Rmu_sc0001772.1_g000027

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001772.1
OG0000732
Physical Location & Seq
Forward (+)
108150 .. 111970
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001772.1_g000027.1.cds

Sequence Viewer

Length: 768 bp
atggatccctgcagtcaaggaacagtgcttacaacagtatttttggggtgccaagaggcaaagaagttgagcaagccatatgttgatcacgtagccattgttgcaaaacctcatcttgaaaaagttaaaagtggttttgaagatctatacaaagcaggtggttcaggcctacgagaaatttctcaaaatccgcttccacaaatcatcatcaaacagtctccgctaagcgcaacacctctagagggacttccccctttcatcttgcaagtgacattctccgctaagtgcaacaccgttagcaggacttctccatttcattttgcagtgacccagtctccgctgaacgcaacaccactcgaggaacttctccacctcgcaagcaagaaggtccccgcgacacttcttaccactttctatcagcgggggacttctgccagcggcgattcccaaggcaacgcctcattcagacaacgaccgccacgtggcattgcagtaaagctacgttcctccgagacaaatcctgatgtttcgtacactgagcagcagatcataaatattttcaagagctttgacctgaacggagacggcaagctgtcctgggatgaggtgaaggctgcgttcgctaaactcggggcgttttgtcctaactacagagcttggcaaggacaaaggcgtgccgatgccgacaaggatggcttcgtctctcttgagaccgagctcgatgaacttgtcacctataccctaaaacaagggtataaaccaaagtaa

Protein Analysis

255

Amino Acids

27.99

Weight (kDa)

