Rmu_sc0002200.1_g000049

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002200.1
OG0000184
Physical Location & Seq
Reverse (-)
225378 .. 230688
5311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002200.1_g000049.1.cds

Sequence Viewer

Length: 2322 bp
atgatcgtatcgacacgtagaacacaaaaatggacagaaactgtcctttgggtggccggaggtcgccggaaaccaccaccacagtggcggcaccgccgcccatccaaattcagaattagccaaaactcccaacatgaaagtccttcatctaagcatgcttttctgagctcaaccaagaaaaatcgaagccttgccgacgtcggagctgtgttttccggccgggtccgatttcttcaaactgggtcgacttgcagcgccgccgtgagtgagaatcgtcgatggagaccaccagagggaggaccagacggcggaggcgatcctgtctggaaagtttcgtcgccggaggtggccggagctgcgagttccgaccgggtcggaacgccggtcctgctcatcatcgcttgcgtacgacttgcagaatttgcccttcaacagcgagaagtggtgcttggctctgagcatccactcgccgtcgtgaccgtggtggtggccttcattgtcaccgtccacgacttgacggatcaatggattcctgccgtccacttcagcacgagcgaggaagacgaggagagagaagaacaggaagagagagagagagagtacggctgtaggaaccaggaagaaaagatgaggttggatacttgtcgagtccatattgagctaaccatatatggctgttcaagctttgaatctcagctcactatgcttacatgggaagaagcgcgagcaattttatctttaattggatgtagcaattttatttctacattcatatgcttctgcgagtgggttccgcatgttctaatttggatggttgcggctccaatatttgttccactggttgcaattcagtgggcatgtaaatcaattgcggttgcaatttggttggcatgcaattcaattgcggttgcaattcggtgggcatggaaggcttttcttgacatccctgcagcaaagtgggaacatgtatttgtaatatcatgtattgcggtcttactggatcctctgttcttttacattccaatcatcgacgagaaaaataaatgtctttcaatggacaaaaagttgagaactacagctctcgtcttccgatcgcttacggatgcccctttagtactccatattaaagatcaaatttgtgatcgggtccactctgactaccccaactggtccgagtctgactccaactccgactccgactccgacacctacttcgactcccattctgaggcaacatcgtcttcattgaagaagaggatagcaaagaggatgcagtggttgtccttgtcttcattgaagaagaggatagcaaagaggatgcagtggttgtccttgtcttcattgaagaagaggatagcaaagaggatgcagtggttgtccttctctattatagttaactgccttgccattcttcccataccacaggtggctatattagttggattttaccaaatgcaagcatgctcatcgttctctgaagtttctactagaagaacaacgctcaacgtttctcttgccctccagtgtctgccaagatcccaacaatccactccctcctcctgggctttgctcccttggaggacccggatctcgttatcgcttctgtcaaagttaacagacgccgctcgccgaccaaacggcaccatcaaccgccgtctcatgagcttcctcaatttacgctcctccgccactccctcagctccctacaaaggcgtcaccacctccgacgtcaccgtcgaccaaacccgcaacctctggttccgcttcttcgtcccctctacgacgtcgtcttcccccgcctctttaccggtcgtcgttttcttccacggcggaggctttagctttctcagccccgactcctacgcctacgacgccgtctgccgcaaattcgcccgcgaaatccccgccgttgtagtctccgtcaactaccgcctctccctggagcactgctacccctgccagtacgacgacggcctcgacgtcctcaaattcctcgaccaaaaccccaactccctccccgacctcgctgactgctcccgctgcttcctcgtcggagacagcgtcggcgcgaacattgcccaccacgtggaggtgcgagcctgctgcgagccgtttcgggtcgtgaaaccaatcaggttgatatcgatccaactgttcttcggcggtgaggaacggaccgagtccgagatccgactcgagggtgcgccgctggtgtcggtgacccgaaccgattggctgtggaaggctttcttgacacaaaacccagaatcctgccgcaaaacccagaaacccggtggcttcgttgagactgaggagatttga

