RchiOBHm_Chr6g0250061

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000184
Physical Location & Seq
Forward (+)
5756291 .. 5758317
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22416

Sequence Viewer

Length: 567 bp
ATGCATGTTGATACTTCCATCAAAGAATTGGAACGTCTTCTTTCCTTTCTTCAAAAGTTTAGAGAAACTGGATTTGAGGAGTCCTTGATTGAAGCTAAAGAGATTGCAAGTGAGATGGGGATTGAACCGGTATTCATGGAAAAACGGACAATTCACAAAAAGAGACAATTTGGTGAAAGCTCTAGCGAAGCGGTGACACAATCAGCTGCAGAATCCTTTAAAGTCAATTATTTTCTCTACATAGTTGATCAAGCTATTTCTTCATTCAAAACCAGGGCCGGCCCTGCCGCCGACCCTTCCCGCAACCTCTGGTTTCGCCTCTACACTCCCACCGCCGCTACCACCAAGCTCCCCATCATCATCTTCTTCCACGGCGGCGGGTTCGCCTTCATGTCCGCCGCTTCAAAACCCTACGACGACTTCTGTCAACGCCTCGCTCGCGAGATCCCCGCCGTCGTCGTCTCCGTCAACTACGACGACGGTTTCGACGTCCTCAAAGCAGCTTGTTCGAGGGCGCGAATCTCGGGTAGTGTTTTCTGGCCGGGGACAGCTCCTGCGGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

188

Amino Acids

20.91

Weight (kDa)

