ubiquitinyl hydrolase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1113 |
| AccB1I | GGYRCC | 3 cut(s) | 182, 861, 1171 |
| AccI | GTMKAC | 2 cut(s) | 63, 321 |
| AccIII | TCCGGA | 1 cut(s) | 357 |
| AciI | CCGC | 7 cut(s) | 11, 100, 437, 610, 727, 865, 1140 |
| AclWI | GGATC | 3 cut(s) | 633, 724, 737 |
| AcoI | YGGCCR | 1 cut(s) | 1739 |
| AcsI | RAATTY | 5 cut(s) | 830, 1053, 1523, 1752, 1759 |
| AcuI | CTGAAG | 3 cut(s) | 205, 578, 1562 |
| AcyI | GRCGYC | 1 cut(s) | 862 |
| AfaI | GTAC | 3 cut(s) | 776, 974, 1596 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 17, 179, 337, 769, 1748 |
| AflIII | ACRYGT | 1 cut(s) | 1257 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1261 |
| AjiI | CACGTC | 1 cut(s) | 1258 |
| AjnI | CCWGG | 1 cut(s) | 1512 |
| Alw21I | GWGCWC | 2 cut(s) | 1195, 1512 |
| Alw26I | GTCTC | 1 cut(s) | 1145 |
| Alw44I | GTGCAC | 1 cut(s) | 1191 |
| AlwI | GGATC | 3 cut(s) | 633, 724, 737 |
| Aor13HI | TCCGGA | 1 cut(s) | 357 |
| AoxI | GGCC | 4 cut(s) | 429, 1423, 1515, 1739 |
| ApaLI | GTGCAC | 1 cut(s) | 1191 |
| ApeKI | GCWGC | 4 cut(s) | 373, 443, 500, 1114 |
| ApoI | RAATTY | 5 cut(s) | 830, 1053, 1523, 1752, 1759 |
| AspLEI | GCGC | 3 cut(s) | 443, 864, 1114 |
| AspS9I | GGNCC | 2 cut(s) | 760, 1100 |
| AsuHPI | GGTGA | 4 cut(s) | 136, 311, 347, 725 |
| AvaII | GGWCC | 2 cut(s) | 760, 1100 |
| AxyI | CCTNAGG | 1 cut(s) | 1420 |
| BaeGI | GKGCMC | 1 cut(s) | 1195 |
| BamHI | GGATCC | 1 cut(s) | 729 |
| BanI | GGYRCC | 3 cut(s) | 182, 861, 1171 |
| BanII | GRGCYC | 1 cut(s) | 1512 |
| BbsI | GAAGAC | 2 cut(s) | 269, 399 |
| Bbv12I | GWGCWC | 2 cut(s) | 1195, 1512 |
| BbvI | GCAGC | 4 cut(s) | 360, 430, 487, 1126 |
| BccI | CCATC | 6 cut(s) | 87, 155, 224, 251, 904, 1699 |
| BceAI | ACGGC | 4 cut(s) | 416, 440, 813, 1726 |
| BciT130I | CCWGG | 1 cut(s) | 1514 |
| BciVI | GTATCC | 1 cut(s) | 750 |
| BcoDI | GTCTC | 1 cut(s) | 1145 |
| BfaI | CTAG | 5 cut(s) | 389, 504, 552, 1490, 1724 |
| BfoI | RGCGCY | 2 cut(s) | 444, 865 |
| BfuI | GTATCC | 1 cut(s) | 750 |
| BisI | GCNGC | 5 cut(s) | 374, 444, 501, 865, 1115 |
| BlpI | GCTNAGC | 1 cut(s) | 1653 |
| BlsI | GCNGC | 5 cut(s) | 375, 445, 502, 866, 1116 |
| Bme1390I | CCNGG | 1 cut(s) | 1514 |
| Bme18I | GGWCC | 2 cut(s) | 760, 1100 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1261 |
| BmgBI | CACGTC | 1 cut(s) | 1258 |
| BmgT120I | GGNCC | 2 cut(s) | 760, 1100 |
