calcium ion binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 709 |
| AccBSI | CCGCTC | 1 cut(s) | 464 |
| AciI | CCGC | 3 cut(s) | 462, 501, 687 |
| AclWI | GGATC | 3 cut(s) | 377, 437, 656 |
| AfaI | GTAC | 1 cut(s) | 68 |
| AflIII | ACRYGT | 1 cut(s) | 583 |
| AgsI | TTSAA | 2 cut(s) | 97, 404 |
| AjnI | CCWGG | 2 cut(s) | 131, 386 |
| AluBI | AGCT | 7 cut(s) | 102, 127, 191, 253, 362, 410, 669 |
| AluI | AGCT | 7 cut(s) | 102, 127, 191, 253, 362, 410, 669 |
| Alw21I | GWGCWC | 1 cut(s) | 255 |
| Alw26I | GTCTC | 3 cut(s) | 111, 239, 241 |
| AlwI | GGATC | 3 cut(s) | 377, 437, 656 |
| Ama87I | CYCGRG | 1 cut(s) | 164 |
| AoxI | GGCC | 2 cut(s) | 552, 642 |
| ApeKI | GCWGC | 2 cut(s) | 76, 149 |
| AspS9I | GGNCC | 2 cut(s) | 53, 643 |
| AsuHPI | GGTGA | 8 cut(s) | 50, 154, 259, 451, 458, 505, 511, 520 |
| AvaI | CYCGRG | 1 cut(s) | 164 |
| AvaII | GGWCC | 1 cut(s) | 53 |
| BanII | GRGCYC | 1 cut(s) | 255 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 430, 462 |
| BbsI | GAAGAC | 1 cut(s) | 332 |
| Bbv12I | GWGCWC | 1 cut(s) | 255 |
| BbvCI | CCTCAGC | 1 cut(s) | 633 |
| BbvI | GCAGC | 2 cut(s) | 88, 136 |
| BccI | CCATC | 4 cut(s) | 221, 385, 475, 539 |
| BceAI | ACGGC | 2 cut(s) | 136, 592 |
| BciT130I | CCWGG | 2 cut(s) | 133, 388 |
| BcoDI | GTCTC | 3 cut(s) | 111, 239, 241 |
| BfmI | CTRYAG | 1 cut(s) | 184 |
| BisI | GCNGC | 3 cut(s) | 77, 150, 501 |
| BlsI | GCNGC | 3 cut(s) | 78, 151, 502 |
| Bme1390I | CCNGG | 2 cut(s) | 133, 388 |
| Bme18I | GGWCC | 1 cut(s) | 53 |
| BmeT110I | CYCGRG | 1 cut(s) | 164 |
| BmgT120I | GGNCC | 2 cut(s) | 53, 643 |
| BmiI | GGNNCC | 1 cut(s) | 374 |
| BmrFI | CCNGG | 2 cut(s) | 133, 388 |
| BmsI | GCATC | 2 cut(s) | 205, 337 |
| BpiI | GAAGAC | 1 cut(s) | 332 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 417, 449 |
| Bpu10I | CCTNAGC | 1 cut(s) | 633 |
| BpuEI | CTTGAG | 1 cut(s) | 263 |
| BsaI | GGTCTC | 1 cut(s) | 239 |
| BsaJI | CCNNGG | 6 cut(s) | 5, 132, 386, 387, 555, 612 |
| BseBI | CCWGG | 2 cut(s) | 133, 388 |
| BseDI | CCNNGG | 6 cut(s) | 5, 132, 386, 387, 555, 612 |
| BseGI | GGATG | 3 cut(s) | 142, 232, 397 |
| BseMII | CTCAG | 3 cut(s) | 63, 359, 624 |
| BseRI | GAGGAG | 1 cut(s) | 673 |
| BseXI | GCAGC | 2 cut(s) | 88, 136 |
| BshFI | GGCC | 2 cut(s) | 554, 644 |
| BsiHKAI | GWGCWC | 1 cut(s) | 255 |
