RLG00000010698

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000732
Physical Location & Seq
Forward (+)
3704809 .. 3706182
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010698

Sequence Viewer

Length: 552 bp
ATGGTGGAGGAGAAGATTATTGGATCTGGGCATGCAAATTGGGGTGATGGTAGATTTTGGCGTGAATTTGGTGGAGGTGGTCAATTGCAAATGGGGTATGGTTCGCCAATGTGGTTCAGAATTGGCGACGCATTAACATATCGCAGAGCTGCATATCATGGCTTGGTTGACTGGTGGAATTTCTCCTCTTCTAGGCGAGAAATTTATAGGGTTGTCCATCTTCCACCTTCTAAACAAGAACTAGAAAGTTCTGCTTCTTGTTTTGCAGCGGGCATGACCTACTGGCCTGAGCAGACGGCCATTAAGAAGGTCTCAATTCACTGGAGCAAGGAGCAAGTCCACGATCTTTTCAAAAGCTTTGATAGGAACGGTGACGGCAAACTCTCCAAGGAAGAGCTCAAGGCAGCATTTCGGAAACTCGGATCTCGTTGGAGCTCTTACAGAGCTAGGAGAGCATTACGCCATGCAGATTCCAATGGCGACAGCATCATCTCTAATGAGGAGCTCGACGATCTTATAAACTATGCCCTAAAATGTGGTTATAAGCTATAA

Protein Analysis

184

Amino Acids

21.02

Weight (kDa)