| BmiI | GGNNCC | 8 cut(s) | 22, 98, 184, 731, 863, 1071, 1101, 1173 |
| BmrFI | CCNGG | 1 cut(s) | 1514 |
| BmsI | GCATC | 3 cut(s) | 198, 717, 1018 |
| BpiI | GAAGAC | 2 cut(s) | 269, 399 |
| BpmI | CTGGAG | 1 cut(s) | 1496 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1653 |
| BpuEI | CTTGAG | 2 cut(s) | 1624, 1679 |
| BsaHI | GRCGYC | 1 cut(s) | 862 |
| BsaJI | CCNNGG | 6 cut(s) | 331, 432, 843, 1005, 1048, 1242 |
| BsaWI | WCCGGW | 1 cut(s) | 357 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1142, 1172 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 17, 179, 337, 769, 1748 |
| Bse1I | ACTGG | 2 cut(s) | 161, 245 |
| Bse21I | CCTNAGG | 1 cut(s) | 1420 |
| BseAI | TCCGGA | 1 cut(s) | 357 |
| BseBI | CCWGG | 1 cut(s) | 1514 |
| BseDI | CCNNGG | 6 cut(s) | 331, 432, 843, 1005, 1048, 1242 |
| BseGI | GGATG | 4 cut(s) | 64, 147, 472, 1624 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 17, 179, 337, 769, 1748 |
| BseNI | ACTGG | 2 cut(s) | 161, 245 |
| BseRI | GAGGAG | 1 cut(s) | 635 |
| BseSI | GKGCMC | 1 cut(s) | 1195 |
| BseXI | GCAGC | 4 cut(s) | 360, 430, 487, 1126 |
| BsgI | GTGCAG | 2 cut(s) | 493, 1503 |
| BshFI | GGCC | 4 cut(s) | 431, 1425, 1517, 1741 |
| BshNI | GGYRCC | 3 cut(s) | 182, 861, 1171 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1195, 1512 |
| BsiSI | CCGG | 1 cut(s) | 358 |
| BslFI | GGGAC | 1 cut(s) | 1113 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 17, 179, 337, 769, 1748 |
| BsmAI | GTCTC | 1 cut(s) | 1145 |
| BsmFI | GGGAC | 1 cut(s) | 1113 |
| BsmI | GAATGC | 2 cut(s) | 800, 1409 |
| BsnI | GGCC | 4 cut(s) | 431, 1425, 1517, 1741 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1195, 1512 |
| Bsp13I | TCCGGA | 1 cut(s) | 357 |
| Bsp143I | GATC | 2 cut(s) | 625, 729 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1653 |
| Bsp19I | CCATGG | 2 cut(s) | 331, 1048 |
| BspACI | CCGC | 7 cut(s) | 11, 100, 437, 610, 727, 865, 1140 |
| BspANI | GGCC | 4 cut(s) | 431, 1425, 1517, 1741 |
| BspEI | TCCGGA | 1 cut(s) | 357 |
| BspHI | TCATGA | 2 cut(s) | 280, 628 |
| BspLI | GGNNCC | 8 cut(s) | 22, 98, 184, 731, 863, 1071, 1101, 1173 |
| BspPI | GGATC | 3 cut(s) | 633, 724, 737 |
| BspQI | GCTCTTC | 2 cut(s) | 453, 1500 |
| BspT107I | GGYRCC | 3 cut(s) | 182, 861, 1171 |
| BsrI | ACTGG | 2 cut(s) | 161, 245 |
| BssECI | CCNNGG | 6 cut(s) | 331, 432, 843, 1005, 1048, 1242 |
