RchiOBHm_Chr3g0490571

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000732
Physical Location & Seq
Reverse (-)
37215208 .. 37216080
873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45367

Sequence Viewer

Length: 312 bp
ATGCCTTCGGTTACGCGAAACACCGTTGTGTTACCTACGCAAAACCCCGTTGTGTTACCTAAGGGTGGTGTTCCGTACATTAGGCAGCAGATCATAGATATTTTCAAAGGTTTTGACAAGAACGGAGACGGCAAGCTGTCCTGGGATGAGGTGCAGGCTGCGTTCGCTAAACTCGGGTCGCATTTTACCTACTATAGAGCTTGGCGAGGACGACGCTGTGCCGATGCCAACAACGATGGCTTCATCTCTCTTGAGAGCGAGCTCAATGAACTTGTCACCTATACCTTGGAACAAAATTATATCGCGAAGTAA

Protein Analysis

103

Amino Acids

11.65

Weight (kDa)

7.84

Isoelectric Point (pI)

26.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 22 - 55 1e-05 EF-hand domain pair
EF-hand_1 PF00036 31 - 58 5.9e-07 EF hand domain
EF-hand_6 PF13405 31 - 59 1.5e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 16, 305
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 65
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 3 cut(s) 136, 200, 262
AluI AGCT 3 cut(s) 136, 200, 262
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 173
ApeKI GCWGC 2 cut(s) 85, 158
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 173
AxyI CCTNAGG 1 cut(s) 60
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 97, 145
BccI CCATC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 193
BisI GCNGC 2 cut(s) 86, 159
BlsI GCNGC 2 cut(s) 87, 160
Bme1390I CCNGG 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 141, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse21I CCTNAGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 141, 285
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 97, 145
BsgI GTGCAG 1 cut(s) 173
Bsh1236I CGCG 2 cut(s) 16, 305
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 120
BsmBI CGTCTC 1 cut(s) 120
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 1 cut(s) 90
Bsp68I TCGCGA 1 cut(s) 305
BspFNI CGCG 2 cut(s) 16, 305
BssECI CCNNGG 2 cut(s) 141, 285
BssMI GATC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 134, 156, 260
BstDEI CTNAG 1 cut(s) 60
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 2 cut(s) 16, 305
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 193
BstUI CGCG 2 cut(s) 16, 305
BstV1I GCAGC 2 cut(s) 97, 145
Bsu36I CCTNAGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 151
BtuMI TCGCGA 1 cut(s) 305
Cac8I GCNNGC 3 cut(s) 134, 156, 260
CseI GACGC 1 cut(s) 222
Csp6I GTAC 1 cut(s) 76
CviJI RGCY 5 cut(s) 136, 158, 200, 240, 262
CviKI_1 RGCY 5 cut(s) 136, 158, 200, 240, 262
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 120
FaiI YATR 4 cut(s) 95, 195, 282, 300
Fnu4HI GCNGC 2 cut(s) 86, 159
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 2 cut(s) 86, 159
GluI GCNGC 2 cut(s) 86, 159
HgaI GACGC 1 cut(s) 222
HphI GGTGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 251, 304
Hpy99I CGWCG 1 cut(s) 216
HpyAV CCTTC 1 cut(s) 15
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 60
Kzo9I GATC 1 cut(s) 90
LpnPI CCDG 3 cut(s) 127, 140, 154
Lsp1109I GCAGC 2 cut(s) 97, 145
LweI GCATC 1 cut(s) 214
MaeIII GTNAC 4 cut(s) 10, 30, 54, 274
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 1 cut(s) 295
MnlI CCTC 2 cut(s) 142, 200
MslI CAYNNNNRTG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 2 cut(s) 16, 305
MwoI GCNNNNNNNGC 1 cut(s) 164
NdeII GATC 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 274
NruI TCGCGA 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 26
PkrI GCNGC 2 cut(s) 87, 160
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
RruI TCGCGA 1 cut(s) 305
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 26
SacI GAGCTC 1 cut(s) 264
SatI GCNGC 2 cut(s) 86, 159
Sau3AI GATC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 1 cut(s) 295
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 25
TasI AATT 1 cut(s) 295
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 85, 158
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 232, 282
TspGWI ACGGA 2 cut(s) 63, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.