6.84

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 106 - 158 5.6e-06 Carboxylesterase family
Abhydrolase_3 PF07859 120 - 159 3.1e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccII CGCG 2 cut(s) 441, 517
AclWI GGATC 1 cut(s) 439
AcoI YGGCCR 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 489
AgeI ACCGGT 1 cut(s) 127
AgsI TTSAA 5 cut(s) 53, 92, 125, 268, 405
AjnI CCWGG 1 cut(s) 272
AluBI AGCT 7 cut(s) 95, 180, 206, 254, 349, 503, 551
AluI AGCT 7 cut(s) 95, 180, 206, 254, 349, 503, 551
Alw26I GTCTC 2 cut(s) 157, 466
AlwI GGATC 1 cut(s) 439
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 3 cut(s) 276, 280, 539
ApeKI GCWGC 2 cut(s) 206, 500
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AsiGI ACCGGT 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 2 cut(s) 276, 281
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 185, 205
AvaI CYCGRG 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 2 cut(s) 193, 512
BccI CCATC 3 cut(s) 26, 109, 362
BceAI ACGGC 2 cut(s) 388, 437
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 1 cut(s) 274
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 157, 466
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 6 cut(s) 207, 288, 336, 376, 399, 501
BlsI GCNGC 6 cut(s) 208, 289, 337, 377, 400, 502
Bme1390I CCNGG 2 cut(s) 274, 543
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 276, 281
BmrFI CCNGG 2 cut(s) 274, 543
BoxI GACNNNNGTC 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 273, 370, 542
BsaWI WCCGGW 1 cut(s) 127
Bse118I RCCGGY 2 cut(s) 127, 278
Bse1I ACTGG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 3 cut(s) 273, 370, 542
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 193, 512
Bsh1236I CGCG 2 cut(s) 441, 517
BshFI GGCC 3 cut(s) 278, 282, 541
BshTI ACCGGT 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 3 cut(s) 128, 279, 542
BslFI GGGAC 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 157, 466
BsmBI CGTCTC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 559
BsnI GGCC 3 cut(s) 278, 282, 541
BsoBI CYCGRG 1 cut(s) 523
Bsp143I GATC 2 cut(s) 247, 444
Bsp68I TCGCGA 1 cut(s) 441
BspANI GGCC 3 cut(s) 278, 282, 541
BspFNI CGCG 2 cut(s) 441, 517
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 439
BsrFI RCCGGY 2 cut(s) 127, 278
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 2 cut(s) 127, 278
BssECI CCNNGG 3 cut(s) 273, 370, 542
BssMI GATC 2 cut(s) 247, 444
BssNI GRCGYC 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 482
BstACI GRCGYC 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 280, 439
BstDSI CCRYGG 1 cut(s) 370
BstFNI CGCG 2 cut(s) 441, 517
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 2 cut(s) 250, 447
BstMAI GTCTC 2 cut(s) 157, 466
BstMBI GATC 2 cut(s) 247, 444
BstMWI GCNNNNNNNGC 2 cut(s) 284, 438
BstNI CCWGG 1 cut(s) 274
BstNSI RCATGY 1 cut(s) 8
BstPAI GACNNNNGTC 1 cut(s) 423
BstSCI CCNGG 2 cut(s) 272, 541
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 2 cut(s) 441, 517
BstV1I GCAGC 2 cut(s) 193, 512
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
BsuRI GGCC 3 cut(s) 278, 282, 541
BtgI CCRYGG 1 cut(s) 370
BtuMI TCGCGA 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 280, 439
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 1 cut(s) 517
Cfr10I RCCGGY 2 cut(s) 127, 278
Cfr13I GGNCC 2 cut(s) 276, 281
CspAI ACCGGT 1 cut(s) 127
CviAII CATG 3 cut(s) 5, 136, 391
DpnI GATC 2 cut(s) 249, 446
DpnII GATC 2 cut(s) 247, 444
DraI TTTAAA 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 539
EciI GGCGGA 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 272
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 3 cut(s) 8, 139, 394
FaiI YATR 5 cut(s) 6, 137, 242, 392, 565
FaqI GGGAC 1 cut(s) 559
FatI CATG 3 cut(s) 4, 135, 390
FauI CCCGC 4 cut(s) 308, 371, 457, 550
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 6 cut(s) 207, 288, 336, 376, 399, 501
FseI GGCCGGCC 1 cut(s) 282
Fsp4HI GCNGC 6 cut(s) 207, 288, 336, 376, 399, 501
FspBI CTAG 1 cut(s) 183
GlaI GCGC 1 cut(s) 516
GluI GCNGC 6 cut(s) 207, 288, 336, 376, 399, 501
HaeIII GGCC 3 cut(s) 278, 282, 541
HapII CCGG 3 cut(s) 128, 279, 542
HhaI GCGC 1 cut(s) 517
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 3 cut(s) 8, 139, 394
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HincII GTYRAC 2 cut(s) 428, 469
HindII GTYRAC 2 cut(s) 428, 469
HinfI GANTC 3 cut(s) 80, 212, 519
HpaII CCGG 3 cut(s) 128, 279, 542
HphI GGTGA 2 cut(s) 185, 205
Hpy166II GTNNAC 2 cut(s) 428, 469
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 2 cut(s) 428, 469
Hpy99I CGWCG 6 cut(s) 419, 458, 461, 479, 482, 491
HpyAV CCTTC 2 cut(s) 306, 397
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 2 cut(s) 34, 489
HpyCH4V TGCA 3 cut(s) 4, 107, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 438
HpySE526I ACGT 2 cut(s) 34, 489
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 3 cut(s) 8, 139, 394
HspAI GCGC 1 cut(s) 515
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 247, 444
LmnI GCTCC 2 cut(s) 354, 556
LpnPI CCDG 9 cut(s) 54, 141, 259, 286, 292, 295, 297, 523, 555
Lsp1109I GCAGC 2 cut(s) 193, 512
MaeI CTAG 1 cut(s) 183
MaeII ACGT 2 cut(s) 34, 489
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 249, 446
MboI GATC 2 cut(s) 247, 444
MboII GAAGA 5 cut(s) 29, 41, 252, 355, 358
MflI RGATCY 1 cut(s) 444
MluCI AATT 4 cut(s) 26, 150, 167, 226
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 6 cut(s) 70, 317, 329, 443, 503, 504
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 36
MseI TTAA 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 3 cut(s) 128, 279, 542
MspR9I CCNGG 2 cut(s) 274, 543
MvaI CCWGG 1 cut(s) 274
MvnI CGCG 2 cut(s) 441, 517
MwoI GCNNNNNNNGC 2 cut(s) 284, 438
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 1 cut(s) 543
NdeII GATC 2 cut(s) 247, 444
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 3 cut(s) 8, 139, 394
NmuCI GTSAC 1 cut(s) 193
NruI TCGCGA 1 cut(s) 441
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PcsI WCGNNNNNNNCGW 3 cut(s) 462, 483, 486
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 212, 519
PinAI ACCGGT 1 cut(s) 127
PkrI GCNGC 6 cut(s) 208, 289, 337, 377, 400, 502
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PshAI GACNNNNGTC 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 272
PspGI CCWGG 1 cut(s) 272
PspPI GGNCC 2 cut(s) 276, 281
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 444
PvuII CAGCTG 1 cut(s) 206
RigI GGCCGGCC 1 cut(s) 282
RruI TCGCGA 1 cut(s) 441
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 6 cut(s) 207, 288, 336, 376, 399, 501
Sau3AI GATC 2 cut(s) 247, 444
Sau96I GGNCC 2 cut(s) 276, 281
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 274, 543
SfcI CTRYAG 1 cut(s) 207
Sse9I AATT 4 cut(s) 26, 150, 167, 226
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 272, 541
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 2 cut(s) 37, 492
TaqI TCGA 2 cut(s) 486, 509
TasI AATT 4 cut(s) 26, 150, 167, 226
TauI GCSGC 4 cut(s) 290, 338, 378, 401
TfiI GAWTC 2 cut(s) 212, 519
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 2 cut(s) 206, 500
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 3 cut(s) 124, 252, 379
TspGWI ACGGA 2 cut(s) 160, 454
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 1 cut(s) 183
ZraI GACGTC 1 cut(s) 490
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.