pycom08g08360

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000732
Physical Location & Seq
Forward (+)
6714608 .. 6715116
509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08360.2

Sequence Viewer

Length: 423 bp
ATGCCGGCCAGGCCGCTGGGTACACCCCTATATAAATATAGTGCAAGTCACTCATCAATTCATGCCAAGCCTTTTAGTTTTCTTGTAGTGTGTAGAACAAAACACAGAAGAAAATTTCAAGATCACCATATATATCTATATACTATCATGGTTAGGGATGTCATTCATAAGGAGCCAACCGGTGTTCCATGGACTAGGGAGCAGGTGGTGAATCTCTTCAATAGTTTTGACTCGGACGGCGACGGCATGTTGTCCCAAGAGGAGCTGAAGGCAGCCTTCAGAAAGCTCGGGTCGCGTTGGGGTGGTTTCAGGGCGAGGAGAGCTTTGGGTCACGCAGATGCCAACCATGACGGGATCATATCTAAAGAGGAGATCAAAAACCTTGTCAACTATGCCCTAGACTGTGGGTATAAGCTAAATTAA

Protein Analysis

141

Amino Acids

16.04

Weight (kDa)

9.58

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 193
AccII CGCG 1 cut(s) 295
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 2 cut(s) 262, 287
AfaI GTAC 1 cut(s) 22
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 119, 220
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 4 cut(s) 265, 286, 323, 415
AluI AGCT 4 cut(s) 265, 286, 323, 415
AlwI GGATC 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 6, 11
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 1 cut(s) 113
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AsiGI ACCGGT 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 116, 220
AvaI CYCGRG 1 cut(s) 287
BbvI GCAGC 1 cut(s) 284
BceAI ACGGC 2 cut(s) 253, 259
BciT130I CCWGG 1 cut(s) 10
BfaI CTAG 2 cut(s) 195, 398
BfuAI ACCTGC 1 cut(s) 193
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 2 cut(s) 14, 273
BlsI GCNGC 2 cut(s) 15, 274
Bme1390I CCNGG 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 179
Bse118I RCCGGY 2 cut(s) 4, 179
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 1 cut(s) 163
BseRI GAGGAG 3 cut(s) 275, 331, 383
BseXI GCAGC 1 cut(s) 284
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 2 cut(s) 8, 13
BshTI ACCGGT 1 cut(s) 179
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 5, 180
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 8, 13
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 3 cut(s) 121, 354, 372
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 8, 13
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 174
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 362
BsrFI RCCGGY 2 cut(s) 4, 179
BssAI RCCGGY 2 cut(s) 4, 179
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 121, 354, 372
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 295
BstKTI GATC 3 cut(s) 124, 357, 375
BstMBI GATC 3 cut(s) 121, 354, 372
BstMWI GCNNNNNNNGC 3 cut(s) 10, 292, 320
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 8
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 16
BsuRI GGCC 2 cut(s) 8, 13
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 163
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 6
Cfr10I RCCGGY 2 cut(s) 4, 179
Csp6I GTAC 1 cut(s) 21
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 5 cut(s) 62, 148, 189, 247, 347
CviJI RGCY 9 cut(s) 8, 13, 70, 175, 265, 275, 286, 323, 415
CviKI_1 RGCY 9 cut(s) 8, 13, 70, 175, 265, 275, 286, 323, 415
CviQI GTAC 1 cut(s) 21
DpnI GATC 3 cut(s) 123, 356, 374
DpnII GATC 3 cut(s) 121, 354, 372
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 262, 287
Eco88I CYCGRG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 5 cut(s) 65, 151, 192, 250, 350
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 61, 147, 188, 246, 346
Fnu4HI GCNGC 2 cut(s) 14, 273
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 14, 273
FspBI CTAG 2 cut(s) 195, 398
GluI GCNGC 2 cut(s) 14, 273
GsaI CCCAGC 1 cut(s) 20
HaeIII GGCC 2 cut(s) 8, 13
HapII CCGG 2 cut(s) 5, 180
Hin1II CATG 5 cut(s) 65, 151, 192, 250, 350
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 2 cut(s) 211, 230
HpaII CCGG 2 cut(s) 5, 180
HphI GGTGA 2 cut(s) 116, 220
Hpy166II GTNNAC 2 cut(s) 23, 388
Hpy188I TCNGA 2 cut(s) 235, 281
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 2 cut(s) 23, 388
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 262, 286
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 292, 320
Hsp92II CATG 5 cut(s) 65, 151, 192, 250, 350
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 121, 354, 372
LmnI GCTCC 3 cut(s) 172, 199, 262
LpnPI CCDG 6 cut(s) 2, 18, 22, 188, 193, 295
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 195, 398
MaeIII GTNAC 2 cut(s) 47, 329
MalI GATC 3 cut(s) 123, 356, 374
MboI GATC 3 cut(s) 121, 354, 372
MboII GAAGA 2 cut(s) 120, 208
MluCI AATT 3 cut(s) 57, 113, 418
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 3 cut(s) 253, 309, 361
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 1 cut(s) 421
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 5, 180
MspR9I CCNGG 1 cut(s) 10
MvaI CCWGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 3 cut(s) 10, 292, 320
NaeI GCCGGC 1 cut(s) 6
NcoI CCATGG 1 cut(s) 188
NdeII GATC 3 cut(s) 121, 354, 372
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 65, 151, 192, 250, 350
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 47, 329
NspI RCATGY 1 cut(s) 250
PaqCI CACCTGC 1 cut(s) 193
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 211
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 2 cut(s) 15, 274
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 174
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 2 cut(s) 14, 273
Sau3AI GATC 3 cut(s) 121, 354, 372
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 10
SetI ASST 6 cut(s) 207, 267, 288, 325, 384, 417
SfaNI GCATC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 336
Sse9I AATT 3 cut(s) 57, 113, 418
SsiI CCGC 1 cut(s) 14
SspMI CTAG 2 cut(s) 195, 398
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 404
TasI AATT 3 cut(s) 57, 113, 418
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TseFI GTSAC 2 cut(s) 47, 329
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 2 cut(s) 47, 329
TspDTI ATGAA 2 cut(s) 50, 155
XapI RAATTY 1 cut(s) 113
XceI RCATGY 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 2 cut(s) 195, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.