FvH4_2g34530

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000732
Physical Location & Seq
Forward (+)
25629478 .. 25630118
641 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34530.t1

Sequence Viewer

Length: 294 bp
ATGCCTCCGAGAAGACCCCGAGTCACTACGGTGATTGAGAATAAGAAAGTCGATGTTCCGTACACTAAGCAGCAGATCGAAGATATTTTCAAAAGCTATGACCAGAACGGAGACGGCAAGCTCTCCTGGGACGAGGTGAAGGCAGCGTTCGCTAAACTCGGGGCGTTTTCGCCCTACTTCAGAGCCTGGCGAGGGAAAAGCCGTGCCGATGCCAACGAAGACGGCTTCATCTGTCTTCAGACCGAGCTCGATGAACTTGTCAACTATACTCTGGAACTACAGTATAAACTGTAG

Protein Analysis

98

Amino Acids

11.26

Weight (kDa)

6.26

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 16 - 50 2.4e-07 EF-hand domain pair
EF-hand_6 PF13405 25 - 54 6.2e-08 EF-hand domain
EF-hand_1 PF00036 26 - 53 1.5e-07 EF hand domain
EF-hand_5 PF13202 26 - 48 9.9e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 2 cut(s) 163, 221
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 1 cut(s) 91
AhdI GACNNNNNGTC 1 cut(s) 20
AjnI CCWGG 2 cut(s) 125, 185
AleI CACNNNNGTG 1 cut(s) 29
AluBI AGCT 3 cut(s) 96, 121, 247
AluI AGCT 3 cut(s) 96, 121, 247
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 105
AlwNI CAGNNNCTG 1 cut(s) 186
Ama87I CYCGRG 2 cut(s) 18, 158
ApeKI GCWGC 2 cut(s) 70, 143
AsuHPI GGTGA 2 cut(s) 43, 148
AvaI CYCGRG 2 cut(s) 18, 158
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 3 cut(s) 19, 225, 227
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 82, 155
BceAI ACGGC 3 cut(s) 130, 186, 238
BciT130I CCWGG 2 cut(s) 127, 187
BcoDI GTCTC 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 278, 290
BisI GCNGC 2 cut(s) 71, 144
BlsI GCNGC 2 cut(s) 72, 145
Bme1390I CCNGG 2 cut(s) 127, 187
BmeRI GACNNNNNGTC 1 cut(s) 20
BmeT110I CYCGRG 2 cut(s) 18, 158
BmrFI CCNGG 2 cut(s) 127, 187
BmsI GCATC 1 cut(s) 199
BpiI GAAGAC 3 cut(s) 19, 225, 227
BsaJI CCNNGG 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 127, 187
BseDI CCNNGG 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 192
BseXI GCAGC 2 cut(s) 82, 155
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 18, 158
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 105
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 143
BsoBI CYCGRG 2 cut(s) 18, 158
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 127, 187
Bst4CI ACNGT 3 cut(s) 31, 282, 291
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 66
BstKTI GATC 1 cut(s) 78
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 2 cut(s) 127, 187
BstSCI CCNGG 2 cut(s) 125, 185
BstSFI CTRYAG 2 cut(s) 278, 290
BstV1I GCAGC 2 cut(s) 82, 155
BstV2I GAAGAC 3 cut(s) 19, 225, 227
Cac8I GCNNGC 1 cut(s) 119
CaiI CAGNNNCTG 1 cut(s) 186
Csp6I GTAC 1 cut(s) 61
CviJI RGCY 6 cut(s) 96, 121, 185, 201, 225, 247
CviKI_1 RGCY 6 cut(s) 96, 121, 185, 201, 225, 247
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 66
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
DriI GACNNNNNGTC 1 cut(s) 20
Eam1105I GACNNNNNGTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 163, 221
Eco88I CYCGRG 2 cut(s) 18, 158
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 125, 185
EcoT38I GRGCYC 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 105
FaiI YATR 3 cut(s) 99, 267, 285
FaqI GGGAC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 71, 144
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 71, 144
GluI GCNGC 2 cut(s) 71, 144
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 1 cut(s) 21
HphI GGTGA 2 cut(s) 43, 148
Hpy166II GTNNAC 2 cut(s) 63, 262
Hpy188I TCNGA 3 cut(s) 9, 182, 240
Hpy188III TCNNGA 1 cut(s) 272
Hpy8I GTNNAC 2 cut(s) 63, 262
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 3 cut(s) 31, 282, 291
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 66
Kzo9I GATC 1 cut(s) 75
LpnPI CCDG 6 cut(s) 112, 116, 139, 172, 199, 257
Lsp1109I GCAGC 2 cut(s) 82, 155
LweI GCATC 1 cut(s) 199
MaeIII GTNAC 1 cut(s) 22
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 4 cut(s) 24, 92, 227, 230
MhlI GDGCHC 1 cut(s) 249
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 3 cut(s) 15, 127, 185
MslI CAYNNNNRTG 1 cut(s) 29
MspR9I CCNGG 2 cut(s) 127, 187
MvaI CCWGG 2 cut(s) 127, 187
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 22
OliI CACNNNNGTG 1 cut(s) 29
PkrI GCNGC 2 cut(s) 72, 145
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 125, 185
PspGI CCWGG 2 cut(s) 125, 185
PstNI CAGNNNCTG 1 cut(s) 186
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 29
SacI GAGCTC 1 cut(s) 249
SatI GCNGC 2 cut(s) 71, 144
Sau3AI GATC 1 cut(s) 75
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 2 cut(s) 127, 187
SduI GDGCHC 1 cut(s) 249
SetI ASST 4 cut(s) 98, 123, 138, 249
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 2 cut(s) 278, 290
SmiMI CAYNNNNRTG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 125, 185
TaaI ACNGT 3 cut(s) 31, 282, 291
TaqI TCGA 3 cut(s) 51, 78, 249
TaqII GACCGA 1 cut(s) 257
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 70, 143
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 2 cut(s) 217, 267
TspGWI ACGGA 2 cut(s) 48, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.