FvH4_3g28600

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000184
Physical Location & Seq
Reverse (-)
21599389 .. 21600422
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28600.t1

Sequence Viewer

Length: 993 bp
ATGACGACGACTTCGGCGCCACAGCTTCCATGCAAGCTCCGCTTCTTATTCTTCATCCTCTCCATCCTCCTCGACGCCGCTCGCCGCTCCAGCGGCACCGTCAACCGCCGTCTTATGTCCCTCTTCGACTTCAAGGCCTCTCCTTCACTCAAACCCACCAACCGCGTCAAAACCTCCGACGTCATGGTCGACCACTCCCGCAACCTCTGGTTCCGACTCTACGTCCCCACCACCGCCGACCCCGCCACGCTCCCCGTCATAATATTCTTCCACGGCGGCGGTTTTGTCTTCTTCTCCGCCAACTCAAAACCCTACGACGACCTATGCAAGCGCCTCGCTCTCGAGCTCCCCGCCGTCGTCATCTCCGTCAATTACCGCCTCGCCCCAGAGCATCCCTTCCCGGCACAATACGACGACGGGTTTGACGTGCTGAAATTTGTGGATACAACAAACTTTGAGGGCTTCCCTAGTGAACTTGACCTAAGGCGGTGTTTCCTCGCCGGAGATAGCGCCGGCGGCAACGTTGCGCACCACGTGGCAGTTAAGGCCAGTGATCATGAGTTCTCCAAAATGAAGGTTATTGGAGTCATAGGGATACAACCCTTTTTCGGAGGGGAAGAGAGGACGGAATCGGAAACCAAGCTTACAAAGGCTCTGTTAATCACTCTGGAGCGAACTGACTGGTACTGGAAGGCTCTTTTGCCGGAGGGGAAGGGTAGAGACGACCCGGCGGTCAATGTATTCGGGCCACATTCAGATGGTTTTTTGGGGAAGAACTTTCCGGCGACGATGGTTATCGTGGGAGGATTTGATCCTTTACAAGACTGGCAGAGGAGATACTACGAGGGGTTGAAGAAAGCTGGGAAAGAAGCTTACTTGGTCGAGTACCCGAATTCGTTTCATGGGTTTTACTGTTTTCCTGAGCTGAAGGAGTCTACCTTATTGATTGATAGAATGAAGGACTTCATCGAGAAGCAATCAGCTCCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

331

Amino Acids

37.21

Weight (kDa)

