Carboxylesterase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 662, 743 |
| AatII | GACGTC | 5 cut(s) | 109, 308, 557, 741, 990 |
| Acc36I | ACCTGC | 1 cut(s) | 860 |
| Acc65I | GGTACC | 1 cut(s) | 486 |
| AccB1I | GGYRCC | 3 cut(s) | 284, 486, 659 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1093, 1282 |
| AccBSI | CCGCTC | 5 cut(s) | 644, 809, 1174, 1296, 1361 |
| AccI | GTMKAC | 4 cut(s) | 633, 690, 747, 1265 |
| AccII | CGCG | 2 cut(s) | 1199, 1307 |
| AccIII | TCCGGA | 1 cut(s) | 946 |
| AclWI | GGATC | 7 cut(s) | 457, 1035, 1183, 1199, 1212, 1358, 1371 |
| AcoI | YGGCCR | 2 cut(s) | 1003, 1056 |
| AcyI | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| AfaI | GTAC | 3 cut(s) | 488, 1101, 1391 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 137, 379, 438, 1060, 1093, 1160, 1191, 1282 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 991 |
| AjnI | CCWGG | 2 cut(s) | 1053, 1080 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1193, 1225 |
| AleI | CACNNNNGTG | 2 cut(s) | 592, 837 |
| AluBI | AGCT | 2 cut(s) | 149, 584 |
| AluI | AGCT | 2 cut(s) | 149, 584 |
| Alw21I | GWGCWC | 1 cut(s) | 954 |
| Alw26I | GTCTC | 3 cut(s) | 269, 680, 944 |
| AlwI | GGATC | 7 cut(s) | 457, 1035, 1183, 1199, 1212, 1358, 1371 |
| Ama87I | CYCGRG | 1 cut(s) | 1269 |
| Aor13HI | TCCGGA | 1 cut(s) | 946 |
| ApeKI | GCWGC | 4 cut(s) | 94, 97, 855, 1369 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 419, 451, 965, 997 |
| Asp718I | GGTACC | 1 cut(s) | 486 |
| AspLEI | GCGC | 1 cut(s) | 1070 |
| AspS9I | GGNCC | 6 cut(s) | 51, 1084, 1176, 1273, 1298, 1489 |
| AsuC2I | CCSGG | 5 cut(s) | 785, 1060, 1135, 1270, 1271 |
| AsuHPI | GGTGA | 4 cut(s) | 400, 696, 718, 1231 |
| AvaI | CYCGRG | 1 cut(s) | 1269 |
| AvaII | GGWCC | 3 cut(s) | 1176, 1273, 1489 |
| AxyI | CCTNAGG | 1 cut(s) | 378 |
| BamHI | GGATCC | 2 cut(s) | 1204, 1363 |
| BanI | GGYRCC | 3 cut(s) | 284, 486, 659 |
| BanII | GRGCYC | 2 cut(s) | 324, 445 |
| BbsI | GAAGAC | 1 cut(s) | 605 |
| Bbv12I | GWGCWC | 1 cut(s) | 954 |
| BbvI | GCAGC | 4 cut(s) | 81, 84, 867, 1356 |
| BccI | CCATC | 1 cut(s) | 577 |
| BceAI | ACGGC | 8 cut(s) | 186, 657, 673, 736, 850, 873, 899, 1001 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 89, 123, 260, 294 |
| BciT130I | CCWGG | 2 cut(s) | 1055, 1082 |
| BciVI | GTATCC | 2 cut(s) | 1341, 1449 |
| BcnI | CCSGG | 5 cut(s) | 785, 1060, 1135, 1270, 1271 |
| BcoDI | GTCTC | 3 cut(s) | 269, 680, 944 |
| BfmI | CTRYAG | 3 cut(s) | 279, 853, 1370 |
| BfoI | RGCGCY | 1 cut(s) | 1071 |
| BfuAI | ACCTGC | 1 cut(s) | 860 |
| BfuI | GTATCC | 2 cut(s) | 1341, 1449 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 657 |
