Rroxscaffold_1G00028440

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000184
Physical Location & Seq
Forward (+)
36229064 .. 36247877
18814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028440.1

Sequence Viewer

Length: 588 bp
ATGGCGGTGACAGCGAGCTCCAAAGGACAACGACTTGCTGCAATAAAAGAAGAGGAAAGTGATTGGCATGTGGCAATGACATGCATTTATCATTATCTTGACTTTCAACTGCTAGACCTCAACTGTGCCGACAAGCTAGCACACAAAGAAGGAGAAACAACAAAGATGACCCGAAACCCTCCAACATCGCCGGAACTTCCATGCACGCTCCGGTTCTTCCTCTTCATCCTCACCTTCGTTCTCAATACCGCTCGCCGCTCCAACGGCACGGTAAACCGCCCTTTTCTGAAAGTCTTCGACTCCAAGGCCTCTCCTTCAAACAAACGCGACGTCAAAACCACTGATGTAATGGTTGACCCCTCCCGCAATCTATGGTTCCGCCTCTTCGTCCCCACGACCACCACCACCACCAAACTCCCTGCGATAATATACTTCCATGGCGGCGGTTTCGTCTTCTTCTCTGCCAACTCAACTCTCTTCGAAGGCATCTGCAGACGACTCGCCCTGAACCTCCCTGCCATCGTCATCTCCGTCAACTACCGTCTCGCGCCGGAGTATCGCTACCCGTCGAAGACTTCGACGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

195

Amino Acids

22.07

Weight (kDa)

