MD03G1173100.v1.1

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000184
Physical Location & Seq
Reverse (-)
23590192 .. 23590767
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1173100.v1.1.491

Sequence Viewer

Length: 576 bp
ATGAGCCTATTCGACTTCAAAGCCTTGCCTTCGGACAAACCCAACAACGCGGTCAAAACCCCCGACGTAATGGTGGACCCTTCCCGCAACCTCTGGTTCCGCCTCTACGTCCCCACCACCACCGACCCTGCCTCCAAGCTCCCACTCGTTATCTACTTCCACGGTGGTGGATTCGTGTTCTTTTCTGCCAACTCACAACCCTACAACGACCTCTGCAAACGTCTCGCCACCAAACTCCCTGCCGTCATCATCTCCATCAACTACCGCCATGCGCCGGAGCATCGGTATCCTTCCCAATACGAAGACAGCATGGACGTCCTCAAATTCATTGACACAACAAGAATTGAGGGTCTTGATCTCAACAACGTTGACATCACCCGGTGTTTCCTCGCCGGAGACAGCGCTGGCGGCAACCTGGCCCACCACGTGGTAATCAAGACGAGCGAGCATGAGTTTTGCCAGATGAGGGTGATGGGACTAATAGCGATACAGCCATGTTTTTGGCGGGAAAGAAAGGACCGAATCGGAAACAAGGCTTGCAAAAGAGGGCTTGGCGATCACTTTGGAGCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

192

Amino Acids

21.59

Weight (kDa)

