Rh3DG119700

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000184
Physical Location & Seq
Reverse (-)
10064945 .. 10065526
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG119700.1

Sequence Viewer

Length: 582 bp
ATGAGCTTCCTCGACTTACACTCCTCCGCCACTCCCTCAGCCCCCTACAAAGGCGTCACCACCTCCGACGTCACCGTCGACCAAACCCGCAACCTCTGGTTCCTCTTCTTCGTCCCTTCTACGACGTCGTCTTCCCCCGCCTCTTTACCCGTCGTCGTTTTCTTCCACGGCGGCGGCTTCAGCTTTCTCAGCCCCGACTCCTCCTACGCCTACGACGCCGTCTGCCGCAAATTGGCCCGCGAAATCCCCGCCGTCGTAGTCTCCGTCAACTACCACCTCTCCCCAGAGCACGGCTACCCCTGCCAGTACGACGACGGCCTCGACAAATTCCTCGACCAAAACCTCGACTCCCTCCTCGACCTCGCTGACTGCTCCCGCTGCTTCCTCGCCGGAGACAGCGCCGGCGCAAACATTGCCCACCACGTGGCGGTGCGAGCCTGCAGCGAGCCGTTTCGGGTTGTGAAACCAATCGGGTTGATATCAATCCAGAAAACCCAGAAACCCGGTGGCTTTCTTGAGACTGTGGAAGGCTTTCTTGCCACAAAACCCAGAAACCCGGTGGCTTCATTGAGACTGAGGTGA

Protein Analysis

193

Amino Acids

20.94

Weight (kDa)

