RLG00000015547

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000184
Physical Location & Seq
Reverse (-)
67721499 .. 67722496
998 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015547

Sequence Viewer

Length: 828 bp
ATGTCCAAAGCTCACCAGATCTGCCATGGACAGTCCGCCTCTTCGTCTCCTCCGCTTCCTACGCAGCCGACGCCTCTCGCCGCTCAAACTTCACCGTCAACCGTCGCCTCTTTTAACTTGTTTTACTACAAACCCTCTCCCTTAACAAAACCTCACAAAAACCTCCAGAGCTCCGACGTCGTCGTCGCCGCTGACCCTTCCCGCAACCTCTGGTTCCGCCTCTACACTCCCACCGCCGCTACCACCAAGCTCCCCATCATCGTCTTCTTCCACGGCGGCGGGTTCGCCTTCATGTCTGCCGCTTCAAAACCCTACGACGACTTCTGTCAACGCCTCGCTCGCGAGATCCCCGCCGTCGTAGTCTCCGTCAACTACCGCCTCGCGCAGGAGCATCCGTATCCGTGCCAGTACGACGACGGTTTCGACGTCCTCAAGTTCATCGACGACAGTCAACGCAGCTTGTTCGAGGGCGCGAATCTCGTGCAGTGTTTTCTGGCCTGGGACAGCTCCGGCGGGAACATAGCGCACCACGTGGCGATCAGAGCGAGCGGGCACGAATTTCGGGAGCTGAAGGTGGTCGGAATGTTGGCGATTCAGCCGTTTTTCGGTGGAGAAGAGCGGACGGAATCGGAGAAGAGACTGAAGAGTGTGCCGCTGAAGAGATACTATGAGGGATTGAAGAAACGCGGGAAACAAGCGGAGCTAGTGGTGTTTCCAAATGCCATTCATGTTTCCAAGCGGAGTTGGACGACTCTTCTGCGTTTATTAACCGAGTCAGGGATTTCGTGCAATTGCAAGCAGTTAAATGTTACTGAAACAAGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

276

Amino Acids

30.65

Weight (kDa)