8.27

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Say1_Mug180 PF10340 28 - 151 2.9e-08 Steryl acetyl hydrolase
COesterase PF00135 34 - 90 8.2e-07 Carboxylesterase family
BD-FAE PF20434 35 - 152 7.3e-14 BD-FAE
Abhydrolase_3 PF07859 50 - 169 2e-34 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 318
AccB7I CCANNNNNTGG 1 cut(s) 427
AccII CGCG 1 cut(s) 50
AciI CCGC 6 cut(s) 50, 85, 100, 265, 408, 505
AclI AACGTT 1 cut(s) 366
AcsI RAATTY 1 cut(s) 323
AcvI CACGTG 1 cut(s) 427
AcyI GRCGYC 1 cut(s) 315
AdeI CACNNNGTG 2 cut(s) 381, 427
AfeI AGCGCT 1 cut(s) 403
AfiI CCNNNNNNNGG 3 cut(s) 274, 427, 466
AgsI TTSAA 1 cut(s) 19
AjnI CCWGG 1 cut(s) 414
AleI CACNNNNGTG 1 cut(s) 165
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 227, 390
Aor51HI AGCGCT 1 cut(s) 403
AoxI GGCC 1 cut(s) 417
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 2 cut(s) 274, 404
AspS9I GGNCC 3 cut(s) 76, 418, 517
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 2 cut(s) 367, 481
AvaII GGWCC 2 cut(s) 76, 517
BbrPI CACGTG 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 309
BccI CCATC 2 cut(s) 263, 466
BceAI ACGGC 1 cut(s) 227
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 416
BciVI GTATCC 1 cut(s) 297
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 227, 390
BfoI RGCGCY 1 cut(s) 405
BfuI GTATCC 1 cut(s) 297
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 379, 416
Bme18I GGWCC 2 cut(s) 76, 517
BmgT120I GGNCC 3 cut(s) 76, 418, 517
BmiI GGNNCC 2 cut(s) 78, 98
BmrFI CCNGG 2 cut(s) 379, 416
BmsI GCATC 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 427
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 269, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 427, 466
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 160
BseLI CCNNNNNNNGG 3 cut(s) 274, 427, 466
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 1 cut(s) 419
BsiSI CCGG 3 cut(s) 275, 379, 393
BslFI GGGAC 2 cut(s) 95, 489
BslI CCNNNNNNNGG 3 cut(s) 274, 427, 466
BsmAI GTCTC 2 cut(s) 227, 390
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 95, 489
BsnI GGCC 1 cut(s) 419
Bsp143I GATC 2 cut(s) 355, 556
BspACI CCGC 6 cut(s) 50, 85, 100, 265, 408, 505
BspANI GGCC 1 cut(s) 419
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 78, 98
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 355, 556
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 406, 446, 538
BstDSI CCRYGG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 2 cut(s) 274, 404
BstKTI GATC 2 cut(s) 358, 559
BstMAI GTCTC 2 cut(s) 227, 390
BstMBI GATC 2 cut(s) 355, 556
BstMWI GCNNNNNNNGC 1 cut(s) 408
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 377, 414
BstUI CGCG 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 309
BstXI CCANNNNNNTGG 2 cut(s) 167, 501
BsuI GTATCC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 419
BtgI CCRYGG 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 406, 446, 538
CfoI GCGC 2 cut(s) 274, 404
Cfr13I GGNCC 3 cut(s) 76, 418, 517
CviAII CATG 4 cut(s) 269, 310, 449, 495
CviJI RGCY 7 cut(s) 6, 23, 139, 419, 493, 536, 550
CviKI_1 RGCY 7 cut(s) 6, 23, 139, 419, 493, 536, 550
DpnI GATC 2 cut(s) 357, 558
DpnII GATC 2 cut(s) 355, 556
DraIII CACNNNGTG 2 cut(s) 381, 427
EciI GGCGGA 1 cut(s) 89
Eco47I GGWCC 2 cut(s) 76, 517
Eco47III AGCGCT 1 cut(s) 403
Eco72I CACGTG 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 4 cut(s) 272, 313, 452, 498
FaiI YATR 4 cut(s) 270, 311, 450, 496
FaqI GGGAC 2 cut(s) 95, 489
FatI CATG 4 cut(s) 268, 309, 448, 494
FauI CCCGC 2 cut(s) 92, 498
Fnu4HI GCNGC 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 409
GlaI GCGC 2 cut(s) 273, 403
GluI GCNGC 1 cut(s) 409
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 419
HapII CCGG 3 cut(s) 275, 379, 393
HhaI GCGC 2 cut(s) 274, 404
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 4 cut(s) 272, 313, 452, 498
Hin6I GCGC 2 cut(s) 272, 402
HinP1I GCGC 2 cut(s) 272, 402
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 2 cut(s) 171, 522
HpaII CCGG 3 cut(s) 275, 379, 393
HphI GGTGA 2 cut(s) 367, 481
Hpy166II GTNNAC 2 cut(s) 76, 370
Hpy188I TCNGA 2 cut(s) 34, 527
Hpy188III TCNNGA 2 cut(s) 353, 436
Hpy8I GTNNAC 2 cut(s) 76, 370
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 39, 90, 300
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 6 cut(s) 66, 108, 220, 315, 366, 426
HpyCH4V TGCA 2 cut(s) 216, 540
HpyF10VI GCNNNNNNNGC 1 cut(s) 408
HpySE526I ACGT 6 cut(s) 66, 108, 220, 315, 366, 426
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 4 cut(s) 272, 313, 452, 498
HspAI GCGC 2 cut(s) 272, 402
Kzo9I GATC 2 cut(s) 355, 556
LmnI GCTCC 3 cut(s) 144, 277, 566
LweI GCATC 1 cut(s) 289
MaeII ACGT 6 cut(s) 66, 108, 220, 315, 366, 426
MalI GATC 2 cut(s) 357, 558
MboI GATC 2 cut(s) 355, 556
MboII GAAGA 1 cut(s) 314
MluCI AATT 2 cut(s) 323, 342
MnlI CCTC 9 cut(s) 101, 113, 142, 221, 329, 340, 398, 459, 539
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 3 cut(s) 275, 379, 393
MspR9I CCNGG 2 cut(s) 379, 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 408
NciI CCSGG 1 cut(s) 379
NdeII GATC 2 cut(s) 355, 556
NlaIII CATG 4 cut(s) 272, 313, 452, 498
NlaIV GGNNCC 2 cut(s) 78, 98
OliI CACNNNNGTG 1 cut(s) 165
PfeI GAWTC 2 cut(s) 171, 522
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 1 cut(s) 410
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
Ppu21I YACGTR 1 cut(s) 427
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 414
PspCI CACGTG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 78, 98
PspPI GGNCC 3 cut(s) 76, 418, 517
RseI CAYNNNNRTG 1 cut(s) 165
SatI GCNGC 1 cut(s) 409
Sau3AI GATC 2 cut(s) 355, 556
Sau96I GGNCC 3 cut(s) 76, 418, 517
ScrFI CCNGG 2 cut(s) 379, 416
SfaNI GCATC 1 cut(s) 289
SinI GGWCC 2 cut(s) 76, 517
SmiMI CAYNNNNRTG 1 cut(s) 165
Sse9I AATT 2 cut(s) 323, 342
SsiI CCGC 6 cut(s) 50, 85, 100, 265, 408, 505
StyD4I CCNGG 2 cut(s) 377, 414
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 6 cut(s) 69, 111, 223, 318, 369, 429
TaqI TCGA 1 cut(s) 12
TaqII GACCGA 1 cut(s) 534
TasI AATT 2 cut(s) 323, 342
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 2 cut(s) 171, 522
TspDTI ATGAA 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 2 cut(s) 76, 517
XapI RAATTY 1 cut(s) 323
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.