MD05G1060700.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000184
Physical Location & Seq
Forward (+)
10520056 .. 10520928
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1060700.v1.1.491

Sequence Viewer

Length: 873 bp
ATGACGACATCGTTGGAATCCAATACTCAAAAGACCGTAACGTCATGGACTTCTCTCCGCTGGAGGACGCGGGTGTCGTTGTCCCTTCTCTCAAAAGTAACCGACGCCGCACGCCGAGCTGACGGCACTGTCAATTGCCGTCTCATGAACATACTCGACTTCAAGTCCTTAGCCGCTCCTGCCGCTTTCATCAGAGAAGTCACCTTATCCGACGTCACTGTGGACCCCACTCGTAAACTCTGGTTCCGCCTATTCGTCCCCCAAACGACGTTGTCGACTCCATCCCATCTTCCCGTCGTCGTATTCCTCCACGACGGCGGATTCACCTTCCTCAGCTCCGCCTCATTTGCTTACGATGTCGTTTACCGTAAGTTTGCTCGTAAATTTCCCGCTGTCGTCGTATCTGTCGATTACCGCCTCTGCCCCGAACACCGGTACCCTTCCCAATATGACGACGGGTTCGACGTCCTTACCGTCCTCGACCAAAACGACGACGTCCTCCCAAAAAATGCAGACAGATCCAGAGTCTTCTTTGCCGGCGACAGCGCCGGAGCCAACCTGGCCCACCACGTGGCTGTTCGCGCCGCCCGAAAGAAAGATCGATTCCGGGTCGTGAAACCTGTCGGGTTGATATCGATACAACCATTTTTCGGGGGAGAAGAGCGGGTCGAGTCTGAGATCCGTCTCCATGGAGCTCCTCTGGTCTCGGTGGCCCAAACGGACTGGCTGTGGAAAGTGTTTTTGCCAGACGGGTCGGACCGGGACCACAAGGCGGCAAACTCGAGTGGGCCGAACGCGGTGGACATTGCGGGTCTGGAGTATCCGAATACGCTTGTATTCACAGGGGGATTGGATCCGCTACAAGACTGGTAG

Protein Analysis

291

Amino Acids

32.32

Weight (kDa)