8.94

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 48
AccII CGCG 1 cut(s) 395
AciI CCGC 8 cut(s) 191, 221, 279, 338, 393, 421, 438, 476
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 177
AcvI CACGTG 1 cut(s) 482
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 3 cut(s) 242, 300, 482
AgsI TTSAA 3 cut(s) 119, 140, 562
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 5 cut(s) 499, 567, 592, 656, 718
AluI AGCT 5 cut(s) 499, 567, 592, 656, 718
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 6 cut(s) 222, 339, 506, 576, 704, 706
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 2 cut(s) 356, 629
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 2 cut(s) 541, 614
ApoI RAATTY 1 cut(s) 177
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 388
AsuHPI GGTGA 2 cut(s) 619, 724
AvaI CYCGRG 2 cut(s) 356, 629
AvaII GGWCC 1 cut(s) 388
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 720
BbrPI CACGTG 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 2 cut(s) 553, 601
BccI CCATC 1 cut(s) 686
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 1 cut(s) 598
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 6 cut(s) 222, 339, 506, 576, 704, 706
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 2 cut(s) 10, 649
BfuAI ACCTGC 1 cut(s) 146
BglII AGATCT 1 cut(s) 142
BisI GCNGC 3 cut(s) 439, 542, 615
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 3 cut(s) 440, 543, 616
Bme1390I CCNGG 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 388
BmeT110I CYCGRG 2 cut(s) 356, 629
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 3 cut(s) 6, 50, 390
BmrFI CCNGG 1 cut(s) 598
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 1 cut(s) 325
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 728
BsaAI YACGTR 2 cut(s) 91, 482
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 2 cut(s) 448, 597
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 300, 482
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 2 cut(s) 448, 597
BseGI GGATG 2 cut(s) 607, 697
BseLI CCNNNNNNNGG 3 cut(s) 242, 300, 482
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 331
BseXI GCAGC 2 cut(s) 553, 601
Bsh1236I CGCG 1 cut(s) 395
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 48
BsiEI CGRYCG 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 720
BsiHKCI CYCGRG 2 cut(s) 356, 629
BslFI GGGAC 3 cut(s) 258, 374, 439
BslI CCNNNNNNNGG 3 cut(s) 242, 300, 482
BsmAI GTCTC 6 cut(s) 222, 339, 506, 576, 704, 706
BsmBI CGTCTC 2 cut(s) 576, 706
BsmFI GGGAC 3 cut(s) 258, 374, 439
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 704
BsoBI CYCGRG 2 cut(s) 356, 629
Bsp1286I GDGCHC 1 cut(s) 720
Bsp143I GATC 4 cut(s) 4, 85, 142, 546
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 8 cut(s) 191, 221, 279, 338, 393, 421, 438, 476
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 529
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 6, 50, 390
BspMAI CTGCAG 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 448, 597
BssMI GATC 4 cut(s) 4, 85, 142, 546
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 4 cut(s) 25, 37, 216, 295
BstBAI YACGTR 2 cut(s) 91, 482
BstC8I GCNNGC 5 cut(s) 74, 379, 436, 590, 675
BstDEI CTNAG 3 cut(s) 224, 282, 537
BstF5I GGATG 2 cut(s) 607, 697
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 4 cut(s) 7, 88, 145, 549
BstMAI GTCTC 6 cut(s) 222, 339, 506, 576, 704, 706
BstMBI GATC 4 cut(s) 4, 85, 142, 546
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 2 cut(s) 101, 620
BstNI CCWGG 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 596
BstSFI CTRYAG 2 cut(s) 10, 649
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 553, 601
BstX2I RGATCY 2 cut(s) 4, 142
BstYI RGATCY 2 cut(s) 4, 142
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 2 cut(s) 607, 697
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 3 cut(s) 30, 330, 534
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 5 cut(s) 74, 379, 436, 590, 675
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 3 cut(s) 224, 282, 537
DpnI GATC 4 cut(s) 6, 87, 144, 548
DpnII GATC 4 cut(s) 4, 85, 142, 546
Ecl136II GAGCTC 1 cut(s) 718
Eco130I CCWWGG 1 cut(s) 448
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 388
Eco53kI GAGCTC 1 cut(s) 718
Eco72I CACGTG 1 cut(s) 482
Eco88I CYCGRG 2 cut(s) 356, 629
EcoICRI GAGCTC 1 cut(s) 718
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 596
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 448
Esp3I CGTCTC 2 cut(s) 576, 706
FaiI YATR 6 cut(s) 79, 81, 148, 551, 738, 756
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FaqI GGGAC 3 cut(s) 258, 374, 439
FauI CCCGC 2 cut(s) 400, 414
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 439, 542, 615
FokI GGATG 2 cut(s) 614, 704
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 3 cut(s) 439, 542, 615
FspBI CTAG 1 cut(s) 239
GlaI GCGC 1 cut(s) 229
GluI GCNGC 3 cut(s) 439, 542, 615
HaeIII GGCC 1 cut(s) 168
HhaI GCGC 1 cut(s) 230
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HinfI GANTC 1 cut(s) 443
HphI GGTGA 2 cut(s) 619, 724
Hpy166II GTNNAC 1 cut(s) 534
Hpy188I TCNGA 2 cut(s) 467, 511
Hpy188III TCNNGA 5 cut(s) 116, 239, 521, 562, 707
Hpy8I GTNNAC 1 cut(s) 534
HpyAV CCTTC 2 cut(s) 379, 604
HpyCH4III ACNGT 4 cut(s) 25, 37, 216, 295
HpyCH4IV ACGT 3 cut(s) 90, 481, 502
HpyCH4V TGCA 6 cut(s) 12, 104, 265, 288, 323, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 620
HpyF3I CTNAG 3 cut(s) 224, 282, 537
HpySE526I ACGT 3 cut(s) 90, 481, 502
HspAI GCGC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 4 cut(s) 4, 85, 142, 546
Lsp1109I GCAGC 2 cut(s) 553, 601
LweI GCATC 1 cut(s) 670
MaeI CTAG 1 cut(s) 239
MaeII ACGT 3 cut(s) 90, 481, 502
MaeIII GTNAC 3 cut(s) 268, 325, 730
MalI GATC 4 cut(s) 6, 87, 144, 548
MboI GATC 4 cut(s) 4, 85, 142, 546
MboII GAAGA 1 cut(s) 152
MflI RGATCY 2 cut(s) 4, 142
MhlI GDGCHC 1 cut(s) 720
MluCI AATT 1 cut(s) 177
MnlI CCTC 9 cut(s) 49, 120, 235, 246, 352, 383, 469, 517, 598
MseI TTAA 1 cut(s) 126
MspA1I CMGCKG 3 cut(s) 340, 421, 438
MspR9I CCNGG 1 cut(s) 598
MvaI CCWGG 1 cut(s) 598
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 101, 620
NdeI CATATG 1 cut(s) 79
NdeII GATC 4 cut(s) 4, 85, 142, 546
NlaIV GGNNCC 3 cut(s) 6, 50, 390
NmuCI GTSAC 3 cut(s) 268, 325, 730
PaeR7I CTCGAG 1 cut(s) 356
PaqCI CACCTGC 1 cut(s) 146
PceI AGGCCT 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 478
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 3 cut(s) 440, 543, 616
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
Ppu21I YACGTR 2 cut(s) 91, 482
PpuMI RGGWCCY 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 596
PspCI CACGTG 1 cut(s) 482
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 3 cut(s) 6, 50, 390
PspPI GGNCC 1 cut(s) 388
PspPPI RGGWCCY 1 cut(s) 388
PspXI VCTCGAGB 1 cut(s) 356
PsrI GAACNNNNNNTAC 2 cut(s) 13, 45
PstI CTGCAG 1 cut(s) 14
PsuI RGATCY 2 cut(s) 4, 142
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
SacI GAGCTC 1 cut(s) 720
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 3 cut(s) 439, 542, 615
Sau3AI GATC 4 cut(s) 4, 85, 142, 546
Sau96I GGNCC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 598
SduI GDGCHC 1 cut(s) 720
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 2 cut(s) 10, 649
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 1 cut(s) 388
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 2 cut(s) 356, 707
SmoI CTYRAG 2 cut(s) 356, 707
Sse9I AATT 1 cut(s) 177
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 8 cut(s) 191, 221, 279, 338, 393, 421, 438, 476
SspI AATATT 1 cut(s) 556
SspMI CTAG 1 cut(s) 239
SstI GAGCTC 1 cut(s) 720
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 596
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 4 cut(s) 25, 37, 216, 295
TaiI ACGT 3 cut(s) 93, 484, 505
TaqI TCGA 2 cut(s) 357, 720
TaqII GACCGA 1 cut(s) 728
TasI AATT 1 cut(s) 177
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 3 cut(s) 30, 330, 541
TseFI GTSAC 3 cut(s) 268, 325, 730
TseI GCWGC 2 cut(s) 541, 614
Tsp45I GTSAC 3 cut(s) 268, 325, 730
TspDTI ATGAA 3 cut(s) 247, 305, 738
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 3 cut(s) 30, 330, 541
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 238
XhoI CTCGAG 1 cut(s) 356
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.