RchiOBHm_Chr4g0422631

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000184
Physical Location & Seq
Reverse (-)
48192636 .. 48193298
663 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39209

Sequence Viewer

Length: 663 bp
ATGAGCTTCCTCGACTTACGCTCCTCCGCCACTCCCTCAGCTCCCTACAAAGGCGTCACCACCTCCAACGTCACCGTCCACCAAACCCGCAACCTCTGGTTCCGCTTCTTCGTCCCCTCTACGACGTCGTCTTCCCCCGCCTCTTTACCCGTCGTCGTTTTCTTCCACGACGGCGGCTTCAGCTTTCTCAGCCCCGACTCCTATGCCTACGACGCCGTCTGCAGCAAATTCCCCCGCGAAATCCCCGCCGTCGTAGTCTCCGTCAACTACCGCCTCTCCCCGGAGCACGGCTACCCCTGCCAGTACGATGACGGCCTCAACGTCCTCAAATTCCTCGACCAAAACCCCGACTCCCTCCCCGACCTCGCTGACTGCTCCCGCTGCTTCCTCGCAGGAGACAGCGCCGACGCGAACATTGCCCACCACGTGGCGGTGCGAGCCTGCCGCGAGCCGTTTCGGGTCGTGAAACCAATCAGGTTGATATCGATCCAACCGTTCTTCGGCGGCGAGGAACGGACCGAGTCCGAGATCCGACTAGAGGGCGCGCCGCTGGTGTCGGTGACGCGAACCGATTGGCTGTGGAAGGCATTCTTGCCACAAAACCCAGAATCCTGCCACAAAACCCAGAAACCCGGTGGCTTCGTTGAGACTGAGGAGATTTGA

Protein Analysis

220

Amino Acids

24.48

Weight (kDa)

5.66

Isoelectric Point (pI)