| BssMI | GATC | 2 cut(s) | 625, 729 |
| BssNAI | GTATAC | 1 cut(s) | 322 |
| BssNI | GRCGYC | 1 cut(s) | 862 |
| BssT1I | CCWWGG | 5 cut(s) | 331, 843, 1005, 1048, 1242 |
| Bst1107I | GTATAC | 1 cut(s) | 322 |
| Bst2UI | CCWGG | 1 cut(s) | 1514 |
| Bst4CI | ACNGT | 9 cut(s) | 203, 268, 416, 637, 662, 1104, 1158, 1190, 1332 |
| Bst6I | CTCTTC | 5 cut(s) | 453, 1363, 1500, 1634, 1666 |
| BstACI | GRCGYC | 1 cut(s) | 862 |
| BstC8I | GCNNGC | 3 cut(s) | 439, 1176, 1180 |
| BstDEI | CTNAG | 6 cut(s) | 212, 566, 944, 1075, 1420, 1653 |
| BstDSI | CCRYGG | 3 cut(s) | 331, 432, 1048 |
| BstF5I | GGATG | 4 cut(s) | 64, 147, 472, 1624 |
| BstH2I | RGCGCY | 2 cut(s) | 444, 865 |
| BstHHI | GCGC | 3 cut(s) | 443, 864, 1114 |
| BstKTI | GATC | 2 cut(s) | 628, 732 |
| BstMAI | GTCTC | 1 cut(s) | 1145 |
| BstMBI | GATC | 2 cut(s) | 625, 729 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 443, 452, 1010, 1415 |
| BstNI | CCWGG | 1 cut(s) | 1514 |
| BstNSI | RCATGY | 1 cut(s) | 1065 |
| BstSCI | CCNGG | 1 cut(s) | 1512 |
| BstSLI | GKGCMC | 1 cut(s) | 1195 |
| BstV1I | GCAGC | 4 cut(s) | 360, 430, 487, 1126 |
| BstV2I | GAAGAC | 2 cut(s) | 269, 399 |
| BstX2I | RGATCY | 1 cut(s) | 729 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 813 |
| BstYI | RGATCY | 1 cut(s) | 729 |
| BstZ17I | GTATAC | 1 cut(s) | 322 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1420 |
| BsuI | GTATCC | 1 cut(s) | 750 |
| BsuRI | GGCC | 4 cut(s) | 431, 1425, 1517, 1741 |
| BtgI | CCRYGG | 3 cut(s) | 331, 432, 1048 |
| BtrI | CACGTC | 1 cut(s) | 1258 |
| BtsCI | GGATG | 4 cut(s) | 64, 147, 472, 1624 |
| BtsI | GCAGTG | 2 cut(s) | 481, 1020 |
| BtsIMutI | CAGTG | 6 cut(s) | 168, 481, 1020, 1195, 1215, 1328 |
| Cac8I | GCNNGC | 3 cut(s) | 439, 1176, 1180 |
| CciI | TCATGA | 2 cut(s) | 280, 628 |
| CfoI | GCGC | 3 cut(s) | 443, 864, 1114 |
| Cfr13I | GGNCC | 2 cut(s) | 760, 1100 |
| Csp6I | GTAC | 3 cut(s) | 775, 973, 1595 |
| CviQI | GTAC | 3 cut(s) | 775, 973, 1595 |
| DdeI | CTNAG | 6 cut(s) | 212, 566, 944, 1075, 1420, 1653 |
| DinI | GGCGCC | 1 cut(s) | 863 |
| DpnI | GATC | 2 cut(s) | 627, 731 |
| DpnII | GATC | 2 cut(s) | 625, 729 |
| DraI | TTTAAA | 1 cut(s) | 1399 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1261 |
| EaeI | YGGCCR | 1 cut(s) | 1739 |
| Eam1104I | CTCTTC | 5 cut(s) | 453, 1363, 1500, 1634, 1666 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1261 |
