RchiOBHm_Chr5g0062251

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000184
Physical Location & Seq
Reverse (-)
67770699 .. 67772226
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33855

Sequence Viewer

Length: 912 bp
ATGTCCGCCGCCGTTAGTAGAAAGAATCCGTTGCAGAATCTTCCATGGAAGACCTGCAGGCTACTCATGGAGTTCGGATCCCCCCTCTTCCGCGCCGCAATGCGGCCCAACATGACCCTCAACCGCCGCCTCTTGACCCTTTCCGACCCTAAAGCCGCCGCTTTTTCCAAACCCAAAAACGGCGTCGTCTCCTTCGACGCCGCCGTCGACCCATCCCGCAACGTCTGGTTCCGCCTCTTCACCACCACCACCAACACCTCCGCCAAGCTCCCCGTCATCTTCTTCATCCACGGCGGCTCCTACATCTTCGGCGTCGCCGACTCCTTCGGCCTCGACTCCTCCTACAGCCGCCTCGCACGCCAGTTCTCCGCCGCCTCCTGCCGCCGCCTCGCACGCCAGCTCTCAGCCGCCGTCGTCTCCGTCAACTACCGCCTCTCGCCGGAGCACAAGTTTCCCTCCCAGCTCCGCGACGCCCTCGACTGCTTGAAATACCTCGACGATCACCACAGTGACGACGCATTCAGCTGTGCTGACGTCAGCCGGTGCTTCGTGGTGGGAGAGAGCGCGGGAGGGAACCTGGCCCACCACGTGGCGCTGGAAGCCGGAGAGCTGGAGTTGAAGAAGGTGAGGGTGGTGGGGCTCGTTTCGATTCAGCCGTTCTTCGGCGCAGAGGAGAGAACCGAATCCGAGATCCGTTTGGGTCGGGGCCCGCTGGGGTTGGACTCTACGGACTGGTGTTGGCCCACTTGGCGGGCTGTTTTACCGGACGGGCCGGACAGAGACCACCCGTTTGTGAATGTTTCGGGGCCCAATGCGGTGGACATTTCGGGTCGTGGGTACCCGGATACGCTTTGTTTTATTGGGGGTTGGATTCGTGGAGGGATTGGGGGAGGAGGTACTGTGAGTGGTTGA

Protein Analysis

303

Amino Acids

33.01

Weight (kDa)

