calcium ion binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 345, 376 |
| AclWI | GGATC | 1 cut(s) | 232 |
| AfaI | GTAC | 1 cut(s) | 66 |
| AjnI | CCWGG | 1 cut(s) | 248 |
| AluBI | AGCT | 3 cut(s) | 237, 307, 349 |
| AluI | AGCT | 3 cut(s) | 237, 307, 349 |
| Alw21I | GWGCWC | 2 cut(s) | 239, 309 |
| AlwI | GGATC | 1 cut(s) | 232 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 249 |
| AoxI | GGCC | 1 cut(s) | 96 |
| ApeKI | GCWGC | 1 cut(s) | 206 |
| AspLEI | GCGC | 1 cut(s) | 358 |
| AspS9I | GGNCC | 1 cut(s) | 109 |
| AsuC2I | CCSGG | 1 cut(s) | 69 |
| AsuHPI | GGTGA | 1 cut(s) | 185 |
| AvaII | GGWCC | 1 cut(s) | 109 |
| BanII | GRGCYC | 2 cut(s) | 239, 309 |
| Bbv12I | GWGCWC | 2 cut(s) | 239, 309 |
| BbvI | GCAGC | 1 cut(s) | 218 |
| BccI | CCATC | 1 cut(s) | 278 |
| BceAI | ACGGC | 1 cut(s) | 193 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 32, 66 |
| BciT130I | CCWGG | 1 cut(s) | 250 |
| BcnI | CCSGG | 1 cut(s) | 69 |
| BfaI | CTAG | 1 cut(s) | 332 |
| BfoI | RGCGCY | 1 cut(s) | 359 |
| BisI | GCNGC | 1 cut(s) | 207 |
| BlsI | GCNGC | 1 cut(s) | 208 |
| Bme1390I | CCNGG | 2 cut(s) | 69, 250 |
| Bme18I | GGWCC | 1 cut(s) | 109 |
| BmgT120I | GGNCC | 1 cut(s) | 109 |
| BmiI | GGNNCC | 1 cut(s) | 111 |
| BmrFI | CCNGG | 2 cut(s) | 69, 250 |
| BmsI | GCATC | 1 cut(s) | 297 |
| BpmI | CTGGAG | 1 cut(s) | 271 |
| Bpu10I | CCTNAGC | 1 cut(s) | 87 |
| BpuMI | CCSGG | 1 cut(s) | 69 |
| BsaBI | GATNNNNATC | 1 cut(s) | 15 |
| Bse8I | GATNNNNATC | 1 cut(s) | 15 |
| BseBI | CCWGG | 1 cut(s) | 250 |
| BseGI | GGATG | 1 cut(s) | 16 |
| BseJI | GATNNNNATC | 1 cut(s) | 15 |
| BseMII | CTCAG | 1 cut(s) | 78 |
| BseRI | GAGGAG | 1 cut(s) | 317 |
| BseXI | GCAGC | 1 cut(s) | 218 |
| BshFI | GGCC | 1 cut(s) | 98 |
| BsiHKAI | GWGCWC | 2 cut(s) | 239, 309 |
| BsiSI | CCGG | 1 cut(s) | 69 |
| BslFI | GGGAC | 1 cut(s) | 95 |
| BsmFI | GGGAC | 1 cut(s) | 95 |
| BsnI | GGCC | 1 cut(s) | 98 |
| Bsp1286I | GDGCHC | 2 cut(s) | 239, 309 |
| Bsp143I | GATC | 4 cut(s) | 16, 47, 198, 224 |
| BspANI | GGCC | 1 cut(s) | 98 |
| BspCNI | CTCAG | 1 cut(s) | 79 |
| BspLI | GGNNCC | 1 cut(s) | 111 |
| BspPI | GGATC | 1 cut(s) | 232 |
| BssMI | GATC | 4 cut(s) | 16, 47, 198, 224 |
| Bst2UI | CCWGG | 1 cut(s) | 250 |
| Bst4CI | ACNGT | 2 cut(s) | 122, 173 |
| Bst6I | CTCTTC | 1 cut(s) | 189 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 186 |
| BstDEI | CTNAG | 1 cut(s) | 87 |
| BstF5I | GGATG | 1 cut(s) | 16 |
| BstH2I | RGCGCY | 1 cut(s) | 359 |
| BstHHI | GCGC | 1 cut(s) | 358 |
| BstKTI | GATC | 4 cut(s) | 19, 50, 201, 227 |
