Rh6BG005200

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000184
Physical Location & Seq
Reverse (-)
757862 .. 780828
22967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG005200.1

Sequence Viewer

Length: 1065 bp
ATGGCCGAAATCCAAAGCTCACCAGATCTGCCATGGACCGTCCGCCTCTTCGTCTCCGCCGCTTCCTTCGCAGCCGACGCCTCTCGCCGCTCAAACTTCACCGTCAACCGTCGCCTCTTTAACTTGTTCGACTACAAACCCTCTCCCTCAACAAAACCTCACAAAAACCTCAAGAGCTCCGACGTCGTCGTCGCCGCTGACCCTTCCCGCAACCTCTGGTTCCTGCTCTACACTCCCACCGCCGCTACCACCAAGCTCCCCATCATCGTTTTCTTCCACGGCGGCGGGTTCGCCTTCATGTCTGCCGCTTCAAAACCCTACGACGACTTCTGTCAACGCCTCGCTCGCGAGATCCCCGCCGTCGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCATCGGTATCCGTGCCAGTACGATGACGGTTTCGACGTCCTCAAGTTCATCGACGACAGTCAAAGCAGCTTGTTCGAGGGCGCGAATCTCGCGCAGTGTTTTCTGGCAGGGGACAGCTCCGGCGGGAACATAGCGCACCACGTGGCGATCAGAGCGAGCGGACACGAGTTTCGGGATCTGAAGGTGGTCGGGATCTTGGCGATTCAGCCGTTTTTCGGCGGAGAAGAGCGGACGGAATCGGAGAAGAGACTGAAGAGTGTGCCGCTGGTGAACGTAGAGCGGACGGACTGGATGTGGAAGGCGTTTTTGCCGGAGGGGTCGGATCGGGACCACCCGGTGGTGAATGTGTTCGGGCCGAACGCGGTGGATATTTCGGGTGTGTATTTTCCGGCGACGATTGTGGTTGTTGGGGGATTTGATCCGTTGCAGGATTGGCAGAAGAGATACTACGAGGGATTGAAGAAACGCGGGAAACAAGCGGAGCTGTCTTCAAATATGCGAATCAGATACATAGCAGCAGTACTTGGGTCAAATGAACAGAAAATACAACAAGATCTAGAAGCAAGCGAGGGCGAGACTAAGAGAACTAGAGAAGTCTTAAGATTTAGTGAGAGAAGACTGGCTGAAAAAATGGGTGAGGATGGAACATGGATACCATTGCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

354

Amino Acids

39.72

Weight (kDa)

