, 694 |
| RsaNI | GTAC | 3 cut(s) | 10, 70, 693 |
| SalI | GTCGAC | 1 cut(s) | 212 |
| SaqAI | TTAA | 4 cut(s) | 138, 260, 318, 525 |
| SatI | GCNGC | 4 cut(s) | 194, 366, 634, 648 |
| ScrFI | CCNGG | 1 cut(s) | 626 |
| SduI | GDGCHC | 1 cut(s) | 33 |
| SetI | ASST | 6 cut(s) | 64, 126, 342, 448, 627, 649 |
| SfaNI | GCATC | 2 cut(s) | 343, 431 |
| SfoI | GGCGCC | 1 cut(s) | 637 |
| Sse9I | AATT | 2 cut(s) | 433, 654 |
| SseBI | AGGCCT | 1 cut(s) | 457 |
| SsiI | CCGC | 6 cut(s) | 13, 193, 365, 504, 633, 650 |
| SspDI | GGCGCC | 1 cut(s) | 635 |
| SspMI | CTAG | 1 cut(s) | 197 |
| StuI | AGGCCT | 1 cut(s) | 457 |
| StyD4I | CCNGG | 1 cut(s) | 624 |
| TaaI | ACNGT | 3 cut(s) | 116, 579, 615 |
| TaqI | TCGA | 2 cut(s) | 213, 574 |
| TaqII | GACCGA | 1 cut(s) | 409 |
| TasI | AATT | 2 cut(s) | 433, 654 |
| TatI | WGTACW | 1 cut(s) | 692 |
| TauI | GCSGC | 3 cut(s) | 196, 368, 636 |
| TfiI | GAWTC | 1 cut(s) | 52 |
| Tru1I | TTAA | 4 cut(s) | 138, 260, 318, 525 |
| Tru9I | TTAA | 4 cut(s) | 138, 260, 318, 525 |
| TseFI | GTSAC | 1 cut(s) | 374 |
| TseI | GCWGC | 1 cut(s) | 647 |
| Tsp45I | GTSAC | 1 cut(s) | 374 |
| TspDTI | ATGAA | 7 cut(s) | 33, 64, 65, 92, 169, 289, 528 |
| XapI | RAATTY | 1 cut(s) | 654 |
| XmiI | GTMKAC | 1 cut(s) | 213 |
| XspI | CTAG | 1 cut(s) | 197 |
| Zsp2I | ATGCAT | 1 cut(s) | 358 |