RchiOBHm_Chr6g0250041

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000184
Physical Location & Seq
Forward (+)
5730282 .. 5730827
546 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22415

Sequence Viewer

Length: 546 bp
ATGGATCGTCCGCCTCTTCGTCTCCGCCTCTTCCTTCGCAATCGACACCTCTCGCCACACAAACCTCACCGTCGTCTCTTTAACTTGACCGACTACAAACCATCTCCCTTAACAAAACCTCACAAAAACCTCAAGAGCTCTGACGTCGTCGTTGTCGCCAACCCTTCCTGCAACCTCCGGTTCCGCCTCTACATTCCCACCGCTGCTACCACCAAGCTCCCCATCATCGTCTTCTTCCACGGCGGCGGGTTTGTCTTCATGTCCGCCACTTCAAAACCCTACGACGACTTCTGTCAACTCCTCACTTGGGTGCCCCCCGCCGTCGTCATCTCCTTCAACTACAACCTCGCACTGGAGCATCAGTATCCGTGCCAATACGACGACAGTTTAGACATCCTCAAGTTCATCGAAGACAGCAGCTTGTTCGAGGGCGCGAATCTCGGGCAGTGCTTTCTGGTCGGGGATTGCGCCAGTGAGAACATAGCGCACCACGTGGCGATTAGAGCGAGCGGGCACGAGTTTTGGGAGCTGAATGTTGGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

181

Amino Acids

20.6

Weight (kDa)