Protein Analysis

773

Amino Acids

87.09

Weight (kDa)

8.8

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1733
AatII GACGTC 4 cut(s) 201, 1731, 1787, 1983
AccB1I GGYRCC 2 cut(s) 90, 1640
AccB7I CCANNNNNTGG 1 cut(s) 2086
AccBSI CCGCTC 1 cut(s) 1625
AccI GTMKAC 2 cut(s) 245, 1737
AccII CGCG 3 cut(s) 724, 1896, 2069
AclI AACGTT 1 cut(s) 1506
AclWI GGATC 8 cut(s) 311, 528, 995, 1008, 1530, 1595, 2140, 2182
AcoI YGGCCR 3 cut(s) 54, 217, 348
AcsI RAATTY 5 cut(s) 107, 419, 1134, 1886, 1988
AcuI CTGAAG 2 cut(s) 529, 1497
AcvI CACGTG 1 cut(s) 2086
AcyI GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
AdeI CACNNNGTG 1 cut(s) 2086
AfaI GTAC 4 cut(s) 408, 602, 1116, 1964
AflIII ACRYGT 2 cut(s) 14, 964
AgeI ACCGGT 1 cut(s) 1807
AgsI TTSAA 9 cut(s) 236, 431, 681, 689, 900, 1053, 1249, 1297, 1345
AjnI CCWGG 3 cut(s) 615, 1559, 1938
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AleI CACNNNNGTG 1 cut(s) 82
AloI GAACNNNNNNTCC 2 cut(s) 992, 1024
Alw21I GWGCWC 2 cut(s) 170, 1947
Alw26I GTCTC 5 cut(s) 277, 1661, 1921, 2049, 2300
AlwI GGATC 8 cut(s) 311, 528, 995, 1008, 1530, 1595, 2140, 2182
AlwNI CAGNNNCTG 2 cut(s) 41, 1528
Ama87I CYCGRG 1 cut(s) 2195
AoxI GGCC 5 cut(s) 54, 217, 348, 489, 1972
ApeKI GCWGC 5 cut(s) 252, 356, 950, 2040, 2103
ApoI RAATTY 5 cut(s) 107, 419, 1134, 1886, 1988
ArsI GACNNNNNNTTYG 2 cut(s) 1196, 1228
AsiGI ACCGGT 1 cut(s) 1807
Asp700I GAANNNNTTC 1 cut(s) 2246
AspLEI GCGC 4 cut(s) 257, 724, 2069, 2206
AspS9I GGNCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
AsuC2I CCSGG 4 cut(s) 221, 371, 1585, 2292
AsuHPI GGTGA 5 cut(s) 493, 1708, 1723, 2177, 2230
AvaI CYCGRG 1 cut(s) 2195
AvaII GGWCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
BamHI GGATCC 1 cut(s) 1000
BanI GGYRCC 2 cut(s) 90, 1640
BanII GRGCYC 1 cut(s) 170
BauI CACGAG 1 cut(s) 550
BbrPI CACGTG 1 cut(s) 2086
BbsI GAAGAC 6 cut(s) 567, 1078, 1233, 1281, 1329, 1782
Bbv12I GWGCWC 2 cut(s) 170, 1947
BbvCI CCTCAGC 1 cut(s) 1696
BbvI GCAGC 5 cut(s) 264, 343, 962, 2027, 2090
BccI CCATC 4 cut(s) 109, 273, 805, 1652
BcgI CGANNNNNNTGC 4 cut(s) 1449, 1483, 2085, 2119
BciT130I CCWGG 3 cut(s) 617, 1561, 1940
BciVI GTATCC 1 cut(s) 631
BcnI CCSGG 4 cut(s) 221, 371, 1585, 2292
