calcium ion binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 36 |
| AfaI | GTAC | 2 cut(s) | 6, 38 |
| AgsI | TTSAA | 1 cut(s) | 67 |
| AjnI | CCWGG | 1 cut(s) | 101 |
| AluBI | AGCT | 3 cut(s) | 161, 177, 223 |
| AluI | AGCT | 3 cut(s) | 161, 177, 223 |
| Alw21I | GWGCWC | 1 cut(s) | 225 |
| Alw26I | GTCTC | 2 cut(s) | 81, 209 |
| AlwI | GGATC | 1 cut(s) | 36 |
| Ama87I | CYCGRG | 2 cut(s) | 134, 212 |
| ApeKI | GCWGC | 2 cut(s) | 46, 119 |
| AsuHPI | GGTGA | 1 cut(s) | 124 |
| AvaI | CYCGRG | 2 cut(s) | 134, 212 |
| BaeGI | GKGCMC | 1 cut(s) | 17 |
| BanII | GRGCYC | 1 cut(s) | 225 |
| Bbv12I | GWGCWC | 1 cut(s) | 225 |
| BbvI | GCAGC | 2 cut(s) | 58, 106 |
| BccI | CCATC | 1 cut(s) | 191 |
| BceAI | ACGGC | 1 cut(s) | 106 |
| BciT130I | CCWGG | 1 cut(s) | 103 |
| BcoDI | GTCTC | 2 cut(s) | 81, 209 |
| BfmI | CTRYAG | 2 cut(s) | 154, 254 |
| BisI | GCNGC | 2 cut(s) | 47, 120 |
| BlsI | GCNGC | 2 cut(s) | 48, 121 |
| Bme1390I | CCNGG | 1 cut(s) | 103 |
| BmeT110I | CYCGRG | 2 cut(s) | 134, 212 |
| BmrFI | CCNGG | 1 cut(s) | 103 |
| BmsI | GCATC | 1 cut(s) | 175 |
| BsaAI | YACGTR | 1 cut(s) | 8 |
| BsaI | GGTCTC | 1 cut(s) | 209 |
| BsaJI | CCNNGG | 1 cut(s) | 102 |
| BseBI | CCWGG | 1 cut(s) | 103 |
| BseDI | CCNNGG | 1 cut(s) | 102 |
| BseGI | GGATG | 2 cut(s) | 112, 202 |
| BseMII | CTCAG | 1 cut(s) | 33 |
| BseSI | GKGCMC | 1 cut(s) | 17 |
| BseXI | GCAGC | 2 cut(s) | 58, 106 |
| BsiHKAI | GWGCWC | 1 cut(s) | 225 |
| BsiHKCI | CYCGRG | 2 cut(s) | 134, 212 |
| BsmAI | GTCTC | 2 cut(s) | 81, 209 |
| BsmBI | CGTCTC | 1 cut(s) | 81 |
| Bso31I | GGTCTC | 1 cut(s) | 209 |
| BsoBI | CYCGRG | 2 cut(s) | 134, 212 |
| Bsp1286I | GDGCHC | 2 cut(s) | 17, 225 |
| Bsp143I | GATC | 2 cut(s) | 28, 51 |
| BspCNI | CTCAG | 1 cut(s) | 34 |
| BspPI | GGATC | 1 cut(s) | 36 |
| BspTNI | GGTCTC | 1 cut(s) | 209 |
| BssECI | CCNNGG | 1 cut(s) | 102 |
| BssMI | GATC | 2 cut(s) | 28, 51 |
| Bst2UI | CCWGG | 1 cut(s) | 103 |
| Bst4CI | ACNGT | 3 cut(s) | 19, 99, 258 |
| BstBAI | YACGTR | 1 cut(s) | 8 |
| BstDEI | CTNAG | 1 cut(s) | 42 |
| BstF5I | GGATG | 2 cut(s) | 112, 202 |
| BstKTI | GATC | 2 cut(s) | 31, 54 |
| BstMAI | GTCTC | 2 cut(s) | 81, 209 |
| BstMBI | GATC | 2 cut(s) | 28, 51 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 125 |
| BstNI | CCWGG | 1 cut(s) | 103 |
| BstSCI | CCNGG | 1 cut(s) | 101 |
| BstSFI | CTRYAG | 2 cut(s) | 154, 254 |
| BstSLI | GKGCMC | 1 cut(s) | 17 |
| BstV1I | GCAGC | 2 cut(s) | 58, 106 |
| BstX2I | RGATCY | 1 cut(s) | 28 |
| BstYI | RGATCY | 1 cut(s) | 28 |
| BtsCI | GGATG | 2 cut(s) | 112, 202 |
| BtsIMutI | CAGTG | 2 cut(s) | 15, 39 |
| Csp6I | GTAC | 2 cut(s) | 5, 37 |
| CviJI | RGCY | 5 cut(s) | 119, 161, 177, 201, 223 |
| CviKI_1 | RGCY | 5 cut(s) | 119, 161, 177, 201, 223 |
| CviQI | GTAC | 2 cut(s) | 5, 37 |
| DdeI | CTNAG | 1 cut(s) | 42 |
| DpnI | GATC | 2 cut(s) | 30, 53 |
| DpnII | GATC | 2 cut(s) | 28, 51 |
| Ecl136II | GAGCTC | 1 cut(s) | 223 |
| Eco24I | GRGCYC | 1 cut(s) | 225 |