7.12

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Say1_Mug180 PF10340 64 - 210 2e-06 Steryl acetyl hydrolase
COesterase PF00135 69 - 128 2.3e-07 Carboxylesterase family
BD-FAE PF20434 73 - 190 7.2e-14 BD-FAE
Abhydrolase_3 PF07859 87 - 304 6.3e-62 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 185, 731
AatII GACGTC 1 cut(s) 183
Acc16I TGCGCA 1 cut(s) 528
AccB1I GGYRCC 2 cut(s) 16, 95
AccBSI CCGCTC 2 cut(s) 80, 87
AccI GTMKAC 2 cut(s) 189, 935
AccII CGCG 1 cut(s) 165
AclI AACGTT 1 cut(s) 522
AclWI GGATC 1 cut(s) 806
AcsI RAATTY 2 cut(s) 434, 892
AcuI CTGAAG 1 cut(s) 947
AcvI CACGTG 1 cut(s) 535
AcyI GRCGYC 3 cut(s) 17, 75, 180
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 2 cut(s) 686, 887
AfiI CCNNNNNNNGG 1 cut(s) 608
AgsI TTSAA 2 cut(s) 133, 853
AhdI GACNNNNNGTC 1 cut(s) 221
AjiI CACGTC 1 cut(s) 427
AluBI AGCT 8 cut(s) 25, 37, 346, 643, 860, 872, 925, 983
AluI AGCT 8 cut(s) 25, 37, 346, 643, 860, 872, 925, 983
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 3 cut(s) 135, 546, 746
ApoI RAATTY 2 cut(s) 434, 892
ArsI GACNNNNNNTTYG 3 cut(s) 27, 404, 436
Asp700I GAANNNNTTC 1 cut(s) 962
AspLEI GCGC 4 cut(s) 19, 333, 512, 529
AspS9I GGNCC 1 cut(s) 746
AsuC2I CCSGG 2 cut(s) 401, 728
AvaI CYCGRG 1 cut(s) 341
AxyI CCTNAGG 1 cut(s) 482
BanI GGYRCC 2 cut(s) 16, 95
BanII GRGCYC 1 cut(s) 348
BbrPI CACGTG 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 348
BccI CCATC 3 cut(s) 71, 752, 784
BceAI ACGGC 3 cut(s) 93, 289, 338
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciVI GTATCC 2 cut(s) 436, 588
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 2 cut(s) 401, 728
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 1 cut(s) 468
BfoI RGCGCY 3 cut(s) 20, 334, 513
BfuI GTATCC 2 cut(s) 436, 588
BisI GCNGC 5 cut(s) 78, 85, 94, 277, 517
BlsI GCNGC 5 cut(s) 79, 86, 95, 278, 518
Bme1390I CCNGG 2 cut(s) 401, 728
BmeRI GACNNNNNGTC 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 341
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 3 cut(s) 18, 97, 212
BmrFI CCNGG 2 cut(s) 401, 728
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 2 cut(s) 73, 689
Bpu10I CCTNAGC 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 401, 728
BsaAI YACGTR 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 3 cut(s) 17, 75, 180
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 4 cut(s) 549, 686, 692, 830
Bse21I CCTNAGG 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 794
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 3 cut(s) 54, 63, 391
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 4 cut(s) 549, 686, 692, 830
BseRI GAGGAG 3 cut(s) 59, 847, 975
BseYI CCCAGC 1 cut(s) 860
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 137, 548, 748
BshNI GGYRCC 2 cut(s) 16, 95
BsiHKAI GWGCWC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 6 cut(s) 401, 501, 513, 704, 728, 782
BslFI GGGAC 2 cut(s) 103, 209
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 1 cut(s) 714
BsmBI CGTCTC 1 cut(s) 714
BsmFI GGGAC 2 cut(s) 103, 209
BsnI GGCC 3 cut(s) 137, 548, 748
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 2 cut(s) 553, 811
BspANI GGCC 3 cut(s) 137, 548, 748
BspCNI CTCAG 1 cut(s) 913
BspFNI CGCG 1 cut(s) 165
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 3 cut(s) 18, 97, 212
BspPI GGATC 1 cut(s) 806
BspT107I GGYRCC 2 cut(s) 16, 95
BsrBI CCGCTC 2 cut(s) 80, 87
BsrFI RCCGGY 1 cut(s) 512