8.87

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 84 - 205 2.5e-14 BD-FAE
Abhydrolase_3 PF07859 93 - 288 4e-35 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AatII GACGTC 1 cut(s) 537
Acc36I ACCTGC 1 cut(s) 62
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 837
AccB7I CCANNNNNTGG 1 cut(s) 589
AccI GTMKAC 1 cut(s) 207
AccII CGCG 3 cut(s) 93, 468, 566
AclWI GGATC 3 cut(s) 72, 85, 685
AcvI CACGTG 1 cut(s) 589
AcyI GRCGYC 5 cut(s) 183, 198, 312, 471, 534
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 2 cut(s) 839, 898
AfiI CCNNNNNNNGG 6 cut(s) 102, 179, 439, 589, 662, 750
AgsI TTSAA 2 cut(s) 487, 619
AjnI CCWGG 1 cut(s) 576
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 5 cut(s) 268, 400, 463, 525, 610
AluI AGCT 5 cut(s) 268, 400, 463, 525, 610
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 3 cut(s) 193, 421, 774
AlwI GGATC 3 cut(s) 72, 85, 685
AoxI GGCC 7 cut(s) 104, 328, 579, 706, 740, 770, 806
ApaI GGGCCC 2 cut(s) 710, 810
ArsI GACNNNNNNTTYG 4 cut(s) 168, 200, 773, 805
Asp718I GGTACC 1 cut(s) 837
AspLEI GCGC 4 cut(s) 95, 566, 595, 668
AspS9I GGNCC 8 cut(s) 105, 580, 706, 707, 741, 770, 806, 807
AsuC2I CCSGG 1 cut(s) 842
AsuHPI GGTGA 3 cut(s) 232, 494, 637
BaeGI GKGCMC 2 cut(s) 710, 810
BamHI GGATCC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 837
BanII GRGCYC 3 cut(s) 642, 710, 810
BbrPI CACGTG 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 447
BccI CCATC 1 cut(s) 220
BceAI ACGGC 5 cut(s) 188, 196, 307, 395, 640
BciT130I CCWGG 1 cut(s) 578
BciVI GTATCC 1 cut(s) 838
BcnI CCSGG 1 cut(s) 842
BcoDI GTCTC 3 cut(s) 193, 421, 774
BfmI CTRYAG 2 cut(s) 55, 343
BfoI RGCGCY 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 62
BfuI GTATCC 1 cut(s) 838
BglI GCCNNNNNGGC 1 cut(s) 748
Bme1390I CCNGG 2 cut(s) 578, 842
BmgT120I GGNCC 8 cut(s) 105, 580, 706, 707, 741, 770, 806, 807
BmiI GGNNCC 9 cut(s) 79, 230, 298, 575, 707, 708, 807, 808, 839
BmrFI CCNGG 2 cut(s) 578, 842
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 842
BsaAI YACGTR 1 cut(s) 589
BsaHI GRCGYC 5 cut(s) 183, 198, 312, 471, 534
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 2 cut(s) 44, 289
BsaWI WCCGGW 1 cut(s) 763
BsaXI ACNNNNNCTCC 5 cut(s) 281, 311, 434, 464, 885
Bsc4I CCNNNNNNNGG 6 cut(s) 102, 179, 439, 589, 662, 750
Bse118I RCCGGY 1 cut(s) 540
Bse1I ACTGG 2 cut(s) 361, 737
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 2 cut(s) 44, 289
BseGI GGATG 2 cut(s) 212, 285
BseLI CCNNNNNNNGG 6 cut(s) 102, 179, 439, 589, 662, 750
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 361, 737
BseRI GAGGAG 3 cut(s) 328, 686, 906
BseSI GKGCMC 2 cut(s) 710, 810
BseYI CCCAGC 2 cut(s) 459, 712
Bsh1236I CGCG 3 cut(s) 93, 468, 566
BshFI GGCC 7 cut(s) 106, 330, 581, 708, 742, 772, 808
BshNI GGYRCC 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 447
BsiSI CCGG 6 cut(s) 440, 541, 603, 764, 773, 842
BslI CCNNNNNNNGG 6 cut(s) 102, 179, 439, 589, 662, 750
BsmAI GTCTC 3 cut(s) 193, 421, 774
BsmBI CGTCTC 2 cut(s) 193, 421
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 7 cut(s) 106, 330, 581, 708, 742, 772, 808
Bso31I GGTCTC 1 cut(s) 774
Bsp120I GGGCCC 2 cut(s) 706, 806
Bsp1286I GDGCHC 4 cut(s) 447, 642, 710, 810
Bsp143I GATC 3 cut(s) 77, 499, 690
Bsp19I CCATGG 1 cut(s) 44
BspANI GGCC 7 cut(s) 106, 330, 581, 708, 742, 772, 808
BspCNI CTCAG 1 cut(s) 416