| Eco31I | GGTCTC | 1 cut(s) | 209 |
| Eco53kI | GAGCTC | 1 cut(s) | 223 |
| Eco88I | CYCGRG | 2 cut(s) | 134, 212 |
| EcoICRI | GAGCTC | 1 cut(s) | 223 |
| EcoRII | CCWGG | 1 cut(s) | 101 |
| EcoT38I | GRGCYC | 1 cut(s) | 225 |
| Esp3I | CGTCTC | 1 cut(s) | 81 |
| FaiI | YATR | 2 cut(s) | 243, 261 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 185, 217 |
| Fnu4HI | GCNGC | 2 cut(s) | 47, 120 |
| FokI | GGATG | 2 cut(s) | 119, 209 |
| FriOI | GRGCYC | 1 cut(s) | 225 |
| Fsp4HI | GCNGC | 2 cut(s) | 47, 120 |
| GluI | GCNGC | 2 cut(s) | 47, 120 |
| HphI | GGTGA | 1 cut(s) | 124 |
| Hpy166II | GTNNAC | 1 cut(s) | 39 |
| Hpy188I | TCNGA | 1 cut(s) | 56 |
| Hpy188III | TCNNGA | 2 cut(s) | 212, 214 |
| Hpy8I | GTNNAC | 1 cut(s) | 39 |
| HpyAV | CCTTC | 1 cut(s) | 109 |
| HpyCH4III | ACNGT | 3 cut(s) | 19, 99, 258 |
| HpyCH4IV | ACGT | 1 cut(s) | 7 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 125 |
| HpyF3I | CTNAG | 1 cut(s) | 42 |
| HpySE526I | ACGT | 1 cut(s) | 7 |
| Kzo9I | GATC | 2 cut(s) | 28, 51 |
| LpnPI | CCDG | 3 cut(s) | 88, 92, 115 |
| Lsp1109I | GCAGC | 2 cut(s) | 58, 106 |
| LweI | GCATC | 1 cut(s) | 175 |
| MaeII | ACGT | 1 cut(s) | 7 |
| MalI | GATC | 2 cut(s) | 30, 53 |
| MboI | GATC | 2 cut(s) | 28, 51 |
| MboII | GAAGA | 1 cut(s) | 23 |
| MflI | RGATCY | 1 cut(s) | 28 |
| MhlI | GDGCHC | 2 cut(s) | 17, 225 |
| MnlI | CCTC | 3 cut(s) | 4, 103, 161 |
| MseI | TTAA | 1 cut(s) | 24 |
| MspR9I | CCNGG | 1 cut(s) | 103 |
| MvaI | CCWGG | 1 cut(s) | 103 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 125 |
| NdeII | GATC | 2 cut(s) | 28, 51 |
| PaeR7I | CTCGAG | 1 cut(s) | 212 |
| PkrI | GCNGC | 2 cut(s) | 48, 121 |
| Ppu21I | YACGTR | 1 cut(s) | 8 |
| Psp124BI | GAGCTC | 1 cut(s) | 225 |
| Psp6I | CCWGG | 1 cut(s) | 101 |
| PspGI | CCWGG | 1 cut(s) | 101 |
| PsuI | RGATCY | 1 cut(s) | 28 |
| RsaI | GTAC | 2 cut(s) | 6, 38 |
| RsaNI | GTAC | 2 cut(s) | 5, 37 |
| SacI | GAGCTC | 1 cut(s) | 225 |
| SaqAI | TTAA | 1 cut(s) | 24 |
| SatI | GCNGC | 2 cut(s) | 47, 120 |
| Sau3AI | GATC | 2 cut(s) | 28, 51 |
| ScrFI | CCNGG | 1 cut(s) | 103 |
| SduI | GDGCHC | 2 cut(s) | 17, 225 |
| SetI | ASST | 7 cut(s) | 10, 73, 81, 114, 163, 179, 225 |
| SfaNI | GCATC | 1 cut(s) | 175 |
| SfcI | CTRYAG | 2 cut(s) | 154, 254 |
| Sfr274I | CTCGAG | 1 cut(s) | 212 |
| SlaI | CTCGAG | 1 cut(s) | 212 |
| SmlI | CTYRAG | 1 cut(s) | 212 |
| SmoI | CTYRAG | 1 cut(s) | 212 |
| SspI | AATATT | 1 cut(s) | 61 |
| SstI | GAGCTC | 1 cut(s) | 225 |
| StyD4I | CCNGG | 1 cut(s) | 101 |
| TaaI | ACNGT | 3 cut(s) | 19, 99, 258 |
| TaiI | ACGT | 1 cut(s) | 10 |
| TaqI | TCGA | 1 cut(s) | 213 |
| TaqII | GACCGA | 1 cut(s) | 233 |
| Tru1I | TTAA | 1 cut(s) | 24 |
| Tru9I | TTAA | 1 cut(s) | 24 |
| TscAI | CASTG | 2 cut(s) | 22, 46 |
| TseI | GCWGC | 2 cut(s) | 46, 119 |
| TspDTI | ATGAA | 2 cut(s) | 193, 243 |
| TspGWI | ACGGA | 1 cut(s) | 24 |
| TspRI | CASTG | 2 cut(s) | 22, 46 |
| XhoI | CTCGAG | 1 cut(s) | 212 |