ubiquitinyl hydrolase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 466 |
| AciI | CCGC | 2 cut(s) | 735, 784 |
| AclWI | GGATC | 3 cut(s) | 540, 781, 794 |
| AcsI | RAATTY | 2 cut(s) | 986, 1093 |
| AcuI | CTGAAG | 1 cut(s) | 821 |
| AfaI | GTAC | 3 cut(s) | 80, 332, 948 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 70, 71, 75, 466 |
| AflIII | ACRYGT | 1 cut(s) | 949 |
| AgsI | TTSAA | 8 cut(s) | 253, 353, 686, 855, 917, 985, 1058, 1238 |
| AjnI | CCWGG | 3 cut(s) | 53, 69, 554 |
| AluBI | AGCT | 8 cut(s) | 60, 85, 203, 217, 295, 366, 894, 944 |
| AluI | AGCT | 8 cut(s) | 60, 85, 203, 217, 295, 366, 894, 944 |
| Alw21I | GWGCWC | 2 cut(s) | 219, 886 |
| Alw44I | GTGCAC | 1 cut(s) | 882 |
| AlwI | GGATC | 3 cut(s) | 540, 781, 794 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 975 |
| Ama87I | CYCGRG | 2 cut(s) | 258, 391 |
| AoxI | GGCC | 3 cut(s) | 222, 457, 1138 |
| ApaI | GGGCCC | 1 cut(s) | 1142 |
| ApaLI | GTGCAC | 1 cut(s) | 882 |
| ApeKI | GCWGC | 3 cut(s) | 292, 366, 1211 |
| ApoI | RAATTY | 2 cut(s) | 986, 1093 |
| AspS9I | GGNCC | 5 cut(s) | 223, 457, 1138, 1139, 1162 |
| AsuC2I | CCSGG | 1 cut(s) | 845 |
| AsuHPI | GGTGA | 3 cut(s) | 489, 819, 1093 |
| AsuII | TTCGAA | 1 cut(s) | 145 |
| AvaI | CYCGRG | 2 cut(s) | 258, 391 |
| AvaII | GGWCC | 1 cut(s) | 1162 |
| AxyI | CCTNAGG | 1 cut(s) | 864 |
| BaeGI | GKGCMC | 2 cut(s) | 886, 1142 |
| BamHI | GGATCC | 1 cut(s) | 786 |
| BanII | GRGCYC | 2 cut(s) | 219, 1142 |
| Bbv12I | GWGCWC | 2 cut(s) | 219, 886 |
| BbvI | GCAGC | 3 cut(s) | 304, 353, 1198 |
| BccI | CCATC | 5 cut(s) | 89, 351, 390, 721, 1201 |
| BciT130I | CCWGG | 3 cut(s) | 55, 71, 556 |
| BciVI | GTATCC | 1 cut(s) | 724 |
| BcnI | CCSGG | 1 cut(s) | 845 |
| BfaI | CTAG | 3 cut(s) | 446, 876, 1260 |
| BfuI | GTATCC | 1 cut(s) | 724 |
| BisI | GCNGC | 3 cut(s) | 293, 367, 1212 |
| BlsI | GCNGC | 3 cut(s) | 294, 368, 1213 |
| Bme1390I | CCNGG | 4 cut(s) | 55, 71, 556, 845 |
| Bme18I | GGWCC | 1 cut(s) | 1162 |
| BmeT110I | CYCGRG | 2 cut(s) | 258, 391 |
| BmgT120I | GGNCC | 5 cut(s) | 223, 457, 1138, 1139, 1162 |
| BmiI | GGNNCC | 3 cut(s) | 788, 1140, 1164 |
| BmrFI | CCNGG | 4 cut(s) | 55, 71, 556, 845 |
| BmsI | GCATC | 2 cut(s) | 1213, 1273 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 49, 81 |