9.19

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 72 - 128 3.3e-08 Carboxylesterase family
BD-FAE PF20434 74 - 187 5.3e-11 BD-FAE
Abhydrolase_3 PF07859 87 - 213 3.2e-32 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 182
AatII GACGTC 2 cut(s) 180, 429
AccBSI CCGCTC 3 cut(s) 83, 549, 619
AccII CGCG 4 cut(s) 342, 383, 473, 687
AclWI GGATC 1 cut(s) 340
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 3 cut(s) 590, 662, 677
AcvI CACGTG 1 cut(s) 532
AcyI GRCGYC 3 cut(s) 71, 177, 426
AdeI CACNNNGTG 1 cut(s) 532
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 385, 605, 777
AgsI TTSAA 2 cut(s) 306, 679
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 7 cut(s) 11, 171, 250, 459, 507, 568, 703
AluI AGCT 7 cut(s) 11, 171, 250, 459, 507, 568, 703
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 3 cut(s) 51, 367, 631
AlwI GGATC 1 cut(s) 340
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 64, 456
ApoI RAATTY 1 cut(s) 557
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AspLEI GCGC 3 cut(s) 385, 473, 526
AsuHPI GGTGA 2 cut(s) 5, 84
BaeGI GKGCMC 1 cut(s) 555
BanII GRGCYC 1 cut(s) 173
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 76, 468
BccI CCATC 1 cut(s) 263
BceAI ACGGC 3 cut(s) 289, 338, 583
BcgI CGANNNNNNTGC 8 cut(s) 445, 463, 479, 497, 542, 576, 739, 773
BciT130I CCWGG 1 cut(s) 499
BciVI GTATCC 1 cut(s) 408
BcoDI GTCTC 3 cut(s) 51, 367, 631
BfaI CTAG 1 cut(s) 704
BfuI GTATCC 1 cut(s) 408
BglII AGATCT 1 cut(s) 18
BisI GCNGC 8 cut(s) 65, 81, 189, 237, 277, 300, 457, 653
BlsI GCNGC 8 cut(s) 66, 82, 190, 238, 278, 301, 458, 654
Bme1390I CCNGG 1 cut(s) 499
BmiI GGNNCC 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 400
BoxI GACNNNNGTC 2 cut(s) 324, 447
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 532
BsaHI GRCGYC 3 cut(s) 71, 177, 426
BsaJI CCNNGG 3 cut(s) 25, 271, 498
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 605, 777
Bse1I ACTGG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 3 cut(s) 25, 271, 498
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 3 cut(s) 385, 605, 777
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 2 cut(s) 76, 468
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 4 cut(s) 342, 383, 473, 687
BshFI GGCC 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 173
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 385, 605, 777
BsmAI GTCTC 3 cut(s) 51, 367, 631
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 1 cut(s) 497
Bsp1286I GDGCHC 2 cut(s) 173, 555
Bsp143I GATC 3 cut(s) 18, 345, 537
Bsp19I CCATGG 1 cut(s) 25
Bsp68I TCGCGA 1 cut(s) 342
BspANI GGCC 1 cut(s) 497
BspFNI CGCG 4 cut(s) 342, 383, 473, 687
BspLI GGNNCC 1 cut(s) 215
BspPI GGATC 1 cut(s) 340
BspQI GCTCTTC 1 cut(s) 609
BsrBI CCGCTC 3 cut(s) 83, 549, 619
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 25, 271, 498
BssMI GATC 3 cut(s) 18, 345, 537
BssNI GRCGYC 3 cut(s) 71, 177, 426
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 5 cut(s) 33, 96, 103, 419, 449
Bst6I CTCTTC 6 cut(s) 46, 609, 629, 638, 653, 759
BstACI GRCGYC 3 cut(s) 71, 177, 426
BstBAI YACGTR 1 cut(s) 532
BstC8I GCNNGC 4 cut(s) 340, 547, 551, 797
BstDSI CCRYGG 2 cut(s) 25, 271
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 4 cut(s) 342, 383, 473, 687
BstHHI GCGC 3 cut(s) 385, 473, 526
BstKTI GATC 3 cut(s) 21, 348, 540
BstMAI GTCTC 3 cut(s) 51, 367, 631
BstMBI GATC 3 cut(s) 18, 345, 537
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 339, 542
BstNI CCWGG 1 cut(s) 499
BstPAI GACNNNNGTC 2 cut(s) 324, 447
BstSCI CCNGG 1 cut(s) 497
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 4 cut(s) 342, 383, 473, 687
BstV1I GCAGC 2 cut(s) 76, 468
BstV2I GAAGAC 1 cut(s) 256
BstX2I RGATCY 2 cut(s) 18, 345
BstYI RGATCY 2 cut(s) 18, 345
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 497
BtgI CCRYGG 2 cut(s) 25, 271
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 491
BtuMI TCGCGA 1 cut(s) 342
Cac8I GCNNGC 4 cut(s) 340, 547, 551, 797
CfoI GCGC 3 cut(s) 385, 473, 526
CseI GACGC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 409
CviAII CATG 3 cut(s) 26, 292, 728
CviQI GTAC 1 cut(s) 409
DpnI GATC 3 cut(s) 20, 347, 539
DpnII GATC 3 cut(s) 18, 345, 537
DraIII CACNNNGTG 1 cut(s) 532
DrdI GACNNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 182
Eam1104I CTCTTC 6 cut(s) 46, 609, 629, 638, 653, 759
EarI CTCTTC 6 cut(s) 46, 609, 629, 638, 653, 759
EciI GGCGGA 2 cut(s) 25, 206
Ecl136II GAGCTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 171
