pycom08g08380

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000732
Physical Location & Seq
Forward (+)
6722473 .. 6722811
339 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08380.1

Sequence Viewer

Length: 339 bp
ATGAGGTGGATAAATGATGTGGATCAGCCGGAAGTTCCCTCCACGGAGCCAAGGGTGGTTGATCATCCCTTTAGGCCGGATCACCATAAGAAGGCCAGAATGCCCTACACAGAGGAGCAGATAAGGAAGGTCTTCAAAAGCTTCGACAAGAACAGAGACGGCCAACTTTCCAAGGCTGAGCCCGAGGCAGCTTTCGCGAAACTCGGCGCGATATGGACCAGCGTTAGAGCTTGGTTAAGTCTTTGGCATGCAGATGACAACGGCGACGACTTCATTTCTCTAGACAACGAGCTGGACAAGCTTGTCGACTATGCCCTAAAACTTGGGTATACCCTTTAG

Protein Analysis

113

Amino Acids

13.04

Weight (kDa)

5.5

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccI GTMKAC 2 cut(s) 306, 329
AccII CGCG 2 cut(s) 197, 209
AclWI GGATC 2 cut(s) 30, 87
AcoI YGGCCR 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 1 cut(s) 136
AluBI AGCT 5 cut(s) 141, 191, 230, 292, 301
AluI AGCT 5 cut(s) 141, 191, 230, 292, 301
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 2 cut(s) 30, 87
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 74, 93, 160
ApeKI GCWGC 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 200
BceAI ACGGC 2 cut(s) 175, 277
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 281
BisI GCNGC 1 cut(s) 189
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 216
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 124
Bpu1102I GCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 21
BsaJI CCNNGG 4 cut(s) 42, 50, 171, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 21
BseDI CCNNGG 4 cut(s) 42, 50, 171, 183
BseGI GGATG 1 cut(s) 64
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 200
Bsh1236I CGCG 2 cut(s) 197, 209
BshFI GGCC 3 cut(s) 76, 95, 162
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 29, 77
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 150
BsmBI CGTCTC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 3 cut(s) 76, 95, 162
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 22, 61, 79
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp68I TCGCGA 1 cut(s) 197
BspANI GGCC 3 cut(s) 76, 95, 162
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 2 cut(s) 197, 209
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 2 cut(s) 30, 87
BssECI CCNNGG 4 cut(s) 42, 50, 171, 183
BssMI GATC 3 cut(s) 22, 61, 79
BssNAI GTATAC 1 cut(s) 330
BssT1I CCWWGG 2 cut(s) 50, 171
Bst1107I GTATAC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 177
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 64
BstFNI CGCG 2 cut(s) 197, 209
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 3 cut(s) 25, 64, 82
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 3 cut(s) 22, 61, 79
BstMWI GCNNNNNNNGC 2 cut(s) 194, 298
BstNSI RCATGY 1 cut(s) 251
BstUI CGCG 2 cut(s) 197, 209
BstV1I GCAGC 1 cut(s) 200
BstV2I GAAGAC 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 330
BsuRI GGCC 3 cut(s) 76, 95, 162
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 64
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 1 cut(s) 249
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 216
CviAII CATG 1 cut(s) 248
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 24, 63, 81
DpnII GATC 3 cut(s) 22, 61, 79
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 50, 171
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 182
EcoT14I CCWWGG 2 cut(s) 50, 171
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 2 cut(s) 50, 171
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 1 cut(s) 251
FaiI YATR 5 cut(s) 87, 214, 249, 312, 330
FatI CATG 1 cut(s) 247
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 2 cut(s) 306, 329
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 1 cut(s) 51
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 208
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 3 cut(s) 76, 95, 162
HapII CCGG 2 cut(s) 29, 77
HhaI GCGC 1 cut(s) 209
Hin1II CATG 1 cut(s) 251
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 2 cut(s) 139, 299
HpaII CCGG 2 cut(s) 29, 77
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 307, 330
Hpy188III TCNNGA 2 cut(s) 196, 281
Hpy8I GTNNAC 2 cut(s) 307, 330
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 85, 121
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 298
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 1 cut(s) 251
HspAI GCGC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 22, 61, 79
LmnI GCTCC 2 cut(s) 46, 115
LpnPI CCDG 5 cut(s) 42, 90, 109, 232, 278
Lsp1109I GCAGC 1 cut(s) 200
MaeI CTAG 1 cut(s) 281
MalI GATC 3 cut(s) 24, 63, 81
MboI GATC 3 cut(s) 22, 61, 79
MboII GAAGA 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 183
MnlI CCTC 3 cut(s) 49, 106, 178
MroXI GAANNNNTTC 1 cut(s) 131
MseI TTAA 1 cut(s) 236
MslI CAYNNNNRTG 1 cut(s) 252
MspI CCGG 2 cut(s) 29, 77
Mva1269I GAATGC 1 cut(s) 105
MvnI CGCG 2 cut(s) 197, 209
MwoI GCNNNNNNNGC 2 cut(s) 194, 298
NdeII GATC 3 cut(s) 22, 61, 79
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 183
NruI TCGCGA 1 cut(s) 197
NspI RCATGY 1 cut(s) 251
PaeI GCATGC 1 cut(s) 251
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 131
PkrI GCNGC 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 216
RruI TCGCGA 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 252
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 3 cut(s) 22, 61, 79
Sau96I GGNCC 1 cut(s) 216
SduI GDGCHC 1 cut(s) 183
SetI ASST 7 cut(s) 8, 132, 143, 193, 232, 294, 303
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 252
SphI GCATGC 1 cut(s) 251
SspMI CTAG 1 cut(s) 281
StyI CCWWGG 2 cut(s) 50, 171
TaqI TCGA 2 cut(s) 144, 306
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 262
TspGWI ACGGA 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 216
XbaI TCTAGA 1 cut(s) 280
XceI RCATGY 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 306, 329
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.