| EarI | CTCTTC | 5 cut(s) | 453, 1363, 1500, 1634, 1666 |
| EciI | GGCGGA | 2 cut(s) | 89, 742 |
| Ecl136II | GAGCTC | 1 cut(s) | 1510 |
| Eco130I | CCWWGG | 5 cut(s) | 331, 843, 1005, 1048, 1242 |
| Eco147I | AGGCCT | 1 cut(s) | 1517 |
| Eco24I | GRGCYC | 1 cut(s) | 1512 |
| Eco47I | GGWCC | 2 cut(s) | 760, 1100 |
| Eco53kI | GAGCTC | 1 cut(s) | 1510 |
| Eco57I | CTGAAG | 3 cut(s) | 205, 578, 1562 |
| Eco81I | CCTNAGG | 1 cut(s) | 1420 |
| EcoICRI | GAGCTC | 1 cut(s) | 1510 |
| EcoRI | GAATTC | 1 cut(s) | 1752 |
| EcoRII | CCWGG | 1 cut(s) | 1512 |
| EcoT14I | CCWWGG | 5 cut(s) | 331, 843, 1005, 1048, 1242 |
| EcoT22I | ATGCAT | 2 cut(s) | 121, 995 |
| EcoT38I | GRGCYC | 1 cut(s) | 1512 |
| EgeI | GGCGCC | 1 cut(s) | 863 |
| EheI | GGCGCC | 1 cut(s) | 863 |
| ErhI | CCWWGG | 5 cut(s) | 331, 843, 1005, 1048, 1242 |
| FaqI | GGGAC | 1 cut(s) | 1113 |
| FauI | CCCGC | 1 cut(s) | 430 |
| FblI | GTMKAC | 2 cut(s) | 63, 321 |
| Fnu4HI | GCNGC | 5 cut(s) | 374, 444, 501, 865, 1115 |
| FokI | GGATG | 4 cut(s) | 71, 134, 479, 1631 |
| FriOI | GRGCYC | 1 cut(s) | 1512 |
| Fsp4HI | GCNGC | 5 cut(s) | 374, 444, 501, 865, 1115 |
| FspBI | CTAG | 5 cut(s) | 389, 504, 552, 1490, 1724 |
| FspI | TGCGCA | 1 cut(s) | 1113 |
| GlaI | GCGC | 3 cut(s) | 442, 863, 1113 |
| GluI | GCNGC | 5 cut(s) | 374, 444, 501, 865, 1115 |
| GsuI | CTGGAG | 1 cut(s) | 1496 |
| HaeII | RGCGCY | 2 cut(s) | 444, 865 |
| HaeIII | GGCC | 4 cut(s) | 431, 1425, 1517, 1741 |
| HapII | CCGG | 1 cut(s) | 358 |
| HhaI | GCGC | 3 cut(s) | 443, 864, 1114 |
| Hin1I | GRCGYC | 1 cut(s) | 862 |
| Hin6I | GCGC | 3 cut(s) | 441, 862, 1112 |
| HinP1I | GCGC | 3 cut(s) | 441, 862, 1112 |
| HincII | GTYRAC | 1 cut(s) | 64 |
| HindII | GTYRAC | 1 cut(s) | 64 |
| HindIII | AAGCTT | 1 cut(s) | 1655 |
| HinfI | GANTC | 6 cut(s) | 28, 275, 385, 538, 562, 886 |
| HpaII | CCGG | 1 cut(s) | 358 |
| HphI | GGTGA | 4 cut(s) | 136, 311, 347, 725 |
| Hpy166II | GTNNAC | 8 cut(s) | 64, 166, 322, 578, 775, 1166, 1193, 1266 |
| Hpy188I | TCNGA | 5 cut(s) | 25, 588, 697, 1204, 1758 |
| Hpy188III | TCNNGA | 6 cut(s) | 223, 281, 358, 389, 629, 1130 |
| Hpy8I | GTNNAC | 8 cut(s) | 64, 166, 322, 578, 775, 1166, 1193, 1266 |
| HpyAV | CCTTC | 9 cut(s) | 457, 517, 583, 691, 1075, 1085, 1232, 1528, 1685 |
| HpyCH4III | ACNGT | 9 cut(s) | 203, 268, 416, 637, 662, 1104, 1158, 1190, 1332 |
| HpyCH4IV | ACGT | 1 cut(s) | 1257 |