BsrI ACTGG 4 cut(s) 549, 686, 692, 830
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 2 cut(s) 553, 811
BssNI GRCGYC 3 cut(s) 17, 75, 180
Bst4CI ACNGT 2 cut(s) 100, 914
Bst6I CTCTTC 2 cut(s) 128, 612
BstACI GRCGYC 3 cut(s) 17, 75, 180
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 35, 82, 329, 514
BstDEI CTNAG 2 cut(s) 482, 921
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 3 cut(s) 54, 63, 391
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 3 cut(s) 20, 334, 513
BstHHI GCGC 4 cut(s) 19, 333, 512, 529
BstKTI GATC 2 cut(s) 556, 814
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 2 cut(s) 553, 811
BstMWI GCNNNNNNNGC 6 cut(s) 39, 90, 93, 242, 516, 545
BstSCI CCNGG 2 cut(s) 399, 726
BstUI CGCG 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 482
BsuI GTATCC 2 cut(s) 436, 588
BsuRI GGCC 3 cut(s) 137, 548, 748
BtgI CCRYGG 1 cut(s) 271
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 3 cut(s) 54, 63, 391
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 4 cut(s) 35, 82, 329, 514
CciI TCATGA 1 cut(s) 556
CfoI GCGC 4 cut(s) 19, 333, 512, 529
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 746
CseI GACGC 2 cut(s) 83, 154
Csp6I GTAC 2 cut(s) 685, 886
CviAII CATG 4 cut(s) 30, 184, 557, 902
CviQI GTAC 2 cut(s) 685, 886
DdeI CTNAG 2 cut(s) 482, 921
DinI GGCGCC 1 cut(s) 18
DpnI GATC 2 cut(s) 555, 813
DpnII GATC 2 cut(s) 553, 811
DraIII CACNNNGTG 1 cut(s) 535
DrdI GACNNNNNNGTC 2 cut(s) 185, 731
DriI GACNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 2 cut(s) 185, 731
Eam1104I CTCTTC 2 cut(s) 128, 612
Eam1105I GACNNNNNGTC 1 cut(s) 221
EarI CTCTTC 2 cut(s) 128, 612
EciI GGCGGA 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 947
Eco72I CACGTG 1 cut(s) 535
Eco81I CCTNAGG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 714
FaeI CATG 4 cut(s) 33, 187, 560, 905
FaiI YATR 8 cut(s) 31, 116, 185, 260, 325, 558, 590, 903
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 2 cut(s) 103, 209
FatI CATG 4 cut(s) 29, 183, 556, 901
FauI CCCGC 3 cut(s) 206, 250, 358
FbaI TGATCA 1 cut(s) 553
FblI GTMKAC 2 cut(s) 189, 935
Fnu4HI GCNGC 5 cut(s) 78, 85, 94, 277, 517
FokI GGATG 3 cut(s) 41, 50, 378
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 5 cut(s) 78, 85, 94, 277, 517
FspBI CTAG 1 cut(s) 468
FspI TGCGCA 1 cut(s) 528
GlaI GCGC 4 cut(s) 18, 332, 511, 528
GluI GCNGC 5 cut(s) 78, 85, 94, 277, 517
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 2 cut(s) 73, 689
HaeII RGCGCY 3 cut(s) 20, 334, 513
HaeIII GGCC 3 cut(s) 137, 548, 748
HapII CCGG 6 cut(s) 401, 501, 513, 704, 728, 782
HgaI GACGC 2 cut(s) 83, 154
HhaI GCGC 4 cut(s) 19, 333, 512, 529
Hin1I GRCGYC 3 cut(s) 17, 75, 180
Hin1II CATG 4 cut(s) 33, 187, 560, 905
Hin6I GCGC 4 cut(s) 17, 331, 510, 527
HinP1I GCGC 4 cut(s) 17, 331, 510, 527
HincII GTYRAC 2 cut(s) 103, 190
HindII GTYRAC 2 cut(s) 103, 190
HindIII AAGCTT 2 cut(s) 641, 870
HinfI GANTC 4 cut(s) 216, 585, 629, 932
HpaII CCGG 6 cut(s) 401, 501, 513, 704, 728, 782
Hpy166II GTNNAC 4 cut(s) 103, 190, 473, 936
Hpy188I TCNGA 5 cut(s) 178, 215, 611, 634, 757
Hpy188III TCNNGA 5 cut(s) 341, 557, 668, 920, 970
Hpy8I GTNNAC 4 cut(s) 103, 190, 473, 936
Hpy99I CGWCG 8 cut(s) 10, 77, 182, 320, 359, 416, 419, 790
HpyAV CCTTC 7 cut(s) 153, 406, 568, 685, 706, 922, 952
HpyCH4III ACNGT 2 cut(s) 100, 914
HpyCH4IV ACGT 5 cut(s) 180, 222, 426, 522, 534
HpyCH4V TGCA 2 cut(s) 33, 327