BcoDI GTCTC 5 cut(s) 277, 1661, 1921, 2049, 2300
BfaI CTAG 1 cut(s) 1488
BfmI CTRYAG 3 cut(s) 607, 948, 1074
BfoI RGCGCY 1 cut(s) 258
BfuI GTATCC 1 cut(s) 631
BmcAI AGTACT 1 cut(s) 1116
Bme1390I CCNGG 7 cut(s) 221, 371, 617, 1561, 1585, 1940, 2292
Bme18I GGWCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
BmeT110I CYCGRG 1 cut(s) 2195
BmgT120I GGNCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
BmrFI CCNGG 7 cut(s) 221, 371, 617, 1561, 1585, 1940, 2292
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 5 cut(s) 469, 1093, 1260, 1308, 1356
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 6 cut(s) 567, 1078, 1233, 1281, 1329, 1782
BplI GAGNNNNNCTC 2 cut(s) 1166, 1198
BpmI CTGGAG 2 cut(s) 1505, 1961
Bpu10I CCTNAGC 1 cut(s) 1696
BpuMI CCSGG 4 cut(s) 221, 371, 1585, 2292
Bsa29I ATCGAT 1 cut(s) 2144
BsaAI YACGTR 2 cut(s) 17, 2086
BsaBI GATNNNNATC 1 cut(s) 2144
BsaHI GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
BsaI GGTCTC 1 cut(s) 277
BsaJI CCNNGG 5 cut(s) 480, 1560, 1574, 1825, 1938
BsaWI WCCGGW 1 cut(s) 1807
Bse118I RCCGGY 2 cut(s) 382, 1807
Bse1I ACTGG 6 cut(s) 244, 843, 1002, 1172, 1522, 1960
Bse3DI GCAATG 1 cut(s) 2073
Bse8I GATNNNNATC 1 cut(s) 2144
BseBI CCWGG 3 cut(s) 617, 1561, 1940
BseCI ATCGAT 1 cut(s) 2144
BseDI CCNNGG 5 cut(s) 480, 1560, 1574, 1825, 1938
BseGI GGATG 9 cut(s) 101, 460, 752, 816, 942, 1108, 1275, 1323, 1371
BseJI GATNNNNATC 1 cut(s) 2144
BseMI GCAATG 1 cut(s) 2073
BseMII CTCAG 7 cut(s) 155, 447, 707, 1218, 1710, 1861, 2301
BseNI ACTGG 6 cut(s) 244, 843, 1002, 1172, 1522, 1960
BseRI GAGGAG 3 cut(s) 581, 1546, 1672
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 5 cut(s) 264, 343, 962, 2027, 2090
Bsh1236I CGCG 3 cut(s) 724, 1896, 2069
Bsh1285I CGRYCG 4 cut(s) 220, 370, 1094, 1812
BshFI GGCC 5 cut(s) 56, 219, 350, 491, 1974
BshNI GGYRCC 2 cut(s) 90, 1640
BshTI ACCGGT 1 cut(s) 1807
BshVI ATCGAT 1 cut(s) 2144
BsiEI CGRYCG 4 cut(s) 220, 370, 1094, 1812
BsiHKAI GWGCWC 2 cut(s) 170, 1947
BsiHKCI CYCGRG 1 cut(s) 2195
BsiWI CGTACG 1 cut(s) 406
BslFI GGGAC 1 cut(s) 1757
BsmAI GTCTC 5 cut(s) 277, 1661, 1921, 2049, 2300
BsmBI CGTCTC 1 cut(s) 1661
BsmFI GGGAC 1 cut(s) 1757
BsnI GGCC 5 cut(s) 56, 219, 350, 491, 1974
Bso31I GGTCTC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 2195
Bsp1286I GDGCHC 2 cut(s) 170, 1947
BspANI GGCC 5 cut(s) 56, 219, 350, 491, 1974
BspCNI CTCAG 7 cut(s) 156, 448, 706, 1219, 1709, 1860, 2302
BspDI ATCGAT 1 cut(s) 2144
