RLG00000010696

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000732
Physical Location & Seq
Forward (+)
3690631 .. 3691194
564 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010696

Sequence Viewer

Length: 366 bp
ATGACCTACTGCCCCGAGCAGAAGGCCATTAAGAAGGTCCCAATTCACTGTATCAGCAAGGAGCAAGTCTGCGATTTTTTCAAAAGATTTGATAGGAACGGTGACGGCAAACTCTGCCAGGAAGAGATCAAGGCAGCCTTTCGGAAACTCGGATCGCGTTGCAGCTCTTACAGAGCCTGGAGAGCATTAGACCATGCAGATTCCAATGGCGATGGCATCATCTCTAATGAGGACCTCGACGACCTTATCAACTATGCCCTAGATTGTGGTTATAAGCTATTAGCGCCACATATATGTCGGAAGGATGGAGGTGACCTCTTGTTGCCCCTCTTTGCCCCGGTGGATGAACAGATTAAAACTGAATAA

Protein Analysis

122

Amino Acids

13.71

Weight (kDa)

5.92

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 25 - 51 5.2e-07 EF-hand domain
EF-hand_1 PF00036 25 - 50 6.8e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AccII CGCG 1 cut(s) 157
AclWI GGATC 1 cut(s) 160
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 2 cut(s) 117, 176
AluBI AGCT 2 cut(s) 165, 277
AluI AGCT 2 cut(s) 165, 277
AlwI GGATC 1 cut(s) 160
AlwNI CAGNNNCTG 1 cut(s) 177
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 134, 162
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 37, 232
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 2 cut(s) 113, 323
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 2 cut(s) 37, 232
BbvI GCAGC 2 cut(s) 146, 174
BccI CCATC 2 cut(s) 206, 299
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 119, 178
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 287
BisI GCNGC 2 cut(s) 135, 163
BlsI GCNGC 2 cut(s) 136, 164
Bme1390I CCNGG 3 cut(s) 119, 178, 338
Bme18I GGWCC 2 cut(s) 37, 232
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 37, 232
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 3 cut(s) 119, 178, 338
BmsI GCATC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 300, 332
BpmI CTGGAG 1 cut(s) 199
BpuMI CCSGG 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 336
BseBI CCWGG 2 cut(s) 119, 178
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 2 cut(s) 310, 349
BseXI GCAGC 2 cut(s) 146, 174
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 14
Bsp143I GATC 2 cut(s) 126, 152
BspANI GGCC 1 cut(s) 26
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 2 cut(s) 126, 152
Bst2UI CCWGG 2 cut(s) 119, 178
Bst4CI ACNGT 2 cut(s) 50, 101
Bst6I CTCTTC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 114
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 2 cut(s) 310, 349
BstFNI CGCG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 2 cut(s) 129, 155
BstMBI GATC 2 cut(s) 126, 152
BstMWI GCNNNNNNNGC 3 cut(s) 114, 182, 283
BstNI CCWGG 2 cut(s) 119, 178
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 3 cut(s) 117, 176, 336
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 146, 174
BsuRI GGCC 1 cut(s) 26
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 2 cut(s) 310, 349
BtsIMutI CAGTG 1 cut(s) 46
CaiI CAGNNNCTG 1 cut(s) 177
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 37, 232
CviAII CATG 1 cut(s) 194
CviJI RGCY 5 cut(s) 26, 137, 165, 176, 277
CviKI_1 RGCY 5 cut(s) 26, 137, 165, 176, 277
DpnI GATC 2 cut(s) 128, 154
DpnII GATC 2 cut(s) 126, 152
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 37, 232
Eco88I CYCGRG 1 cut(s) 14
Eco91I GGTNACC 1 cut(s) 311
EcoO109I RGGNCCY 2 cut(s) 37, 232
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 117, 176
FaeI CATG 1 cut(s) 197
FaiI YATR 6 cut(s) 195, 255, 273, 291, 293, 295
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 1 cut(s) 23
FatI CATG 1 cut(s) 193
Fnu4HI GCNGC 2 cut(s) 135, 163
FokI GGATG 2 cut(s) 317, 356
Fsp4HI GCNGC 2 cut(s) 135, 163
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 285
GluI GCNGC 2 cut(s) 135, 163
GsuI CTGGAG 1 cut(s) 199
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 1 cut(s) 26
HapII CCGG 1 cut(s) 338
HhaI GCGC 1 cut(s) 286
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HinfI GANTC 1 cut(s) 200
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 113, 323
Hpy188I TCNGA 3 cut(s) 144, 152, 300
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 16, 28, 295
HpyCH4III ACNGT 2 cut(s) 50, 101
HpyCH4V TGCA 2 cut(s) 162, 197
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 182, 283
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 2 cut(s) 126, 152
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 5 cut(s) 104, 131, 163, 190, 351
Lsp1109I GCAGC 2 cut(s) 146, 174
LweI GCATC 1 cut(s) 225
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 2 cut(s) 101, 311
MalI GATC 2 cut(s) 128, 154
MboI GATC 2 cut(s) 126, 152
MboII GAAGA 1 cut(s) 134
MluCI AATT 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 278
MnlI CCTC 5 cut(s) 223, 245, 302, 326, 338
MseI TTAA 2 cut(s) 30, 354
MslI CAYNNNNRTG 1 cut(s) 292
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 119, 178, 338
MvaI CCWGG 2 cut(s) 119, 178
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 3 cut(s) 114, 182, 283
NciI CCSGG 1 cut(s) 338
NdeII GATC 2 cut(s) 126, 152
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 101, 311
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 2 cut(s) 136, 164
PpuMI RGGWCCY 2 cut(s) 37, 232
PsiI TTATAA 1 cut(s) 273
Psp5II RGGWCCY 2 cut(s) 37, 232
Psp6I CCWGG 2 cut(s) 117, 176
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 117, 176
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 232
PspPPI RGGWCCY 2 cut(s) 37, 232
PstNI CAGNNNCTG 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 292
SaqAI TTAA 2 cut(s) 30, 354
SatI GCNGC 2 cut(s) 135, 163
Sau3AI GATC 2 cut(s) 126, 152
Sau96I GGNCC 2 cut(s) 37, 232
ScrFI CCNGG 3 cut(s) 119, 178, 338
SetI ASST 8 cut(s) 8, 39, 167, 237, 246, 279, 313, 318
SfaNI GCATC 1 cut(s) 225
SinI GGWCC 2 cut(s) 37, 232
SmiMI CAYNNNNRTG 1 cut(s) 292
Sse9I AATT 1 cut(s) 42
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 3 cut(s) 117, 176, 336
TaaI ACNGT 2 cut(s) 50, 101
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 42
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 2 cut(s) 30, 354
Tru9I TTAA 2 cut(s) 30, 354
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 2 cut(s) 101, 311
TseI GCWGC 2 cut(s) 134, 162
Tsp45I GTSAC 2 cut(s) 101, 311
TspDTI ATGAA 1 cut(s) 360
TspRI CASTG 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 37, 232
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.