Rh6BG480800

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000732
Physical Location & Seq
Reverse (-)
68661640 .. 68661984
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG480800.1

Sequence Viewer

Length: 345 bp
ATGGTTAGGGATGTCGGTCTTCCCTCTTTTAAACCAGAATTTGAAAGATCTGCTTCTTGTTTTGCACCAACCTGCTGGCCTGAGCAGACGGTCATTAAGGAGGTCCCAGTTCTCTGGAGCAAGGAGCAAATCTACGATGTTTTCAAAAGCTTTGATAGGAACGGTGACGGCAAACTGTCCAAGGATGAGCTCAAGGCAGCCTTTAGAAATCTCGGATCGCGTTGGAGCTCTTACAGAGCTAGGAGAGCATTACGCCATGTAGATGCCAACGGCGACGGCATCATATCTAATGAAGAGCTCAACGACCTTATCAAATATGCCCTAGAATGTGGTTATAAGCTATAA

Protein Analysis

114

Amino Acids

13.08

Weight (kDa)

7.68

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 41 - 104 7.8e-11 EF-hand domain pair
EF-hand_6 PF13405 44 - 72 2.8e-09 EF-hand domain
EF-hand_1 PF00036 44 - 70 1.9e-07 EF hand domain
EF-hand_5 PF13202 44 - 66 5.8e-08 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
Acc36I ACCTGC 1 cut(s) 80
AccII CGCG 1 cut(s) 220
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 1 cut(s) 38
AgsI TTSAA 2 cut(s) 44, 145
AluBI AGCT 6 cut(s) 150, 190, 228, 239, 298, 340
AluI AGCT 6 cut(s) 150, 190, 228, 239, 298, 340
Alw21I GWGCWC 3 cut(s) 192, 230, 300
AlwI GGATC 1 cut(s) 223
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 1 cut(s) 176
AvaII GGWCC 1 cut(s) 103
BanII GRGCYC 3 cut(s) 192, 230, 300
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 3 cut(s) 192, 230, 300
BbvI GCAGC 1 cut(s) 209
BceAI ACGGC 3 cut(s) 184, 286, 292
BfaI CTAG 2 cut(s) 240, 323
BfuAI ACCTGC 1 cut(s) 80
BglII AGATCT 1 cut(s) 47
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 105
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 2 cut(s) 253, 288
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 180
Bse1I ACTGG 1 cut(s) 107
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 16, 190
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 1 cut(s) 79
BsiHKAI GWGCWC 3 cut(s) 192, 230, 300
BslFI GGGAC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 79
Bsp1286I GDGCHC 3 cut(s) 192, 230, 300
Bsp143I GATC 2 cut(s) 47, 215
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 105
BspMI ACCTGC 1 cut(s) 80
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 288
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 2 cut(s) 47, 215
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 3 cut(s) 91, 164, 177
Bst6I CTCTTC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 16, 190
BstFNI CGCG 1 cut(s) 220
BstKTI GATC 2 cut(s) 50, 218
BstMBI GATC 2 cut(s) 47, 215
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 47
BstXI CCANNNNNNTGG 2 cut(s) 75, 114
BstYI RGATCY 1 cut(s) 47
BsuRI GGCC 1 cut(s) 79
BtsCI GGATG 2 cut(s) 16, 190
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 103
CviAII CATG 1 cut(s) 257
CviJI RGCY 8 cut(s) 79, 150, 190, 200, 228, 239, 298, 340
CviKI_1 RGCY 8 cut(s) 79, 150, 190, 200, 228, 239, 298, 340
DdeI CTNAG 1 cut(s) 81
DpnI GATC 2 cut(s) 49, 217
DpnII GATC 2 cut(s) 47, 215
DraI TTTAAA 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Ecl136II GAGCTC 3 cut(s) 190, 228, 298
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 3 cut(s) 192, 230, 300
Eco47I GGWCC 1 cut(s) 103
Eco53kI GAGCTC 3 cut(s) 190, 228, 298
EcoICRI GAGCTC 3 cut(s) 190, 228, 298
EcoO109I RGGNCCY 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 3 cut(s) 192, 230, 300
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 1 cut(s) 260
FaiI YATR 5 cut(s) 258, 284, 318, 336, 343
FalI AAGNNNNNCTT 4 cut(s) 37, 69, 185, 217
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 2 cut(s) 23, 197
FriOI GRGCYC 3 cut(s) 192, 230, 300
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 240, 323
GluI GCNGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 136
HaeIII GGCC 1 cut(s) 79
Hin1II CATG 1 cut(s) 260
HindIII AAGCTT 1 cut(s) 148
HphI GGTGA 1 cut(s) 176
Hpy188I TCNGA 1 cut(s) 215
Hpy188III TCNNGA 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 3 cut(s) 91, 164, 177
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 81
Hsp92II CATG 1 cut(s) 260
Kzo9I GATC 2 cut(s) 47, 215
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 3 cut(s) 117, 124, 225
LpnPI CCDG 6 cut(s) 48, 61, 85, 93, 100, 120
Lsp1109I GCAGC 1 cut(s) 209
LweI GCATC 2 cut(s) 253, 288
MaeI CTAG 2 cut(s) 240, 323
MaeIII GTNAC 1 cut(s) 164
MalI GATC 2 cut(s) 49, 217
MboI GATC 2 cut(s) 47, 215
MboII GAAGA 2 cut(s) 11, 305
MflI RGATCY 1 cut(s) 47
MhlI GDGCHC 3 cut(s) 192, 230, 300
MluCI AATT 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 2 cut(s) 34, 94
MseI TTAA 2 cut(s) 30, 96
MslI CAYNNNNRTG 1 cut(s) 261
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 245
NdeII GATC 2 cut(s) 47, 215
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 288
PkrI GCNGC 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 103
PsiI TTATAA 1 cut(s) 336
Psp124BI GAGCTC 3 cut(s) 192, 230, 300
Psp5II RGGWCCY 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 1 cut(s) 103
PspPPI RGGWCCY 1 cut(s) 103
PsuI RGATCY 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 261
SacI GAGCTC 3 cut(s) 192, 230, 300
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 30, 96
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 2 cut(s) 47, 215
Sau96I GGNCC 1 cut(s) 103
SduI GDGCHC 3 cut(s) 192, 230, 300
SetI ASST 9 cut(s) 74, 105, 152, 192, 230, 241, 300, 309, 342
SfaNI GCATC 2 cut(s) 253, 288
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 38
SspMI CTAG 2 cut(s) 240, 323
SstI GAGCTC 3 cut(s) 192, 230, 300
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 91, 164, 177
TaqII GACCGA 1 cut(s) 5
TasI AATT 1 cut(s) 38
Tru1I TTAA 2 cut(s) 30, 96
Tru9I TTAA 2 cut(s) 30, 96
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 1 cut(s) 38
XspI CTAG 2 cut(s) 240, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.