7.72

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 19 - 116 5.7e-06 Carboxylesterase family
BD-FAE PF20434 63 - 163 6.4e-07 BD-FAE
Abhydrolase_3 PF07859 76 - 172 5e-22 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
AccB1I GGYRCC 1 cut(s) 310
AccBSI CCGCTC 1 cut(s) 510
AccII CGCG 1 cut(s) 434
AciI CCGC 9 cut(s) 11, 25, 184, 201, 243, 246, 264, 318, 510
AclWI GGATC 1 cut(s) 12
AcvI CACGTG 1 cut(s) 493
AcyI GRCGYC 1 cut(s) 144
AdeI CACNNNGTG 1 cut(s) 493
AfiI CCNNNNNNNGG 2 cut(s) 307, 352
AgsI TTSAA 2 cut(s) 273, 337
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 4 cut(s) 138, 217, 420, 529
AluI AGCT 4 cut(s) 138, 217, 420, 529
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 2 cut(s) 26, 80
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 203, 417
AspLEI GCGC 3 cut(s) 434, 470, 487
AsuHPI GGTGA 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 440
BaeGI GKGCMC 2 cut(s) 315, 516
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 140
BauI CACGAG 1 cut(s) 515
BbrPI CACGTG 1 cut(s) 493
BbsI GAAGAC 3 cut(s) 223, 247, 417
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 190, 429
BccI CCATC 2 cut(s) 109, 230
BceAI ACGGC 2 cut(s) 256, 305
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 26, 80
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 3 cut(s) 204, 244, 418
BlsI GCNGC 3 cut(s) 205, 245, 419
BmeT110I CYCGRG 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 182, 312
BmsI GCATC 1 cut(s) 367
BoxI GACNNNNGTC 1 cut(s) 291
BpiI GAAGAC 3 cut(s) 223, 247, 417
BpmI CTGGAG 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 116, 383
BsaAI YACGTR 1 cut(s) 493
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 352
Bse1I ACTGG 2 cut(s) 357, 471
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 307, 352
BseNI ACTGG 2 cut(s) 357, 471
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 2 cut(s) 315, 516
BseXI GCAGC 2 cut(s) 190, 429
Bsh1236I CGCG 1 cut(s) 434
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 307, 352
BsmAI GTCTC 2 cut(s) 26, 80
BsmBI CGTCTC 2 cut(s) 26, 80
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 140, 315, 516
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 9 cut(s) 11, 25, 184, 201, 243, 246, 264, 318, 510
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 2 cut(s) 182, 312
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 510
BsrI ACTGG 2 cut(s) 357, 471
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 515
Bst2BI CACGAG 1 cut(s) 515
Bst4CI ACNGT 2 cut(s) 71, 386
Bst6I CTCTTC 2 cut(s) 21, 35
BstACI GRCGYC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 508, 512
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 3 cut(s) 434, 470, 487
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 2 cut(s) 26, 80
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstPAI GACNNNNGTC 1 cut(s) 291
BstSLI GKGCMC 2 cut(s) 315, 516
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 2 cut(s) 190, 429
BstV2I GAAGAC 3 cut(s) 223, 247, 417
BsuI GTATCC 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 393
BtsI GCAGTG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 350, 452, 478
Cac8I GCNNGC 2 cut(s) 508, 512
CfoI GCGC 3 cut(s) 434, 470, 487
CviAII CATG 1 cut(s) 259
CviJI RGCY 4 cut(s) 138, 217, 420, 529
CviKI_1 RGCY 4 cut(s) 138, 217, 420, 529
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
DraIII CACNNNGTG 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 21, 35
EarI CTCTTC 2 cut(s) 21, 35
EciI GGCGGA 3 cut(s) 14, 173, 253
Ecl136II GAGCTC 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 140
Eco53kI GAGCTC 1 cut(s) 138
Eco72I CACGTG 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 140
Esp3I CGTCTC 2 cut(s) 26, 80
FaeI CATG 1 cut(s) 262
FaiI YATR 2 cut(s) 260, 482
FatI CATG 1 cut(s) 258
FauI CCCGC 3 cut(s) 239, 325, 503
Fnu4HI GCNGC 3 cut(s) 204, 244, 418
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 204, 244, 418
GlaI GCGC 3 cut(s) 433, 469, 486
GluI GCNGC 3 cut(s) 204, 244, 418
GsuI CTGGAG 1 cut(s) 374
HapII CCGG 1 cut(s) 178
HhaI GCGC 3 cut(s) 434, 470, 487
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 3 cut(s) 432, 468, 485
HinP1I GCGC 3 cut(s) 432, 468, 485
HincII GTYRAC 1 cut(s) 296
HindII GTYRAC 1 cut(s) 296
HinfI GANTC 1 cut(s) 436
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 296
Hpy99I CGWCG 6 cut(s) 75, 149, 152, 287, 326, 383
HpyAV CCTTC 3 cut(s) 44, 174, 343
HpyCH4III ACNGT 2 cut(s) 71, 386
HpyCH4IV ACGT 2 cut(s) 144, 492
HpyCH4V TGCA 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpySE526I ACGT 2 cut(s) 144, 492
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 3 cut(s) 432, 468, 485
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 3 cut(s) 222, 355, 526
LpnPI CCDG 5 cut(s) 181, 191, 338, 440, 484
Lsp1109I GCAGC 2 cut(s) 190, 429
LweI GCATC 1 cut(s) 367
MaeII ACGT 2 cut(s) 144, 492
MalI GATC 1 cut(s) 6
MbiI CCGCTC 1 cut(s) 510
MboI GATC 1 cut(s) 4
MboII GAAGA 6 cut(s) 8, 22, 223, 226, 247, 422
MhlI GDGCHC 3 cut(s) 140, 315, 516
MseI TTAA 3 cut(s) 81, 110, 544
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 503
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 2 cut(s) 182, 312
OliI CACNNNNGTG 1 cut(s) 308
PfeI GAWTC 1 cut(s) 436
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 3 cut(s) 205, 245, 419
PmaCI CACGTG 1 cut(s) 493
PmlI CACGTG 1 cut(s) 493
Ppu21I YACGTR 1 cut(s) 493
PshAI GACNNNNGTC 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 140
PspCI CACGTG 1 cut(s) 493
PspN4I GGNNCC 2 cut(s) 182, 312
PsyI GACNNNGTC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 308
SacI GAGCTC 1 cut(s) 140
SaqAI TTAA 3 cut(s) 81, 110, 544
SatI GCNGC 3 cut(s) 204, 244, 418
Sau3AI GATC 1 cut(s) 4
SduI GDGCHC 3 cut(s) 140, 315, 516
SfaNI GCATC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 2 cut(s) 131, 398
SmoI CTYRAG 2 cut(s) 131, 398
SsiI CCGC 9 cut(s) 11, 25, 184, 201, 243, 246, 264, 318, 510
SstI GAGCTC 1 cut(s) 140
TaaI ACNGT 2 cut(s) 71, 386
TaiI ACGT 2 cut(s) 147, 495
TaqI TCGA 3 cut(s) 43, 408, 426
TaqII GACCGA 1 cut(s) 104
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 3 cut(s) 81, 110, 544
Tru9I TTAA 3 cut(s) 81, 110, 544
TscAI CASTG 3 cut(s) 357, 452, 478
TseI GCWGC 2 cut(s) 203, 417
TspDTI ATGAA 2 cut(s) 247, 394
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 3 cut(s) 357, 452, 478
Tth111I GACNNNGTC 1 cut(s) 146
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.