60.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Say1_Mug180 PF10340 25 - 177 7.6e-06 Steryl acetyl hydrolase
COesterase PF00135 36 - 94 5.1e-07 Carboxylesterase family
BD-FAE PF20434 46 - 146 1.2e-07 BD-FAE
Abhydrolase_3 PF07859 52 - 201 5.9e-33 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 427
AccII CGCG 5 cut(s) 237, 410, 447, 545, 565
AclWI GGATC 2 cut(s) 481, 523
AcsI RAATTY 2 cut(s) 227, 329
AcuI CTGAAG 1 cut(s) 163
AcvI CACGTG 1 cut(s) 427
AcyI GRCGYC 3 cut(s) 54, 125, 213
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 7 cut(s) 50, 280, 287, 427, 430, 500, 538
AluBI AGCT 3 cut(s) 6, 41, 183
AluI AGCT 3 cut(s) 6, 41, 183
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 262, 390, 641
AlwI GGATC 2 cut(s) 481, 523
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 222, 381
ApoI RAATTY 2 cut(s) 227, 329
AscI GGCGCGCC 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 587
AspLEI GCGC 3 cut(s) 404, 545, 547
AspS9I GGNCC 1 cut(s) 516
AsuC2I CCSGG 2 cut(s) 281, 633
AsuHPI GGTGA 3 cut(s) 49, 64, 571
AvaII GGWCC 1 cut(s) 516
BbrPI CACGTG 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 234, 368
BceAI ACGGC 6 cut(s) 187, 200, 233, 304, 328, 436
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcnI CCSGG 2 cut(s) 281, 633
BcoDI GTCTC 3 cut(s) 262, 390, 641
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 6 cut(s) 175, 223, 382, 445, 505, 548
BlsI GCNGC 6 cut(s) 176, 224, 383, 446, 506, 549
Bme1390I CCNGG 2 cut(s) 281, 633
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 281, 633
BpiI GAAGAC 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 37
BpuMI CCSGG 2 cut(s) 281, 633
Bsa29I ATCGAT 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 485
BsaHI GRCGYC 3 cut(s) 54, 125, 213
BsaJI CCNNGG 1 cut(s) 279
BsaXI ACNNNNNCTCC 6 cut(s) 5, 35, 260, 290, 335, 365
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 280, 287, 427, 430, 500, 538
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 485
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 7 cut(s) 50, 280, 287, 427, 430, 500, 538
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 51, 202, 642
BseNI ACTGG 1 cut(s) 301
BsePI GCGCGC 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 2 cut(s) 234, 368
Bsh1236I CGCG 5 cut(s) 237, 410, 447, 545, 565
BshFI GGCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 281, 633
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 7 cut(s) 50, 280, 287, 427, 430, 500, 538
BsmAI GTCTC 3 cut(s) 262, 390, 641
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 2 cut(s) 486, 528
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 3 cut(s) 50, 201, 643
BspDI ATCGAT 1 cut(s) 485
BspFNI CGCG 5 cut(s) 237, 410, 447, 545, 565
BspLI GGNNCC 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 224
BspPI GGATC 2 cut(s) 481, 523
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 279
BssHII GCGCGC 1 cut(s) 543
BssMI GATC 2 cut(s) 486, 528
BssNI GRCGYC 3 cut(s) 54, 125, 213
Bst4CI ACNGT 2 cut(s) 76, 495
BstACI GRCGYC 3 cut(s) 54, 125, 213
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 4 cut(s) 438, 442, 449, 545
BstDEI CTNAG 3 cut(s) 37, 188, 651
BstFNI CGCG 5 cut(s) 237, 410, 447, 545, 565
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 3 cut(s) 404, 545, 547
BstKTI GATC 2 cut(s) 489, 531
BstMAI GTCTC 3 cut(s) 262, 390, 641
BstMBI GATC 2 cut(s) 486, 528
BstMWI GCNNNNNNNGC 7 cut(s) 180, 189, 212, 297, 381, 416, 437
BstSCI CCNGG 2 cut(s) 279, 631
BstSFI CTRYAG 1 cut(s) 220
BstUI CGCG 5 cut(s) 237, 410, 447, 545, 565
BstV1I GCAGC 2 cut(s) 234, 368
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 528
BstYI RGATCY 1 cut(s) 528
Bsu15I ATCGAT 1 cut(s) 485
BsuRI GGCC 1 cut(s) 315
BsuTUI ATCGAT 1 cut(s) 485
Cac8I GCNNGC 4 cut(s) 438, 442, 449, 545
CfoI GCGC 3 cut(s) 404, 545, 547
Cfr13I GGNCC 1 cut(s) 516
ClaI ATCGAT 1 cut(s) 485
CpoI CGGWCCG 1 cut(s) 516
CseI GACGC 4 cut(s) 43, 221, 416, 571
Csp6I GTAC 1 cut(s) 304
CspI CGGWCCG 1 cut(s) 516
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 3 cut(s) 37, 188, 651