7.06

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 76 - 189 2.6e-12 BD-FAE
COesterase PF00135 76 - 130 5.5e-08 Carboxylesterase family
Abhydrolase_3 PF07859 89 - 294 4.8e-56 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AatII GACGTC 2 cut(s) 186, 435
AccB7I CCANNNNNTGG 1 cut(s) 733
AccBSI CCGCTC 4 cut(s) 90, 555, 625, 676
AccII CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
AclWI GGATC 5 cut(s) 346, 579, 596, 726, 809
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 596, 668
AcvI CACGTG 1 cut(s) 538
AcyI GRCGYC 3 cut(s) 78, 183, 432
AdeI CACNNNGTG 2 cut(s) 538, 733
AfaI GTAC 2 cut(s) 416, 918
AfiI CCNNNNNNNGG 3 cut(s) 391, 611, 733
AflII CTTAAG 1 cut(s) 994
AgsI TTSAA 3 cut(s) 312, 856, 888
AluBI AGCT 6 cut(s) 18, 177, 256, 465, 513, 880
AluI AGCT 6 cut(s) 18, 177, 256, 465, 513, 880
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 4 cut(s) 58, 373, 637, 965
AlwI GGATC 5 cut(s) 346, 579, 596, 726, 809
AoxI GGCC 2 cut(s) 3, 749
ApeKI GCWGC 3 cut(s) 71, 462, 911
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
Asp700I GAANNNNTTC 1 cut(s) 743
AspLEI GCGC 4 cut(s) 391, 479, 490, 532
AspS9I GGNCC 3 cut(s) 36, 724, 749
AsuC2I CCSGG 1 cut(s) 731
AsuHPI GGTGA 5 cut(s) 12, 91, 676, 748, 1043
AvaII GGWCC 2 cut(s) 36, 724
BanII GRGCYC 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BauI CACGAG 1 cut(s) 560
BbrPI CACGTG 1 cut(s) 538
BbsI GAAGAC 2 cut(s) 876, 1018
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 83, 474, 923
BccI CCATC 2 cut(s) 269, 1031
BceAI ACGGC 3 cut(s) 295, 344, 589
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciVI GTATCC 2 cut(s) 414, 1041
BcnI CCSGG 1 cut(s) 731
BcoDI GTCTC 4 cut(s) 58, 373, 637, 965
BfaI CTAG 2 cut(s) 953, 984
BfrI CTTAAG 1 cut(s) 994
BfuI GTATCC 2 cut(s) 414, 1041
BglII AGATCT 2 cut(s) 25, 949
BmcAI AGTACT 1 cut(s) 918
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 2 cut(s) 36, 724
BmgT120I GGNCC 3 cut(s) 36, 724, 749
BmiI GGNNCC 2 cut(s) 221, 725
BmrFI CCNGG 1 cut(s) 731
BmsI GCATC 1 cut(s) 406
BoxI GACNNNNGTC 2 cut(s) 330, 453
BpiI GAAGAC 2 cut(s) 876, 1018
BpuEI CTTGAG 2 cut(s) 155, 422
BpuMI CCSGG 1 cut(s) 731
BsaAI YACGTR 1 cut(s) 538
BsaHI GRCGYC 3 cut(s) 78, 183, 432
BsaJI CCNNGG 2 cut(s) 32, 277
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 611, 733
Bse1I ACTGG 3 cut(s) 412, 689, 1020
Bse3DI GCAATG 1 cut(s) 1052
BseDI CCNNGG 2 cut(s) 32, 277
BseGI GGATG 2 cut(s) 693, 1042
BseLI CCNNNNNNNGG 3 cut(s) 391, 611, 733
BseMI GCAATG 1 cut(s) 1052
BseNI ACTGG 3 cut(s) 412, 689, 1020
BseXI GCAGC 3 cut(s) 83, 474, 923
Bsh1236I CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
BshFI GGCC 2 cut(s) 5, 751
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 5 cut(s) 392, 516, 707, 731, 785
BslFI GGGAC 2 cut(s) 521, 737
BslI CCNNNNNNNGG 3 cut(s) 391, 611, 733
BsmAI GTCTC 4 cut(s) 58, 373, 637, 965
BsmBI CGTCTC 2 cut(s) 58, 373
BsmFI GGGAC 2 cut(s) 521, 737
BsnI GGCC 2 cut(s) 5, 751
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
Bsp19I CCATGG 1 cut(s) 32
Bsp68I TCGCGA 1 cut(s) 348
BspANI GGCC 2 cut(s) 5, 751
BspFNI CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
BspLI GGNNCC 2 cut(s) 221, 725
BspPI GGATC 5 cut(s) 346, 579, 596, 726, 809
BspQI GCTCTTC 1 cut(s) 615
BspTI CTTAAG 1 cut(s) 994
BsrBI CCGCTC 4 cut(s) 90, 555, 625, 676
BsrDI GCAATG 1 cut(s) 1052
BsrI ACTGG 3 cut(s) 412, 689, 1020