| BstMBI | GATC | 4 cut(s) | 16, 47, 198, 224 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 186, 254, 355 |
| BstNI | CCWGG | 1 cut(s) | 250 |
| BstSCI | CCNGG | 2 cut(s) | 67, 248 |
| BstV1I | GCAGC | 1 cut(s) | 218 |
| BsuRI | GGCC | 1 cut(s) | 98 |
| BtgZI | GCGATG | 1 cut(s) | 297 |
| BtsCI | GGATG | 1 cut(s) | 16 |
| BtsIMutI | CAGTG | 1 cut(s) | 118 |
| CaiI | CAGNNNCTG | 1 cut(s) | 249 |
| CfoI | GCGC | 1 cut(s) | 358 |
| Cfr13I | GGNCC | 1 cut(s) | 109 |
| Csp6I | GTAC | 1 cut(s) | 65 |
| CviAII | CATG | 2 cut(s) | 73, 266 |
| CviJI | RGCY | 6 cut(s) | 98, 209, 237, 248, 307, 349 |
| CviKI_1 | RGCY | 6 cut(s) | 98, 209, 237, 248, 307, 349 |
| CviQI | GTAC | 1 cut(s) | 65 |
| DdeI | CTNAG | 1 cut(s) | 87 |
| DpnI | GATC | 4 cut(s) | 18, 49, 200, 226 |
| DpnII | GATC | 4 cut(s) | 16, 47, 198, 224 |
| Eam1104I | CTCTTC | 1 cut(s) | 189 |
| EarI | CTCTTC | 1 cut(s) | 189 |
| Ecl136II | GAGCTC | 2 cut(s) | 237, 307 |
| Eco24I | GRGCYC | 2 cut(s) | 239, 309 |
| Eco47I | GGWCC | 1 cut(s) | 109 |
| Eco53kI | GAGCTC | 2 cut(s) | 237, 307 |
| EcoICRI | GAGCTC | 2 cut(s) | 237, 307 |
| EcoO109I | RGGNCCY | 1 cut(s) | 109 |
| EcoRII | CCWGG | 1 cut(s) | 248 |
| EcoT38I | GRGCYC | 2 cut(s) | 239, 309 |
| FaeI | CATG | 2 cut(s) | 76, 269 |
| FaiI | YATR | 8 cut(s) | 74, 267, 327, 345, 363, 365, 367, 376 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 194, 226 |
| FaqI | GGGAC | 1 cut(s) | 95 |
| FatI | CATG | 2 cut(s) | 72, 265 |
| Fnu4HI | GCNGC | 1 cut(s) | 207 |
| FokI | GGATG | 1 cut(s) | 23 |
| FriOI | GRGCYC | 2 cut(s) | 239, 309 |
| Fsp4HI | GCNGC | 1 cut(s) | 207 |
| FspBI | CTAG | 1 cut(s) | 332 |
| GlaI | GCGC | 1 cut(s) | 357 |
| GluI | GCNGC | 1 cut(s) | 207 |
| GsuI | CTGGAG | 1 cut(s) | 271 |
| HaeII | RGCGCY | 1 cut(s) | 359 |
| HaeIII | GGCC | 1 cut(s) | 98 |
| HapII | CCGG | 1 cut(s) | 69 |
| HhaI | GCGC | 1 cut(s) | 358 |
| Hin1II | CATG | 2 cut(s) | 76, 269 |
| Hin6I | GCGC | 1 cut(s) | 356 |
| HinP1I | GCGC | 1 cut(s) | 356 |
| HinfI | GANTC | 1 cut(s) | 272 |
| HpaII | CCGG | 1 cut(s) | 69 |
| HphI | GGTGA | 1 cut(s) | 185 |
| Hpy188I | TCNGA | 3 cut(s) | 31, 216, 224 |
| Hpy99I | CGWCG | 1 cut(s) | 314 |
| HpyAV | CCTTC | 2 cut(s) | 88, 100 |
| HpyCH4III | ACNGT | 2 cut(s) | 122, 173 |
| HpyCH4IV | ACGT | 1 cut(s) | 229 |
| HpyCH4V | TGCA | 2 cut(s) | 189, 269 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 186, 254, 355 |
| HpyF3I | CTNAG | 1 cut(s) | 87 |
| HpySE526I | ACGT | 1 cut(s) | 229 |
| Hsp92II | CATG | 2 cut(s) | 76, 269 |
| HspAI | GCGC | 1 cut(s) | 356 |
| Kzo9I | GATC | 4 cut(s) | 16, 47, 198, 224 |
| LmnI | GCTCC | 3 cut(s) | 133, 234, 304 |