8.51

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 84 - 141 2.2e-06 Carboxylesterase family
BD-FAE PF20434 95 - 196 7.7e-10 BD-FAE
Abhydrolase_3 PF07859 100 - 290 2.4e-43 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 40, 73, 128
AatII GACGTC 3 cut(s) 216, 468, 498
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccB7I CCANNNNNTGG 1 cut(s) 571
AccBSI CCGCTC 2 cut(s) 176, 664
AccI GTMKAC 1 cut(s) 275
AccII CGCG 3 cut(s) 70, 582, 797
AclWI GGATC 4 cut(s) 513, 673, 848, 861
AcsI RAATTY 1 cut(s) 383
AcvI CACGTG 1 cut(s) 571
AcyI GRCGYC 4 cut(s) 105, 213, 465, 495
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 4 cut(s) 432, 571, 650, 772
AgeI ACCGGT 1 cut(s) 432
AgsI TTSAA 1 cut(s) 163
AhdI GACNNNNNGTC 1 cut(s) 163
AjnI CCWGG 1 cut(s) 558
AluBI AGCT 3 cut(s) 119, 336, 695
AluI AGCT 3 cut(s) 119, 336, 695
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 3 cut(s) 146, 689, 709
AlwI GGATC 4 cut(s) 513, 673, 848, 861
Ama87I CYCGRG 1 cut(s) 781
AoxI GGCC 3 cut(s) 561, 711, 788
ApoI RAATTY 1 cut(s) 383
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AsiGI ACCGGT 1 cut(s) 432
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 2 cut(s) 548, 584
AspS9I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
AsuC2I CCSGG 2 cut(s) 608, 761
AsuHPI GGTGA 2 cut(s) 193, 316
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 3 cut(s) 223, 757, 763
BaeI ACNNNNGTAYC 2 cut(s) 419, 452
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 697
BbrPI CACGTG 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 697
BbvCI CCTCAGC 1 cut(s) 332
BccI CCATC 2 cut(s) 289, 294
BceAI ACGGC 3 cut(s) 123, 139, 331
BciT130I CCWGG 1 cut(s) 560
BciVI GTATCC 1 cut(s) 831
BcnI CCSGG 2 cut(s) 608, 761
BcoDI GTCTC 3 cut(s) 146, 689, 709
BfoI RGCGCY 1 cut(s) 549
BfuI GTATCC 1 cut(s) 831
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 5 cut(s) 108, 174, 183, 585, 774
BlsI GCNGC 5 cut(s) 109, 175, 184, 586, 775
Bme1390I CCNGG 3 cut(s) 560, 608, 761
Bme18I GGWCC 3 cut(s) 223, 757, 763
BmeRI GACNNNNNGTC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 781
BmgT120I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
BmiI GGNNCC 6 cut(s) 225, 245, 437, 553, 764, 855
BmrFI CCNGG 3 cut(s) 560, 608, 761
BpiI GAAGAC 1 cut(s) 520
BpmI CTGGAG 2 cut(s) 82, 836
Bpu10I CCTNAGC 2 cut(s) 169, 332
BpuMI CCSGG 2 cut(s) 608, 761
Bsa29I ATCGAT 2 cut(s) 601, 635
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 4 cut(s) 105, 213, 465, 495
BsaI GGTCTC 1 cut(s) 709
BsaJI CCNNGG 1 cut(s) 688
BsaWI WCCGGW 1 cut(s) 432
Bsc4I CCNNNNNNNGG 4 cut(s) 432, 571, 650, 772
Bse118I RCCGGY 2 cut(s) 432, 536
Bse1I ACTGG 2 cut(s) 728, 872
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 1 cut(s) 560
BseCI ATCGAT 2 cut(s) 601, 635
BseDI CCNNGG 1 cut(s) 688
BseGI GGATG 1 cut(s) 281
BseLI CCNNNNNNNGG 4 cut(s) 432, 571, 650, 772
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 346, 666
BseNI ACTGG 2 cut(s) 728, 872
BseRI GAGGAG 1 cut(s) 687
Bsh1236I CGCG 3 cut(s) 70, 582, 797
BshFI GGCC 3 cut(s) 563, 713, 790
BshNI GGYRCC 1 cut(s) 435
BshTI ACCGGT 1 cut(s) 432
BshVI ATCGAT 2 cut(s) 601, 635
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 5 cut(s) 433, 537, 549, 607, 760
BslFI GGGAC 3 cut(s) 67, 242, 776
BslI CCNNNNNNNGG 4 cut(s) 432, 571, 650, 772
BsmAI GTCTC 3 cut(s) 146, 689, 709
BsmBI CGTCTC 2 cut(s) 146, 689
BsmFI GGGAC 3 cut(s) 67, 242, 776
BsnI GGCC 3 cut(s) 563, 713, 790
Bso31I GGTCTC 1 cut(s) 709
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 4 cut(s) 518, 598, 678, 853
Bsp19I CCATGG 1 cut(s) 688
BspANI GGCC 3 cut(s) 563, 713, 790
BspCNI CTCAG 2 cut(s) 345, 667
BspDI ATCGAT 2 cut(s) 601, 635
BspFNI CGCG 3 cut(s) 70, 582, 797
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 6 cut(s) 225, 245, 437, 553, 764, 855
BspPI GGATC 4 cut(s) 513, 673, 848, 861