9.2

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 100 - 138 2.2e-06 EF-hand domain pair
EF-hand_7 PF13499 111 - 140 2.6e-06 EF-hand domain pair
EF-hand_6 PF13405 113 - 141 1.2e-08 EF-hand domain
EF-hand_1 PF00036 113 - 140 1.4e-07 EF hand domain
EF-hand_5 PF13202 115 - 135 1.3e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 518, 543
AciI CCGC 1 cut(s) 269
AclWI GGATC 2 cut(s) 31, 430
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 3 cut(s) 65, 178, 201
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 1 cut(s) 352
AluBI AGCT 7 cut(s) 149, 357, 397, 435, 446, 505, 547
AluI AGCT 7 cut(s) 149, 357, 397, 435, 446, 505, 547
Alw21I GWGCWC 3 cut(s) 399, 437, 507
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 2 cut(s) 31, 430
AoxI GGCC 2 cut(s) 284, 297
ApeKI GCWGC 3 cut(s) 149, 266, 404
ApoI RAATTY 3 cut(s) 65, 178, 201
AsuHPI GGTGA 2 cut(s) 56, 383
BanII GRGCYC 3 cut(s) 399, 437, 507
Bbv12I GWGCWC 3 cut(s) 399, 437, 507
BbvI GCAGC 3 cut(s) 136, 278, 416
BccI CCATC 2 cut(s) 41, 225
BceAI ACGGC 2 cut(s) 312, 391
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 3 cut(s) 192, 242, 447
BisI GCNGC 3 cut(s) 150, 267, 405
BlsI GCNGC 3 cut(s) 151, 268, 406
BmsI GCATC 1 cut(s) 495
BpmI CTGGAG 1 cut(s) 343
Bpu10I CCTNAGC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 3 cut(s) 176, 287, 326
BseDI CCNNGG 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 3 cut(s) 176, 287, 326
BseRI GAGGAG 3 cut(s) 23, 175, 515
BseXI GCAGC 3 cut(s) 136, 278, 416
BshFI GGCC 2 cut(s) 286, 299
BsiHKAI GWGCWC 3 cut(s) 399, 437, 507
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 2 cut(s) 286, 299
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 3 cut(s) 399, 437, 507
Bsp143I GATC 4 cut(s) 23, 343, 422, 511
BspACI CCGC 1 cut(s) 269
BspANI GGCC 2 cut(s) 286, 299
BspCNI CTCAG 1 cut(s) 280
BspPI GGATC 2 cut(s) 31, 430
BspQI GCTCTTC 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 316
BsrI ACTGG 3 cut(s) 176, 287, 326
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 4 cut(s) 23, 343, 422, 511
BssT1I CCWWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 2 cut(s) 193, 387
BstC8I GCNNGC 2 cut(s) 33, 271
BstDEI CTNAG 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 190
BstKTI GATC 4 cut(s) 26, 346, 425, 514
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 4 cut(s) 23, 343, 422, 511
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNSI RCATGY 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 136, 278, 416
BstX2I RGATCY 2 cut(s) 23, 422
BstYI RGATCY 2 cut(s) 23, 422
BsuRI GGCC 2 cut(s) 286, 299
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 2 cut(s) 33, 271
CseI GACGC 1 cut(s) 137
CviAII CATG 4 cut(s) 32, 158, 274, 464
DdeI CTNAG 1 cut(s) 288
DpnI GATC 4 cut(s) 25, 345, 424, 513
DpnII GATC 4 cut(s) 23, 343, 422, 511
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 193, 387
EarI CTCTTC 2 cut(s) 193, 387
Ecl136II GAGCTC 3 cut(s) 397, 435, 505
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 3 cut(s) 399, 437, 507
Eco31I GGTCTC 1 cut(s) 316
Eco53kI GAGCTC 3 cut(s) 397, 435, 505
EcoICRI GAGCTC 3 cut(s) 397, 435, 505
EcoNI CCTNNNNNAGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 3 cut(s) 399, 437, 507
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 35, 161, 277, 467
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FatI CATG 4 cut(s) 31, 157, 273, 463
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 3 cut(s) 150, 267, 405
FriOI GRGCYC 3 cut(s) 399, 437, 507
Fsp4HI GCNGC 3 cut(s) 150, 267, 405
FspBI CTAG 3 cut(s) 192, 242, 447
GluI GCNGC 3 cut(s) 150, 267, 405
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 2 cut(s) 286, 299
HgaI GACGC 1 cut(s) 137
Hin1II CATG 4 cut(s) 35, 161, 277, 467
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 1 cut(s) 470
HphI GGTGA 2 cut(s) 56, 383
Hpy166II GTNNAC 2 cut(s) 169, 340
Hpy188I TCNGA 3 cut(s) 119, 414, 422
Hpy8I GTNNAC 2 cut(s) 169, 340
Hpy99I CGWCG 2 cut(s) 131, 512
HpyAV CCTTC 2 cut(s) 237, 301
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 5 cut(s) 35, 88, 152, 266, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 4 cut(s) 35, 161, 277, 467
Kzo9I GATC 4 cut(s) 23, 343, 422, 511
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 4 cut(s) 324, 331, 432, 502
LpnPI CCDG 5 cut(s) 12, 157, 268, 300, 307
Lsp1109I GCAGC 3 cut(s) 136, 278, 416
LweI GCATC 1 cut(s) 495
MaeI CTAG 3 cut(s) 192, 242, 447
MaeIII GTNAC 1 cut(s) 371
MalI GATC 4 cut(s) 25, 345, 424, 513
MboI GATC 4 cut(s) 23, 343, 422, 511
MboII GAAGA 4 cut(s) 25, 180, 212, 404
MfeI CAATTG 1 cut(s) 83
MflI RGATCY 2 cut(s) 23, 422
MhlI GDGCHC 3 cut(s) 399, 437, 507
MluCI AATT 7 cut(s) 37, 65, 83, 120, 178, 201, 315
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 3 cut(s) 68, 196, 493
MseI TTAA 2 cut(s) 134, 303
MspA1I CMGCKG 1 cut(s) 269
MunI CAATTG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 452
NdeII GATC 4 cut(s) 23, 343, 422, 511
NlaIII CATG 4 cut(s) 35, 161, 277, 467
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 1 cut(s) 35
PaeI GCATGC 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 3 cut(s) 151, 268, 406
PsiI TTATAA 2 cut(s) 518, 543
Psp124BI GAGCTC 3 cut(s) 399, 437, 507
PsuI RGATCY 2 cut(s) 23, 422
SacI GAGCTC 3 cut(s) 399, 437, 507
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 134, 303
SatI GCNGC 3 cut(s) 150, 267, 405
Sau3AI GATC 4 cut(s) 23, 343, 422, 511
SduI GDGCHC 3 cut(s) 399, 437, 507
SfaNI GCATC 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SphI GCATGC 1 cut(s) 35
Sse9I AATT 7 cut(s) 37, 65, 83, 120, 178, 201, 315
SsiI CCGC 1 cut(s) 269
SspMI CTAG 3 cut(s) 192, 242, 447
SstI GAGCTC 3 cut(s) 399, 437, 507
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 507
TasI AATT 7 cut(s) 37, 65, 83, 120, 178, 201, 315
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 134, 303
Tru9I TTAA 2 cut(s) 134, 303
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 3 cut(s) 149, 266, 404
Tsp45I GTSAC 1 cut(s) 371
TspRI CASTG 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 190
XapI RAATTY 3 cut(s) 65, 178, 201
XceI RCATGY 1 cut(s) 35
XspI CTAG 3 cut(s) 192, 242, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.