BspFNI CGCG 3 cut(s) 724, 1896, 2069
BspHI TCATGA 1 cut(s) 1659
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 8 cut(s) 311, 528, 995, 1008, 1530, 1595, 2140, 2182
BspT107I GGYRCC 2 cut(s) 90, 1640
BspTNI GGTCTC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 1625
BsrDI GCAATG 1 cut(s) 2073
BsrFI RCCGGY 2 cut(s) 382, 1807
BsrI ACTGG 6 cut(s) 244, 843, 1002, 1172, 1522, 1960
BssAI RCCGGY 2 cut(s) 382, 1807
BssECI CCNNGG 5 cut(s) 480, 1560, 1574, 1825, 1938
BssNI GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
BssSI CACGAG 1 cut(s) 550
BssT1I CCWWGG 1 cut(s) 1574
Bst2BI CACGAG 1 cut(s) 550
Bst2UI CCWGG 3 cut(s) 617, 1561, 1940
Bst4CI ACNGT 6 cut(s) 43, 84, 481, 505, 1735, 2154
Bst6I CTCTTC 4 cut(s) 579, 1247, 1295, 1343
BstACI GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBAI YACGTR 2 cut(s) 17, 2086
BstDEI CTNAG 8 cut(s) 150, 164, 456, 693, 1227, 1696, 1847, 2310
BstDSI CCRYGG 2 cut(s) 480, 1825
BstEII GGTNACC 1 cut(s) 2218
BstF5I GGATG 9 cut(s) 101, 460, 752, 816, 942, 1108, 1275, 1323, 1371
BstFNI CGCG 3 cut(s) 724, 1896, 2069
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 4 cut(s) 257, 724, 2069, 2206
BstMAI GTCTC 5 cut(s) 277, 1661, 1921, 2049, 2300
BstMCI CGRYCG 4 cut(s) 220, 370, 1094, 1812
BstNI CCWGG 3 cut(s) 617, 1561, 1940
BstNSI RCATGY 6 cut(s) 158, 800, 861, 894, 968, 1464
BstPI GGTNACC 1 cut(s) 2218
BstSCI CCNGG 7 cut(s) 219, 369, 615, 1559, 1583, 1938, 2290
BstSFI CTRYAG 3 cut(s) 607, 948, 1074
BstUI CGCG 3 cut(s) 724, 1896, 2069
BstV1I GCAGC 5 cut(s) 264, 343, 962, 2027, 2090
BstV2I GAAGAC 6 cut(s) 567, 1078, 1233, 1281, 1329, 1782
BstX2I RGATCY 4 cut(s) 1000, 1535, 1587, 2187
BstXI CCANNNNNNTGG 1 cut(s) 84
BstYI RGATCY 4 cut(s) 1000, 1535, 1587, 2187
BstZI CGGCCG 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 2144
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 5 cut(s) 56, 219, 350, 491, 1974
BsuTUI ATCGAT 1 cut(s) 2144
BtgI CCRYGG 2 cut(s) 480, 1825
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 9 cut(s) 101, 460, 752, 816, 942, 1108, 1275, 1323, 1371
BtsI GCAGTG 4 cut(s) 1280, 1328, 1376, 1945
BtsIMutI CAGTG 8 cut(s) 89, 836, 857, 1280, 1328, 1376, 1529, 1945
CaiI CAGNNNCTG 2 cut(s) 41, 1528
CciI TCATGA 1 cut(s) 1659
CfoI GCGC 4 cut(s) 257, 724, 2069, 2206
Cfr10I RCCGGY 2 cut(s) 382, 1807
Cfr13I GGNCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
ClaI ATCGAT 1 cut(s) 2144