6.28

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 37 - 94 9.1e-07 Carboxylesterase family
BD-FAE PF20434 46 - 145 1e-07 BD-FAE
Abhydrolase_3 PF07859 52 - 153 3.3e-30 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 2 cut(s) 72, 128
AccB7I CCANNNNNTGG 1 cut(s) 424
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 240
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 163
AcvI CACGTG 1 cut(s) 424
AcyI GRCGYC 4 cut(s) 54, 69, 125, 216
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 5 cut(s) 50, 232, 290, 424, 427
AluBI AGCT 2 cut(s) 6, 183
AluI AGCT 2 cut(s) 6, 183
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 4 cut(s) 265, 387, 512, 565
AoxI GGCC 2 cut(s) 234, 316
ApeKI GCWGC 2 cut(s) 378, 441
ApoI RAATTY 1 cut(s) 326
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 2 cut(s) 401, 407
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 504, 557
AsuHPI GGTGA 2 cut(s) 49, 64
BbrPI CACGTG 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 365, 453
BceAI ACGGC 6 cut(s) 184, 203, 236, 307, 331, 433
BcnI CCSGG 2 cut(s) 504, 557
BcoDI GTCTC 4 cut(s) 265, 387, 512, 565
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 5 cut(s) 172, 175, 226, 379, 442
BlsI GCNGC 5 cut(s) 173, 176, 227, 380, 443
Bme1390I CCNGG 2 cut(s) 504, 557
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 504, 557
BpiI GAAGAC 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 2 cut(s) 504, 557
BsaAI YACGTR 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 482
BsaHI GRCGYC 4 cut(s) 54, 69, 125, 216
BsaJI CCNNGG 1 cut(s) 166
BsaXI ACNNNNNCTCC 6 cut(s) 5, 35, 263, 293, 332, 362
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 232, 290, 424, 427
Bse118I RCCGGY 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 411
Bse8I GATNNNNATC 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 166
BseJI GATNNNNATC 1 cut(s) 482
BseLI CCNNNNNNNGG 5 cut(s) 50, 232, 290, 424, 427
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 51, 202, 566
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 3 cut(s) 13, 190, 344
BseXI GCAGC 2 cut(s) 365, 453
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 2 cut(s) 236, 318
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 4 cut(s) 390, 402, 504, 557
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 5 cut(s) 50, 232, 290, 424, 427
BsmAI GTCTC 4 cut(s) 265, 387, 512, 565
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 2 cut(s) 236, 318
Bsp1286I GDGCHC 1 cut(s) 291
BspANI GGCC 2 cut(s) 236, 318
BspCNI CTCAG 3 cut(s) 50, 201, 567
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 401
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 166
BssNI GRCGYC 4 cut(s) 54, 69, 125, 216
Bst4CI ACNGT 2 cut(s) 76, 523
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 4 cut(s) 54, 69, 125, 216
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 238, 403, 435, 439, 446
BstDEI CTNAG 3 cut(s) 37, 188, 575
BstDSI CCRYGG 1 cut(s) 166
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 2 cut(s) 401, 407
BstMAI GTCTC 4 cut(s) 265, 387, 512, 565
BstMWI GCNNNNNNNGC 7 cut(s) 180, 189, 215, 300, 378, 413, 434
BstSCI CCNGG 2 cut(s) 502, 555
BstSFI CTRYAG 1 cut(s) 439
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 365, 453
BstV2I GAAGAC 1 cut(s) 123
BsuRI GGCC 2 cut(s) 236, 318
BtgI CCRYGG 1 cut(s) 166
Cac8I GCNNGC 5 cut(s) 238, 403, 435, 439, 446
CfoI GCGC 2 cut(s) 401, 407
Cfr10I RCCGGY 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 2 cut(s) 43, 224
Csp6I GTAC 1 cut(s) 307
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 3 cut(s) 37, 188, 575
DraIII CACNNNGTG 1 cut(s) 424
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 163
Eco72I CACGTG 1 cut(s) 424
EcoRV GATATC 1 cut(s) 480
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 98
FauI CCCGC 5 cut(s) 95, 145, 245, 256, 383
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 172, 175, 226, 379, 442
Fsp4HI GCNGC 5 cut(s) 172, 175, 226, 379, 442
GlaI GCGC 2 cut(s) 400, 406
GluI GCNGC 5 cut(s) 172, 175, 226, 379, 442
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 2 cut(s) 236, 318
HapII CCGG 4 cut(s) 390, 402, 504, 557
HgaI GACGC 2 cut(s) 43, 224
HhaI GCGC 2 cut(s) 401, 407
Hin1I GRCGYC 4 cut(s) 54, 69, 125, 216
Hin6I GCGC 2 cut(s) 399, 405
HinP1I GCGC 2 cut(s) 399, 405
HincII GTYRAC 2 cut(s) 79, 268
HindII GTYRAC 2 cut(s) 79, 268
HinfI GANTC 2 cut(s) 197, 347
HpaII CCGG 4 cut(s) 390, 402, 504, 557
HphI GGTGA 2 cut(s) 49, 64
Hpy166II GTNNAC 2 cut(s) 79, 268
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 2 cut(s) 487, 515
Hpy8I GTNNAC 2 cut(s) 79, 268
HpyAV CCTTC 2 cut(s) 126, 521
HpyCH4III ACNGT 2 cut(s) 76, 523
HpyCH4IV ACGT 3 cut(s) 69, 125, 423
HpyCH4V TGCA 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 189, 215, 300, 378, 413, 434
HpyF3I CTNAG 3 cut(s) 37, 188, 575
HpySE526I ACGT 3 cut(s) 69, 125, 423
Hsp92I GRCGYC 4 cut(s) 54, 69, 125, 216
HspAI GCGC 2 cut(s) 399, 405
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 377
Lsp1109I GCAGC 2 cut(s) 365, 453
MaeII ACGT 3 cut(s) 69, 125, 423
MaeIII GTNAC 2 cut(s) 55, 70
MboII GAAGA 4 cut(s) 97, 100, 123, 154
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 2 cut(s) 230, 326
MlyI GAGTC 2 cut(s) 191, 341
MmeI TCCRAC 1 cut(s) 90
MreI CGCCGGCG 1 cut(s) 401
MroNI GCCGGC 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 4 cut(s) 390, 402, 504, 557
MspR9I CCNGG 2 cut(s) 504, 557
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 7 cut(s) 180, 189, 215, 300, 378, 413, 434
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 2 cut(s) 504, 557
NgoMIV GCCGGC 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 2 cut(s) 55, 70
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 261, 318
PdiI GCCGGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 531
PflFI GACNNNGTC 2 cut(s) 127, 218
PflMI CCANNNNNTGG 1 cut(s) 424
PkrI GCNGC 5 cut(s) 173, 176, 227, 380, 443
PleI GAGTC 2 cut(s) 191, 341
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 2 cut(s) 191, 341
Ppu21I YACGTR 1 cut(s) 424
PspCI CACGTG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 235
PstI CTGCAG 1 cut(s) 443
PsyI GACNNNGTC 2 cut(s) 127, 218
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
SalI GTCGAC 1 cut(s) 77
SatI GCNGC 5 cut(s) 172, 175, 226, 379, 442
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 191, 341
ScrFI CCNGG 2 cut(s) 504, 557
SduI GDGCHC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 439
SgrAI CRCCGGYG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 2 cut(s) 230, 326
StyD4I CCNGG 2 cut(s) 502, 555
TaaI ACNGT 2 cut(s) 76, 523
TaiI ACGT 3 cut(s) 72, 128, 426
TaqI TCGA 6 cut(s) 12, 78, 321, 333, 345, 357
TasI AATT 2 cut(s) 230, 326
TauI GCSGC 3 cut(s) 174, 177, 228
TseFI GTSAC 2 cut(s) 55, 70
TseI GCWGC 2 cut(s) 378, 441
Tsp45I GTSAC 2 cut(s) 55, 70
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 1 cut(s) 253
Tth111I GACNNNGTC 2 cut(s) 127, 218
Van91I CCANNNNNTGG 1 cut(s) 424
XapI RAATTY 1 cut(s) 326
XcmI CCANNNNNNNNNTGG 2 cut(s) 503, 556
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 531
ZraI GACGTC 2 cut(s) 70, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.