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 90, 93, 242, 516, 545
HpyF3I CTNAG 2 cut(s) 482, 921
HpySE526I ACGT 5 cut(s) 180, 222, 426, 522, 534
Hsp92I GRCGYC 3 cut(s) 17, 75, 180
Hsp92II CATG 4 cut(s) 33, 187, 560, 905
HspAI GCGC 4 cut(s) 17, 331, 510, 527
KasI GGCGCC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 2 cut(s) 553, 811
LmnI GCTCC 6 cut(s) 42, 92, 255, 351, 670, 988
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 468
MaeII ACGT 5 cut(s) 180, 222, 426, 522, 534
MalI GATC 2 cut(s) 555, 813
MbiI CCGCTC 2 cut(s) 80, 87
MboI GATC 2 cut(s) 553, 811
MboII GAAGA 8 cut(s) 43, 115, 259, 280, 283, 629, 784, 865
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 3 cut(s) 370, 434, 892
Mly113I GGCGCC 1 cut(s) 17
MlyI GAGTC 3 cut(s) 210, 594, 941
MmeI TCCRAC 2 cut(s) 201, 238
MreI CGCCGGCG 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 962
MseI TTAA 2 cut(s) 543, 659
MslI CAYNNNNRTG 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 6 cut(s) 401, 501, 513, 704, 728, 782
MspR9I CCNGG 2 cut(s) 401, 728
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 6 cut(s) 39, 90, 93, 242, 516, 545
NaeI GCCGGC 1 cut(s) 514
NarI GGCGCC 1 cut(s) 17
NciI CCSGG 2 cut(s) 401, 728
NdeII GATC 2 cut(s) 553, 811
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 4 cut(s) 33, 187, 560, 905
NlaIV GGNNCC 3 cut(s) 18, 97, 212
NsbI TGCGCA 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 341
PagI TCATGA 1 cut(s) 556
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 363, 423
PdiI GCCGGC 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 962
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 5 cut(s) 79, 86, 95, 278, 518
PleI GAGTC 3 cut(s) 210, 593, 940
PluTI GGCGCC 1 cut(s) 20
PmaCI CACGTG 1 cut(s) 535
PmlI CACGTG 1 cut(s) 535
PpsI GAGTC 3 cut(s) 210, 593, 940
Ppu21I YACGTR 1 cut(s) 535
Psp124BI GAGCTC 1 cut(s) 348
Psp1406I AACGTT 1 cut(s) 522
PspCI CACGTG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 860
PspN4I GGNNCC 3 cut(s) 18, 97, 212
PspPI GGNCC 1 cut(s) 746
RsaI GTAC 2 cut(s) 686, 887
RsaNI GTAC 2 cut(s) 685, 886
RseI CAYNNNNRTG 1 cut(s) 756
SacI GAGCTC 1 cut(s) 348
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 543, 659
SatI GCNGC 5 cut(s) 78, 85, 94, 277, 517
Sau3AI GATC 2 cut(s) 553, 811
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 3 cut(s) 210, 594, 941
ScrFI CCNGG 2 cut(s) 401, 728
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 400
SfoI GGCGCC 1 cut(s) 18
Sfr274I CTCGAG 1 cut(s) 341
SgrAI CRCCGGYG 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 370, 434, 892
SseBI AGGCCT 1 cut(s) 137
SspDI GGCGCC 1 cut(s) 16
SspI AATATT 1 cut(s) 264
SspMI CTAG 1 cut(s) 468
SstI GAGCTC 1 cut(s) 348
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 399, 726
TaaI ACNGT 2 cut(s) 100, 914
TaiI ACGT 5 cut(s) 183, 225, 429, 525, 537
TaqI TCGA 6 cut(s) 72, 126, 189, 342, 882, 969
TasI AATT 3 cut(s) 370, 434, 892
TauI GCSGC 5 cut(s) 80, 87, 96, 279, 519
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 2 cut(s) 543, 659
Tru9I TTAA 2 cut(s) 543, 659
TscAI CASTG 1 cut(s) 556
TspDTI ATGAA 5 cut(s) 43, 587, 890, 955, 971
TspGWI ACGGA 2 cut(s) 355, 641
TspRI CASTG 1 cut(s) 556
XapI RAATTY 2 cut(s) 434, 892
XhoI CTCGAG 1 cut(s) 341
XmiI GTMKAC 2 cut(s) 189, 935
XmnI GAANNNNTTC 1 cut(s) 962
XspI CTAG 1 cut(s) 468
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.