| HpyCH4V | TGCA | 9 cut(s) | 49, 119, 474, 993, 1013, 1031, 1061, 1193, 1484 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 443, 452, 1010, 1415 |
| HpyF3I | CTNAG | 6 cut(s) | 212, 566, 944, 1075, 1420, 1653 |
| HpySE526I | ACGT | 1 cut(s) | 1257 |
| Hsp92I | GRCGYC | 1 cut(s) | 862 |
| HspAI | GCGC | 3 cut(s) | 441, 862, 1112 |
| KasI | GGCGCC | 1 cut(s) | 861 |
| Kpn2I | TCCGGA | 1 cut(s) | 357 |
| Kzo9I | GATC | 2 cut(s) | 625, 729 |
| LguI | GCTCTTC | 2 cut(s) | 453, 1500 |
| LmnI | GCTCC | 3 cut(s) | 102, 487, 1515 |
| Lsp1109I | GCAGC | 4 cut(s) | 360, 430, 487, 1126 |
| LweI | GCATC | 3 cut(s) | 198, 717, 1018 |
| MaeI | CTAG | 5 cut(s) | 389, 504, 552, 1490, 1724 |
| MaeII | ACGT | 1 cut(s) | 1257 |
| MaeIII | GTNAC | 2 cut(s) | 637, 1352 |
| MalI | GATC | 2 cut(s) | 627, 731 |
| MboI | GATC | 2 cut(s) | 625, 729 |
| MfeI | CAATTG | 1 cut(s) | 1145 |
| MflI | RGATCY | 1 cut(s) | 729 |
| MhlI | GDGCHC | 2 cut(s) | 1195, 1512 |
| Mly113I | GGCGCC | 1 cut(s) | 862 |
| MlyI | GAGTC | 1 cut(s) | 379 |
| Mph1103I | ATGCAT | 2 cut(s) | 121, 995 |
| MroI | TCCGGA | 1 cut(s) | 357 |
| MseI | TTAA | 7 cut(s) | 657, 839, 849, 1251, 1368, 1398, 1458 |
| MslI | CAYNNNNRTG | 3 cut(s) | 71, 414, 646 |
| MspA1I | CMGCKG | 1 cut(s) | 1274 |
| MspI | CCGG | 1 cut(s) | 358 |
| MspR9I | CCNGG | 1 cut(s) | 1514 |
| MunI | CAATTG | 1 cut(s) | 1145 |
| Mva1269I | GAATGC | 2 cut(s) | 800, 1409 |
| MvaI | CCWGG | 1 cut(s) | 1514 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 443, 452, 1010, 1415 |
| NarI | GGCGCC | 1 cut(s) | 862 |
| NcoI | CCATGG | 2 cut(s) | 331, 1048 |
| NdeII | GATC | 2 cut(s) | 625, 729 |
| NlaIV | GGNNCC | 8 cut(s) | 22, 98, 184, 731, 863, 1071, 1101, 1173 |
| NmeAIII | GCCGAG | 1 cut(s) | 1451 |
| NmuCI | GTSAC | 2 cut(s) | 637, 1352 |
| NsbI | TGCGCA | 1 cut(s) | 1113 |
| NsiI | ATGCAT | 2 cut(s) | 121, 995 |
| NspI | RCATGY | 1 cut(s) | 1065 |
| PagI | TCATGA | 2 cut(s) | 280, 628 |
| PceI | AGGCCT | 1 cut(s) | 1517 |
| PciSI | GCTCTTC | 2 cut(s) | 453, 1500 |
| PctI | GAATGC | 2 cut(s) | 800, 1409 |
| PfeI | GAWTC | 5 cut(s) | 28, 275, 538, 562, 886 |
| PkrI | GCNGC | 5 cut(s) | 375, 445, 502, 866, 1116 |
| PleI | GAGTC | 1 cut(s) | 379 |
| PluTI | GGCGCC | 1 cut(s) | 865 |
| PpsI | GAGTC | 1 cut(s) | 379 |
| Psp124BI | GAGCTC | 1 cut(s) | 1512 |
| Psp6I | CCWGG | 1 cut(s) | 1512 |
| PspGI | CCWGG | 1 cut(s) | 1512 |
