No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 866 |
| Acc16I | TGCGCA | 1 cut(s) | 962 |
| Acc36I | ACCTGC | 1 cut(s) | 866 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 116 |
| AciI | CCGC | 2 cut(s) | 1022, 1207 |
| AclWI | GGATC | 1 cut(s) | 311 |
| AcsI | RAATTY | 2 cut(s) | 347, 1086 |
| AfaI | GTAC | 1 cut(s) | 575 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 116, 762 |
| AflII | CTTAAG | 1 cut(s) | 611 |
| AflIII | ACRYGT | 1 cut(s) | 570 |
| AgsI | TTSAA | 7 cut(s) | 284, 295, 353, 461, 470, 478, 922 |
| AjnI | CCWGG | 1 cut(s) | 141 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 176, 208 |
| AluBI | AGCT | 4 cut(s) | 74, 212, 632, 1195 |
| AluI | AGCT | 4 cut(s) | 74, 212, 632, 1195 |
| Alw21I | GWGCWC | 2 cut(s) | 253, 662 |
| Alw26I | GTCTC | 1 cut(s) | 26 |
| Alw44I | GTGCAC | 1 cut(s) | 658 |
| AlwI | GGATC | 1 cut(s) | 311 |
| ApaLI | GTGCAC | 1 cut(s) | 658 |
| ApeKI | GCWGC | 6 cut(s) | 138, 390, 393, 632, 643, 1253 |
| ApoI | RAATTY | 2 cut(s) | 347, 1086 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 942 |
| AspLEI | GCGC | 1 cut(s) | 963 |
| AspS9I | GGNCC | 1 cut(s) | 1209 |
| AsuC2I | CCSGG | 1 cut(s) | 588 |
| AsuHPI | GGTGA | 4 cut(s) | 131, 463, 530, 1036 |
| AvaII | GGWCC | 1 cut(s) | 1209 |
| BaeGI | GKGCMC | 1 cut(s) | 662 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 698, 731 |
| BbsI | GAAGAC | 3 cut(s) | 477, 900, 1112 |
| Bbv12I | GWGCWC | 2 cut(s) | 253, 662 |
| BbvCI | CCTCAGC | 1 cut(s) | 272 |
| BbvI | GCAGC | 6 cut(s) | 150, 402, 405, 619, 630, 1240 |
| BccI | CCATC | 1 cut(s) | 631 |
| BciT130I | CCWGG | 1 cut(s) | 143 |
| BciVI | GTATCC | 1 cut(s) | 660 |
| BclI | TGATCA | 1 cut(s) | 882 |
| BcnI | CCSGG | 1 cut(s) | 588 |
| BcoDI | GTCTC | 1 cut(s) | 26 |
| BfaI | CTAG | 2 cut(s) | 135, 663 |
| BfmI | CTRYAG | 1 cut(s) | 1254 |
| BfrI | CTTAAG | 1 cut(s) | 611 |
| BfuAI | ACCTGC | 1 cut(s) | 866 |
| BfuI | GTATCC | 1 cut(s) | 660 |
| BisI | GCNGC | 6 cut(s) | 139, 391, 394, 633, 644, 1254 |
| BlsI | GCNGC | 6 cut(s) | 140, 392, 395, 634, 645, 1255 |
| Bme1390I | CCNGG | 2 cut(s) | 143, 588 |
| Bme18I | GGWCC | 1 cut(s) | 1209 |
| BmgT120I | GGNCC | 1 cut(s) | 1209 |
| BmrFI | CCNGG | 2 cut(s) | 143, 588 |
| BmsI | GCATC | 2 cut(s) | 117, 1026 |
| BpiI | GAAGAC | 3 cut(s) | 477, 900, 1112 |
| BpmI | CTGGAG | 2 cut(s) | 182, 544 |
| Bpu10I | CCTNAGC | 2 cut(s) | 272, 914 |
| BpuMI | CCSGG | 1 cut(s) | 588 |
| BsaBI | GATNNNNATC | 1 cut(s) | 622 |
| BsaJI | CCNNGG | 1 cut(s) | 826 |
| BsaWI | WCCGGW | 1 cut(s) | 515 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 116, 762 |
| Bse1I | ACTGG | 1 cut(s) | 165 |
| Bse3DI | GCAATG | 1 cut(s) | 178 |
| Bse8I | GATNNNNATC | 1 cut(s) | 622 |
| BseBI | CCWGG | 1 cut(s) | 143 |
| BseDI | CCNNGG | 1 cut(s) | 826 |