| LpnPI | CCDG | 4 cut(s) | 82, 99, 235, 262 |
| Lsp1109I | GCAGC | 1 cut(s) | 218 |
| LweI | GCATC | 1 cut(s) | 297 |
| MaeI | CTAG | 1 cut(s) | 332 |
| MaeII | ACGT | 1 cut(s) | 229 |
| MaeIII | GTNAC | 1 cut(s) | 173 |
| MalI | GATC | 4 cut(s) | 18, 49, 200, 226 |
| MboI | GATC | 4 cut(s) | 16, 47, 198, 224 |
| MboII | GAAGA | 2 cut(s) | 11, 206 |
| MhlI | GDGCHC | 2 cut(s) | 239, 309 |
| MluCI | AATT | 1 cut(s) | 114 |
| MmeI | TCCRAC | 1 cut(s) | 212 |
| MnlI | CCTC | 1 cut(s) | 295 |
| MseI | TTAA | 1 cut(s) | 102 |
| MslI | CAYNNNNRTG | 2 cut(s) | 364, 366 |
| MspI | CCGG | 1 cut(s) | 69 |
| MspR9I | CCNGG | 2 cut(s) | 69, 250 |
| MvaI | CCWGG | 1 cut(s) | 250 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 186, 254, 355 |
| NciI | CCSGG | 1 cut(s) | 69 |
| NdeII | GATC | 4 cut(s) | 16, 47, 198, 224 |
| NlaIII | CATG | 2 cut(s) | 76, 269 |
| NlaIV | GGNNCC | 1 cut(s) | 111 |
| NmuCI | GTSAC | 1 cut(s) | 173 |
| PfeI | GAWTC | 1 cut(s) | 272 |
| PkrI | GCNGC | 1 cut(s) | 208 |
| PpuMI | RGGWCCY | 1 cut(s) | 109 |
| PsiI | TTATAA | 2 cut(s) | 345, 376 |
| Psp124BI | GAGCTC | 2 cut(s) | 239, 309 |
| Psp5II | RGGWCCY | 1 cut(s) | 109 |
| Psp6I | CCWGG | 1 cut(s) | 248 |
| PspGI | CCWGG | 1 cut(s) | 248 |
| PspN4I | GGNNCC | 1 cut(s) | 111 |
| PspPI | GGNCC | 1 cut(s) | 109 |
| PspPPI | RGGWCCY | 1 cut(s) | 109 |
| PstNI | CAGNNNCTG | 1 cut(s) | 249 |
| RsaI | GTAC | 1 cut(s) | 66 |
| RsaNI | GTAC | 1 cut(s) | 65 |
| RseI | CAYNNNNRTG | 2 cut(s) | 364, 366 |
| SacI | GAGCTC | 2 cut(s) | 239, 309 |
| SaqAI | TTAA | 1 cut(s) | 102 |
| SatI | GCNGC | 1 cut(s) | 207 |
| Sau3AI | GATC | 4 cut(s) | 16, 47, 198, 224 |
| Sau96I | GGNCC | 1 cut(s) | 109 |
| ScrFI | CCNGG | 2 cut(s) | 69, 250 |
| SduI | GDGCHC | 2 cut(s) | 239, 309 |
| SetI | ASST | 7 cut(s) | 80, 111, 232, 239, 309, 318, 351 |
| SfaNI | GCATC | 1 cut(s) | 297 |
| SinI | GGWCC | 1 cut(s) | 109 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 364, 366 |
| Sse9I | AATT | 1 cut(s) | 114 |
| SspMI | CTAG | 1 cut(s) | 332 |
| SstI | GAGCTC | 2 cut(s) | 239, 309 |
| StyD4I | CCNGG | 2 cut(s) | 67, 248 |
| TaaI | ACNGT | 2 cut(s) | 122, 173 |
| TaiI | ACGT | 1 cut(s) | 232 |
| TaqI | TCGA | 3 cut(s) | 15, 42, 309 |
| TasI | AATT | 1 cut(s) | 114 |
| TfiI | GAWTC | 1 cut(s) | 272 |
| Tru1I | TTAA | 1 cut(s) | 102 |
| Tru9I | TTAA | 1 cut(s) | 102 |
| TscAI | CASTG | 1 cut(s) | 125 |
| TseFI | GTSAC | 1 cut(s) | 173 |
| TseI | GCWGC | 1 cut(s) | 206 |
| Tsp45I | GTSAC | 1 cut(s) | 173 |
| TspRI | CASTG | 1 cut(s) | 125 |
| VpaK11BI | GGWCC | 1 cut(s) | 109 |
| XspI | CTAG | 1 cut(s) | 332 |