| PspN4I | GGNNCC | 8 cut(s) | 22, 98, 184, 731, 863, 1071, 1101, 1173 |
| PspPI | GGNCC | 2 cut(s) | 760, 1100 |
| PsuI | RGATCY | 1 cut(s) | 729 |
| PvuII | CAGCTG | 1 cut(s) | 1274 |
| RsaI | GTAC | 3 cut(s) | 776, 974, 1596 |
| RsaNI | GTAC | 3 cut(s) | 775, 973, 1595 |
| RseI | CAYNNNNRTG | 3 cut(s) | 71, 414, 646 |
| SacI | GAGCTC | 1 cut(s) | 1512 |
| SalI | GTCGAC | 1 cut(s) | 62 |
| SapI | GCTCTTC | 2 cut(s) | 453, 1500 |
| SaqAI | TTAA | 7 cut(s) | 657, 839, 849, 1251, 1368, 1398, 1458 |
| SatI | GCNGC | 5 cut(s) | 374, 444, 501, 865, 1115 |
| Sau3AI | GATC | 2 cut(s) | 625, 729 |
| Sau96I | GGNCC | 2 cut(s) | 760, 1100 |
| SchI | GAGTC | 1 cut(s) | 379 |
| ScrFI | CCNGG | 1 cut(s) | 1514 |
| SduI | GDGCHC | 2 cut(s) | 1195, 1512 |
| SfaNI | GCATC | 3 cut(s) | 198, 717, 1018 |
| SfoI | GGCGCC | 1 cut(s) | 863 |
| SinI | GGWCC | 2 cut(s) | 760, 1100 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 71, 414, 646 |
| SmlI | CTYRAG | 2 cut(s) | 1603, 1658 |
| SmoI | CTYRAG | 2 cut(s) | 1603, 1658 |
| SseBI | AGGCCT | 1 cut(s) | 1517 |
| SsiI | CCGC | 7 cut(s) | 11, 100, 437, 610, 727, 865, 1140 |
| SspDI | GGCGCC | 1 cut(s) | 861 |
| SspMI | CTAG | 5 cut(s) | 389, 504, 552, 1490, 1724 |
| SstI | GAGCTC | 1 cut(s) | 1512 |
| StuI | AGGCCT | 1 cut(s) | 1517 |
| StyD4I | CCNGG | 1 cut(s) | 1512 |
| StyI | CCWWGG | 5 cut(s) | 331, 843, 1005, 1048, 1242 |
| TaaI | ACNGT | 9 cut(s) | 203, 268, 416, 637, 662, 1104, 1158, 1190, 1332 |
| TaiI | ACGT | 1 cut(s) | 1260 |
| TaqI | TCGA | 2 cut(s) | 63, 1689 |
| TatI | WGTACW | 2 cut(s) | 774, 1594 |
| TauI | GCSGC | 1 cut(s) | 867 |
| TfiI | GAWTC | 5 cut(s) | 28, 275, 538, 562, 886 |
| Tru1I | TTAA | 7 cut(s) | 657, 839, 849, 1251, 1368, 1398, 1458 |
| Tru9I | TTAA | 7 cut(s) | 657, 839, 849, 1251, 1368, 1398, 1458 |
| TscAI | CASTG | 6 cut(s) | 168, 481, 1020, 1195, 1222, 1335 |
| TseFI | GTSAC | 2 cut(s) | 637, 1352 |
| TseI | GCWGC | 4 cut(s) | 373, 443, 500, 1114 |
| Tsp45I | GTSAC | 2 cut(s) | 637, 1352 |
| TspRI | CASTG | 6 cut(s) | 168, 481, 1020, 1195, 1222, 1335 |
| VneI | GTGCAC | 1 cut(s) | 1191 |
| VpaK11BI | GGWCC | 2 cut(s) | 760, 1100 |
| XapI | RAATTY | 5 cut(s) | 830, 1053, 1523, 1752, 1759 |
| XbaI | TCTAGA | 1 cut(s) | 388 |
| XceI | RCATGY | 1 cut(s) | 1065 |
| XmiI | GTMKAC | 2 cut(s) | 63, 321 |
| XspI | CTAG | 5 cut(s) | 389, 504, 552, 1490, 1724 |
| Zsp2I | ATGCAT | 2 cut(s) | 121, 995 |