BspFNI CGCG 3 cut(s) 93, 468, 566
BspLI GGNNCC 9 cut(s) 79, 230, 298, 575, 707, 708, 807, 808, 839
BspMAI CTGCAG 1 cut(s) 59
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 3 cut(s) 72, 85, 685
BspT107I GGYRCC 1 cut(s) 837
BspTNI GGTCTC 1 cut(s) 774
BsrDI GCAATG 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 540
BsrI ACTGG 2 cut(s) 361, 737
BssAI RCCGGY 1 cut(s) 540
BssECI CCNNGG 2 cut(s) 44, 289
BssMI GATC 3 cut(s) 77, 499, 690
BssNI GRCGYC 5 cut(s) 183, 198, 312, 471, 534
BssT1I CCWWGG 1 cut(s) 44
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 509, 901
Bst6I CTCTTC 2 cut(s) 92, 242
BstACI GRCGYC 5 cut(s) 183, 198, 312, 471, 534
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 6 cut(s) 59, 358, 394, 398, 710, 753
BstDEI CTNAG 1 cut(s) 403
BstDSI CCRYGG 2 cut(s) 44, 289
BstF5I GGATG 2 cut(s) 212, 285
BstFNI CGCG 3 cut(s) 93, 468, 566
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 4 cut(s) 95, 566, 595, 668
BstKTI GATC 3 cut(s) 80, 502, 693
BstMAI GTCTC 3 cut(s) 193, 421, 774
BstMBI GATC 3 cut(s) 77, 499, 690
BstMWI GCNNNNNNNGC 4 cut(s) 357, 393, 599, 748
BstNI CCWGG 1 cut(s) 578
BstSCI CCNGG 2 cut(s) 576, 840
BstSFI CTRYAG 2 cut(s) 55, 343
BstSLI GKGCMC 2 cut(s) 710, 810
BstUI CGCG 3 cut(s) 93, 468, 566
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 77, 690
BstXI CCANNNNNNTGG 1 cut(s) 817
BstYI RGATCY 2 cut(s) 77, 690
BsuI GTATCC 1 cut(s) 838
BsuRI GGCC 7 cut(s) 106, 330, 581, 708, 742, 772, 808
BtgI CCRYGG 2 cut(s) 44, 289
BtsCI GGATG 2 cut(s) 212, 285
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 6 cut(s) 59, 358, 394, 398, 710, 753
CfoI GCGC 4 cut(s) 95, 566, 595, 668
Cfr10I RCCGGY 1 cut(s) 540
Cfr13I GGNCC 8 cut(s) 105, 580, 706, 707, 741, 770, 806, 807
CseI GACGC 5 cut(s) 172, 206, 301, 479, 524
Csp6I GTAC 2 cut(s) 838, 897
CviAII CATG 3 cut(s) 45, 67, 112
CviQI GTAC 2 cut(s) 838, 897
DdeI CTNAG 1 cut(s) 403
DpnI GATC 3 cut(s) 79, 501, 692
DpnII GATC 3 cut(s) 77, 499, 690
DraIII CACNNNGTG 1 cut(s) 589
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 2 cut(s) 92, 242
EarI CTCTTC 2 cut(s) 92, 242
EciI GGCGGA 3 cut(s) 221, 250, 358
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 3 cut(s) 642, 710, 810
Eco31I GGTCTC 1 cut(s) 774
Eco72I CACGTG 1 cut(s) 589
EcoO109I RGGNCCY 2 cut(s) 706, 806
EcoRII CCWGG 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 44
EcoT38I GRGCYC 3 cut(s) 642, 710, 810
ErhI CCWWGG 1 cut(s) 44
Esp3I CGTCTC 2 cut(s) 193, 421
FaeI CATG 3 cut(s) 48, 70, 115
FaiI YATR 3 cut(s) 46, 68, 113
FatI CATG 3 cut(s) 44, 66, 111
FauI CCCGC 4 cut(s) 224, 559, 717, 744
FblI GTMKAC 1 cut(s) 207
FokI GGATG 2 cut(s) 199, 272
FriOI GRGCYC 3 cut(s) 642, 710, 810
GlaI GCGC 4 cut(s) 94, 565, 594, 667
GsaI CCCAGC 2 cut(s) 463, 716
GsuI CTGGAG 1 cut(s) 632
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 7 cut(s) 106, 330, 581, 708, 742, 772, 808
HapII CCGG 6 cut(s) 440, 541, 603, 764, 773, 842
HgaI GACGC 5 cut(s) 172, 206, 301, 479, 524
HhaI GCGC 4 cut(s) 95, 566, 595, 668
Hin1I GRCGYC 5 cut(s) 183, 198, 312, 471, 534
Hin1II CATG 3 cut(s) 48, 70, 115
Hin6I GCGC 4 cut(s) 93, 564, 593, 666
HinP1I GCGC 4 cut(s) 93, 564, 593, 666
HincII GTYRAC 2 cut(s) 208, 424
HindII GTYRAC 2 cut(s) 208, 424
HinfI GANTC 8 cut(s) 25, 37, 320, 335, 649, 683, 722, 871
HpaII CCGG 6 cut(s) 440, 541, 603, 764, 773, 842