| BisI | GCNGC | 8 cut(s) | 95, 98, 210, 642, 856, 900, 1165, 1370 |
| BlsI | GCNGC | 8 cut(s) | 96, 99, 211, 643, 857, 901, 1166, 1371 |
| Bme1390I | CCNGG | 7 cut(s) | 785, 1055, 1060, 1082, 1135, 1270, 1271 |
| Bme18I | GGWCC | 3 cut(s) | 1176, 1273, 1489 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 991 |
| BmeT110I | CYCGRG | 1 cut(s) | 1269 |
| BmgT120I | GGNCC | 6 cut(s) | 51, 1084, 1176, 1273, 1298, 1489 |
| BmrFI | CCNGG | 7 cut(s) | 785, 1055, 1060, 1082, 1135, 1270, 1271 |
| BmsI | GCATC | 1 cut(s) | 381 |
| BpiI | GAAGAC | 1 cut(s) | 605 |
| BpmI | CTGGAG | 1 cut(s) | 159 |
| BpuMI | CCSGG | 5 cut(s) | 785, 1060, 1135, 1270, 1271 |
| BsaHI | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| BsaJI | CCNNGG | 4 cut(s) | 696, 1000, 1059, 1269 |
| BsaWI | WCCGGW | 2 cut(s) | 557, 946 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 30, 60 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 137, 379, 438, 1060, 1093, 1160, 1191, 1282 |
| Bse118I | RCCGGY | 2 cut(s) | 251, 1255 |
| Bse1I | ACTGG | 4 cut(s) | 176, 1238, 1382, 1500 |
| Bse21I | CCTNAGG | 1 cut(s) | 378 |
| BseAI | TCCGGA | 1 cut(s) | 946 |
| BseBI | CCWGG | 2 cut(s) | 1055, 1082 |
| BseDI | CCNNGG | 4 cut(s) | 696, 1000, 1059, 1269 |
| BseGI | GGATG | 1 cut(s) | 780 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 137, 379, 438, 1060, 1093, 1160, 1191, 1282 |
| BseMII | CTCAG | 4 cut(s) | 94, 329, 369, 1473 |
| BseNI | ACTGG | 4 cut(s) | 176, 1238, 1382, 1500 |
| BseRI | GAGGAG | 5 cut(s) | 721, 853, 1184, 1371, 1399 |
| BseXI | GCAGC | 4 cut(s) | 81, 84, 867, 1356 |
| Bsh1236I | CGCG | 2 cut(s) | 1199, 1307 |
| Bsh1285I | CGRYCG | 4 cut(s) | 142, 406, 789, 1260 |
| BshNI | GGYRCC | 3 cut(s) | 284, 486, 659 |
| BsiEI | CGRYCG | 4 cut(s) | 142, 406, 789, 1260 |
| BsiHKAI | GWGCWC | 1 cut(s) | 954 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1269 |
| BslFI | GGGAC | 3 cut(s) | 114, 1076, 1286 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 137, 379, 438, 1060, 1093, 1160, 1191, 1282 |
| BsmAI | GTCTC | 3 cut(s) | 269, 680, 944 |
| BsmBI | CGTCTC | 2 cut(s) | 680, 944 |
| BsmFI | GGGAC | 3 cut(s) | 114, 1076, 1286 |
| BsoBI | CYCGRG | 1 cut(s) | 1269 |
| Bsp1286I | GDGCHC | 3 cut(s) | 324, 445, 954 |
| Bsp13I | TCCGGA | 1 cut(s) | 946 |
| Bsp143I | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| Bsp68I | TCGCGA | 1 cut(s) | 1199 |
| BspCNI | CTCAG | 4 cut(s) | 93, 330, 370, 1474 |
| BspEI | TCCGGA | 1 cut(s) | 946 |
| BspFNI | CGCG | 2 cut(s) | 1199, 1307 |
| BspMAI | CTGCAG | 3 cut(s) | 283, 857, 1374 |
| BspMI | ACCTGC | 1 cut(s) | 860 |
| BspPI | GGATC | 7 cut(s) | 457, 1035, 1183, 1199, 1212, 1358, 1371 |
| BspQI | GCTCTTC | 2 cut(s) | 782, 816 |