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 435
BspTNI GGTCTC 1 cut(s) 709
BsrBI CCGCTC 2 cut(s) 176, 664
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 2 cut(s) 432, 536
BsrI ACTGG 2 cut(s) 728, 872
BssAI RCCGGY 2 cut(s) 432, 536
BssECI CCNNGG 1 cut(s) 688
BssMI GATC 4 cut(s) 518, 598, 678, 853
BssNI GRCGYC 4 cut(s) 105, 213, 465, 495
BssT1I CCWWGG 1 cut(s) 688
Bst2UI CCWGG 1 cut(s) 560
Bst4CI ACNGT 5 cut(s) 37, 130, 220, 368, 475
Bst6I CTCTTC 1 cut(s) 654
BstACI GRCGYC 4 cut(s) 105, 213, 465, 495
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 112, 538
BstDEI CTNAG 3 cut(s) 169, 332, 675
BstDSI CCRYGG 1 cut(s) 688
BstF5I GGATG 1 cut(s) 281
BstFNI CGCG 3 cut(s) 70, 582, 797
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 2 cut(s) 548, 584
BstKTI GATC 4 cut(s) 521, 601, 681, 856
BstMAI GTCTC 3 cut(s) 146, 689, 709
BstMBI GATC 4 cut(s) 518, 598, 678, 853
BstMWI GCNNNNNNNGC 6 cut(s) 116, 179, 182, 347, 560, 581
BstNI CCWGG 1 cut(s) 560
BstSCI CCNGG 3 cut(s) 558, 606, 759
BstUI CGCG 3 cut(s) 70, 582, 797
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 3 cut(s) 518, 678, 853
BstYI RGATCY 3 cut(s) 518, 678, 853
Bsu15I ATCGAT 2 cut(s) 601, 635
BsuI GTATCC 1 cut(s) 831
BsuRI GGCC 3 cut(s) 563, 713, 790
BsuTUI ATCGAT 2 cut(s) 601, 635
BtgI CCRYGG 1 cut(s) 688
BtsCI GGATG 1 cut(s) 281
BtsIMutI CAGTG 2 cut(s) 126, 216
Cac8I GCNNGC 2 cut(s) 112, 538
CciI TCATGA 1 cut(s) 144
CfoI GCGC 2 cut(s) 548, 584
Cfr10I RCCGGY 2 cut(s) 432, 536
Cfr13I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
ClaI ATCGAT 2 cut(s) 601, 635
CpoI CGGWCCG 1 cut(s) 757
CseI GACGC 2 cut(s) 76, 113
Csp6I GTAC 1 cut(s) 436
CspAI ACCGGT 1 cut(s) 432
CspI CGGWCCG 1 cut(s) 757
CviAII CATG 3 cut(s) 45, 145, 689
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 3 cut(s) 169, 332, 675
DpnI GATC 4 cut(s) 520, 600, 680, 855
DpnII GATC 4 cut(s) 518, 598, 678, 853
DraIII CACNNNGTG 1 cut(s) 571
DrdI GACNNNNNNGTC 3 cut(s) 40, 73, 128
DriI GACNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 3 cut(s) 40, 73, 128
Eam1104I CTCTTC 1 cut(s) 654
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 654
EciI GGCGGA 3 cut(s) 236, 328, 333
Ecl136II GAGCTC 1 cut(s) 695
Eco130I CCWWGG 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 697
Eco31I GGTCTC 1 cut(s) 709
Eco32I GATATC 1 cut(s) 633
Eco47I GGWCC 3 cut(s) 223, 757, 763
Eco53kI GAGCTC 1 cut(s) 695
Eco72I CACGTG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 781
EcoICRI GAGCTC 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 558
EcoRV GATATC 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 697
ErhI CCWWGG 1 cut(s) 688
Esp3I CGTCTC 2 cut(s) 146, 689
FaeI CATG 3 cut(s) 48, 148, 692
FaiI YATR 5 cut(s) 46, 146, 152, 450, 690
FaqI GGGAC 3 cut(s) 67, 242, 776
FatI CATG 3 cut(s) 44, 144, 688
FauI CCCGC 4 cut(s) 63, 397, 657, 802
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 5 cut(s) 108, 174, 183, 585, 774
FokI GGATG 1 cut(s) 268
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 5 cut(s) 108, 174, 183, 585, 774
GlaI GCGC 2 cut(s) 547, 583
GluI GCNGC 5 cut(s) 108, 174, 183, 585, 774
GsuI CTGGAG 2 cut(s) 82, 836
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 3 cut(s) 563, 713, 790
HapII CCGG 5 cut(s) 433, 537, 549, 607, 760
HgaI GACGC 2 cut(s) 76, 113
HhaI GCGC 2 cut(s) 548, 584
Hin1I GRCGYC 4 cut(s) 105, 213, 465, 495
Hin1II CATG 3 cut(s) 48, 148, 692
Hin6I GCGC 2 cut(s) 546, 582
HinP1I GCGC 2 cut(s) 546, 582
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 6 cut(s) 17, 277, 321, 525, 603, 671
HpaII CCGG 5 cut(s) 433, 537, 549, 607, 760
HphI GGTGA 2 cut(s) 193, 316
Hpy166II GTNNAC 5 cut(s) 223, 236, 276, 364, 802
Hpy188I TCNGA 5 cut(s) 194, 211, 676, 757, 825
Hpy188III TCNNGA 4 cut(s) 145, 522, 613, 815
Hpy8I GTNNAC 5 cut(s) 223, 236, 276, 364, 802
HpyAV CCTTC 3 cut(s) 95, 337, 450
HpyCH4III ACNGT 5 cut(s) 37, 130, 220, 368, 475