| BpmI | CTGGAG | 1 cut(s) | 76 |
| Bpu14I | TTCGAA | 1 cut(s) | 145 |
| BpuEI | CTTGAG | 3 cut(s) | 203, 293, 548 |
| BpuMI | CCSGG | 1 cut(s) | 845 |
| Bsa29I | ATCGAT | 1 cut(s) | 1149 |
| BsaJI | CCNNGG | 5 cut(s) | 69, 70, 611, 1018, 1062 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 29, 59, 123, 150, 153, 180 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 70, 71, 75, 466 |
| Bse118I | RCCGGY | 1 cut(s) | 1214 |
| Bse21I | CCTNAGG | 1 cut(s) | 864 |
| Bse3DI | GCAATG | 1 cut(s) | 376 |
| BseBI | CCWGG | 3 cut(s) | 55, 71, 556 |
| BseCI | ATCGAT | 1 cut(s) | 1149 |
| BseDI | CCNNGG | 5 cut(s) | 69, 70, 611, 1018, 1062 |
| BseGI | GGATG | 4 cut(s) | 682, 844, 853, 1288 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 70, 71, 75, 466 |
| BseMI | GCAATG | 1 cut(s) | 376 |
| BseMII | CTCAG | 3 cut(s) | 131, 878, 1015 |
| BseRI | GAGGAG | 2 cut(s) | 53, 144 |
| BseSI | GKGCMC | 2 cut(s) | 886, 1142 |
| BseXI | GCAGC | 3 cut(s) | 304, 353, 1198 |
| BseYI | CCCAGC | 1 cut(s) | 920 |
| BsgI | GTGCAG | 2 cut(s) | 212, 1107 |
| BshFI | GGCC | 3 cut(s) | 224, 459, 1140 |
| BshVI | ATCGAT | 1 cut(s) | 1149 |
| BsiHKAI | GWGCWC | 2 cut(s) | 219, 886 |
| BsiHKCI | CYCGRG | 2 cut(s) | 258, 391 |
| BsiSI | CCGG | 3 cut(s) | 450, 845, 1215 |
| BslFI | GGGAC | 1 cut(s) | 827 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 70, 71, 75, 466 |
| BsmFI | GGGAC | 1 cut(s) | 827 |
| BsmI | GAATGC | 3 cut(s) | 595, 661, 814 |
| BsnI | GGCC | 3 cut(s) | 224, 459, 1140 |
| BsoBI | CYCGRG | 2 cut(s) | 258, 391 |
| Bsp119I | TTCGAA | 1 cut(s) | 145 |
| Bsp120I | GGGCCC | 1 cut(s) | 1138 |
| Bsp1286I | GDGCHC | 3 cut(s) | 219, 886, 1142 |
| Bsp1407I | TGTACA | 1 cut(s) | 946 |
| Bsp143I | GATC | 3 cut(s) | 532, 679, 786 |
| Bsp19I | CCATGG | 1 cut(s) | 1018 |
| BspACI | CCGC | 2 cut(s) | 735, 784 |
| BspANI | GGCC | 3 cut(s) | 224, 459, 1140 |
| BspCNI | CTCAG | 3 cut(s) | 130, 877, 1014 |
| BspDI | ATCGAT | 1 cut(s) | 1149 |
| BspHI | TCATGA | 1 cut(s) | 1219 |
| BspLI | GGNNCC | 3 cut(s) | 788, 1140, 1164 |
| BspPI | GGATC | 3 cut(s) | 540, 781, 794 |
| BspT104I | TTCGAA | 1 cut(s) | 145 |
| BsrDI | GCAATG | 1 cut(s) | 376 |
| BsrFI | RCCGGY | 1 cut(s) | 1214 |
| BsrGI | TGTACA | 1 cut(s) | 946 |
