Rh6BG480600

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000732
Physical Location & Seq
Reverse (-)
68654806 .. 68655635
830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG480600.1

Sequence Viewer

Length: 285 bp
ATGGGGCGGAGAGACAACCCGAAGGATGTGTATAAGGGTCCTGTTCCGTACACTAGGCAGCAGATCATAGATATTTTCAAAGGTTTTGACAAGAACGGAGACGGCAAGCTCTCCTGGGATGAGGTGAAGGCTGCGTTCGCTAAACTCGGGGCGCTTTTGCCCGACTACAGAGCTTGGCGAGGACGAAGGTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCTGGAACTAGAGTATACACCGAAGTAA

Protein Analysis

94

Amino Acids

10.79

Weight (kDa)

6.25

Isoelectric Point (pI)

14.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 15 - 46 7.7e-06 EF-hand domain pair
EF-hand_1 PF00036 22 - 49 3.2e-07 EF hand domain
EF-hand_6 PF13405 22 - 50 9.8e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 272
AciI CCGC 1 cut(s) 7
AfaI GTAC 1 cut(s) 50
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 2 cut(s) 113, 258
AluBI AGCT 3 cut(s) 109, 173, 235
AluI AGCT 3 cut(s) 109, 173, 235
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 6, 93, 221
Ama87I CYCGRG 2 cut(s) 146, 224
ApeKI GCWGC 2 cut(s) 58, 131
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 136, 241
AvaI CYCGRG 2 cut(s) 146, 224
AvaII GGWCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 70, 118
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 2 cut(s) 115, 260
BcoDI GTCTC 3 cut(s) 6, 93, 221
BfaI CTAG 2 cut(s) 54, 266
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 155
BisI GCNGC 2 cut(s) 59, 132
BlsI GCNGC 2 cut(s) 60, 133
Bme1390I CCNGG 2 cut(s) 115, 260
Bme18I GGWCC 1 cut(s) 38
BmeT110I CYCGRG 2 cut(s) 146, 224
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 115, 260
BmsI GCATC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 114, 258
BseBI CCWGG 2 cut(s) 115, 260
BseDI CCNNGG 2 cut(s) 114, 258
BseGI GGATG 3 cut(s) 31, 124, 214
BseXI GCAGC 2 cut(s) 70, 118
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 146, 224
BsmAI GTCTC 3 cut(s) 6, 93, 221
BsmBI CGTCTC 1 cut(s) 93
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 2 cut(s) 146, 224
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 114, 258
BssMI GATC 1 cut(s) 63
BssNAI GTATAC 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 115, 260
BstC8I GCNNGC 1 cut(s) 107
BstF5I GGATG 3 cut(s) 31, 124, 214
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 3 cut(s) 6, 93, 221
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 2 cut(s) 115, 260
BstSCI CCNGG 2 cut(s) 113, 258
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 70, 118
BstZ17I GTATAC 1 cut(s) 273
BtsCI GGATG 3 cut(s) 31, 124, 214
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 49
CviJI RGCY 5 cut(s) 109, 131, 173, 213, 235
CviKI_1 RGCY 5 cut(s) 109, 131, 173, 213, 235
CviQI GTAC 1 cut(s) 49
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
EciI GGCGGA 1 cut(s) 22
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 2 cut(s) 146, 224
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 113, 258
EcoT38I GRGCYC 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 93
FaiI YATR 4 cut(s) 33, 68, 255, 273
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 59, 132
FokI GGATG 3 cut(s) 38, 131, 221
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 59, 132
FspBI CTAG 2 cut(s) 54, 266
GlaI GCGC 1 cut(s) 153
GluI GCNGC 2 cut(s) 59, 132
HaeII RGCGCY 1 cut(s) 155
HhaI GCGC 1 cut(s) 154
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HphI GGTGA 2 cut(s) 136, 241
Hpy166II GTNNAC 2 cut(s) 51, 273
Hpy188III TCNNGA 2 cut(s) 224, 226
Hpy8I GTNNAC 2 cut(s) 51, 273
HpyAV CCTTC 3 cut(s) 16, 121, 180
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HspAI GCGC 1 cut(s) 152
Kzo9I GATC 1 cut(s) 63
LpnPI CCDG 5 cut(s) 54, 100, 127, 245, 272
Lsp1109I GCAGC 2 cut(s) 70, 118
LweI GCATC 1 cut(s) 187
MaeI CTAG 2 cut(s) 54, 266
MaeIII GTNAC 1 cut(s) 247
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 237
MnlI CCTC 2 cut(s) 115, 173
MspR9I CCNGG 2 cut(s) 115, 260
MvaI CCWGG 2 cut(s) 115, 260
MwoI GCNNNNNNNGC 1 cut(s) 137
NdeII GATC 1 cut(s) 63
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 247
PaeR7I CTCGAG 1 cut(s) 224
PkrI GCNGC 2 cut(s) 60, 133
PpuMI RGGWCCY 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 113, 258
PspGI CCWGG 2 cut(s) 113, 258
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 38
PspPPI RGGWCCY 1 cut(s) 38
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 1 cut(s) 237
SatI GCNGC 2 cut(s) 59, 132
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 38
ScrFI CCNGG 2 cut(s) 115, 260
SduI GDGCHC 1 cut(s) 237
SetI ASST 7 cut(s) 85, 111, 126, 175, 191, 237, 254
SfaNI GCATC 1 cut(s) 187
SfcI CTRYAG 1 cut(s) 166
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SsiI CCGC 1 cut(s) 7
SspMI CTAG 2 cut(s) 54, 266
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 113, 258
TaqI TCGA 1 cut(s) 225
TaqII GACCGA 1 cut(s) 245
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 2 cut(s) 58, 131
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 205, 255
TspGWI ACGGA 2 cut(s) 36, 111
VpaK11BI GGWCC 1 cut(s) 38
XhoI CTCGAG 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 2 cut(s) 54, 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.