BssECI CCNNGG 2 cut(s) 32, 277
BssMI GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
BssNI GRCGYC 3 cut(s) 78, 183, 432
BssSI CACGAG 1 cut(s) 560
BssT1I CCWWGG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 560
Bst4CI ACNGT 5 cut(s) 40, 103, 110, 425, 455
Bst6I CTCTTC 5 cut(s) 53, 615, 635, 644, 830
BstACI GRCGYC 3 cut(s) 78, 183, 432
BstAFI CTTAAG 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 538
BstC8I GCNNGC 3 cut(s) 346, 553, 961
BstDEI CTNAG 1 cut(s) 975
BstDSI CCRYGG 2 cut(s) 32, 277
BstF5I GGATG 2 cut(s) 693, 1042
BstFNI CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
BstHHI GCGC 4 cut(s) 391, 479, 490, 532
BstKTI GATC 8 cut(s) 28, 354, 546, 574, 591, 721, 817, 952
BstMAI GTCTC 4 cut(s) 58, 373, 637, 965
BstMBI GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
BstMWI GCNNNNNNNGC 6 cut(s) 68, 77, 345, 485, 548, 829
BstPAI GACNNNNGTC 2 cut(s) 330, 453
BstSCI CCNGG 1 cut(s) 729
BstUI CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
BstV1I GCAGC 3 cut(s) 83, 474, 923
BstV2I GAAGAC 2 cut(s) 876, 1018
BstX2I RGATCY 5 cut(s) 25, 351, 571, 588, 949
BstYI RGATCY 5 cut(s) 25, 351, 571, 588, 949
BsuI GTATCC 2 cut(s) 414, 1041
BsuRI GGCC 2 cut(s) 5, 751
BtgI CCRYGG 2 cut(s) 32, 277
BtsCI GGATG 2 cut(s) 693, 1042
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 1 cut(s) 497
BtuMI TCGCGA 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 346, 553, 961
CfoI GCGC 4 cut(s) 391, 479, 490, 532
Cfr13I GGNCC 3 cut(s) 36, 724, 749
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 415, 917
CviAII CATG 3 cut(s) 33, 298, 1044
CviQI GTAC 2 cut(s) 415, 917
DdeI CTNAG 1 cut(s) 975
DpnI GATC 8 cut(s) 27, 353, 545, 573, 590, 720, 816, 951
DpnII GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
DraIII CACNNNGTG 2 cut(s) 538, 733
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 5 cut(s) 53, 615, 635, 644, 830
EarI CTCTTC 5 cut(s) 53, 615, 635, 644, 830
EciI GGCGGA 3 cut(s) 32, 46, 630
Ecl136II GAGCTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 36, 724
Eco53kI GAGCTC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 596, 668
Eco72I CACGTG 1 cut(s) 538
EcoICRI GAGCTC 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 32
Esp3I CGTCTC 2 cut(s) 58, 373
FaeI CATG 3 cut(s) 36, 301, 1047
FaiI YATR 6 cut(s) 34, 299, 527, 893, 908, 1045
FaqI GGGAC 2 cut(s) 521, 737
FatI CATG 3 cut(s) 32, 297, 1043
FauI CCCGC 5 cut(s) 215, 278, 364, 512, 857
FokI GGATG 2 cut(s) 700, 1049
FriOI GRGCYC 1 cut(s) 179
FspBI CTAG 2 cut(s) 953, 984
GlaI GCGC 4 cut(s) 390, 478, 489, 531
HaeIII GGCC 2 cut(s) 5, 751
HapII CCGG 5 cut(s) 392, 516, 707, 731, 785
HgaI GACGC 1 cut(s) 86
HhaI GCGC 4 cut(s) 391, 479, 490, 532
Hin1I GRCGYC 3 cut(s) 78, 183, 432
Hin1II CATG 3 cut(s) 36, 301, 1047
Hin6I GCGC 4 cut(s) 389, 477, 488, 530
HinP1I GCGC 4 cut(s) 389, 477, 488, 530
HincII GTYRAC 3 cut(s) 106, 335, 376
HindII GTYRAC 3 cut(s) 106, 335, 376
HinfI GANTC 4 cut(s) 481, 598, 632, 897
HpaII CCGG 5 cut(s) 392, 516, 707, 731, 785
HphI GGTGA 5 cut(s) 12, 91, 676, 748, 1043
Hpy166II GTNNAC 4 cut(s) 106, 335, 376, 667
Hpy188I TCNGA 6 cut(s) 181, 548, 576, 637, 718, 902
Hpy188III TCNNGA 6 cut(s) 172, 347, 569, 586, 722, 953
Hpy8I GTNNAC 4 cut(s) 106, 335, 376, 667
HpyAV CCTTC 5 cut(s) 76, 213, 304, 571, 688
HpyCH4III ACNGT 5 cut(s) 40, 103, 110, 425, 455
HpyCH4IV ACGT 4 cut(s) 183, 432, 537, 669