| BssAI | RCCGGY | 1 cut(s) | 1214 |
| BssECI | CCNNGG | 5 cut(s) | 69, 70, 611, 1018, 1062 |
| BssMI | GATC | 3 cut(s) | 532, 679, 786 |
| BssT1I | CCWWGG | 3 cut(s) | 611, 1018, 1062 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 71, 556 |
| Bst4CI | ACNGT | 5 cut(s) | 431, 901, 971, 977, 1114 |
| Bst6I | CTCTTC | 1 cut(s) | 573 |
| BstAUI | TGTACA | 1 cut(s) | 946 |
| BstBI | TTCGAA | 1 cut(s) | 145 |
| BstC8I | GCNNGC | 1 cut(s) | 297 |
| BstDEI | CTNAG | 6 cut(s) | 117, 644, 723, 864, 1001, 1089 |
| BstDSI | CCRYGG | 1 cut(s) | 1018 |
| BstF5I | GGATG | 4 cut(s) | 682, 844, 853, 1288 |
| BstKTI | GATC | 3 cut(s) | 535, 682, 789 |
| BstMBI | GATC | 3 cut(s) | 532, 679, 786 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 209 |
| BstNI | CCWGG | 3 cut(s) | 55, 71, 556 |
| BstNSI | RCATGY | 2 cut(s) | 953, 1185 |
| BstSCI | CCNGG | 4 cut(s) | 53, 69, 554, 843 |
| BstSLI | GKGCMC | 2 cut(s) | 886, 1142 |
| BstV1I | GCAGC | 3 cut(s) | 304, 353, 1198 |
| BstX2I | RGATCY | 1 cut(s) | 786 |
| BstYI | RGATCY | 1 cut(s) | 786 |
| Bsu15I | ATCGAT | 1 cut(s) | 1149 |
| Bsu36I | CCTNAGG | 1 cut(s) | 864 |
| BsuI | GTATCC | 1 cut(s) | 724 |
| BsuRI | GGCC | 3 cut(s) | 224, 459, 1140 |
| BsuTUI | ATCGAT | 1 cut(s) | 1149 |
| BtgI | CCRYGG | 1 cut(s) | 1018 |
| BtsCI | GGATG | 4 cut(s) | 682, 844, 853, 1288 |
| BtsIMutI | CAGTG | 4 cut(s) | 125, 906, 964, 976 |
| Cac8I | GCNNGC | 1 cut(s) | 297 |
| CaiI | CAGNNNCTG | 1 cut(s) | 975 |
| CciI | TCATGA | 1 cut(s) | 1219 |
| Cfr10I | RCCGGY | 1 cut(s) | 1214 |
| Cfr13I | GGNCC | 5 cut(s) | 223, 457, 1138, 1139, 1162 |
| ClaI | ATCGAT | 1 cut(s) | 1149 |
| CsiI | ACCWGGT | 1 cut(s) | 554 |
| Csp6I | GTAC | 3 cut(s) | 79, 331, 947 |
| CviAII | CATG | 6 cut(s) | 649, 702, 950, 1019, 1182, 1220 |
| CviQI | GTAC | 3 cut(s) | 79, 331, 947 |
| DdeI | CTNAG | 6 cut(s) | 117, 644, 723, 864, 1001, 1089 |
| DpnI | GATC | 3 cut(s) | 534, 681, 788 |
| DpnII | GATC | 3 cut(s) | 532, 679, 786 |
| DraI | TTTAAA | 2 cut(s) | 381, 1098 |
| Eam1104I | CTCTTC | 1 cut(s) | 573 |
| EarI | CTCTTC | 1 cut(s) | 573 |
| EciI | GGCGGA | 1 cut(s) | 799 |
| Ecl136II | GAGCTC | 1 cut(s) | 217 |
| Eco130I | CCWWGG | 3 cut(s) | 611, 1018, 1062 |
| Eco24I | GRGCYC | 2 cut(s) | 219, 1142 |
| Eco32I | GATATC | 1 cut(s) | 1147 |
