FvH4_2g34510

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000732
Physical Location & Seq
Forward (+)
25624388 .. 25625056
669 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34510.t1

Sequence Viewer

Length: 303 bp
ATGAGGTTTGTCCAATATCCCTTCACACCGGTCCTCTGCTGGCCTCATGATAAGAAGGTCCCTGCAGCTCAGTGGAGCCCGGAGCTGGTCCGTGATCTTTTCAAAAGCGCTGACAAGAACGGTGACGGCAAACTCTCCAAGGAGGAGCTCAAGGCAGCCTTTCGGAGACTCGGATCGCGTTGGAGCTCTTACAGAGCTAGGAGAGCTTTACGCCATGTAGATGCCAACGGCGACGGCGTCATCTCTAATGAGGAGCTCAACGACCTTATCAACTATGCCCTACAATGTGGTTATAAGCTCTAA

Protein Analysis

101

Amino Acids

11.51

Weight (kDa)

9.43

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 30 - 57 7.9e-08 EF hand domain
EF-hand_6 PF13405 30 - 58 5.2e-08 EF-hand domain
EF-hand_5 PF13202 31 - 52 5.1e-08 EF hand
EF-hand_7 PF13499 31 - 87 5.4e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AccII CGCG 1 cut(s) 178
AclWI GGATC 1 cut(s) 181
AcyI GRCGYC 1 cut(s) 237
AfeI AGCGCT 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 85
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 1 cut(s) 103
AluBI AGCT 8 cut(s) 68, 85, 148, 186, 197, 206, 256, 298
AluI AGCT 8 cut(s) 68, 85, 148, 186, 197, 206, 256, 298
Alw21I GWGCWC 3 cut(s) 150, 188, 258
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 65, 155
AsiGI ACCGGT 1 cut(s) 28
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 3 cut(s) 31, 58, 88
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 3 cut(s) 31, 58, 88
BanII GRGCYC 4 cut(s) 80, 150, 188, 258
Bbv12I GWGCWC 3 cut(s) 150, 188, 258
BbvI GCAGC 2 cut(s) 77, 167
BceAI ACGGC 3 cut(s) 142, 244, 250
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 198
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 2 cut(s) 66, 156
BlsI GCNGC 2 cut(s) 67, 157
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 3 cut(s) 31, 58, 88
BmgT120I GGNCC 3 cut(s) 31, 58, 88
BmiI GGNNCC 2 cut(s) 60, 77
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 83
BseRI GAGGAG 2 cut(s) 158, 266
BseXI GCAGC 2 cut(s) 77, 167
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 1 cut(s) 43
BshTI ACCGGT 1 cut(s) 28
BsiHKAI GWGCWC 3 cut(s) 150, 188, 258
BsiSI CCGG 2 cut(s) 29, 80
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 4 cut(s) 80, 150, 188, 258
Bsp143I GATC 2 cut(s) 94, 173
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 60, 77
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 2 cut(s) 94, 173
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 2 cut(s) 97, 176
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 2 cut(s) 94, 173
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 77, 167
BsuRI GGCC 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 46
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 31, 58, 88
CseI GACGC 1 cut(s) 226
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 2 cut(s) 47, 215
DdeI CTNAG 1 cut(s) 69
DpnI GATC 2 cut(s) 96, 175
DpnII GATC 2 cut(s) 94, 173
Ecl136II GAGCTC 3 cut(s) 148, 186, 256
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 4 cut(s) 80, 150, 188, 258
Eco47I GGWCC 3 cut(s) 31, 58, 88
Eco47III AGCGCT 1 cut(s) 109
Eco53kI GAGCTC 3 cut(s) 148, 186, 256
EcoICRI GAGCTC 3 cut(s) 148, 186, 256
EcoO109I RGGNCCY 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 4 cut(s) 80, 150, 188, 258
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 2 cut(s) 50, 218
FaiI YATR 4 cut(s) 48, 216, 276, 294
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 1 cut(s) 44
FatI CATG 2 cut(s) 46, 214
Fnu4HI GCNGC 2 cut(s) 66, 156
FriOI GRGCYC 4 cut(s) 80, 150, 188, 258
Fsp4HI GCNGC 2 cut(s) 66, 156
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 109
GluI GCNGC 2 cut(s) 66, 156
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 29, 80
HgaI GACGC 1 cut(s) 226
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 2 cut(s) 50, 218
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 1 cut(s) 168
HpaII CCGG 2 cut(s) 29, 80
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 2 cut(s) 165, 173
Hpy188III TCNNGA 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 31, 49
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpyF3I CTNAG 1 cut(s) 69
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 2 cut(s) 50, 218
HspAI GCGC 1 cut(s) 108
Kzo9I GATC 2 cut(s) 94, 173
LmnI GCTCC 5 cut(s) 75, 82, 145, 183, 253
LpnPI CCDG 5 cut(s) 25, 42, 71, 75, 93
Lsp1109I GCAGC 2 cut(s) 77, 167
LweI GCATC 1 cut(s) 211
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 122
MalI GATC 2 cut(s) 96, 175
MboI GATC 2 cut(s) 94, 173
MhlI GDGCHC 4 cut(s) 80, 150, 188, 258
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 4 cut(s) 44, 54, 136, 244
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 2 cut(s) 29, 80
MspR9I CCNGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 203
NciI CCSGG 1 cut(s) 80
NdeII GATC 2 cut(s) 94, 173
NlaIII CATG 2 cut(s) 50, 218
NlaIV GGNNCC 2 cut(s) 60, 77
NmuCI GTSAC 1 cut(s) 122
PagI TCATGA 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PflFI GACNNNGTC 1 cut(s) 236
PinAI ACCGGT 1 cut(s) 28
PkrI GCNGC 2 cut(s) 67, 157
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
PpuMI RGGWCCY 1 cut(s) 58
PsiI TTATAA 1 cut(s) 294
Psp124BI GAGCTC 3 cut(s) 150, 188, 258
Psp5II RGGWCCY 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 60, 77
PspPI GGNCC 3 cut(s) 31, 58, 88
PspPPI RGGWCCY 1 cut(s) 58
PstI CTGCAG 1 cut(s) 67
PsyI GACNNNGTC 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 219
SacI GAGCTC 3 cut(s) 150, 188, 258
SatI GCNGC 2 cut(s) 66, 156
Sau3AI GATC 2 cut(s) 94, 173
Sau96I GGNCC 3 cut(s) 31, 58, 88
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 4 cut(s) 80, 150, 188, 258
SfaNI GCATC 1 cut(s) 211
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 3 cut(s) 31, 58, 88
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 3 cut(s) 150, 188, 258
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 122
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 2 cut(s) 65, 155
Tsp45I GTSAC 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 3 cut(s) 31, 58, 88
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.