| BseGI | GGATG | 8 cut(s) | 43, 55, 97, 132, 346, 370, 873, 1041 |
| BseJI | GATNNNNATC | 1 cut(s) | 622 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 116, 762 |
| BseMI | GCAATG | 1 cut(s) | 178 |
| BseMII | CTCAG | 1 cut(s) | 263 |
| BseNI | ACTGG | 1 cut(s) | 165 |
| BseRI | GAGGAG | 6 cut(s) | 44, 80, 248, 289, 573, 1091 |
| BseSI | GKGCMC | 1 cut(s) | 662 |
| BseXI | GCAGC | 6 cut(s) | 150, 402, 405, 619, 630, 1240 |
| BsgI | GTGCAG | 1 cut(s) | 409 |
| Bsh1285I | CGRYCG | 1 cut(s) | 891 |
| BsiEI | CGRYCG | 1 cut(s) | 891 |
| BsiHKAI | GWGCWC | 2 cut(s) | 253, 662 |
| BsiSI | CCGG | 2 cut(s) | 516, 587 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 116, 762 |
| BsmAI | GTCTC | 1 cut(s) | 26 |
| BsmBI | CGTCTC | 1 cut(s) | 26 |
| BsmI | GAATGC | 2 cut(s) | 467, 1006 |
| Bsp1286I | GDGCHC | 2 cut(s) | 253, 662 |
| Bsp143I | GATC | 3 cut(s) | 303, 617, 882 |
| BspACI | CCGC | 2 cut(s) | 1022, 1207 |
| BspCNI | CTCAG | 1 cut(s) | 264 |
| BspHI | TCATGA | 1 cut(s) | 834 |
| BspMAI | CTGCAG | 1 cut(s) | 1258 |
| BspMI | ACCTGC | 1 cut(s) | 866 |
| BspPI | GGATC | 1 cut(s) | 311 |
| BspTI | CTTAAG | 1 cut(s) | 611 |
| BsrDI | GCAATG | 1 cut(s) | 178 |
| BsrI | ACTGG | 1 cut(s) | 165 |
| BssECI | CCNNGG | 1 cut(s) | 826 |
| BssMI | GATC | 3 cut(s) | 303, 617, 882 |
| BssT1I | CCWWGG | 1 cut(s) | 826 |
| Bst2UI | CCWGG | 1 cut(s) | 143 |
| Bst6I | CTCTTC | 4 cut(s) | 39, 293, 483, 728 |
| BstAFI | CTTAAG | 1 cut(s) | 611 |
| BstC8I | GCNNGC | 3 cut(s) | 103, 311, 398 |
| BstDEI | CTNAG | 3 cut(s) | 272, 761, 914 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 760 |
| BstF5I | GGATG | 8 cut(s) | 43, 55, 97, 132, 346, 370, 873, 1041 |
| BstHHI | GCGC | 1 cut(s) | 963 |
| BstKTI | GATC | 3 cut(s) | 306, 620, 885 |
| BstMAI | GTCTC | 1 cut(s) | 26 |
| BstMBI | GATC | 3 cut(s) | 303, 617, 882 |
| BstMCI | CGRYCG | 1 cut(s) | 891 |
| BstNI | CCWGG | 1 cut(s) | 143 |
| BstNSI | RCATGY | 2 cut(s) | 400, 574 |
| BstSCI | CCNGG | 2 cut(s) | 141, 586 |
| BstSFI | CTRYAG | 1 cut(s) | 1254 |
| BstSLI | GKGCMC | 1 cut(s) | 662 |
| BstV1I | GCAGC | 6 cut(s) | 150, 402, 405, 619, 630, 1240 |
| BstV2I | GAAGAC | 3 cut(s) | 477, 900, 1112 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1012 |
| BsuI | GTATCC | 1 cut(s) | 660 |
| BtgZI | GCGATG | 1 cut(s) | 27 |
| BtsCI | GGATG | 8 cut(s) | 43, 55, 97, 132, 346, 370, 873, 1041 |
| BtsI | GCAGTG | 1 cut(s) | 1017 |
| BtsIMutI | CAGTG | 1 cut(s) | 1017 |
| BveI | ACCTGC | 1 cut(s) | 866 |
| Cac8I | GCNNGC | 3 cut(s) | 103, 311, 398 |
| CciI | TCATGA | 1 cut(s) | 834 |
| CfoI | GCGC | 1 cut(s) | 963 |
| Cfr13I | GGNCC | 1 cut(s) | 1209 |
| Csp6I | GTAC | 1 cut(s) | 574 |
| CviAII | CATG | 5 cut(s) | 181, 397, 571, 647, 835 |
| CviQI | GTAC | 1 cut(s) | 574 |
| DdeI | CTNAG | 3 cut(s) | 272, 761, 914 |
| DpnI | GATC | 3 cut(s) | 305, 619, 884 |