Eco57I CTGAAG 3 cut(s) 590, 662, 677
Eco72I CACGTG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 171
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 3 cut(s) 29, 295, 731
FaiI YATR 5 cut(s) 27, 293, 521, 669, 729
FaqI GGGAC 1 cut(s) 515
FatI CATG 3 cut(s) 25, 291, 727
FauI CCCGC 6 cut(s) 209, 272, 358, 506, 542, 680
Fnu4HI GCNGC 8 cut(s) 65, 81, 189, 237, 277, 300, 457, 653
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 8 cut(s) 65, 81, 189, 237, 277, 300, 457, 653
FspBI CTAG 1 cut(s) 704
GlaI GCGC 3 cut(s) 384, 472, 525
GluI GCNGC 8 cut(s) 65, 81, 189, 237, 277, 300, 457, 653
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 1 cut(s) 497
HapII CCGG 1 cut(s) 510
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 385, 473, 526
Hin1I GRCGYC 3 cut(s) 71, 177, 426
Hin1II CATG 3 cut(s) 29, 295, 731
Hin6I GCGC 3 cut(s) 383, 471, 524
HinP1I GCGC 3 cut(s) 383, 471, 524
HincII GTYRAC 4 cut(s) 99, 329, 370, 452
HindII GTYRAC 4 cut(s) 99, 329, 370, 452
HinfI GANTC 5 cut(s) 475, 592, 626, 751, 773
HpaII CCGG 1 cut(s) 510
HphI GGTGA 2 cut(s) 5, 84
Hpy166II GTNNAC 4 cut(s) 99, 329, 370, 452
Hpy188I TCNGA 4 cut(s) 175, 542, 581, 631
Hpy188III TCNNGA 3 cut(s) 166, 341, 563
Hpy8I GTNNAC 4 cut(s) 99, 329, 370, 452
HpyAV CCTTC 3 cut(s) 207, 298, 565
HpyCH4III ACNGT 5 cut(s) 33, 96, 103, 419, 449
HpyCH4IV ACGT 3 cut(s) 177, 426, 531
HpyCH4V TGCA 3 cut(s) 484, 789, 795
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 339, 542
HpySE526I ACGT 3 cut(s) 177, 426, 531
Hsp92I GRCGYC 3 cut(s) 71, 177, 426
Hsp92II CATG 3 cut(s) 29, 295, 731
HspAI GCGC 3 cut(s) 383, 471, 524
Kzo9I GATC 3 cut(s) 18, 345, 537
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 6 cut(s) 176, 255, 388, 512, 565, 700
Lsp1109I GCAGC 2 cut(s) 76, 468
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 704
MaeII ACGT 3 cut(s) 177, 426, 531
MaeIII GTNAC 1 cut(s) 808
MalI GATC 3 cut(s) 20, 347, 539
MbiI CCGCTC 3 cut(s) 83, 549, 619
MboI GATC 3 cut(s) 18, 345, 537
MboII GAAGA 9 cut(s) 33, 256, 259, 626, 646, 655, 670, 691, 746
MfeI CAATTG 1 cut(s) 790
MflI RGATCY 2 cut(s) 18, 345
MhlI GDGCHC 2 cut(s) 173, 555
MluCI AATT 2 cut(s) 557, 790
MlyI GAGTC 2 cut(s) 745, 782
MmeI TCCRAC 3 cut(s) 198, 559, 725
MseI TTAA 4 cut(s) 114, 143, 767, 803
MspA1I CMGCKG 2 cut(s) 191, 655
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 499
MunI CAATTG 1 cut(s) 790
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 4 cut(s) 342, 383, 473, 687
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 339, 542
NcoI CCATGG 1 cut(s) 25
NdeII GATC 3 cut(s) 18, 345, 537
NlaIII CATG 3 cut(s) 29, 295, 731
NlaIV GGNNCC 1 cut(s) 215
NruI TCGCGA 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 609
PcsI WCGNNNNNNNCGW 3 cut(s) 363, 420, 423
PfeI GAWTC 3 cut(s) 475, 592, 626
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 8 cut(s) 66, 82, 190, 238, 278, 301, 458, 654
PleI GAGTC 2 cut(s) 745, 781
PmaCI CACGTG 1 cut(s) 532
PmlI CACGTG 1 cut(s) 532
PpsI GAGTC 2 cut(s) 745, 781
Ppu21I YACGTR 1 cut(s) 532
PshAI GACNNNNGTC 2 cut(s) 324, 447
Psp124BI GAGCTC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 497
PspCI CACGTG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 215
PsuI RGATCY 2 cut(s) 18, 345
PsyI GACNNNGTC 1 cut(s) 179
RruI TCGCGA 1 cut(s) 342
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SacI GAGCTC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 4 cut(s) 114, 143, 767, 803
SatI GCNGC 8 cut(s) 65, 81, 189, 237, 277, 300, 457, 653
Sau3AI GATC 3 cut(s) 18, 345, 537
SchI GAGTC 2 cut(s) 745, 782
ScrFI CCNGG 1 cut(s) 499
SduI GDGCHC 2 cut(s) 173, 555
SfaNI GCATC 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 2 cut(s) 557, 790
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 497
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 5 cut(s) 33, 96, 103, 419, 449
TaiI ACGT 3 cut(s) 180, 429, 534
TaqI TCGA 3 cut(s) 423, 441, 465
TasI AATT 2 cut(s) 557, 790
TauI GCSGC 6 cut(s) 83, 191, 239, 279, 302, 655
TfiI GAWTC 3 cut(s) 475, 592, 626
Tru1I TTAA 4 cut(s) 114, 143, 767, 803
Tru9I TTAA 4 cut(s) 114, 143, 767, 803
TscAI CASTG 1 cut(s) 491
TseI GCWGC 2 cut(s) 64, 456
TspDTI ATGAA 3 cut(s) 280, 427, 716
TspGWI ACGGA 4 cut(s) 355, 384, 390, 638
TspRI CASTG 1 cut(s) 491
Tth111I GACNNNGTC 1 cut(s) 179
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XspI CTAG 1 cut(s) 704
ZraI GACGTC 2 cut(s) 178, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.