| BspT107I | GGYRCC | 3 cut(s) | 284, 486, 659 |
| BsrBI | CCGCTC | 5 cut(s) | 644, 809, 1174, 1296, 1361 |
| BsrFI | RCCGGY | 2 cut(s) | 251, 1255 |
| BsrI | ACTGG | 4 cut(s) | 176, 1238, 1382, 1500 |
| BssAI | RCCGGY | 2 cut(s) | 251, 1255 |
| BssECI | CCNNGG | 4 cut(s) | 696, 1000, 1059, 1269 |
| BssMI | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| BssNI | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| BssT1I | CCWWGG | 1 cut(s) | 696 |
| Bst2UI | CCWGG | 2 cut(s) | 1055, 1082 |
| Bst4CI | ACNGT | 8 cut(s) | 333, 591, 664, 938, 1123, 1155, 1219, 1342 |
| Bst6I | CTCTTC | 3 cut(s) | 500, 782, 816 |
| BstACI | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| BstC8I | GCNNGC | 5 cut(s) | 283, 609, 897, 1197, 1298 |
| BstDEI | CTNAG | 4 cut(s) | 80, 338, 378, 1482 |
| BstEII | GGTNACC | 1 cut(s) | 1219 |
| BstF5I | GGATG | 1 cut(s) | 780 |
| BstFNI | CGCG | 2 cut(s) | 1199, 1307 |
| BstH2I | RGCGCY | 1 cut(s) | 1071 |
| BstHHI | GCGC | 1 cut(s) | 1070 |
| BstKTI | GATC | 9 cut(s) | 191, 406, 465, 1043, 1050, 1191, 1207, 1366, 1434 |
| BstMAI | GTCTC | 3 cut(s) | 269, 680, 944 |
| BstMBI | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| BstMCI | CGRYCG | 4 cut(s) | 142, 406, 789, 1260 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 657, 878, 1378 |
| BstNI | CCWGG | 2 cut(s) | 1055, 1082 |
| BstPI | GGTNACC | 1 cut(s) | 1219 |
| BstSCI | CCNGG | 7 cut(s) | 783, 1053, 1058, 1080, 1133, 1268, 1269 |
| BstSFI | CTRYAG | 3 cut(s) | 279, 853, 1370 |
| BstUI | CGCG | 2 cut(s) | 1199, 1307 |
| BstV1I | GCAGC | 4 cut(s) | 81, 84, 867, 1356 |
| BstV2I | GAAGAC | 1 cut(s) | 605 |
| BstX2I | RGATCY | 4 cut(s) | 1040, 1188, 1204, 1363 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 576 |
| BstYI | RGATCY | 4 cut(s) | 1040, 1188, 1204, 1363 |
| Bsu36I | CCTNAGG | 1 cut(s) | 378 |
| BsuI | GTATCC | 2 cut(s) | 1341, 1449 |
| BtsCI | GGATG | 1 cut(s) | 780 |
| BtsI | GCAGTG | 2 cut(s) | 227, 237 |
| BtsIMutI | CAGTG | 4 cut(s) | 227, 237, 299, 587 |
| BtuMI | TCGCGA | 1 cut(s) | 1199 |
| BveI | ACCTGC | 1 cut(s) | 860 |
| Cac8I | GCNNGC | 5 cut(s) | 283, 609, 897, 1197, 1298 |
| CfoI | GCGC | 1 cut(s) | 1070 |
| Cfr10I | RCCGGY | 2 cut(s) | 251, 1255 |
| Cfr13I | GGNCC | 6 cut(s) | 51, 1084, 1176, 1273, 1298, 1489 |
| Cfr9I | CCCGGG | 1 cut(s) | 1269 |
| CpoI | CGGWCCG | 1 cut(s) | 1176 |
| CseI | GACGC | 5 cut(s) | 437, 625, 712, 887, 1022 |
| Csp6I | GTAC | 3 cut(s) | 487, 1100, 1390 |
| CspI | CGGWCCG | 1 cut(s) | 1176 |
| CviAII | CATG | 7 cut(s) | 41, 192, 289, 528, 576, 1277, 1454 |
| CviQI | GTAC | 3 cut(s) | 487, 1100, 1390 |
| DdeI | CTNAG | 4 cut(s) | 80, 338, 378, 1482 |
| DpnI | GATC | 9 cut(s) | 190, 405, 464, 1042, 1049, 1190, 1206, 1365, 1433 |
