RLG00000013236

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000732
Physical Location & Seq
Reverse (-)
28139237 .. 28140948
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013236

Sequence Viewer

Length: 393 bp
ATGGTGCGTGAGGTCACTGTGGTGTCTAAGGATCTTCCGTACACTGAGCAGCAGATCATAAATATTTTCAAAGGTTTTGACCTAAATGGAGACGGCAAACTATCCTGGGATGAGGTGAAGGCTGCGTTCGCTAAACTTGGGGCGTTTTCACCCGACTACAGAGCTTGGCGAGGACGAAGCTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCACAAACTACAGTATAAACCGAATCATTCGTTTTGTGTTCTGGTTCTTGCAAAGCTTAGTCGAGGATCGCCGTACAAGGTCAATTATGGGTATCAAATTGCCAAGGATATCTTTTTGGAAGCCAAACACTATCGTGGTGGTTAA

Protein Analysis

131

Amino Acids

14.77

Weight (kDa)

8.48

Isoelectric Point (pI)

23.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 19 - 46 2.4e-06 EF hand domain
EF-hand_6 PF13405 19 - 47 2.2e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 39, 322
AfaI GTAC 2 cut(s) 41, 323
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 104
AleI CACNNNNGTG 2 cut(s) 20, 381
AluBI AGCT 4 cut(s) 164, 180, 226, 304
AluI AGCT 4 cut(s) 164, 180, 226, 304
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 84, 212
AlwI GGATC 2 cut(s) 39, 322
Ama87I CYCGRG 1 cut(s) 215
ApeKI GCWGC 2 cut(s) 49, 122
AsuHPI GGTGA 3 cut(s) 127, 141, 232
AvaI CYCGRG 1 cut(s) 215
BanII GRGCYC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 61, 109
BccI CCATC 1 cut(s) 194
BceAI ACGGC 2 cut(s) 109, 304
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 84, 212
BfmI CTRYAG 2 cut(s) 157, 257
BisI GCNGC 2 cut(s) 50, 123
BlsI GCNGC 2 cut(s) 51, 124
Bme1390I CCNGG 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 178
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 105, 351
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 105, 351
BseGI GGATG 2 cut(s) 115, 205
BseMII CTCAG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 61, 109
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 84, 212
BsmBI CGTCTC 1 cut(s) 84
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 31, 54, 314
BspCNI CTCAG 1 cut(s) 37
BspPI GGATC 2 cut(s) 39, 322
BspTNI GGTCTC 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 105, 351
BssMI GATC 3 cut(s) 31, 54, 314
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 19, 261
BstDEI CTNAG 3 cut(s) 27, 45, 305
BstF5I GGATG 2 cut(s) 115, 205
BstKTI GATC 3 cut(s) 34, 57, 317
BstMAI GTCTC 2 cut(s) 84, 212
BstMBI GATC 3 cut(s) 31, 54, 314
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 2 cut(s) 157, 257
BstV1I GCAGC 2 cut(s) 61, 109
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BtsCI GGATG 2 cut(s) 115, 205
BtsIMutI CAGTG 2 cut(s) 15, 42
Csp6I GTAC 2 cut(s) 40, 322
CviJI RGCY 7 cut(s) 122, 164, 180, 204, 226, 304, 371
CviKI_1 RGCY 7 cut(s) 122, 164, 180, 204, 226, 304, 371
CviQI GTAC 2 cut(s) 40, 322
DdeI CTNAG 3 cut(s) 27, 45, 305
DpnI GATC 3 cut(s) 33, 56, 316
DpnII GATC 3 cut(s) 31, 54, 314
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 212
Eco32I GATATC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 104
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 84
FaiI YATR 4 cut(s) 59, 246, 264, 336
FalI AAGNNNNNCTT 4 cut(s) 188, 220, 344, 376
Fnu4HI GCNGC 2 cut(s) 50, 123
FokI GGATG 2 cut(s) 122, 212
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 2 cut(s) 50, 123
GluI GCNGC 2 cut(s) 50, 123
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 1 cut(s) 271
HphI GGTGA 3 cut(s) 127, 141, 232
Hpy166II GTNNAC 1 cut(s) 42
Hpy188III TCNNGA 2 cut(s) 215, 217
Hpy8I GTNNAC 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 19, 261
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 3 cut(s) 27, 45, 305
Kzo9I GATC 3 cut(s) 31, 54, 314
LpnPI CCDG 3 cut(s) 91, 118, 275
Lsp1109I GCAGC 2 cut(s) 61, 109
LweI GCATC 1 cut(s) 178
MaeIII GTNAC 2 cut(s) 13, 238
MalI GATC 3 cut(s) 33, 56, 316
MboI GATC 3 cut(s) 31, 54, 314
MboII GAAGA 1 cut(s) 26
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 331, 345
MnlI CCTC 4 cut(s) 4, 106, 164, 305
MseI TTAA 1 cut(s) 391
MslI CAYNNNNRTG 2 cut(s) 20, 381
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeII GATC 3 cut(s) 31, 54, 314
NmuCI GTSAC 2 cut(s) 13, 238
OliI CACNNNNGTG 2 cut(s) 20, 381
PaeR7I CTCGAG 1 cut(s) 215
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 2 cut(s) 51, 124
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 2 cut(s) 41, 323
RsaNI GTAC 2 cut(s) 40, 322
RseI CAYNNNNRTG 2 cut(s) 20, 381
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 391
SatI GCNGC 2 cut(s) 50, 123
Sau3AI GATC 3 cut(s) 31, 54, 314
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 2 cut(s) 157, 257
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 20, 381
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 331, 345
SspI AATATT 1 cut(s) 64
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 19, 261
TaqI TCGA 2 cut(s) 216, 310
TaqII GACCGA 1 cut(s) 236
TasI AATT 2 cut(s) 331, 345
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 391
Tru9I TTAA 1 cut(s) 391
TscAI CASTG 2 cut(s) 22, 49
TseFI GTSAC 2 cut(s) 13, 238
TseI GCWGC 2 cut(s) 49, 122
Tsp45I GTSAC 2 cut(s) 13, 238
TspDTI ATGAA 2 cut(s) 196, 246
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 2 cut(s) 22, 49
XhoI CTCGAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.