RchiOBHm_Chr6g0278331

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000732
Physical Location & Seq
Forward (+)
41393948 .. 41394322
375 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24966

Sequence Viewer

Length: 282 bp
ATGGTGCGTCAGGGCACTGTGTTTAAGGATGTTCCGTACACTGAGCAGCAGATCAGAAATATTTTCAAAGGTTTTGACCTGAATGGAGACGGCAAACTGTCCTGGGAAGAGGTGAAGGCTGCGTTCGCTAAACTCGGGGCGTTTTTGCCCGACTACAGAGCTTGGCGAGGACGAAGTTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCACAAACTACAGTATAAACCGAATCGAAGTAATTAA

Protein Analysis

93

Amino Acids

10.71

Weight (kDa)

7.83

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 10 - 42 4.1e-06 EF-hand domain pair
EF-hand_8 PF13833 14 - 39 3.1e-06 EF-hand domain pair
EF-hand_6 PF13405 17 - 46 3e-07 EF-hand domain
EF-hand_1 PF00036 18 - 45 2.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 38
AgsI TTSAA 1 cut(s) 67
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 2 cut(s) 161, 223
AluI AGCT 2 cut(s) 161, 223
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 81, 209
Ama87I CYCGRG 2 cut(s) 134, 212
ApeKI GCWGC 2 cut(s) 46, 119
AsuHPI GGTGA 2 cut(s) 124, 229
AvaI CYCGRG 2 cut(s) 134, 212
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 58, 106
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 81, 209
BfmI CTRYAG 2 cut(s) 154, 254
BisI GCNGC 2 cut(s) 47, 120
BlsI GCNGC 2 cut(s) 48, 121
Bme1390I CCNGG 1 cut(s) 103
BmeT110I CYCGRG 2 cut(s) 134, 212
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 34, 202
BseMII CTCAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 2 cut(s) 58, 106
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 2 cut(s) 134, 212
BsmAI GTCTC 2 cut(s) 81, 209
BsmBI CGTCTC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 2 cut(s) 134, 212
Bsp1286I GDGCHC 2 cut(s) 17, 225
Bsp143I GATC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 3 cut(s) 19, 99, 258
Bst6I CTCTTC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 34, 202
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 2 cut(s) 81, 209
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 2 cut(s) 154, 254
BstSLI GKGCMC 1 cut(s) 17
BstV1I GCAGC 2 cut(s) 58, 106
BtsCI GGATG 2 cut(s) 34, 202
BtsIMutI CAGTG 2 cut(s) 15, 39
Csp6I GTAC 1 cut(s) 37
CviJI RGCY 4 cut(s) 119, 161, 201, 223
CviKI_1 RGCY 4 cut(s) 119, 161, 201, 223
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 42
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 2 cut(s) 134, 212
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 81
FaiI YATR 2 cut(s) 243, 261
FalI AAGNNNNNCTT 2 cut(s) 185, 217
Fnu4HI GCNGC 2 cut(s) 47, 120
FokI GGATG 2 cut(s) 41, 209
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 2 cut(s) 47, 120
GluI GCNGC 2 cut(s) 47, 120
HinfI GANTC 1 cut(s) 268
HphI GGTGA 2 cut(s) 124, 229
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 1 cut(s) 56
Hpy188III TCNNGA 2 cut(s) 212, 214
Hpy8I GTNNAC 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 3 cut(s) 19, 99, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 1 cut(s) 42
Kzo9I GATC 1 cut(s) 51
LpnPI CCDG 3 cut(s) 88, 92, 115
Lsp1109I GCAGC 2 cut(s) 58, 106
LweI GCATC 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 119
MhlI GDGCHC 2 cut(s) 17, 225
MluCI AATT 1 cut(s) 277
MnlI CCTC 2 cut(s) 103, 161
MseI TTAA 2 cut(s) 24, 280
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeII GATC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 212
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 2 cut(s) 48, 121
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 2 cut(s) 24, 280
SatI GCNGC 2 cut(s) 47, 120
Sau3AI GATC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 2 cut(s) 17, 225
SetI ASST 6 cut(s) 73, 81, 114, 163, 225, 242
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 2 cut(s) 154, 254
Sfr274I CTCGAG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 1 cut(s) 277
SspI AATATT 1 cut(s) 61
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 101
TaaI ACNGT 3 cut(s) 19, 99, 258
TaqI TCGA 2 cut(s) 213, 271
TaqII GACCGA 1 cut(s) 233
TasI AATT 1 cut(s) 277
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 2 cut(s) 24, 280
Tru9I TTAA 2 cut(s) 24, 280
TscAI CASTG 2 cut(s) 22, 46
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 2 cut(s) 46, 119
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 193, 243
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 22, 46
XhoI CTCGAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.