| DpnII | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 662, 743 |
| DriI | GACNNNNNGTC | 1 cut(s) | 991 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 662, 743 |
| EaeI | YGGCCR | 2 cut(s) | 1003, 1056 |
| Eam1104I | CTCTTC | 3 cut(s) | 500, 782, 816 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 991 |
| EarI | CTCTTC | 3 cut(s) | 500, 782, 816 |
| EciI | GGCGGA | 1 cut(s) | 406 |
| Eco130I | CCWWGG | 1 cut(s) | 696 |
| Eco147I | AGGCCT | 2 cut(s) | 343, 701 |
| Eco24I | GRGCYC | 2 cut(s) | 324, 445 |
| Eco47I | GGWCC | 3 cut(s) | 1176, 1273, 1489 |
| Eco81I | CCTNAGG | 1 cut(s) | 378 |
| Eco88I | CYCGRG | 1 cut(s) | 1269 |
| Eco91I | GGTNACC | 1 cut(s) | 1219 |
| EcoO65I | GGTNACC | 1 cut(s) | 1219 |
| EcoRII | CCWGG | 2 cut(s) | 1053, 1080 |
| EcoT14I | CCWWGG | 1 cut(s) | 696 |
| EcoT38I | GRGCYC | 2 cut(s) | 324, 445 |
| ErhI | CCWWGG | 1 cut(s) | 696 |
| Esp3I | CGTCTC | 2 cut(s) | 680, 944 |
| FaeI | CATG | 7 cut(s) | 44, 195, 292, 531, 579, 1280, 1457 |
| FaqI | GGGAC | 3 cut(s) | 114, 1076, 1286 |
| FatI | CATG | 7 cut(s) | 40, 191, 288, 527, 575, 1276, 1453 |
| FauI | CCCGC | 2 cut(s) | 919, 1289 |
| FblI | GTMKAC | 4 cut(s) | 633, 690, 747, 1265 |
| Fnu4HI | GCNGC | 8 cut(s) | 95, 98, 210, 642, 856, 900, 1165, 1370 |
| FokI | GGATG | 1 cut(s) | 767 |
| FriOI | GRGCYC | 2 cut(s) | 324, 445 |
| Fsp4HI | GCNGC | 8 cut(s) | 95, 98, 210, 642, 856, 900, 1165, 1370 |
| GlaI | GCGC | 1 cut(s) | 1069 |
| GluI | GCNGC | 8 cut(s) | 95, 98, 210, 642, 856, 900, 1165, 1370 |
| GsuI | CTGGAG | 1 cut(s) | 159 |
| HaeII | RGCGCY | 1 cut(s) | 1071 |
| HgaI | GACGC | 5 cut(s) | 437, 625, 712, 887, 1022 |
| HhaI | GCGC | 1 cut(s) | 1070 |
| Hin1I | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| Hin1II | CATG | 7 cut(s) | 44, 195, 292, 531, 579, 1280, 1457 |
| Hin6I | GCGC | 1 cut(s) | 1068 |
| HinP1I | GCGC | 1 cut(s) | 1068 |
| HincII | GTYRAC | 7 cut(s) | 615, 634, 667, 691, 748, 931, 1266 |
| HindII | GTYRAC | 7 cut(s) | 615, 634, 667, 691, 748, 931, 1266 |
| HphI | GGTGA | 4 cut(s) | 400, 696, 718, 1231 |
| Hpy166II | GTNNAC | 8 cut(s) | 615, 634, 667, 691, 748, 931, 1266, 1312 |
| Hpy188I | TCNGA | 7 cut(s) | 339, 477, 565, 736, 1119, 1186, 1489 |
| Hpy188III | TCNNGA | 9 cut(s) | 153, 314, 714, 820, 847, 947, 1198, 1472, 1503 |
| Hpy8I | GTNNAC | 8 cut(s) | 615, 634, 667, 691, 748, 931, 1266, 1312 |
| HpyAV | CCTTC | 8 cut(s) | 23, 442, 892, 972, 1006, 1031, 1195, 1237 |
| HpyCH4III | ACNGT | 8 cut(s) | 333, 591, 664, 938, 1123, 1155, 1219, 1342 |
| HpyCH4IV | ACGT | 6 cut(s) | 106, 305, 554, 738, 987, 1290 |
| HpyCH4V | TGCA | 6 cut(s) | 31, 100, 267, 281, 855, 1372 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 657, 878, 1378 |