9.4

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 124 - 183 3.8e-06 Carboxylesterase family
Abhydrolase_3 PF07859 142 - 191 1.5e-15 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
AccBSI CCGCTC 2 cut(s) 251, 258
AccII CGCG 2 cut(s) 327, 548
AciI CCGC 8 cut(s) 5, 249, 256, 277, 364, 379, 441, 444
AcyI GRCGYC 1 cut(s) 330
AgsI TTSAA 2 cut(s) 107, 318
AjiI CACGTC 1 cut(s) 582
AluBI AGCT 2 cut(s) 18, 136
AluI AGCT 2 cut(s) 18, 136
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 1 cut(s) 548
AoxI GGCC 1 cut(s) 306
ApeKI GCWGC 1 cut(s) 38
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 19, 223
AsuII TTCGAA 1 cut(s) 480
AsuNHI GCTAGC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 3 cut(s) 286, 445, 578
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 527
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 2 cut(s) 113, 137
BfmI CTRYAG 1 cut(s) 490
BisI GCNGC 3 cut(s) 39, 256, 442
BlsI GCNGC 3 cut(s) 40, 257, 443
BmgBI CACGTC 1 cut(s) 582
BmiI GGNNCC 1 cut(s) 377
BmsI GCATC 1 cut(s) 495
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 3 cut(s) 286, 445, 578
Bpu14I TTCGAA 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 2 cut(s) 303, 436
BsaWI WCCGGW 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 303, 436
BseGI GGATG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 81
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 327, 548
BshFI GGCC 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 191, 211, 551
BslFI GGGAC 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 548
BsmBI CGTCTC 1 cut(s) 548
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 1 cut(s) 308
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 8 cut(s) 5, 249, 256, 277, 364, 379, 441, 444
BspANI GGCC 1 cut(s) 308
BspFNI CGCG 2 cut(s) 327, 548
BspLI GGNNCC 1 cut(s) 377
BspMAI CTGCAG 1 cut(s) 494
BspOI GCTAGC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 480
BsrBI CCGCTC 2 cut(s) 251, 258
BsrDI GCAATG 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 303, 436
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 2 cut(s) 303, 436
Bst4CI ACNGT 3 cut(s) 125, 271, 542
Bst6I CTCTTC 4 cut(s) 45, 227, 389, 482
BstACI GRCGYC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 4 cut(s) 16, 138, 206, 253
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 2 cut(s) 327, 548
BstHHI GCGC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 548
BstMWI GCNNNNNNNGC 2 cut(s) 11, 264
BstNSI RCATGY 2 cut(s) 71, 84
BstSFI CTRYAG 1 cut(s) 490
BstUI CGCG 2 cut(s) 327, 548
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 3 cut(s) 286, 445, 578
BsuRI GGCC 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 436
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 582
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 4 cut(s) 16, 138, 206, 253
CfoI GCGC 1 cut(s) 550
CviAII CATG 4 cut(s) 68, 81, 201, 437
CviJI RGCY 3 cut(s) 18, 136, 308
CviKI_1 RGCY 3 cut(s) 18, 136, 308
Eam1104I CTCTTC 4 cut(s) 45, 227, 389, 482
EarI CTCTTC 4 cut(s) 45, 227, 389, 482
EciI GGCGGA 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 303, 436
Eco147I AGGCCT 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 303, 436
EcoT22I ATGCAT 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 2 cut(s) 303, 436
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 4 cut(s) 71, 84, 204, 440
FaiI YATR 6 cut(s) 69, 82, 202, 373, 430, 438
FaqI GGGAC 1 cut(s) 374
FatI CATG 4 cut(s) 67, 80, 200, 436
FauI CCCGC 1 cut(s) 371
Fnu4HI GCNGC 3 cut(s) 39, 256, 442
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 39, 256, 442
FspBI CTAG 2 cut(s) 113, 137
GlaI GCGC 1 cut(s) 549
GluI GCNGC 3 cut(s) 39, 256, 442
HaeIII GGCC 1 cut(s) 308
HapII CCGG 3 cut(s) 191, 211, 551
HhaI GCGC 1 cut(s) 550
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 4 cut(s) 71, 84, 204, 440
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 2 cut(s) 355, 535
HindII GTYRAC 2 cut(s) 355, 535
HinfI GANTC 2 cut(s) 299, 498
HpaII CCGG 3 cut(s) 191, 211, 551
HphI GGTGA 2 cut(s) 19, 223
Hpy166II GTNNAC 3 cut(s) 274, 355, 535
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 3 cut(s) 274, 355, 535
Hpy99I CGWCG 3 cut(s) 332, 571, 583
HpyAV CCTTC 4 cut(s) 143, 244, 324, 476
HpyCH4III ACNGT 3 cut(s) 125, 271, 542
HpyCH4IV ACGT 2 cut(s) 330, 581
HpyCH4V TGCA 4 cut(s) 41, 84, 204, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 264
HpySE526I ACGT 2 cut(s) 330, 581
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 4 cut(s) 71, 84, 204, 440
HspAI GCGC 1 cut(s) 548
LmnI GCTCC 3 cut(s) 23, 213, 263
LpnPI CCDG 6 cut(s) 204, 224, 432, 518, 528, 564
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 1 cut(s) 495
MaeI CTAG 2 cut(s) 113, 137
MaeII ACGT 2 cut(s) 330, 581
MaeIII GTNAC 1 cut(s) 7
MbiI CCGCTC 2 cut(s) 251, 258
MboII GAAGA 9 cut(s) 62, 208, 214, 286, 376, 445, 448, 469, 583
MhlI GDGCHC 1 cut(s) 20
MlyI GAGTC 2 cut(s) 293, 492
MmeI TCCRAC 2 cut(s) 206, 285
MnlI CCTC 9 cut(s) 46, 128, 189, 230, 239, 319, 370, 392, 521
Mph1103I ATGCAT 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 293
MspI CCGG 3 cut(s) 191, 211, 551
MvnI CGCG 2 cut(s) 327, 548
MwoI GCNNNNNNNGC 2 cut(s) 11, 264
NcoI CCATGG 1 cut(s) 436
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 4 cut(s) 71, 84, 204, 440
NlaIV GGNNCC 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 7
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 2 cut(s) 71, 84
NspV TTCGAA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 308
PcsI WCGNNNNNNNCGW 2 cut(s) 528, 575
PdmI GAANNNNTTC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 40, 257, 443
PleI GAGTC 2 cut(s) 293, 492
PpsI GAGTC 2 cut(s) 293, 492
Psp124BI GAGCTC 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 377
PstI CTGCAG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 20
SatI GCNGC 3 cut(s) 39, 256, 442
SchI GAGTC 2 cut(s) 293, 492
SduI GDGCHC 1 cut(s) 20
SetI ASST 7 cut(s) 20, 120, 138, 236, 333, 513, 584
SfaNI GCATC 1 cut(s) 495
SfcI CTRYAG 1 cut(s) 490
SfuI TTCGAA 1 cut(s) 480
SseBI AGGCCT 1 cut(s) 308
SsiI CCGC 8 cut(s) 5, 249, 256, 277, 364, 379, 441, 444
SspMI CTAG 2 cut(s) 113, 137
SstI GAGCTC 1 cut(s) 20
StuI AGGCCT 1 cut(s) 308
StyI CCWWGG 2 cut(s) 303, 436
TaaI ACNGT 3 cut(s) 125, 271, 542
TaiI ACGT 2 cut(s) 333, 584
TaqI TCGA 4 cut(s) 297, 480, 569, 578
TauI GCSGC 2 cut(s) 258, 444
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 1 cut(s) 346
XceI RCATGY 2 cut(s) 71, 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 2 cut(s) 113, 137
ZraI GACGTC 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.