| Eco47I | GGWCC | 1 cut(s) | 1162 |
| Eco53kI | GAGCTC | 1 cut(s) | 217 |
| Eco57I | CTGAAG | 1 cut(s) | 821 |
| Eco81I | CCTNAGG | 1 cut(s) | 864 |
| Eco88I | CYCGRG | 2 cut(s) | 258, 391 |
| EcoICRI | GAGCTC | 1 cut(s) | 217 |
| EcoO109I | RGGNCCY | 4 cut(s) | 223, 1138, 1139, 1162 |
| EcoRII | CCWGG | 3 cut(s) | 53, 69, 554 |
| EcoRV | GATATC | 1 cut(s) | 1147 |
| EcoT14I | CCWWGG | 3 cut(s) | 611, 1018, 1062 |
| EcoT38I | GRGCYC | 2 cut(s) | 219, 1142 |
| ErhI | CCWWGG | 3 cut(s) | 611, 1018, 1062 |
| FaeI | CATG | 6 cut(s) | 652, 705, 953, 1022, 1185, 1223 |
| FaqI | GGGAC | 1 cut(s) | 827 |
| FatI | CATG | 6 cut(s) | 648, 701, 949, 1018, 1181, 1219 |
| Fnu4HI | GCNGC | 3 cut(s) | 293, 367, 1212 |
| FokI | GGATG | 3 cut(s) | 689, 851, 860 |
| FriOI | GRGCYC | 2 cut(s) | 219, 1142 |
| Fsp4HI | GCNGC | 3 cut(s) | 293, 367, 1212 |
| FspBI | CTAG | 3 cut(s) | 446, 876, 1260 |
| GluI | GCNGC | 3 cut(s) | 293, 367, 1212 |
| GsaI | CCCAGC | 1 cut(s) | 924 |
| GsuI | CTGGAG | 1 cut(s) | 76 |
| HaeIII | GGCC | 3 cut(s) | 224, 459, 1140 |
| HapII | CCGG | 3 cut(s) | 450, 845, 1215 |
| Hin1II | CATG | 6 cut(s) | 652, 705, 953, 1022, 1185, 1223 |
| HinfI | GANTC | 8 cut(s) | 100, 142, 287, 494, 583, 607, 631, 1151 |
| HpaII | CCGG | 3 cut(s) | 450, 845, 1215 |
| HphI | GGTGA | 3 cut(s) | 489, 819, 1093 |
| Hpy166II | GTNNAC | 2 cut(s) | 515, 884 |
| Hpy188I | TCNGA | 6 cut(s) | 191, 348, 588, 754, 801, 1015 |
| Hpy188III | TCNNGA | 6 cut(s) | 272, 393, 683, 876, 1055, 1220 |
| Hpy8I | GTNNAC | 2 cut(s) | 515, 884 |
| HpyAV | CCTTC | 6 cut(s) | 467, 665, 796, 800, 1078, 1275 |
| HpyCH4III | ACNGT | 5 cut(s) | 431, 901, 971, 977, 1114 |
| HpyCH4IV | ACGT | 1 cut(s) | 90 |
| HpyCH4V | TGCA | 9 cut(s) | 123, 170, 229, 369, 884, 1124, 1185, 1226, 1286 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 209 |
| HpyF3I | CTNAG | 6 cut(s) | 117, 644, 723, 864, 1001, 1089 |
| HpySE526I | ACGT | 1 cut(s) | 90 |
| Hsp92II | CATG | 6 cut(s) | 652, 705, 953, 1022, 1185, 1223 |
| Kzo9I | GATC | 3 cut(s) | 532, 679, 786 |
| LmnI | GCTCC | 2 cut(s) | 57, 1132 |
| Lsp1109I | GCAGC | 3 cut(s) | 304, 353, 1198 |
| LweI | GCATC | 2 cut(s) | 1213, 1273 |
| MabI | ACCWGGT | 1 cut(s) | 554 |
| MaeI | CTAG | 3 cut(s) | 446, 876, 1260 |
