Rroxscaffold_7G00160830

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000732
Physical Location & Seq
Forward (+)
3721724 .. 3723688
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160830.1

Sequence Viewer

Length: 321 bp
ATGGCCAAGATGGAGTCGGGTTTGAGAGTGGACTCGAGGAAGCAGAAGGCCATTAAGAAGGCCCCAATTCACTGTATCAGCAAGGAGCAAGTCTGCGATTTTTTCAAAAGATTTGATAGGAACGGTGACGGCAAACTCTGCAAGGAAGAGATCAAGGCAGCCTTTCGGAAACTCGGATCACGTTGGAGCTCTTACAGAGCCCGGAGAGCATTAGACCATGCAGATTCCAATGGCGATGGCATCATCTCTAATGAGGAGCTCGACGACCTTATCAACTATGCCCTAGATTTTGGTTATAAGCTATTAGCGCCACATATATAG

Protein Analysis

106

Amino Acids

12.12

Weight (kDa)

9.22

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 33 - 59 2.3e-07 EF-hand domain
EF-hand_1 PF00036 33 - 58 2.4e-06 EF hand domain
EF-hand_5 PF13202 33 - 53 2.7e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AclWI GGATC 1 cut(s) 184
AcoI YGGCCR 1 cut(s) 3
AgsI TTSAA 1 cut(s) 106
AluBI AGCT 3 cut(s) 189, 259, 301
AluI AGCT 3 cut(s) 189, 259, 301
Alw21I GWGCWC 2 cut(s) 191, 261
AlwI GGATC 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 3 cut(s) 3, 48, 60
ApeKI GCWGC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 34
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 191, 202, 261
Bbv12I GWGCWC 2 cut(s) 191, 261
BbvI GCAGC 1 cut(s) 170
BccI CCATC 2 cut(s) 4, 230
BceAI ACGGC 1 cut(s) 145
BcnI CCSGG 1 cut(s) 202
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 311
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 170
BshFI GGCC 3 cut(s) 5, 50, 62
BsiHKAI GWGCWC 2 cut(s) 191, 261
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 1 cut(s) 202
BsnI GGCC 3 cut(s) 5, 50, 62
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 191, 202, 261
Bsp143I GATC 2 cut(s) 150, 176
BspANI GGCC 3 cut(s) 5, 50, 62
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 1 cut(s) 184
BssMI GATC 2 cut(s) 150, 176
Bst4CI ACNGT 2 cut(s) 74, 125
Bst6I CTCTTC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 2 cut(s) 153, 179
BstMBI GATC 2 cut(s) 150, 176
BstMWI GCNNNNNNNGC 3 cut(s) 138, 206, 307
BstSCI CCNGG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 170
BsuRI GGCC 3 cut(s) 5, 50, 62
BtgZI GCGATG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 70
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 61
CviAII CATG 1 cut(s) 218
CviJI RGCY 8 cut(s) 5, 50, 62, 161, 189, 200, 259, 301
CviKI_1 RGCY 8 cut(s) 5, 50, 62, 161, 189, 200, 259, 301
DpnI GATC 2 cut(s) 152, 178
DpnII GATC 2 cut(s) 150, 176
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Ecl136II GAGCTC 2 cut(s) 189, 259
Eco24I GRGCYC 3 cut(s) 191, 202, 261
Eco53kI GAGCTC 2 cut(s) 189, 259
Eco88I CYCGRG 1 cut(s) 34
EcoICRI GAGCTC 2 cut(s) 189, 259
EcoO109I RGGNCCY 1 cut(s) 61
EcoT38I GRGCYC 3 cut(s) 191, 202, 261
FaeI CATG 1 cut(s) 221
FaiI YATR 6 cut(s) 219, 279, 297, 315, 317, 319
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FatI CATG 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 159
FriOI GRGCYC 3 cut(s) 191, 202, 261
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 309
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 3 cut(s) 5, 50, 62
HapII CCGG 1 cut(s) 202
HhaI GCGC 1 cut(s) 310
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HinfI GANTC 3 cut(s) 14, 32, 224
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 168, 176
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 40, 52
HpyCH4III ACNGT 2 cut(s) 74, 125
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 141, 221
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 206, 307
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 1 cut(s) 308
Kzo9I GATC 2 cut(s) 150, 176
LmnI GCTCC 3 cut(s) 85, 186, 256
LpnPI CCDG 1 cut(s) 215
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 284
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 125
MalI GATC 2 cut(s) 152, 178
MboI GATC 2 cut(s) 150, 176
MboII GAAGA 1 cut(s) 158
MhlI GDGCHC 3 cut(s) 191, 202, 261
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 23, 26
MmeI TCCRAC 1 cut(s) 164
MnlI CCTC 2 cut(s) 30, 247
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 138, 206, 307
NciI CCSGG 1 cut(s) 202
NdeII GATC 2 cut(s) 150, 176
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 125
PaeR7I CTCGAG 1 cut(s) 34
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 2 cut(s) 22, 26
PpsI GAGTC 2 cut(s) 22, 26
PsiI TTATAA 1 cut(s) 297
Psp124BI GAGCTC 2 cut(s) 191, 261
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 34
SacI GAGCTC 2 cut(s) 191, 261
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 150, 176
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 2 cut(s) 23, 26
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 3 cut(s) 191, 202, 261
SetI ASST 5 cut(s) 184, 191, 261, 270, 303
SfaNI GCATC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 2 cut(s) 191, 261
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 2 cut(s) 74, 125
TaiI ACGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 35, 261
TasI AATT 1 cut(s) 66
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 125
TspRI CASTG 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 34
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.