| HpyF3I | CTNAG | 4 cut(s) | 80, 338, 378, 1482 |
| HpySE526I | ACGT | 6 cut(s) | 106, 305, 554, 738, 987, 1290 |
| Hsp92I | GRCGYC | 9 cut(s) | 106, 305, 429, 554, 723, 738, 879, 987, 1014 |
| Hsp92II | CATG | 7 cut(s) | 44, 195, 292, 531, 579, 1280, 1457 |
| HspAI | GCGC | 1 cut(s) | 1068 |
| Kpn2I | TCCGGA | 1 cut(s) | 946 |
| KpnI | GGTACC | 1 cut(s) | 490 |
| Kzo9I | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| LguI | GCTCTTC | 2 cut(s) | 782, 816 |
| LmnI | GCTCC | 5 cut(s) | 178, 448, 949, 1171, 1358 |
| Lsp1109I | GCAGC | 4 cut(s) | 81, 84, 867, 1356 |
| LweI | GCATC | 1 cut(s) | 381 |
| MaeII | ACGT | 6 cut(s) | 106, 305, 554, 738, 987, 1290 |
| MaeIII | GTNAC | 4 cut(s) | 297, 724, 1213, 1219 |
| MalI | GATC | 9 cut(s) | 190, 405, 464, 1042, 1049, 1190, 1206, 1365, 1433 |
| MbiI | CCGCTC | 5 cut(s) | 644, 809, 1174, 1296, 1361 |
| MboI | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| MboII | GAAGA | 9 cut(s) | 109, 359, 427, 517, 610, 769, 803, 1042, 1150 |
| MflI | RGATCY | 4 cut(s) | 1040, 1188, 1204, 1363 |
| MhlI | GDGCHC | 3 cut(s) | 324, 445, 954 |
| MluCI | AATT | 1 cut(s) | 1396 |
| MlyI | GAGTC | 9 cut(s) | 136, 165, 208, 305, 595, 686, 794, 1190, 1493 |
| MmeI | TCCRAC | 6 cut(s) | 307, 490, 759, 1097, 1174, 1467 |
| MroI | TCCGGA | 1 cut(s) | 946 |
| MseI | TTAA | 3 cut(s) | 224, 456, 1540 |
| MslI | CAYNNNNRTG | 3 cut(s) | 574, 592, 837 |
| MspA1I | CMGCKG | 1 cut(s) | 1369 |
| MspR9I | CCNGG | 7 cut(s) | 785, 1055, 1060, 1082, 1135, 1270, 1271 |
| MvaI | CCWGG | 2 cut(s) | 1055, 1082 |
| MvnI | CGCG | 2 cut(s) | 1199, 1307 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 657, 878, 1378 |
| NciI | CCSGG | 5 cut(s) | 785, 1060, 1135, 1270, 1271 |
| NdeII | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| NlaIII | CATG | 7 cut(s) | 44, 195, 292, 531, 579, 1280, 1457 |
| NmeAIII | GCCGAG | 3 cut(s) | 101, 185, 981 |
| NmuCI | GTSAC | 4 cut(s) | 297, 724, 1213, 1219 |
| NruI | TCGCGA | 1 cut(s) | 1199 |
| OliI | CACNNNNGTG | 2 cut(s) | 592, 837 |
| PceI | AGGCCT | 2 cut(s) | 343, 701 |
| PciSI | GCTCTTC | 2 cut(s) | 782, 816 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 500, 744, 924, 981, 984, 1008 |
| PfeI | GAWTC | 2 cut(s) | 310, 1506 |
| PflFI | GACNNNGTC | 4 cut(s) | 217, 553, 1016, 1180 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1093, 1282 |
| PkrI | GCNGC | 8 cut(s) | 96, 99, 211, 643, 857, 901, 1166, 1371 |
| Ple19I | CGATCG | 1 cut(s) | 406 |
| PleI | GAGTC | 9 cut(s) | 135, 164, 208, 304, 595, 686, 794, 1189, 1492 |
| PpsI | GAGTC | 9 cut(s) | 135, 164, 208, 304, 595, 686, 794, 1189, 1492 |
| Psp6I | CCWGG | 2 cut(s) | 1053, 1080 |
