Rh6BG229500

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000732
Physical Location & Seq
Forward (+)
43331925 .. 43332847
923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG229500.1

Sequence Viewer

Length: 312 bp
ATGGTGCGTGAGGCACTGAAAGACCGTAAGATGGTGCATGAGGTCATTGCGTCTAAGGGTCTTCCGTACACTGAGCAGCAGATCAGAAATATTTTCAAAGGTTTTGACCTGAATGGAGACGGCAAACTGTCCTGGGATGAGGTGAAGGCTGCGTTCGCTAAACTCGGGGCGTTTTTGCCCGACTACAGAGCTTGGCGAGGACGAAGCTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCACAAACTACAGTATAAACCGAATGGAAGTAATTAA

Protein Analysis

103

Amino Acids

11.72

Weight (kDa)

7.83

Isoelectric Point (pI)

22.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 15 - 52 1.1e-06 EF-hand domain pair
EF-hand_8 PF13833 20 - 49 8.2e-07 EF-hand domain pair
EF-hand_6 PF13405 27 - 56 3.2e-07 EF-hand domain
EF-hand_1 PF00036 28 - 55 4.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 3 cut(s) 191, 207, 253
AluI AGCT 3 cut(s) 191, 207, 253
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 111, 239
Ama87I CYCGRG 2 cut(s) 164, 242
ApeKI GCWGC 2 cut(s) 76, 149
AsuHPI GGTGA 2 cut(s) 154, 259
AvaI CYCGRG 2 cut(s) 164, 242
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 88, 136
BccI CCATC 2 cut(s) 25, 221
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 111, 239
BfmI CTRYAG 2 cut(s) 184, 284
BisI GCNGC 2 cut(s) 77, 150
BlsI GCNGC 2 cut(s) 78, 151
Bme1390I CCNGG 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 164, 242
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 142, 232
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 63
BseXI GCAGC 2 cut(s) 88, 136
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 2 cut(s) 164, 242
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 111, 239
BsmBI CGTCTC 1 cut(s) 111
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 2 cut(s) 164, 242
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 3 cut(s) 26, 129, 288
BstDEI CTNAG 2 cut(s) 54, 72
BstF5I GGATG 2 cut(s) 142, 232
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 111, 239
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 2 cut(s) 184, 284
BstV1I GCAGC 2 cut(s) 88, 136
BstV2I GAAGAC 1 cut(s) 53
BtsCI GGATG 2 cut(s) 142, 232
BtsIMutI CAGTG 2 cut(s) 14, 69
CseI GACGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 67
CviAII CATG 1 cut(s) 38
CviJI RGCY 5 cut(s) 149, 191, 207, 231, 253
CviKI_1 RGCY 5 cut(s) 149, 191, 207, 231, 253
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 2 cut(s) 54, 72
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 2 cut(s) 164, 242
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 1 cut(s) 41
FaiI YATR 3 cut(s) 39, 273, 291
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 77, 150
FokI GGATG 2 cut(s) 149, 239
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 2 cut(s) 77, 150
GluI GCNGC 2 cut(s) 77, 150
HgaI GACGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 41
HphI GGTGA 2 cut(s) 154, 259
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 2 cut(s) 242, 244
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 3 cut(s) 26, 129, 288
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 54, 72
Hsp92II CATG 1 cut(s) 41
Kzo9I GATC 1 cut(s) 81
LpnPI CCDG 3 cut(s) 118, 122, 145
Lsp1109I GCAGC 2 cut(s) 88, 136
LweI GCATC 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MboII GAAGA 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 1 cut(s) 307
MnlI CCTC 4 cut(s) 4, 34, 133, 191
MseI TTAA 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 1 cut(s) 81
NlaIII CATG 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 242
PkrI GCNGC 2 cut(s) 78, 151
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 310
SatI GCNGC 2 cut(s) 77, 150
Sau3AI GATC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 45, 103, 111, 144, 193, 209, 255, 272
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 2 cut(s) 184, 284
Sfr274I CTCGAG 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 1 cut(s) 307
SspI AATATT 1 cut(s) 91
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 131
TaaI ACNGT 3 cut(s) 26, 129, 288
TaqI TCGA 1 cut(s) 243
TaqII GACCGA 1 cut(s) 263
TasI AATT 1 cut(s) 307
Tru1I TTAA 1 cut(s) 310
Tru9I TTAA 1 cut(s) 310
TscAI CASTG 2 cut(s) 21, 76
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 76, 149
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 223, 273
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 2 cut(s) 21, 76
XhoI CTCGAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.