HphI GGTGA 3 cut(s) 232, 494, 637
Hpy166II GTNNAC 3 cut(s) 208, 424, 820
Hpy188I TCNGA 3 cut(s) 77, 145, 688
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 3 cut(s) 208, 424, 820
Hpy99I CGWCG 8 cut(s) 188, 200, 209, 317, 416, 473, 500, 518
HpyAV CCTTC 3 cut(s) 202, 334, 616
HpyCH4III ACNGT 2 cut(s) 509, 901
HpyCH4IV ACGT 3 cut(s) 222, 534, 588
HpyCH4V TGCA 2 cut(s) 34, 57
HpyF10VI GCNNNNNNNGC 4 cut(s) 357, 393, 599, 748
HpyF3I CTNAG 1 cut(s) 403
HpySE526I ACGT 3 cut(s) 222, 534, 588
Hsp92I GRCGYC 5 cut(s) 183, 198, 312, 471, 534
Hsp92II CATG 3 cut(s) 48, 70, 115
HspAI GCGC 4 cut(s) 93, 564, 593, 666
KpnI GGTACC 1 cut(s) 841
Kzo9I GATC 3 cut(s) 77, 499, 690
LmnI GCTCC 4 cut(s) 273, 302, 442, 468
MaeII ACGT 3 cut(s) 222, 534, 588
MaeIII GTNAC 1 cut(s) 509
MalI GATC 3 cut(s) 79, 501, 692
MboI GATC 3 cut(s) 77, 499, 690
MboII GAAGA 9 cut(s) 32, 61, 79, 229, 271, 274, 298, 631, 652
MflI RGATCY 2 cut(s) 77, 690
MhlI GDGCHC 4 cut(s) 447, 642, 710, 810
MlyI GAGTC 3 cut(s) 314, 329, 716
MmeI TCCRAC 3 cut(s) 168, 699, 848
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 2 cut(s) 525, 712
MspI CCGG 6 cut(s) 440, 541, 603, 764, 773, 842
MspR9I CCNGG 2 cut(s) 578, 842
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 1 cut(s) 578
MvnI CGCG 3 cut(s) 93, 468, 566
MwoI GCNNNNNNNGC 4 cut(s) 357, 393, 599, 748
NciI CCSGG 1 cut(s) 842
NcoI CCATGG 1 cut(s) 44
NdeII GATC 3 cut(s) 77, 499, 690
NlaIII CATG 3 cut(s) 48, 70, 115
NlaIV GGNNCC 9 cut(s) 79, 230, 298, 575, 707, 708, 807, 808, 839
NmuCI GTSAC 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 507
PcsI WCGNNNNNNNCGW 5 cut(s) 192, 201, 204, 315, 653
PctI GAATGC 1 cut(s) 518
PfeI GAWTC 5 cut(s) 25, 37, 649, 683, 871
PflMI CCANNNNNTGG 1 cut(s) 589
PleI GAGTC 3 cut(s) 314, 329, 716
PmaCI CACGTG 1 cut(s) 589
PmlI CACGTG 1 cut(s) 589
PpsI GAGTC 3 cut(s) 314, 329, 716
Ppu21I YACGTR 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 576
PspCI CACGTG 1 cut(s) 589
PspFI CCCAGC 2 cut(s) 459, 712
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 9 cut(s) 79, 230, 298, 575, 707, 708, 807, 808, 839
PspOMI GGGCCC 2 cut(s) 706, 806
PspPI GGNCC 8 cut(s) 105, 580, 706, 707, 741, 770, 806, 807
PstI CTGCAG 1 cut(s) 59
PsuI RGATCY 2 cut(s) 77, 690
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 2 cut(s) 839, 898
RsaNI GTAC 2 cut(s) 838, 897
RseI CAYNNNNRTG 1 cut(s) 507
SalI GTCGAC 1 cut(s) 206
Sau3AI GATC 3 cut(s) 77, 499, 690
Sau96I GGNCC 8 cut(s) 105, 580, 706, 707, 741, 770, 806, 807
SbfI CCTGCAGG 1 cut(s) 59
SchI GAGTC 3 cut(s) 314, 329, 716
ScrFI CCNGG 2 cut(s) 578, 842
SdaI CCTGCAGG 1 cut(s) 59
SduI GDGCHC 4 cut(s) 447, 642, 710, 810
SfcI CTRYAG 2 cut(s) 55, 343
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse8387I CCTGCAGG 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 576, 840
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 2 cut(s) 509, 901
TaiI ACGT 3 cut(s) 225, 537, 591
TaqI TCGA 6 cut(s) 195, 207, 333, 477, 495, 647
TfiI GAWTC 5 cut(s) 25, 37, 649, 683, 871
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 509
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 1 cut(s) 274
TspGWI ACGGA 4 cut(s) 18, 409, 683, 743
TspRI CASTG 1 cut(s) 514
Van91I CCANNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 207
ZraI GACGTC 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.