HpyCH4V TGCA 1 cut(s) 823
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 77, 345, 485, 548, 829
HpyF3I CTNAG 1 cut(s) 975
HpySE526I ACGT 4 cut(s) 183, 432, 537, 669
Hsp92I GRCGYC 3 cut(s) 78, 183, 432
Hsp92II CATG 3 cut(s) 36, 301, 1047
HspAI GCGC 4 cut(s) 389, 477, 488, 530
Kzo9I GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 5 cut(s) 182, 261, 394, 518, 877
Lsp1109I GCAGC 3 cut(s) 83, 474, 923
LweI GCATC 1 cut(s) 406
MaeI CTAG 2 cut(s) 953, 984
MaeII ACGT 4 cut(s) 183, 432, 537, 669
MalI GATC 8 cut(s) 27, 353, 545, 573, 590, 720, 816, 951
MbiI CCGCTC 4 cut(s) 90, 555, 625, 676
MboI GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
MboII GAAGA 9 cut(s) 40, 265, 632, 652, 661, 847, 868, 876, 1023
MflI RGATCY 5 cut(s) 25, 351, 571, 588, 949
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 1 cut(s) 1060
MmeI TCCRAC 2 cut(s) 204, 696
MroXI GAANNNNTTC 1 cut(s) 743
MseI TTAA 4 cut(s) 120, 995, 1059, 1063
MspA1I CMGCKG 2 cut(s) 197, 661
MspCI CTTAAG 1 cut(s) 994
MspI CCGG 5 cut(s) 392, 516, 707, 731, 785
MspR9I CCNGG 1 cut(s) 731
MvnI CGCG 6 cut(s) 348, 389, 479, 488, 758, 864
MwoI GCNNNNNNNGC 6 cut(s) 68, 77, 345, 485, 548, 829
NciI CCSGG 1 cut(s) 731
NcoI CCATGG 1 cut(s) 32
NdeII GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
NlaIII CATG 3 cut(s) 36, 301, 1047
NlaIV GGNNCC 2 cut(s) 221, 725
NruI TCGCGA 1 cut(s) 348
PacI TTAATTAA 1 cut(s) 1063
PciSI GCTCTTC 1 cut(s) 615
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 429
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 4 cut(s) 481, 598, 632, 897
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 733
PmaCI CACGTG 1 cut(s) 538
PmlI CACGTG 1 cut(s) 538
Ppu21I YACGTR 1 cut(s) 538
PshAI GACNNNNGTC 2 cut(s) 330, 453
Psp124BI GAGCTC 1 cut(s) 179
PspCI CACGTG 1 cut(s) 538
PspN4I GGNNCC 2 cut(s) 221, 725
PspPI GGNCC 3 cut(s) 36, 724, 749
PsrI GAACNNNNNNTAC 2 cut(s) 924, 956
PsuI RGATCY 5 cut(s) 25, 351, 571, 588, 949
PsyI GACNNNGTC 1 cut(s) 185
RruI TCGCGA 1 cut(s) 348
RsaI GTAC 2 cut(s) 416, 918
RsaNI GTAC 2 cut(s) 415, 917
SacI GAGCTC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 4 cut(s) 120, 995, 1059, 1063
Sau3AI GATC 8 cut(s) 25, 351, 543, 571, 588, 718, 814, 949
Sau96I GGNCC 3 cut(s) 36, 724, 749
ScaI AGTACT 1 cut(s) 918
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 1 cut(s) 406
SinI GGWCC 2 cut(s) 36, 724
SmlI CTYRAG 3 cut(s) 170, 437, 994
SmoI CTYRAG 3 cut(s) 170, 437, 994
Sse9I AATT 1 cut(s) 1060
SspMI CTAG 2 cut(s) 953, 984
SstI GAGCTC 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 729
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 5 cut(s) 40, 103, 110, 425, 455
TaiI ACGT 4 cut(s) 186, 435, 540, 672
TaqI TCGA 4 cut(s) 129, 429, 447, 471
TasI AATT 1 cut(s) 1060
TatI WGTACW 1 cut(s) 916
TauI GCSGC 7 cut(s) 62, 90, 197, 245, 285, 308, 661
TfiI GAWTC 4 cut(s) 481, 598, 632, 897
Tru1I TTAA 4 cut(s) 120, 995, 1059, 1063
Tru9I TTAA 4 cut(s) 120, 995, 1059, 1063
TscAI CASTG 1 cut(s) 497
TseI GCWGC 3 cut(s) 71, 462, 911
TspDTI ATGAA 3 cut(s) 286, 433, 945
TspGWI ACGGA 5 cut(s) 361, 396, 644, 695, 807
TspRI CASTG 1 cut(s) 497
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 994
VpaK11BI GGWCC 2 cut(s) 36, 724
XbaI TCTAGA 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 2 cut(s) 953, 984
ZraI GACGTC 2 cut(s) 184, 433
ZrmI AGTACT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.