Rroxscaffold_6G00393410

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000732
Physical Location & Seq
Forward (+)
13491645 .. 13492056
412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393410.1

Sequence Viewer

Length: 357 bp
ATGCGAAACGACCACGTTGTGTTACCCGTTGTGATACCTAAGGGTGTTCCGTACATTAGGCAGCAGATCATAGATATTTTCAAAGGTTTTGACAAGAACGGAGACGGCAAGCTCTCCTGGGATGAGGTGCAGGCTGCGTTCGCTAAACTCGGGTCGCATTGTACCTACTATAGAGCTTGGCGAGGACGACGCTGTGCCGATGCCGACAACGATGGCTTCATCTCTCTTGAGAGCGAGCTCGATCAACTTGTCACCTATACCTTGGAACAAAATTATAAACCGAAATTCGGTACGTCTTATATGTGTACGTGTATATGTATGTGTAGACGCATGAGCGTCCTGGTGTGTAAGCGGTAA

Protein Analysis

118

Amino Acids

13.6

Weight (kDa)

8.7

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 23 - 50 7.6e-07 EF hand domain
EF-hand_6 PF13405 23 - 51 2e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AccI GTMKAC 1 cut(s) 325
AciI CCGC 1 cut(s) 352
AcsI RAATTY 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 19
AfaI GTAC 4 cut(s) 53, 163, 292, 307
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 2 cut(s) 116, 339
AluBI AGCT 3 cut(s) 112, 176, 238
AluI AGCT 3 cut(s) 112, 176, 238
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 96
Ama87I CYCGRG 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 61, 134
ApoI RAATTY 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 149
AxyI CCTNAGG 1 cut(s) 39
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 73, 121
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 118, 341
BcoDI GTCTC 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 2 cut(s) 62, 135
BlsI GCNGC 2 cut(s) 63, 136
Bme1390I CCNGG 2 cut(s) 118, 341
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 118, 341
BmsI GCATC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 117, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse21I CCTNAGG 1 cut(s) 39
BseBI CCWGG 2 cut(s) 118, 341
BseDI CCNNGG 2 cut(s) 117, 261
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 1 cut(s) 287
BseXI GCAGC 2 cut(s) 73, 121
BsgI GTGCAG 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 2 cut(s) 66, 241
BspACI CCGC 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 117, 261
BssMI GATC 2 cut(s) 66, 241
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 118, 341
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 110, 132, 236
BstDEI CTNAG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 2 cut(s) 69, 244
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 2 cut(s) 66, 241
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 2 cut(s) 118, 341
BstSCI CCNGG 2 cut(s) 116, 339
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 73, 121
Bsu36I CCTNAGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 127
Cac8I GCNNGC 3 cut(s) 110, 132, 236
CseI GACGC 3 cut(s) 198, 325, 336
Csp6I GTAC 4 cut(s) 52, 162, 291, 306
CviAII CATG 1 cut(s) 331
CviJI RGCY 5 cut(s) 112, 134, 176, 216, 238
CviKI_1 RGCY 5 cut(s) 112, 134, 176, 216, 238
CviQI GTAC 4 cut(s) 52, 162, 291, 306
DdeI CTNAG 1 cut(s) 39
DpnI GATC 2 cut(s) 68, 243
DpnII GATC 2 cut(s) 66, 241
DraIII CACNNNGTG 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 238
Eco81I CCTNAGG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 116, 339
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 1 cut(s) 334
FatI CATG 1 cut(s) 330
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 2 cut(s) 62, 135
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 62, 135
GluI GCNGC 2 cut(s) 62, 135
HgaI GACGC 3 cut(s) 198, 325, 336
Hin1II CATG 1 cut(s) 334
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 2 cut(s) 306, 326
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 2 cut(s) 306, 326
Hpy99I CGWCG 1 cut(s) 192
HpyCH4IV ACGT 3 cut(s) 15, 293, 308
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 39
HpySE526I ACGT 3 cut(s) 15, 293, 308
Hsp92II CATG 1 cut(s) 334
Kzo9I GATC 2 cut(s) 66, 241
LpnPI CCDG 5 cut(s) 103, 116, 130, 326, 353
Lsp1109I GCAGC 2 cut(s) 73, 121
LweI GCATC 1 cut(s) 190
MaeII ACGT 3 cut(s) 15, 293, 308
MaeIII GTNAC 2 cut(s) 21, 250
MalI GATC 2 cut(s) 68, 243
MboI GATC 2 cut(s) 66, 241
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 271, 284
MnlI CCTC 2 cut(s) 118, 176
MspR9I CCNGG 2 cut(s) 118, 341
MvaI CCWGG 2 cut(s) 118, 341
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 66, 241
NlaIII CATG 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 250
PkrI GCNGC 2 cut(s) 63, 136
Ppu21I YACGTR 1 cut(s) 309
PsiI TTATAA 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 116, 339
PspGI CCWGG 2 cut(s) 116, 339
RsaI GTAC 4 cut(s) 53, 163, 292, 307
RsaNI GTAC 4 cut(s) 52, 162, 291, 306
SacI GAGCTC 1 cut(s) 240
SatI GCNGC 2 cut(s) 62, 135
Sau3AI GATC 2 cut(s) 66, 241
ScrFI CCNGG 2 cut(s) 118, 341
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 1 cut(s) 190
SfcI CTRYAG 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 271, 284
SsiI CCGC 1 cut(s) 352
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 116, 339
StyI CCWWGG 1 cut(s) 261
TaiI ACGT 3 cut(s) 18, 296, 311
TaqI TCGA 1 cut(s) 240
TasI AATT 2 cut(s) 271, 284
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 61, 134
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 2 cut(s) 39, 114
XapI RAATTY 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.