DpnI GATC 2 cut(s) 488, 530
DpnII GATC 2 cut(s) 486, 528
DraIII CACNNNGTG 1 cut(s) 427
EciI GGCGGA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 163
Eco72I CACGTG 1 cut(s) 427
EcoRV GATATC 1 cut(s) 483
FaiI YATR 1 cut(s) 204
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 1 cut(s) 98
FauI CCCGC 5 cut(s) 95, 145, 242, 253, 386
Fnu4HI GCNGC 6 cut(s) 175, 223, 382, 445, 505, 548
Fsp4HI GCNGC 6 cut(s) 175, 223, 382, 445, 505, 548
FspBI CTAG 1 cut(s) 536
GlaI GCGC 3 cut(s) 403, 544, 546
GluI GCNGC 6 cut(s) 175, 223, 382, 445, 505, 548
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 281, 633
HgaI GACGC 4 cut(s) 43, 221, 416, 571
HhaI GCGC 3 cut(s) 404, 545, 547
Hin1I GRCGYC 3 cut(s) 54, 125, 213
Hin6I GCGC 3 cut(s) 402, 543, 545
HinP1I GCGC 3 cut(s) 402, 543, 545
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 4 cut(s) 197, 350, 521, 608
HpaII CCGG 2 cut(s) 281, 633
HphI GGTGA 3 cut(s) 49, 64, 571
Hpy166II GTNNAC 2 cut(s) 79, 265
Hpy188I TCNGA 2 cut(s) 526, 533
Hpy188III TCNNGA 1 cut(s) 463
Hpy8I GTNNAC 2 cut(s) 79, 265
Hpy99I CGWCG 8 cut(s) 127, 130, 155, 158, 173, 215, 254, 410
HpyAV CCTTC 1 cut(s) 577
HpyCH4III ACNGT 2 cut(s) 76, 495
HpyCH4IV ACGT 4 cut(s) 69, 125, 321, 426
HpyCH4V TGCA 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 189, 212, 297, 381, 416, 437
HpyF3I CTNAG 3 cut(s) 37, 188, 651
HpySE526I ACGT 4 cut(s) 69, 125, 321, 426
Hsp92I GRCGYC 3 cut(s) 54, 125, 213
HspAI GCGC 3 cut(s) 402, 543, 545
Kzo9I GATC 2 cut(s) 486, 528
LmnI GCTCC 4 cut(s) 26, 46, 283, 380
Lsp1109I GCAGC 2 cut(s) 234, 368
MaeI CTAG 1 cut(s) 536
MaeII ACGT 4 cut(s) 69, 125, 321, 426
MaeIII GTNAC 3 cut(s) 55, 70, 559
MalI GATC 2 cut(s) 488, 530
MboI GATC 2 cut(s) 486, 528
MboII GAAGA 4 cut(s) 100, 123, 154, 490
MflI RGATCY 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 2 cut(s) 227, 329
MlyI GAGTC 3 cut(s) 191, 344, 530
MmeI TCCRAC 3 cut(s) 90, 514, 556
MroXI GAANNNNTTC 1 cut(s) 587
MspA1I CMGCKG 2 cut(s) 381, 550
MspI CCGG 2 cut(s) 281, 633
MspR9I CCNGG 2 cut(s) 281, 633
Mva1269I GAATGC 1 cut(s) 587
MvnI CGCG 5 cut(s) 237, 410, 447, 545, 565
MwoI GCNNNNNNNGC 7 cut(s) 180, 189, 212, 297, 381, 416, 437
NciI CCSGG 2 cut(s) 281, 633
NdeII GATC 2 cut(s) 486, 528
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 3 cut(s) 55, 70, 559
PalAI GGCGCGCC 1 cut(s) 543
PauI GCGCGC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 318, 491
PctI GAATGC 1 cut(s) 587
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 1 cut(s) 608
PflFI GACNNNGTC 3 cut(s) 127, 215, 520
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 6 cut(s) 176, 224, 383, 446, 506, 549
PleI GAGTC 3 cut(s) 191, 344, 529
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
PpsI GAGTC 3 cut(s) 191, 344, 529
Ppu21I YACGTR 1 cut(s) 427
PspCI CACGTG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 516
PstI CTGCAG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 528
PsyI GACNNNGTC 3 cut(s) 127, 215, 520
PteI GCGCGC 1 cut(s) 543
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
Rsr2I CGGWCCG 1 cut(s) 516
RsrII CGGWCCG 1 cut(s) 516
SatI GCNGC 6 cut(s) 175, 223, 382, 445, 505, 548
Sau3AI GATC 2 cut(s) 486, 528
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 3 cut(s) 191, 344, 530
ScrFI CCNGG 2 cut(s) 281, 633
SduI GDGCHC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 220
SgsI GGCGCGCC 1 cut(s) 543
SinI GGWCC 1 cut(s) 516
Sse9I AATT 2 cut(s) 227, 329
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 279, 631
TaaI ACNGT 2 cut(s) 76, 495
TaiI ACGT 4 cut(s) 72, 128, 324, 429
TaqI TCGA 3 cut(s) 12, 336, 485
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 227, 329
TauI GCSGC 4 cut(s) 177, 447, 507, 550
TfiI GAWTC 1 cut(s) 608
TseFI GTSAC 3 cut(s) 55, 70, 559
TseI GCWGC 2 cut(s) 222, 381
Tsp45I GTSAC 3 cut(s) 55, 70, 559
TspGWI ACGGA 2 cut(s) 250, 529
Tth111I GACNNNGTC 3 cut(s) 127, 215, 520
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 2 cut(s) 227, 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 1 cut(s) 536
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.