Rh4AG313500

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000732
Physical Location & Seq
Reverse (-)
62375365 .. 62375798
434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG313500.1

Sequence Viewer

Length: 318 bp
ATGCCTCAGCGGAAAGATTGTAAGATGAGGCACCCAGCTATTGTGCATAGATGTCCTGATGTTCCGTACACTGAGAAGCAGATCATAAATATTTTCAAGAGCTTTGACCTGAATGGAGACGGCAAGCTGTCCTGGGATGAGGTGAAGGCTGCGTTTGCTAAACTTGGGGCAAACTTGCCCTACTACAGAGCTTGGCGAGGACGAATCTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCTAAAACAAAGACGCATGAGCGTCCTGGTGTGTAAGCAGTAA

Protein Analysis

105

Amino Acids

12.16

Weight (kDa)

9.14

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 18 - 52 3.5e-06 EF-hand domain pair
EF-hand_1 PF00036 28 - 55 4.8e-07 EF hand domain
EF-hand_6 PF13405 28 - 56 4.1e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 30
AciI CCGC 1 cut(s) 10
AfaI GTAC 1 cut(s) 68
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 131, 300
AluBI AGCT 5 cut(s) 38, 102, 127, 191, 253
AluI AGCT 5 cut(s) 38, 102, 127, 191, 253
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 111, 239
Ama87I CYCGRG 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 154, 259
AvaI CYCGRG 1 cut(s) 242
BanI GGYRCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 1 cut(s) 136
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 133, 302
BcoDI GTCTC 2 cut(s) 111, 239
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 133, 302
BmeT110I CYCGRG 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 2 cut(s) 133, 302
BmsI GCATC 1 cut(s) 205
Bpu10I CCTNAGC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 132
BseBI CCWGG 2 cut(s) 133, 302
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 142, 232
BseMII CTCAG 2 cut(s) 20, 63
BseXI GCAGC 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 34
BshNI GGYRCC 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 111, 239
BsmBI CGTCTC 1 cut(s) 111
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 1 cut(s) 10
BspCNI CTCAG 2 cut(s) 19, 64
BspLI GGNNCC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 133, 302
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 6, 72
BstF5I GGATG 2 cut(s) 142, 232
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 111, 239
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 2 cut(s) 133, 302
BstSCI CCNGG 2 cut(s) 131, 300
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 136
BtsCI GGATG 2 cut(s) 142, 232
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 125
CseI GACGC 2 cut(s) 286, 297
Csp6I GTAC 1 cut(s) 67
CviAII CATG 1 cut(s) 292
CviJI RGCY 7 cut(s) 38, 102, 127, 149, 191, 231, 253
CviKI_1 RGCY 7 cut(s) 38, 102, 127, 149, 191, 231, 253
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 2 cut(s) 6, 72
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 131, 300
EcoT38I GRGCYC 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 1 cut(s) 295
FaiI YATR 4 cut(s) 48, 86, 273, 293
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 2 cut(s) 149, 239
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
GsaI CCCAGC 1 cut(s) 38
HgaI GACGC 2 cut(s) 286, 297
Hin1II CATG 1 cut(s) 295
HinfI GANTC 1 cut(s) 204
HphI GGTGA 2 cut(s) 154, 259
Hpy166II GTNNAC 1 cut(s) 69
Hpy188III TCNNGA 4 cut(s) 56, 97, 242, 244
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 6, 72
Hsp92II CATG 1 cut(s) 295
Kzo9I GATC 1 cut(s) 81
LpnPI CCDG 7 cut(s) 48, 69, 118, 122, 145, 287, 314
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 255
MnlI CCTC 4 cut(s) 15, 21, 133, 191
MspA1I CMGCKG 1 cut(s) 10
MspR9I CCNGG 2 cut(s) 133, 302
MvaI CCWGG 2 cut(s) 133, 302
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 1 cut(s) 81
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 242
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 131, 300
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 131, 300
PspN4I GGNNCC 1 cut(s) 32
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SacI GAGCTC 1 cut(s) 255
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 133, 302
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 40, 104, 111, 129, 144, 193, 255, 272
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SsiI CCGC 1 cut(s) 10
SspI AATATT 1 cut(s) 91
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 131, 300
TaqI TCGA 1 cut(s) 243
TaqII GACCGA 1 cut(s) 263
TfiI GAWTC 1 cut(s) 204
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 223, 273
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 76
XhoI CTCGAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.