RLG00000010700

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000732
Physical Location & Seq
Forward (+)
3714682 .. 3715090
409 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010700

Sequence Viewer

Length: 324 bp
ATGGGGAGGGCAGACCGGCCGAAAAAAGACACGAGGCCTGAGTTTCCGCCGAAGGTTCCGTACACTAGGCAGCAGATCACAGATATTTTCAAAGGTTATGATAAGAACGGAGACGGCAAGCTCTCCTGGGATGAGGTGAAGGCTGCGTTCGCTAAACTCGGGGCGCGTTTGCCCGACTACAGAGCTTGGCGAGGACGAAGGTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCAAGACCGAGCTCAATGAACTTGTCACCTATACCCTGGAACTAGAGTATACACCGAAACGCATGAGCGTCCTGGTGTGTAAGCAGTAA

Protein Analysis

108

Amino Acids

12.37

Weight (kDa)

9.42

Isoelectric Point (pI)

22.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 26 - 53 7.5e-07 EF hand domain
EF-hand_6 PF13405 26 - 54 2.9e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 284
AccII CGCG 1 cut(s) 166
AciI CCGC 1 cut(s) 47
AcoI YGGCCR 1 cut(s) 17
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 3 cut(s) 125, 270, 306
AluBI AGCT 3 cut(s) 121, 185, 247
AluI AGCT 3 cut(s) 121, 185, 247
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 105
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 2 cut(s) 17, 35
ApeKI GCWGC 2 cut(s) 70, 143
AspLEI GCGC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 148, 253
AvaI CYCGRG 1 cut(s) 158
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BauI CACGAG 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 82, 130
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 3 cut(s) 127, 272, 308
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 2 cut(s) 66, 278
BfmI CTRYAG 1 cut(s) 178
BisI GCNGC 2 cut(s) 71, 144
BlsI GCNGC 2 cut(s) 72, 145
Bme1390I CCNGG 3 cut(s) 127, 272, 308
BmeT110I CYCGRG 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 3 cut(s) 127, 272, 308
BmsI GCATC 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 126, 270
Bse118I RCCGGY 1 cut(s) 15
BseBI CCWGG 3 cut(s) 127, 272, 308
BseDI CCNNGG 2 cut(s) 126, 270
BseGI GGATG 2 cut(s) 136, 226
BseMII CTCAG 1 cut(s) 30
BseX3I CGGCCG 1 cut(s) 17
BseXI GCAGC 2 cut(s) 82, 130
Bsh1236I CGCG 1 cut(s) 166
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 2 cut(s) 19, 37
BsiEI CGRYCG 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 105
BsmBI CGTCTC 1 cut(s) 105
BsnI GGCC 2 cut(s) 19, 37
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 1 cut(s) 75
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 19, 37
BspCNI CTCAG 1 cut(s) 31
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 126, 270
BssMI GATC 1 cut(s) 75
BssNAI GTATAC 1 cut(s) 285
BssSI CACGAG 1 cut(s) 31
Bst1107I GTATAC 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 3 cut(s) 127, 272, 308
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 136, 226
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 1 cut(s) 78
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 75
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 3 cut(s) 127, 272, 308
BstSCI CCNGG 3 cut(s) 125, 270, 306
BstSFI CTRYAG 1 cut(s) 178
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 82, 130
BstZ17I GTATAC 1 cut(s) 285
BstZI CGGCCG 1 cut(s) 17
BsuRI GGCC 2 cut(s) 19, 37
BtsCI GGATG 2 cut(s) 136, 226
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 15
CseI GACGC 1 cut(s) 292
Csp6I GTAC 1 cut(s) 61
CviAII CATG 1 cut(s) 298
CviJI RGCY 7 cut(s) 19, 37, 121, 143, 185, 225, 247
CviKI_1 RGCY 7 cut(s) 19, 37, 121, 143, 185, 225, 247
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 39
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
EaeI YGGCCR 1 cut(s) 17
EagI CGGCCG 1 cut(s) 17
EciI GGCGGA 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 17
Eco147I AGGCCT 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 249
Eco52I CGGCCG 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 3 cut(s) 125, 270, 306
EcoT38I GRGCYC 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 1 cut(s) 301
FaiI YATR 4 cut(s) 99, 267, 285, 299
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 1 cut(s) 297
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 2 cut(s) 71, 144
FokI GGATG 2 cut(s) 143, 233
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 71, 144
FspBI CTAG 2 cut(s) 66, 278
GlaI GCGC 1 cut(s) 165
GluI GCNGC 2 cut(s) 71, 144
HaeIII GGCC 2 cut(s) 19, 37
HapII CCGG 1 cut(s) 16
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 166
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HpaII CCGG 1 cut(s) 16
HphI GGTGA 2 cut(s) 148, 253
Hpy166II GTNNAC 2 cut(s) 63, 285
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 2 cut(s) 63, 285
HpyAV CCTTC 3 cut(s) 46, 133, 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 39
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 1 cut(s) 75
LpnPI CCDG 8 cut(s) 29, 51, 112, 139, 257, 284, 293, 320
Lsp1109I GCAGC 2 cut(s) 82, 130
LweI GCATC 1 cut(s) 199
MaeI CTAG 2 cut(s) 66, 278
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 249
MnlI CCTC 3 cut(s) 27, 127, 185
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 3 cut(s) 127, 272, 308
MvaI CCWGG 3 cut(s) 127, 272, 308
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 1 cut(s) 75
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 259
PceI AGGCCT 1 cut(s) 37
PkrI GCNGC 2 cut(s) 72, 145
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 3 cut(s) 125, 270, 306
PspGI CCWGG 3 cut(s) 125, 270, 306
PspN4I GGNNCC 1 cut(s) 57
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SacI GAGCTC 1 cut(s) 249
SatI GCNGC 2 cut(s) 71, 144
Sau3AI GATC 1 cut(s) 75
ScrFI CCNGG 3 cut(s) 127, 272, 308
SduI GDGCHC 1 cut(s) 249
SetI ASST 8 cut(s) 57, 97, 123, 138, 187, 203, 249, 266
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 1 cut(s) 47
SspMI CTAG 2 cut(s) 66, 278
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 3 cut(s) 125, 270, 306
TaqII GACCGA 1 cut(s) 257
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 2 cut(s) 70, 143
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 217, 267
TspGWI ACGGA 2 cut(s) 48, 123
XmiI GTMKAC 1 cut(s) 284
XspI CTAG 2 cut(s) 66, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.