CpoI CGGWCCG 1 cut(s) 2175
CseI GACGC 4 cut(s) 1628, 1702, 1880, 2050
Csp6I GTAC 4 cut(s) 407, 601, 1115, 1963
CspAI ACCGGT 1 cut(s) 1807
CspI CGGWCCG 1 cut(s) 2175
CviQI GTAC 4 cut(s) 407, 601, 1115, 1963
DdeI CTNAG 8 cut(s) 150, 164, 456, 693, 1227, 1696, 1847, 2310
DraIII CACNNNGTG 1 cut(s) 2086
DrdI GACNNNNNNGTC 1 cut(s) 1733
DseDI GACNNNNNNGTC 1 cut(s) 1733
EaeI YGGCCR 3 cut(s) 54, 217, 348
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 4 cut(s) 579, 1247, 1295, 1343
EarI CTCTTC 4 cut(s) 579, 1247, 1295, 1343
EciI GGCGGA 3 cut(s) 324, 1675, 1845
Ecl136II GAGCTC 1 cut(s) 168
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 1574
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 277
Eco32I GATATC 1 cut(s) 2142
Eco47I GGWCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 529, 1497
Eco72I CACGTG 1 cut(s) 2086
Eco88I CYCGRG 1 cut(s) 2195
Eco91I GGTNACC 1 cut(s) 2218
EcoICRI GAGCTC 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 1581
EcoO65I GGTNACC 1 cut(s) 2218
EcoRII CCWGG 3 cut(s) 615, 1559, 1938
EcoRV GATATC 1 cut(s) 2142
EcoT14I CCWWGG 1 cut(s) 1574
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 1574
Esp3I CGTCTC 1 cut(s) 1661
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 2234, 2266
FaqI GGGAC 1 cut(s) 1757
FauI CCCGC 5 cut(s) 1754, 1804, 1901, 1912, 2045
FauNDI CATATG 1 cut(s) 773
FblI GTMKAC 2 cut(s) 245, 1737
FokI GGATG 9 cut(s) 88, 447, 759, 823, 929, 1115, 1282, 1330, 1378
FriOI GRGCYC 1 cut(s) 170
FspBI CTAG 1 cut(s) 1488
GlaI GCGC 4 cut(s) 256, 723, 2068, 2205
GsuI CTGGAG 2 cut(s) 1505, 1961
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 5 cut(s) 56, 219, 350, 491, 1974
HgaI GACGC 4 cut(s) 1628, 1702, 1880, 2050
HhaI GCGC 4 cut(s) 257, 724, 2069, 2206
Hin1I GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
Hin6I GCGC 4 cut(s) 255, 722, 2067, 2204
HinP1I GCGC 4 cut(s) 255, 722, 2067, 2204
HincII GTYRAC 5 cut(s) 246, 1396, 1614, 1738, 1924
HindII GTYRAC 5 cut(s) 246, 1396, 1614, 1738, 1924
HindIII AAGCTT 1 cut(s) 682
HpaI GTTAAC 2 cut(s) 1396, 1614
HphI GGTGA 5 cut(s) 493, 1708, 1723, 2177, 2230
Hpy166II GTNNAC 8 cut(s) 246, 508, 541, 1150, 1396, 1614, 1738, 1924
Hpy188III TCNNGA 6 cut(s) 325, 475, 938, 1660, 2122, 2251
Hpy8I GTNNAC 8 cut(s) 246, 508, 541, 1150, 1396, 1614, 1738, 1924
HpyAV CCTTC 6 cut(s) 153, 437, 502, 922, 1390, 2236
HpyCH4III ACNGT 6 cut(s) 43, 84, 481, 505, 1735, 2154