| MaeII | ACGT | 1 cut(s) | 90 |
| MaeIII | GTNAC | 5 cut(s) | 478, 691, 825, 965, 971 |
| MalI | GATC | 3 cut(s) | 534, 681, 788 |
| MboI | GATC | 3 cut(s) | 532, 679, 786 |
| MboII | GAAGA | 2 cut(s) | 560, 664 |
| MflI | RGATCY | 1 cut(s) | 786 |
| MhlI | GDGCHC | 3 cut(s) | 219, 886, 1142 |
| MluCI | AATT | 9 cut(s) | 184, 575, 708, 757, 772, 857, 986, 1093, 1195 |
| MlyI | GAGTC | 2 cut(s) | 109, 281 |
| MseI | TTAA | 6 cut(s) | 360, 380, 627, 906, 1097, 1175 |
| MslI | CAYNNNNRTG | 2 cut(s) | 700, 1023 |
| MspA1I | CMGCKG | 1 cut(s) | 295 |
| MspI | CCGG | 3 cut(s) | 450, 845, 1215 |
| MspR9I | CCNGG | 4 cut(s) | 55, 71, 556, 845 |
| Mva1269I | GAATGC | 3 cut(s) | 595, 661, 814 |
| MvaI | CCWGG | 3 cut(s) | 55, 71, 556 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 209 |
| NciI | CCSGG | 1 cut(s) | 845 |
| NcoI | CCATGG | 1 cut(s) | 1018 |
| NdeII | GATC | 3 cut(s) | 532, 679, 786 |
| NlaIII | CATG | 6 cut(s) | 652, 705, 953, 1022, 1185, 1223 |
| NlaIV | GGNNCC | 3 cut(s) | 788, 1140, 1164 |
| NmuCI | GTSAC | 4 cut(s) | 691, 825, 965, 971 |
| NspI | RCATGY | 2 cut(s) | 953, 1185 |
| NspV | TTCGAA | 1 cut(s) | 145 |
| PaeR7I | CTCGAG | 1 cut(s) | 391 |
| PagI | TCATGA | 1 cut(s) | 1219 |
| PasI | CCCWGGG | 1 cut(s) | 70 |
| PciI | ACATGT | 1 cut(s) | 949 |
| PctI | GAATGC | 3 cut(s) | 595, 661, 814 |
| PfeI | GAWTC | 6 cut(s) | 142, 494, 583, 607, 631, 1151 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 466 |
| PfoI | TCCNGGA | 2 cut(s) | 53, 843 |
| PkrI | GCNGC | 3 cut(s) | 294, 368, 1213 |
| PleI | GAGTC | 2 cut(s) | 108, 281 |
| PpsI | GAGTC | 2 cut(s) | 108, 281 |
| PpuMI | RGGWCCY | 1 cut(s) | 1162 |
| PscI | ACATGT | 1 cut(s) | 949 |
| Psp124BI | GAGCTC | 1 cut(s) | 219 |
| Psp5II | RGGWCCY | 1 cut(s) | 1162 |
| Psp6I | CCWGG | 3 cut(s) | 53, 69, 554 |
| PspFI | CCCAGC | 1 cut(s) | 920 |
| PspGI | CCWGG | 3 cut(s) | 53, 69, 554 |
| PspN4I | GGNNCC | 3 cut(s) | 788, 1140, 1164 |
| PspOMI | GGGCCC | 1 cut(s) | 1138 |
| PspPI | GGNCC | 5 cut(s) | 223, 457, 1138, 1139, 1162 |
| PspPPI | RGGWCCY | 1 cut(s) | 1162 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 507, 539 |
| PstNI | CAGNNNCTG | 1 cut(s) | 975 |
| PsuI | RGATCY | 1 cut(s) | 786 |
| PvuII | CAGCTG | 1 cut(s) | 295 |
| RsaI | GTAC | 3 cut(s) | 80, 332, 948 |