| DpnII | GATC | 3 cut(s) | 303, 617, 882 |
| DraI | TTTAAA | 1 cut(s) | 216 |
| Eam1104I | CTCTTC | 4 cut(s) | 39, 293, 483, 728 |
| EarI | CTCTTC | 4 cut(s) | 39, 293, 483, 728 |
| Eco130I | CCWWGG | 1 cut(s) | 826 |
| Eco47I | GGWCC | 1 cut(s) | 1209 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 760 |
| EcoRII | CCWGG | 1 cut(s) | 141 |
| EcoT14I | CCWWGG | 1 cut(s) | 826 |
| ErhI | CCWWGG | 1 cut(s) | 826 |
| Esp3I | CGTCTC | 1 cut(s) | 26 |
| FaeI | CATG | 5 cut(s) | 184, 400, 574, 650, 838 |
| FatI | CATG | 5 cut(s) | 180, 396, 570, 646, 834 |
| FauNDI | CATATG | 1 cut(s) | 844 |
| FbaI | TGATCA | 1 cut(s) | 882 |
| Fnu4HI | GCNGC | 6 cut(s) | 139, 391, 394, 633, 644, 1254 |
| FokI | GGATG | 8 cut(s) | 50, 62, 104, 139, 353, 377, 860, 1048 |
| Fsp4HI | GCNGC | 6 cut(s) | 139, 391, 394, 633, 644, 1254 |
| FspBI | CTAG | 2 cut(s) | 135, 663 |
| FspI | TGCGCA | 1 cut(s) | 962 |
| GlaI | GCGC | 1 cut(s) | 962 |
| GluI | GCNGC | 6 cut(s) | 139, 391, 394, 633, 644, 1254 |
| GsuI | CTGGAG | 2 cut(s) | 182, 544 |
| HapII | CCGG | 2 cut(s) | 516, 587 |
| HhaI | GCGC | 1 cut(s) | 963 |
| Hin1II | CATG | 5 cut(s) | 184, 400, 574, 650, 838 |
| Hin6I | GCGC | 1 cut(s) | 961 |
| HinP1I | GCGC | 1 cut(s) | 961 |
| HincII | GTYRAC | 1 cut(s) | 1290 |
| HindII | GTYRAC | 1 cut(s) | 1290 |
| HindIII | AAGCTT | 1 cut(s) | 210 |
| HinfI | GANTC | 6 cut(s) | 280, 406, 557, 651, 831, 943 |
| HpaII | CCGG | 2 cut(s) | 516, 587 |
| HphI | GGTGA | 4 cut(s) | 131, 463, 530, 1036 |
| Hpy166II | GTNNAC | 2 cut(s) | 660, 1290 |
| Hpy188I | TCNGA | 1 cut(s) | 411 |
| Hpy188III | TCNNGA | 3 cut(s) | 835, 886, 977 |
| Hpy8I | GTNNAC | 2 cut(s) | 660, 1290 |
| HpyAV | CCTTC | 3 cut(s) | 77, 178, 922 |
| HpyCH4IV | ACGT | 1 cut(s) | 1031 |
| HpyCH4V | TGCA | 8 cut(s) | 390, 400, 635, 646, 660, 1057, 1145, 1256 |
| HpyF3I | CTNAG | 3 cut(s) | 272, 761, 914 |
| HpySE526I | ACGT | 1 cut(s) | 1031 |
| Hsp92II | CATG | 5 cut(s) | 184, 400, 574, 650, 838 |
| HspAI | GCGC | 1 cut(s) | 961 |
| Ksp22I | TGATCA | 1 cut(s) | 882 |
| Kzo9I | GATC | 3 cut(s) | 303, 617, 882 |
| Lsp1109I | GCAGC | 6 cut(s) | 150, 402, 405, 619, 630, 1240 |
| LweI | GCATC | 2 cut(s) | 117, 1026 |
| MaeI | CTAG | 2 cut(s) | 135, 663 |
| MaeII | ACGT | 1 cut(s) | 1031 |
| MaeIII | GTNAC | 2 cut(s) | 714, 1024 |
| MalI | GATC | 3 cut(s) | 305, 619, 884 |
| MboI | GATC | 3 cut(s) | 303, 617, 882 |
| MhlI | GDGCHC | 2 cut(s) | 253, 662 |
| MluCI | AATT | 3 cut(s) | 238, 347, 1086 |
| MlyI | GAGTC | 2 cut(s) | 551, 660 |
| MmeI | TCCRAC | 3 cut(s) | 174, 831, 1180 |
| MroXI | GAANNNNTTC | 1 cut(s) | 942 |
| MseI | TTAA | 3 cut(s) | 215, 612, 1190 |
| MslI | CAYNNNNRTG | 1 cut(s) | 59 |
| MspCI | CTTAAG | 1 cut(s) | 611 |
| MspI | CCGG | 2 cut(s) | 516, 587 |
| MspR9I | CCNGG | 2 cut(s) | 143, 588 |