HpyCH4IV ACGT 7 cut(s) 16, 198, 1506, 1728, 1784, 1980, 2085
HpyF3I CTNAG 8 cut(s) 150, 164, 456, 693, 1227, 1696, 1847, 2310
HpySE526I ACGT 7 cut(s) 16, 198, 1506, 1728, 1784, 1980, 2085
Hsp92I GRCGYC 7 cut(s) 198, 1620, 1713, 1728, 1784, 1872, 1980
HspAI GCGC 4 cut(s) 255, 722, 2067, 2204
KspAI GTTAAC 2 cut(s) 1396, 1614
LmnI GCTCC 8 cut(s) 203, 353, 826, 1575, 1685, 1705, 1942, 2039
Lsp1109I GCAGC 5 cut(s) 264, 343, 962, 2027, 2090
LweI GCATC 5 cut(s) 469, 1093, 1260, 1308, 1356
MaeI CTAG 1 cut(s) 1488
MaeII ACGT 7 cut(s) 16, 198, 1506, 1728, 1784, 1980, 2085
MaeIII GTNAC 5 cut(s) 475, 499, 1714, 1729, 2218
MbiI CCGCTC 1 cut(s) 1625
MfeI CAATTG 2 cut(s) 867, 900
MflI RGATCY 4 cut(s) 1000, 1535, 1587, 2187
MhlI GDGCHC 2 cut(s) 170, 1947
MlyI GAGTC 9 cut(s) 657, 1175, 1184, 1187, 1193, 1211, 1850, 2187, 2189
MroXI GAANNNNTTC 1 cut(s) 2246
MseI TTAA 4 cut(s) 740, 1125, 1395, 1613
MslI CAYNNNNRTG 3 cut(s) 28, 82, 772
MspA1I CMGCKG 2 cut(s) 2040, 2209
MspR9I CCNGG 7 cut(s) 221, 371, 617, 1561, 1585, 1940, 2292
MunI CAATTG 2 cut(s) 867, 900
MvaI CCWGG 3 cut(s) 617, 1561, 1940
MvnI CGCG 3 cut(s) 724, 1896, 2069
NciI CCSGG 4 cut(s) 221, 371, 1585, 2292
NdeI CATATG 1 cut(s) 773
NmuCI GTSAC 5 cut(s) 475, 499, 1714, 1729, 2218
NspI RCATGY 6 cut(s) 158, 800, 861, 894, 968, 1464
OliI CACNNNNGTG 1 cut(s) 82
PaeI GCATGC 3 cut(s) 158, 894, 1464
PaeR7I CTCGAG 1 cut(s) 2195
PagI TCATGA 1 cut(s) 1659
PciI ACATGT 1 cut(s) 964
PcsI WCGNNNNNNNCGW 5 cut(s) 312, 1734, 1974, 1977, 2058
PdmI GAANNNNTTC 1 cut(s) 2246
PfeI GAWTC 4 cut(s) 271, 529, 689, 2267
Pfl23II CGTACG 1 cut(s) 406
PflFI GACNNNGTC 5 cut(s) 371, 1786, 1874, 2060, 2179
PflMI CCANNNNNTGG 1 cut(s) 2086
PinAI ACCGGT 1 cut(s) 1807
Ple19I CGATCG 1 cut(s) 1094
PleI GAGTC 9 cut(s) 656, 1175, 1183, 1187, 1193, 1211, 1850, 2187, 2188
PmaCI CACGTG 1 cut(s) 2086
PmlI CACGTG 1 cut(s) 2086
PpsI GAGTC 9 cut(s) 656, 1175, 1183, 1187, 1193, 1211, 1850, 2187, 2188
Ppu21I YACGTR 2 cut(s) 17, 2086
PpuMI RGGWCCY 1 cut(s) 1581
PscI ACATGT 1 cut(s) 964
Psp124BI GAGCTC 1 cut(s) 170
Psp1406I AACGTT 1 cut(s) 1506
Psp5II RGGWCCY 1 cut(s) 1581
Psp6I CCWGG 3 cut(s) 615, 1559, 1938
PspCI CACGTG 1 cut(s) 2086
PspEI GGTNACC 1 cut(s) 2218
PspGI CCWGG 3 cut(s) 615, 1559, 1938
PspLI CGTACG 1 cut(s) 406
PspPI GGNCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
PspPPI RGGWCCY 1 cut(s) 1581
PspXI VCTCGAGB 1 cut(s) 2195
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 2 cut(s) 41, 1528