| BsiHKCI | CYCGRG | 1 cut(s) | 164 |
| BsmAI | GTCTC | 3 cut(s) | 111, 239, 241 |
| BsmBI | CGTCTC | 2 cut(s) | 111, 241 |
| BsmI | GAATGC | 1 cut(s) | 435 |
| BsnI | GGCC | 2 cut(s) | 554, 644 |
| Bso31I | GGTCTC | 1 cut(s) | 239 |
| BsoBI | CYCGRG | 1 cut(s) | 164 |
| Bsp1286I | GDGCHC | 1 cut(s) | 255 |
| Bsp143I | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| BspACI | CCGC | 3 cut(s) | 462, 501, 687 |
| BspANI | GGCC | 2 cut(s) | 554, 644 |
| BspCNI | CTCAG | 3 cut(s) | 64, 360, 625 |
| BspLI | GGNNCC | 1 cut(s) | 374 |
| BspPI | GGATC | 3 cut(s) | 377, 437, 656 |
| BspTNI | GGTCTC | 1 cut(s) | 239 |
| BsrBI | CCGCTC | 1 cut(s) | 464 |
| BssECI | CCNNGG | 6 cut(s) | 5, 132, 386, 387, 555, 612 |
| BssMI | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| BssT1I | CCWWGG | 2 cut(s) | 555, 612 |
| Bst2UI | CCWGG | 2 cut(s) | 133, 388 |
| Bst4CI | ACNGT | 1 cut(s) | 706 |
| BstC8I | GCNNGC | 2 cut(s) | 125, 210 |
| BstDEI | CTNAG | 4 cut(s) | 72, 368, 549, 633 |
| BstF5I | GGATG | 3 cut(s) | 142, 232, 397 |
| BstKTI | GATC | 5 cut(s) | 84, 367, 385, 445, 664 |
| BstMAI | GTCTC | 3 cut(s) | 111, 239, 241 |
| BstMBI | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 155 |
| BstNI | CCWGG | 2 cut(s) | 133, 388 |
| BstNSI | RCATGY | 1 cut(s) | 587 |
| BstSCI | CCNGG | 2 cut(s) | 131, 386 |
| BstSFI | CTRYAG | 1 cut(s) | 184 |
| BstV1I | GCAGC | 2 cut(s) | 88, 136 |
| BstV2I | GAAGAC | 1 cut(s) | 332 |
| BstX2I | RGATCY | 2 cut(s) | 442, 661 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 318 |
| BstYI | RGATCY | 2 cut(s) | 442, 661 |
| BsuRI | GGCC | 2 cut(s) | 554, 644 |
| BtsCI | GGATG | 3 cut(s) | 142, 232, 397 |
| BtsIMutI | CAGTG | 1 cut(s) | 69 |
| Cac8I | GCNNGC | 2 cut(s) | 125, 210 |
| Cfr13I | GGNCC | 2 cut(s) | 53, 643 |
| Csp6I | GTAC | 1 cut(s) | 67 |
| CviAII | CATG | 3 cut(s) | 584, 653, 700 |
| CviQI | GTAC | 1 cut(s) | 67 |
| DdeI | CTNAG | 4 cut(s) | 72, 368, 549, 633 |
| DpnI | GATC | 5 cut(s) | 83, 366, 384, 444, 663 |
| DpnII | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 709 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 709 |
| EciI | GGCGGA | 1 cut(s) | 676 |
| Ecl136II | GAGCTC | 1 cut(s) | 253 |
| Eco130I | CCWWGG | 2 cut(s) | 555, 612 |
| Eco24I | GRGCYC | 1 cut(s) | 255 |
| Eco31I | GGTCTC | 1 cut(s) | 239 |
