pycom08g08350

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000732
Physical Location & Seq
Forward (+)
6710177 .. 6710452
276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08350.1

Sequence Viewer

Length: 276 bp
ATGTCATTTATAAGGAGCCAACCGGCCGGTGTTCCATGGACTAGGGAGCAGGTGTTGAATCTCTTCAATAGTTTTGACAAGGACGGCGATGGCATGCTGTCCAAAGAGGAGGTGAAGGCAGCCTTCAGAAAGCTCGGGTCGCATTGGGGTGGTTTCAGGGCGAGGAGAGCTCTGAGGCATGCAGATGCCAACCGTGACGGGCTCATTTCTGCAGAGGAGATCAATGACCTTGCCAACTATGCCCTAAAATGTCGGTATACGCTAAAACAATTATAG

Protein Analysis

92

Amino Acids

10.36

Weight (kDa)

9.57

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 19 - 46 5.3e-08 EF-hand domain
EF-hand_1 PF00036 20 - 45 7.8e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccI GTMKAC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 109
AgsI TTSAA 2 cut(s) 58, 67
AluBI AGCT 2 cut(s) 133, 170
AluI AGCT 2 cut(s) 133, 170
Alw21I GWGCWC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 134
BanII GRGCYC 2 cut(s) 172, 204
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 1 cut(s) 131
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 100
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 17
BmsI GCATC 1 cut(s) 175
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BsaJI CCNNGG 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bse118I RCCGGY 2 cut(s) 22, 26
BseDI CCNNGG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 164
BseRI GAGGAG 3 cut(s) 122, 178, 230
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 2 cut(s) 23, 27
BsnI GGCC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 172, 204
Bsp143I GATC 1 cut(s) 219
Bsp19I CCATGG 1 cut(s) 35
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 40
BsrFI RCCGGY 2 cut(s) 22, 26
BssAI RCCGGY 2 cut(s) 22, 26
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 1 cut(s) 219
BssNAI GTATAC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 35
Bst1107I GTATAC 1 cut(s) 258
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 95, 180
BstDEI CTNAG 1 cut(s) 173
BstDSI CCRYGG 1 cut(s) 35
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 3 cut(s) 139, 167, 239
BstNSI RCATGY 2 cut(s) 97, 182
BstSFI CTRYAG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 131
BstZ17I GTATAC 1 cut(s) 258
BstZI CGGCCG 1 cut(s) 24
BsuRI GGCC 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 95, 180
Cfr10I RCCGGY 2 cut(s) 22, 26
CviAII CATG 3 cut(s) 36, 94, 179
CviJI RGCY 6 cut(s) 18, 26, 122, 133, 170, 202
CviKI_1 RGCY 6 cut(s) 18, 26, 122, 133, 170, 202
DdeI CTNAG 1 cut(s) 173
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 172, 204
Eco52I CGGCCG 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 35
EcoT38I GRGCYC 2 cut(s) 172, 204
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 3 cut(s) 39, 97, 182
FaiI YATR 7 cut(s) 11, 37, 95, 180, 240, 258, 274
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 3 cut(s) 35, 93, 178
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 120
FriOI GRGCYC 2 cut(s) 172, 204
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 42
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 26
HapII CCGG 2 cut(s) 23, 27
Hin1II CATG 3 cut(s) 39, 97, 182
HinfI GANTC 1 cut(s) 58
HpaII CCGG 2 cut(s) 23, 27
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 2 cut(s) 128, 174
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 109, 133
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4V TGCA 2 cut(s) 182, 212
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 167, 239
HpyF3I CTNAG 1 cut(s) 173
Hsp92II CATG 3 cut(s) 39, 97, 182
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 15, 46
LpnPI CCDG 4 cut(s) 35, 36, 40, 142
Lsp1109I GCAGC 1 cut(s) 131
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 194
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 1 cut(s) 55
MhlI GDGCHC 2 cut(s) 172, 204
MluCI AATT 1 cut(s) 269
MnlI CCTC 5 cut(s) 100, 103, 156, 168, 208
MroXI GAANNNNTTC 1 cut(s) 62
MslI CAYNNNNRTG 2 cut(s) 147, 183
MspI CCGG 2 cut(s) 23, 27
MwoI GCNNNNNNNGC 3 cut(s) 139, 167, 239
NcoI CCATGG 1 cut(s) 35
NdeII GATC 1 cut(s) 219
NlaIII CATG 3 cut(s) 39, 97, 182
NlaIV GGNNCC 1 cut(s) 17
NmuCI GTSAC 1 cut(s) 194
NspI RCATGY 2 cut(s) 97, 182
PaeI GCATGC 2 cut(s) 97, 182
PaqCI CACCTGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 121
PsiI TTATAA 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 214
RseI CAYNNNNRTG 2 cut(s) 147, 183
SacI GAGCTC 1 cut(s) 172
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 219
SduI GDGCHC 2 cut(s) 172, 204
SetI ASST 5 cut(s) 54, 114, 135, 172, 231
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 147, 183
SphI GCATGC 2 cut(s) 97, 182
Sse9I AATT 1 cut(s) 269
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 172
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 194
TasI AATT 1 cut(s) 269
TfiI GAWTC 1 cut(s) 58
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 194
XceI RCATGY 2 cut(s) 97, 182
XmiI GTMKAC 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.