PsuI RGATCY 4 cut(s) 1000, 1535, 1587, 2187
PsyI GACNNNGTC 5 cut(s) 371, 1786, 1874, 2060, 2179
PvuI CGATCG 1 cut(s) 1094
RsaI GTAC 4 cut(s) 408, 602, 1116, 1964
RsaNI GTAC 4 cut(s) 407, 601, 1115, 1963
RseI CAYNNNNRTG 3 cut(s) 28, 82, 772
Rsr2I CGGWCCG 1 cut(s) 2175
RsrII CGGWCCG 1 cut(s) 2175
SacI GAGCTC 1 cut(s) 170
SalI GTCGAC 2 cut(s) 244, 1736
SaqAI TTAA 4 cut(s) 740, 1125, 1395, 1613
Sau96I GGNCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
ScaI AGTACT 1 cut(s) 1116
SchI GAGTC 9 cut(s) 657, 1175, 1184, 1187, 1193, 1211, 1850, 2187, 2189
ScrFI CCNGG 7 cut(s) 221, 371, 617, 1561, 1585, 1940, 2292
SduI GDGCHC 2 cut(s) 170, 1947
SfaNI GCATC 5 cut(s) 469, 1093, 1260, 1308, 1356
SfcI CTRYAG 3 cut(s) 607, 948, 1074
Sfr274I CTCGAG 1 cut(s) 2195
SinI GGWCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
SlaI CTCGAG 1 cut(s) 2195
SmiMI CAYNNNNRTG 3 cut(s) 28, 82, 772
SmlI CTYRAG 1 cut(s) 2195
SmoI CTYRAG 1 cut(s) 2195
SphI GCATGC 3 cut(s) 158, 894, 1464
SspI AATATT 1 cut(s) 828
SspMI CTAG 1 cut(s) 1488
SstI GAGCTC 1 cut(s) 170
StyD4I CCNGG 7 cut(s) 219, 369, 615, 1559, 1583, 1938, 2290
StyI CCWWGG 1 cut(s) 1574
TaaI ACNGT 6 cut(s) 43, 84, 481, 505, 1735, 2154
TaiI ACGT 7 cut(s) 19, 201, 1509, 1731, 1787, 1983, 2088
TaqII GACCGA 1 cut(s) 2192
TatI WGTACW 1 cut(s) 1114
TauI GCSGC 8 cut(s) 91, 99, 260, 821, 1625, 1884, 2209, 2277
TfiI GAWTC 4 cut(s) 271, 529, 689, 2267
Tru1I TTAA 4 cut(s) 740, 1125, 1395, 1613
Tru9I TTAA 4 cut(s) 740, 1125, 1395, 1613
TscAI CASTG 8 cut(s) 89, 843, 857, 1280, 1328, 1376, 1529, 1952
TseFI GTSAC 5 cut(s) 475, 499, 1714, 1729, 2218
TseI GCWGC 5 cut(s) 252, 356, 950, 2040, 2103
Tsp45I GTSAC 5 cut(s) 475, 499, 1714, 1729, 2218
TspDTI ATGAA 7 cut(s) 135, 150, 484, 760, 1233, 1281, 1329
TspGWI ACGGA 4 cut(s) 533, 1115, 1909, 2188
TspRI CASTG 8 cut(s) 89, 843, 857, 1280, 1328, 1376, 1529, 1952
Tth111I GACNNNGTC 5 cut(s) 371, 1786, 1874, 2060, 2179
Van91I CCANNNNNTGG 1 cut(s) 2086
VpaK11BI GGWCC 7 cut(s) 223, 299, 385, 1147, 1170, 1581, 2175
XapI RAATTY 5 cut(s) 107, 419, 1134, 1886, 1988
XceI RCATGY 6 cut(s) 158, 800, 861, 894, 968, 1464
XcmI CCANNNNNNNNNTGG 3 cut(s) 81, 1423, 2291
XhoI CTCGAG 1 cut(s) 2195
XmiI GTMKAC 2 cut(s) 245, 1737
XmnI GAANNNNTTC 1 cut(s) 2246
XspI CTAG 1 cut(s) 1488
ZraI GACGTC 4 cut(s) 199, 1729, 1785, 1981
ZrmI AGTACT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.