| Eco47I | GGWCC | 1 cut(s) | 53 |
| Eco53kI | GAGCTC | 1 cut(s) | 253 |
| Eco88I | CYCGRG | 1 cut(s) | 164 |
| EcoICRI | GAGCTC | 1 cut(s) | 253 |
| EcoO109I | RGGNCCY | 1 cut(s) | 53 |
| EcoRII | CCWGG | 2 cut(s) | 131, 386 |
| EcoT14I | CCWWGG | 2 cut(s) | 555, 612 |
| EcoT38I | GRGCYC | 1 cut(s) | 255 |
| ErhI | CCWWGG | 2 cut(s) | 555, 612 |
| Esp3I | CGTCTC | 2 cut(s) | 111, 241 |
| FaeI | CATG | 3 cut(s) | 587, 656, 703 |
| FaiI | YATR | 7 cut(s) | 48, 86, 273, 341, 585, 654, 701 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 215, 247 |
| FatI | CATG | 3 cut(s) | 583, 652, 699 |
| Fnu4HI | GCNGC | 3 cut(s) | 77, 150, 501 |
| FokI | GGATG | 3 cut(s) | 149, 239, 404 |
| FriOI | GRGCYC | 1 cut(s) | 255 |
| Fsp4HI | GCNGC | 3 cut(s) | 77, 150, 501 |
| GluI | GCNGC | 3 cut(s) | 77, 150, 501 |
| HaeIII | GGCC | 2 cut(s) | 554, 644 |
| Hin1II | CATG | 3 cut(s) | 587, 656, 703 |
| HinfI | GANTC | 1 cut(s) | 421 |
| HphI | GGTGA | 8 cut(s) | 50, 154, 259, 451, 458, 505, 511, 520 |
| Hpy166II | GTNNAC | 2 cut(s) | 69, 528 |
| Hpy188I | TCNGA | 3 cut(s) | 292, 369, 382 |
| Hpy188III | TCNNGA | 3 cut(s) | 56, 97, 242 |
| Hpy8I | GTNNAC | 2 cut(s) | 69, 528 |
| HpyAV | CCTTC | 4 cut(s) | 139, 456, 526, 557 |
| HpyCH4III | ACNGT | 1 cut(s) | 706 |
| HpyCH4V | TGCA | 2 cut(s) | 299, 652 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 155 |
| HpyF3I | CTNAG | 4 cut(s) | 72, 368, 549, 633 |
| Hsp92II | CATG | 3 cut(s) | 587, 656, 703 |
| Kzo9I | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| LmnI | GCTCC | 1 cut(s) | 674 |
| LpnPI | CCDG | 7 cut(s) | 69, 118, 122, 145, 373, 400, 689 |
| Lsp1109I | GCAGC | 2 cut(s) | 88, 136 |
| LweI | GCATC | 2 cut(s) | 205, 337 |
| MaeIII | GTNAC | 1 cut(s) | 265 |
| MalI | GATC | 5 cut(s) | 83, 366, 384, 444, 663 |
| MbiI | CCGCTC | 1 cut(s) | 464 |
| MboI | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| MboII | GAAGA | 2 cut(s) | 337, 533 |
| MflI | RGATCY | 2 cut(s) | 442, 661 |
| MhlI | GDGCHC | 1 cut(s) | 255 |
| MluCI | AATT | 2 cut(s) | 404, 495 |
| MseI | TTAA | 1 cut(s) | 354 |
| MspA1I | CMGCKG | 1 cut(s) | 362 |
| MspR9I | CCNGG | 2 cut(s) | 133, 388 |
| Mva1269I | GAATGC | 1 cut(s) | 435 |
| MvaI | CCWGG | 2 cut(s) | 133, 388 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 155 |