HpyCH4IV ACGT 6 cut(s) 41, 213, 269, 465, 495, 570
HpyCH4V TGCA 1 cut(s) 512
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 179, 182, 347, 560, 581
HpyF3I CTNAG 3 cut(s) 169, 332, 675
HpySE526I ACGT 6 cut(s) 41, 213, 269, 465, 495, 570
Hsp92I GRCGYC 4 cut(s) 105, 213, 465, 495
Hsp92II CATG 3 cut(s) 48, 148, 692
HspAI GCGC 2 cut(s) 546, 582
KpnI GGTACC 1 cut(s) 439
KroI GCCGGC 1 cut(s) 536
KroNI GCCGGC 1 cut(s) 538
Kzo9I GATC 4 cut(s) 518, 598, 678, 853
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 5 cut(s) 181, 341, 551, 692, 700
MaeII ACGT 6 cut(s) 41, 213, 269, 465, 495, 570
MaeIII GTNAC 4 cut(s) 37, 97, 199, 214
MalI GATC 4 cut(s) 520, 600, 680, 855
MbiI CCGCTC 2 cut(s) 176, 664
MboI GATC 4 cut(s) 518, 598, 678, 853
MboII GAAGA 3 cut(s) 281, 520, 671
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 3 cut(s) 518, 678, 853
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 2 cut(s) 133, 383
MlyI GAGTC 3 cut(s) 271, 534, 680
MmeI TCCRAC 2 cut(s) 234, 735
MnlI CCTC 8 cut(s) 57, 317, 341, 352, 428, 488, 509, 708
MroNI GCCGGC 1 cut(s) 536
MspA1I CMGCKG 2 cut(s) 60, 392
MspI CCGG 5 cut(s) 433, 537, 549, 607, 760
MspR9I CCNGG 3 cut(s) 560, 608, 761
MunI CAATTG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 560
MvnI CGCG 3 cut(s) 70, 582, 797
MwoI GCNNNNNNNGC 6 cut(s) 116, 179, 182, 347, 560, 581
NaeI GCCGGC 1 cut(s) 538
NciI CCSGG 2 cut(s) 608, 761
NcoI CCATGG 1 cut(s) 688
NdeII GATC 4 cut(s) 518, 598, 678, 853
NgoMIV GCCGGC 1 cut(s) 536
NlaIII CATG 3 cut(s) 48, 148, 692
NlaIV GGNNCC 6 cut(s) 225, 245, 437, 553, 764, 855
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 2 cut(s) 199, 214
PaeR7I CTCGAG 1 cut(s) 781
PagI TCATGA 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 9 cut(s) 74, 272, 405, 459, 462, 471, 486, 586, 788
PdiI GCCGGC 1 cut(s) 538
PfeI GAWTC 3 cut(s) 17, 321, 603
PflFI GACNNNGTC 2 cut(s) 271, 494
PflMI CCANNNNNTGG 1 cut(s) 571
PinAI ACCGGT 1 cut(s) 432
PkrI GCNGC 5 cut(s) 109, 175, 184, 586, 775
PleI GAGTC 3 cut(s) 271, 533, 679
PmaCI CACGTG 1 cut(s) 571
PmlI CACGTG 1 cut(s) 571
PpsI GAGTC 3 cut(s) 271, 533, 679
Ppu21I YACGTR 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 558
PspCI CACGTG 1 cut(s) 571
PspGI CCWGG 1 cut(s) 558
PspN4I GGNNCC 6 cut(s) 225, 245, 437, 553, 764, 855
PspPI GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
PspXI VCTCGAGB 1 cut(s) 781
PsuI RGATCY 3 cut(s) 518, 678, 853
PsyI GACNNNGTC 2 cut(s) 271, 494
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
Rsr2I CGGWCCG 1 cut(s) 757
RsrII CGGWCCG 1 cut(s) 757
SacI GAGCTC 1 cut(s) 697
SalI GTCGAC 1 cut(s) 274
SapI GCTCTTC 1 cut(s) 654
SatI GCNGC 5 cut(s) 108, 174, 183, 585, 774
Sau3AI GATC 4 cut(s) 518, 598, 678, 853
Sau96I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
SchI GAGTC 3 cut(s) 271, 534, 680
ScrFI CCNGG 3 cut(s) 560, 608, 761
SduI GDGCHC 1 cut(s) 697
Sfr274I CTCGAG 1 cut(s) 781
SinI GGWCC 3 cut(s) 223, 757, 763
SlaI CTCGAG 1 cut(s) 781
SmlI CTYRAG 1 cut(s) 781
SmoI CTYRAG 1 cut(s) 781
Sse9I AATT 2 cut(s) 133, 383
SstI GAGCTC 1 cut(s) 697
StyD4I CCNGG 3 cut(s) 558, 606, 759
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 5 cut(s) 37, 130, 220, 368, 475
TaiI ACGT 6 cut(s) 44, 216, 272, 468, 498, 573
TaqI TCGA 9 cut(s) 156, 275, 408, 462, 480, 601, 635, 669, 782
TasI AATT 2 cut(s) 133, 383
TauI GCSGC 5 cut(s) 110, 176, 185, 587, 776
TfiI GAWTC 3 cut(s) 17, 321, 603
TscAI CASTG 2 cut(s) 133, 223
TseFI GTSAC 2 cut(s) 199, 214
Tsp45I GTSAC 2 cut(s) 199, 214
TspDTI ATGAA 2 cut(s) 161, 178
TspGWI ACGGA 2 cut(s) 671, 734
TspRI CASTG 2 cut(s) 133, 223
Tth111I GACNNNGTC 2 cut(s) 271, 494
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 3 cut(s) 223, 757, 763
XapI RAATTY 1 cut(s) 383
XhoI CTCGAG 1 cut(s) 781
XmiI GTMKAC 1 cut(s) 275
ZraI GACGTC 3 cut(s) 214, 466, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.