Rroxscaffold_5G00373780

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000732
Physical Location & Seq
Reverse (-)
54906094 .. 54909718
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373780.1

Sequence Viewer

Length: 354 bp
ATGGCAATTTCAATTTGGGGATGCATCTTTGGTTGCATCGCGACTAGATATATGCCTCCGCGGAAAGATTGTAAGATGAGGCACACAGCTATTGAGCATAAAGATCCTGATGTTCCATACACTGAGAAGCAGATCATAAATATTTTCAAGAACTTTGACCTGAACGGAGACGGCAAGCTGTCCTGGGATGAGGTGAAGGCTGCGTTTGCTAAACTTGGGGCAAACTTTCCCGACTACAGAGCTTGGCGAGGACGAATGTGTGCCGATGCCGACAAGGATGGCTTCATCTCTCTCGAGACCGAGCTCAATGAACTTGTCACCTATACCCTAAAACAAAGGTATAAACCAAAGTAA

Protein Analysis

117

Amino Acids

13.54

Weight (kDa)

8.67

Isoelectric Point (pI)

23.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 35 - 69 3e-06 EF-hand domain pair
EF-hand_7 PF13499 42 - 99 1.2e-07 EF-hand domain pair
EF-hand_1 PF00036 45 - 72 5e-07 EF hand domain
EF-hand_6 PF13405 45 - 73 1e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 61
AciI CCGC 2 cut(s) 59, 61
AclWI GGATC 1 cut(s) 98
AgsI TTSAA 2 cut(s) 12, 148
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 4 cut(s) 89, 178, 242, 304
AluI AGCT 4 cut(s) 89, 178, 242, 304
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 2 cut(s) 162, 290
AlwI GGATC 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 205, 310
AvaI CYCGRG 1 cut(s) 293
BanII GRGCYC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 272
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 162, 290
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 4 cut(s) 11, 33, 45, 256
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 59, 183
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 59, 183
BseGI GGATG 3 cut(s) 26, 193, 283
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 187
Bsh1236I CGCG 2 cut(s) 41, 61
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 293
BsmAI GTCTC 2 cut(s) 162, 290
BsmBI CGTCTC 1 cut(s) 162
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 2 cut(s) 103, 132
Bsp68I TCGCGA 1 cut(s) 41
BspACI CCGC 2 cut(s) 59, 61
BspCNI CTCAG 1 cut(s) 115
BspFNI CGCG 2 cut(s) 41, 61
BspPI GGATC 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 59, 183
BssMI GATC 2 cut(s) 103, 132
Bst2UI CCWGG 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 26, 193, 283
BstFNI CGCG 2 cut(s) 41, 61
BstKTI GATC 2 cut(s) 106, 135
BstMAI GTCTC 2 cut(s) 162, 290
BstMBI GATC 2 cut(s) 103, 132
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 2 cut(s) 41, 61
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BtgI CCRYGG 1 cut(s) 59
BtgZI GCGATG 1 cut(s) 22
BtsCI GGATG 3 cut(s) 26, 193, 283
BtsIMutI CAGTG 1 cut(s) 120
BtuMI TCGCGA 1 cut(s) 41
Cac8I GCNNGC 1 cut(s) 176
Cfr42I CCGCGG 1 cut(s) 62
CviJI RGCY 6 cut(s) 89, 178, 200, 242, 282, 304
CviKI_1 RGCY 6 cut(s) 89, 178, 200, 242, 282, 304
DdeI CTNAG 1 cut(s) 123
DpnI GATC 2 cut(s) 105, 134
DpnII GATC 2 cut(s) 103, 132
Ecl136II GAGCTC 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 182
EcoT22I ATGCAT 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 162
FaiI YATR 7 cut(s) 51, 53, 99, 118, 137, 324, 342
FalI AAGNNNNNCTT 2 cut(s) 266, 298
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 3 cut(s) 33, 200, 290
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 1 cut(s) 45
GluI GCNGC 1 cut(s) 201
HphI GGTGA 2 cut(s) 205, 310
Hpy188III TCNNGA 6 cut(s) 40, 107, 148, 230, 293, 295
HpyAV CCTTC 1 cut(s) 190
HpyCH4V TGCA 2 cut(s) 24, 36
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 123
KspI CCGCGG 1 cut(s) 62
Kzo9I GATC 2 cut(s) 103, 132
LpnPI CCDG 4 cut(s) 120, 169, 173, 196
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 4 cut(s) 11, 33, 45, 256
MaeI CTAG 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 316
MalI GATC 2 cut(s) 105, 134
MboI GATC 2 cut(s) 103, 132
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 2 cut(s) 6, 12
MnlI CCTC 4 cut(s) 66, 72, 184, 242
Mph1103I ATGCAT 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 61
MspR9I CCNGG 1 cut(s) 184
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 2 cut(s) 41, 61
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeII GATC 2 cut(s) 103, 132
NmuCI GTSAC 1 cut(s) 316
NruI TCGCGA 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 293
PkrI GCNGC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 103
RruI TCGCGA 1 cut(s) 41
SacI GAGCTC 1 cut(s) 306
SacII CCGCGG 1 cut(s) 62
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 2 cut(s) 103, 132
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 1 cut(s) 306
SetI ASST 8 cut(s) 91, 162, 180, 195, 244, 306, 323, 341
SfaNI GCATC 4 cut(s) 11, 33, 45, 256
SfcI CTRYAG 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 62
SgrBI CCGCGG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 2 cut(s) 6, 12
SsiI CCGC 2 cut(s) 59, 61
SspI AATATT 1 cut(s) 142
SspMI CTAG 1 cut(s) 45
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 182
TaqI TCGA 1 cut(s) 294
TaqII GACCGA 1 cut(s) 314
TasI AATT 2 cut(s) 6, 12
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 274, 324
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 293
XspI CTAG 1 cut(s) 45
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.