| NdeII | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| NlaIII | CATG | 3 cut(s) | 587, 656, 703 |
| NlaIV | GGNNCC | 1 cut(s) | 374 |
| NmuCI | GTSAC | 1 cut(s) | 265 |
| NspI | RCATGY | 1 cut(s) | 587 |
| PasI | CCCWGGG | 1 cut(s) | 387 |
| PciI | ACATGT | 1 cut(s) | 583 |
| PctI | GAATGC | 1 cut(s) | 435 |
| PfeI | GAWTC | 1 cut(s) | 421 |
| PflFI | GACNNNGTC | 1 cut(s) | 331 |
| PkrI | GCNGC | 3 cut(s) | 78, 151, 502 |
| PpuMI | RGGWCCY | 1 cut(s) | 53 |
| PscI | ACATGT | 1 cut(s) | 583 |
| Psp124BI | GAGCTC | 1 cut(s) | 255 |
| Psp5II | RGGWCCY | 1 cut(s) | 53 |
| Psp6I | CCWGG | 2 cut(s) | 131, 386 |
| PspGI | CCWGG | 2 cut(s) | 131, 386 |
| PspN4I | GGNNCC | 1 cut(s) | 374 |
| PspPI | GGNCC | 2 cut(s) | 53, 643 |
| PspPPI | RGGWCCY | 1 cut(s) | 53 |
| PsuI | RGATCY | 2 cut(s) | 442, 661 |
| PsyI | GACNNNGTC | 1 cut(s) | 331 |
| PvuII | CAGCTG | 1 cut(s) | 362 |
| RsaI | GTAC | 1 cut(s) | 68 |
| RsaNI | GTAC | 1 cut(s) | 67 |
| SacI | GAGCTC | 1 cut(s) | 255 |
| SaqAI | TTAA | 1 cut(s) | 354 |
| SatI | GCNGC | 3 cut(s) | 77, 150, 501 |
| Sau3AI | GATC | 5 cut(s) | 81, 364, 382, 442, 661 |
| Sau96I | GGNCC | 2 cut(s) | 53, 643 |
| ScrFI | CCNGG | 2 cut(s) | 133, 388 |
| SduI | GDGCHC | 1 cut(s) | 255 |
| SfaNI | GCATC | 2 cut(s) | 205, 337 |
| SfcI | CTRYAG | 1 cut(s) | 184 |
| SinI | GGWCC | 1 cut(s) | 53 |
| SmlI | CTYRAG | 1 cut(s) | 242 |
| SmoI | CTYRAG | 1 cut(s) | 242 |
| Sse9I | AATT | 2 cut(s) | 404, 495 |
| SsiI | CCGC | 3 cut(s) | 462, 501, 687 |
| SspI | AATATT | 1 cut(s) | 91 |
| SstI | GAGCTC | 1 cut(s) | 255 |
| StyD4I | CCNGG | 2 cut(s) | 131, 386 |
| StyI | CCWWGG | 2 cut(s) | 555, 612 |
| TaaI | ACNGT | 1 cut(s) | 706 |
| TaqI | TCGA | 3 cut(s) | 255, 424, 602 |
| TaqII | GACCGA | 1 cut(s) | 263 |
| TasI | AATT | 2 cut(s) | 404, 495 |
| TauI | GCSGC | 1 cut(s) | 503 |
| TfiI | GAWTC | 1 cut(s) | 421 |
| Tru1I | TTAA | 1 cut(s) | 354 |
| Tru9I | TTAA | 1 cut(s) | 354 |
| TscAI | CASTG | 1 cut(s) | 76 |
| TseFI | GTSAC | 1 cut(s) | 265 |
| TseI | GCWGC | 2 cut(s) | 76, 149 |
| Tsp45I | GTSAC | 1 cut(s) | 265 |
| TspDTI | ATGAA | 1 cut(s) | 273 |
| TspGWI | ACGGA | 1 cut(s) | 129 |
| TspRI | CASTG | 1 cut(s) | 76 |
| Tth111I | GACNNNGTC | 1 cut(s) | 331 |
| VpaK11BI | GGWCC | 1 cut(s) | 53 |
| XceI | RCATGY | 1 cut(s) | 587 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 384 |