| PspEI | GGTNACC | 1 cut(s) | 1219 |
| PspGI | CCWGG | 2 cut(s) | 1053, 1080 |
| PspPI | GGNCC | 6 cut(s) | 51, 1084, 1176, 1273, 1298, 1489 |
| PstI | CTGCAG | 3 cut(s) | 283, 857, 1374 |
| PsuI | RGATCY | 4 cut(s) | 1040, 1188, 1204, 1363 |
| PsyI | GACNNNGTC | 4 cut(s) | 217, 553, 1016, 1180 |
| PvuI | CGATCG | 1 cut(s) | 406 |
| RruI | TCGCGA | 1 cut(s) | 1199 |
| RsaI | GTAC | 3 cut(s) | 488, 1101, 1391 |
| RsaNI | GTAC | 3 cut(s) | 487, 1100, 1390 |
| RseI | CAYNNNNRTG | 3 cut(s) | 574, 592, 837 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1176 |
| RsrII | CGGWCCG | 1 cut(s) | 1176 |
| SalI | GTCGAC | 4 cut(s) | 632, 689, 746, 1264 |
| SapI | GCTCTTC | 2 cut(s) | 782, 816 |
| SaqAI | TTAA | 3 cut(s) | 224, 456, 1540 |
| SatI | GCNGC | 8 cut(s) | 95, 98, 210, 642, 856, 900, 1165, 1370 |
| Sau3AI | GATC | 9 cut(s) | 188, 403, 462, 1040, 1047, 1188, 1204, 1363, 1431 |
| Sau96I | GGNCC | 6 cut(s) | 51, 1084, 1176, 1273, 1298, 1489 |
| SchI | GAGTC | 9 cut(s) | 136, 165, 208, 305, 595, 686, 794, 1190, 1493 |
| ScrFI | CCNGG | 7 cut(s) | 785, 1055, 1060, 1082, 1135, 1270, 1271 |
| SduI | GDGCHC | 3 cut(s) | 324, 445, 954 |
| SfaNI | GCATC | 1 cut(s) | 381 |
| SfcI | CTRYAG | 3 cut(s) | 279, 853, 1370 |
| SinI | GGWCC | 3 cut(s) | 1176, 1273, 1489 |
| SmaI | CCCGGG | 1 cut(s) | 1271 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 574, 592, 837 |
| Sse9I | AATT | 1 cut(s) | 1396 |
| SseBI | AGGCCT | 2 cut(s) | 343, 701 |
| StuI | AGGCCT | 2 cut(s) | 343, 701 |
| StyD4I | CCNGG | 7 cut(s) | 783, 1053, 1058, 1080, 1133, 1268, 1269 |
| StyI | CCWWGG | 1 cut(s) | 696 |
| TaaI | ACNGT | 8 cut(s) | 333, 591, 664, 938, 1123, 1155, 1219, 1342 |
| TaiI | ACGT | 6 cut(s) | 109, 308, 557, 741, 990, 1293 |
| TaqII | GACCGA | 3 cut(s) | 128, 375, 1193 |
| TasI | AATT | 1 cut(s) | 1396 |
| TauI | GCSGC | 4 cut(s) | 212, 644, 902, 1167 |
| TfiI | GAWTC | 2 cut(s) | 310, 1506 |
| Tru1I | TTAA | 3 cut(s) | 224, 456, 1540 |
| Tru9I | TTAA | 3 cut(s) | 224, 456, 1540 |
| TscAI | CASTG | 4 cut(s) | 234, 244, 306, 594 |
| TseFI | GTSAC | 4 cut(s) | 297, 724, 1213, 1219 |
| TseI | GCWGC | 4 cut(s) | 94, 97, 855, 1369 |
| Tsp45I | GTSAC | 4 cut(s) | 297, 724, 1213, 1219 |
| TspDTI | ATGAA | 4 cut(s) | 57, 170, 769, 1442 |
| TspGWI | ACGGA | 2 cut(s) | 207, 916 |
| TspMI | CCCGGG | 1 cut(s) | 1269 |
| TspRI | CASTG | 4 cut(s) | 234, 244, 306, 594 |
| Tth111I | GACNNNGTC | 4 cut(s) | 217, 553, 1016, 1180 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1093, 1282 |
| VpaK11BI | GGWCC | 3 cut(s) | 1176, 1273, 1489 |
| XmaI | CCCGGG | 1 cut(s) | 1269 |
| XmiI | GTMKAC | 4 cut(s) | 633, 690, 747, 1265 |
| ZraI | GACGTC | 5 cut(s) | 107, 306, 555, 739, 988 |