| RsaNI | GTAC | 3 cut(s) | 79, 331, 947 |
| RseI | CAYNNNNRTG | 2 cut(s) | 700, 1023 |
| SacI | GAGCTC | 1 cut(s) | 219 |
| SaqAI | TTAA | 6 cut(s) | 360, 380, 627, 906, 1097, 1175 |
| SatI | GCNGC | 3 cut(s) | 293, 367, 1212 |
| Sau3AI | GATC | 3 cut(s) | 532, 679, 786 |
| Sau96I | GGNCC | 5 cut(s) | 223, 457, 1138, 1139, 1162 |
| SchI | GAGTC | 2 cut(s) | 109, 281 |
| ScrFI | CCNGG | 4 cut(s) | 55, 71, 556, 845 |
| SduI | GDGCHC | 3 cut(s) | 219, 886, 1142 |
| SexAI | ACCWGGT | 1 cut(s) | 554 |
| SfaNI | GCATC | 2 cut(s) | 1213, 1273 |
| Sfr274I | CTCGAG | 1 cut(s) | 391 |
| SfuI | TTCGAA | 1 cut(s) | 145 |
| SinI | GGWCC | 1 cut(s) | 1162 |
| SlaI | CTCGAG | 1 cut(s) | 391 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 700, 1023 |
| SmlI | CTYRAG | 4 cut(s) | 218, 272, 391, 527 |
| SmoI | CTYRAG | 4 cut(s) | 218, 272, 391, 527 |
| Sse9I | AATT | 9 cut(s) | 184, 575, 708, 757, 772, 857, 986, 1093, 1195 |
| SsiI | CCGC | 2 cut(s) | 735, 784 |
| SspMI | CTAG | 3 cut(s) | 446, 876, 1260 |
| SstI | GAGCTC | 1 cut(s) | 219 |
| StyD4I | CCNGG | 4 cut(s) | 53, 69, 554, 843 |
| StyI | CCWWGG | 3 cut(s) | 611, 1018, 1062 |
| TaaI | ACNGT | 5 cut(s) | 431, 901, 971, 977, 1114 |
| TaiI | ACGT | 1 cut(s) | 93 |
| TaqI | TCGA | 6 cut(s) | 145, 314, 392, 550, 1068, 1149 |
| TasI | AATT | 9 cut(s) | 184, 575, 708, 757, 772, 857, 986, 1093, 1195 |
| TatI | WGTACW | 1 cut(s) | 946 |
| TfiI | GAWTC | 6 cut(s) | 142, 494, 583, 607, 631, 1151 |
| Tru1I | TTAA | 6 cut(s) | 360, 380, 627, 906, 1097, 1175 |
| Tru9I | TTAA | 6 cut(s) | 360, 380, 627, 906, 1097, 1175 |
| TscAI | CASTG | 4 cut(s) | 125, 906, 964, 976 |
| TseFI | GTSAC | 4 cut(s) | 691, 825, 965, 971 |
| TseI | GCWGC | 3 cut(s) | 292, 366, 1211 |
| Tsp45I | GTSAC | 4 cut(s) | 691, 825, 965, 971 |
| TspDTI | ATGAA | 4 cut(s) | 596, 665, 1064, 1257 |
| TspGWI | ACGGA | 1 cut(s) | 171 |
| TspRI | CASTG | 4 cut(s) | 125, 906, 964, 976 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 466 |
| VneI | GTGCAC | 1 cut(s) | 882 |
| VpaK11BI | GGWCC | 1 cut(s) | 1162 |
| XapI | RAATTY | 2 cut(s) | 986, 1093 |
| XbaI | TCTAGA | 1 cut(s) | 875 |
| XceI | RCATGY | 2 cut(s) | 953, 1185 |
| XhoI | CTCGAG | 1 cut(s) | 391 |
| XspI | CTAG | 3 cut(s) | 446, 876, 1260 |