| Mva1269I | GAATGC | 2 cut(s) | 467, 1006 |
| MvaI | CCWGG | 1 cut(s) | 143 |
| NciI | CCSGG | 1 cut(s) | 588 |
| NdeI | CATATG | 1 cut(s) | 844 |
| NdeII | GATC | 3 cut(s) | 303, 617, 882 |
| NlaIII | CATG | 5 cut(s) | 184, 400, 574, 650, 838 |
| NmuCI | GTSAC | 1 cut(s) | 1024 |
| NsbI | TGCGCA | 1 cut(s) | 962 |
| NspI | RCATGY | 2 cut(s) | 400, 574 |
| PaeI | GCATGC | 1 cut(s) | 400 |
| PagI | TCATGA | 1 cut(s) | 834 |
| PaqCI | CACCTGC | 1 cut(s) | 866 |
| PciI | ACATGT | 1 cut(s) | 570 |
| PctI | GAATGC | 2 cut(s) | 467, 1006 |
| PdmI | GAANNNNTTC | 1 cut(s) | 942 |
| PfeI | GAWTC | 4 cut(s) | 280, 406, 831, 943 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 116 |
| PfoI | TCCNGGA | 1 cut(s) | 586 |
| PkrI | GCNGC | 6 cut(s) | 140, 392, 395, 634, 645, 1255 |
| PleI | GAGTC | 2 cut(s) | 551, 659 |
| PpsI | GAGTC | 2 cut(s) | 551, 659 |
| PscI | ACATGT | 1 cut(s) | 570 |
| Psp6I | CCWGG | 1 cut(s) | 141 |
| PspGI | CCWGG | 1 cut(s) | 141 |
| PspPI | GGNCC | 1 cut(s) | 1209 |
| PstI | CTGCAG | 1 cut(s) | 1258 |
| RsaI | GTAC | 1 cut(s) | 575 |
| RsaNI | GTAC | 1 cut(s) | 574 |
| RseI | CAYNNNNRTG | 1 cut(s) | 59 |
| SaqAI | TTAA | 3 cut(s) | 215, 612, 1190 |
| SatI | GCNGC | 6 cut(s) | 139, 391, 394, 633, 644, 1254 |
| Sau3AI | GATC | 3 cut(s) | 303, 617, 882 |
| Sau96I | GGNCC | 1 cut(s) | 1209 |
| SchI | GAGTC | 2 cut(s) | 551, 660 |
| ScrFI | CCNGG | 2 cut(s) | 143, 588 |
| SduI | GDGCHC | 2 cut(s) | 253, 662 |
| SfaNI | GCATC | 2 cut(s) | 117, 1026 |
| SfcI | CTRYAG | 1 cut(s) | 1254 |
| SinI | GGWCC | 1 cut(s) | 1209 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 59 |
| SmlI | CTYRAG | 1 cut(s) | 611 |
| SmoI | CTYRAG | 1 cut(s) | 611 |
| SphI | GCATGC | 1 cut(s) | 400 |
| Sse9I | AATT | 3 cut(s) | 238, 347, 1086 |
| SsiI | CCGC | 2 cut(s) | 1022, 1207 |
| SspMI | CTAG | 2 cut(s) | 135, 663 |
| StyD4I | CCNGG | 2 cut(s) | 141, 586 |
| StyI | CCWWGG | 1 cut(s) | 826 |
| TaiI | ACGT | 1 cut(s) | 1034 |
| TaqI | TCGA | 2 cut(s) | 316, 1093 |
| TaqII | GACCGA | 1 cut(s) | 905 |
| TasI | AATT | 3 cut(s) | 238, 347, 1086 |
| TfiI | GAWTC | 4 cut(s) | 280, 406, 831, 943 |
| Tru1I | TTAA | 3 cut(s) | 215, 612, 1190 |
| Tru9I | TTAA | 3 cut(s) | 215, 612, 1190 |
| TscAI | CASTG | 1 cut(s) | 1017 |
| TseFI | GTSAC | 1 cut(s) | 1024 |
| TseI | GCWGC | 6 cut(s) | 138, 390, 393, 632, 643, 1253 |
| Tsp45I | GTSAC | 1 cut(s) | 1024 |
| TspGWI | ACGGA | 1 cut(s) | 50 |
| TspRI | CASTG | 1 cut(s) | 1017 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 116 |
| Vha464I | CTTAAG | 1 cut(s) | 611 |
| VneI | GTGCAC | 1 cut(s) | 658 |
| VpaK11BI | GGWCC | 1 cut(s) | 1209 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 760 |
| XapI | RAATTY | 2 cut(s) | 347, 1086 |
| XceI | RCATGY | 2 cut(s) | 400, 574 |
| XmnI | GAANNNNTTC | 1 cut(s) | 942 |
| XspI | CTAG | 2 cut(s) | 135, 663 |