Rh5DG534300

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000184
Physical Location & Seq
Reverse (-)
82250178 .. 82251392
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG534300.1

Sequence Viewer

Length: 501 bp
ATGGGCCAGGTCCTCTTGAGCTTCTTCCTGTCAGCCTGTGACAGTCTCTGGTGCGACCGCGCTTCCACTCCCTTCTGCCAACGCCTCGCTCGCGAGCTCCCCTCCGTCGTTGTCTCCGTCAACTACCGCCTCGCGCTGGAGCATCGATATCCGTGCCAGTACGACGACGGTTTCGACGTCCTCAAGTTCATCGACGACAGTCAAAGCAACTTGTTCGAGGGCGTGAATCTCGGGCAGTGTTTTCTGGCCGGGGACAGCGCCGGCGGGAACATAGCGCACCACGTGGCGATCAGAGCGAGCGGGCACGAGTTTCGAGATCTGAAGGTGGTCGGGATCTTGGCGATTCAGCCGTTTTTCAGAGGAGAGGAGTGGACGGAATCGGAGACGAGACTAAAGAGTGTGCCGCTGGTGAACGTAGAGCGGACGGACTGGGTGTGGAGGGCGTTTTTTGCCGGAGGGGTCCGCGCGGTGAGGGGTAACAGAGGCCTAAGAATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

166

Amino Acids

18.7

Weight (kDa)

6.09

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 23 - 151 8.3e-31 alpha/beta hydrolase fold
BD-FAE PF20434 36 - 104 2.8e-06 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccBSI CCGCTC 2 cut(s) 300, 421
AccII CGCG 5 cut(s) 60, 93, 134, 465, 467
AciI CCGC 8 cut(s) 58, 127, 264, 300, 404, 421, 463, 467
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 341
AcvI CACGTG 1 cut(s) 283
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 283
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 136, 493
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 2 cut(s) 21, 97
AluI AGCT 2 cut(s) 21, 97
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 4 cut(s) 50, 118, 377, 382
AlwI GGATC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 3 cut(s) 4, 246, 484
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspLEI GCGC 5 cut(s) 62, 136, 260, 277, 467
AspS9I GGNCC 3 cut(s) 4, 10, 460
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 421, 481
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 2 cut(s) 10, 460
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 305
BbrPI CACGTG 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 334
BcgI CGANNNNNNTGC 6 cut(s) 135, 169, 196, 230, 293, 327
BciT130I CCWGG 1 cut(s) 8
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 4 cut(s) 50, 118, 377, 382
BfoI RGCGCY 1 cut(s) 261
BglII AGATCT 1 cut(s) 316
BisI GCNGC 1 cut(s) 404
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 2 cut(s) 8, 250
Bme18I GGWCC 2 cut(s) 10, 460
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 3 cut(s) 4, 10, 460
BmiI GGNNCC 1 cut(s) 461
BmrFI CCNGG 2 cut(s) 8, 250
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 439
BoxI GACNNNNGTC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 37, 167
BpuMI CCSGG 1 cut(s) 250
Bsa29I ATCGAT 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 283
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 493
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 157, 434
BseBI CCWGG 1 cut(s) 8
BseCI ATCGAT 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 136, 493
BseNI ACTGG 2 cut(s) 157, 434
BseRI GAGGAG 2 cut(s) 375, 380
BseSI GKGCMC 1 cut(s) 306
Bsh1236I CGCG 5 cut(s) 60, 93, 134, 465, 467
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 3 cut(s) 6, 248, 486
BshVI ATCGAT 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 3 cut(s) 249, 261, 453
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 136, 493
BsmAI GTCTC 4 cut(s) 50, 118, 377, 382
BsmBI CGTCTC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 3 cut(s) 6, 248, 486
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 2 cut(s) 99, 306
Bsp143I GATC 3 cut(s) 288, 316, 333
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 8 cut(s) 58, 127, 264, 300, 404, 421, 463, 467
BspANI GGCC 3 cut(s) 6, 248, 486
BspDI ATCGAT 1 cut(s) 145
BspFNI CGCG 5 cut(s) 60, 93, 134, 465, 467
BspLI GGNNCC 1 cut(s) 461
BspPI GGATC 1 cut(s) 341
BsrBI CCGCTC 2 cut(s) 300, 421
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 157, 434
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 3 cut(s) 288, 316, 333
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 3 cut(s) 44, 170, 200
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 283
BstC8I GCNNGC 5 cut(s) 91, 95, 262, 298, 302
BstDEI CTNAG 1 cut(s) 488
BstFNI CGCG 5 cut(s) 60, 93, 134, 465, 467
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 5 cut(s) 62, 136, 260, 277, 467
BstKTI GATC 3 cut(s) 291, 319, 336
BstMAI GTCTC 4 cut(s) 50, 118, 377, 382
BstMBI GATC 3 cut(s) 288, 316, 333
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 90, 293, 449
BstNI CCWGG 1 cut(s) 8
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 2 cut(s) 6, 248
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 5 cut(s) 60, 93, 134, 465, 467
BstX2I RGATCY 2 cut(s) 316, 333
BstYI RGATCY 2 cut(s) 316, 333
Bsu15I ATCGAT 1 cut(s) 145
BsuRI GGCC 3 cut(s) 6, 248, 486
BsuTUI ATCGAT 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 1 cut(s) 242
BtuMI TCGCGA 1 cut(s) 93
Cac8I GCNNGC 5 cut(s) 91, 95, 262, 298, 302
CaiI CAGNNNCTG 1 cut(s) 48
CfoI GCGC 5 cut(s) 62, 136, 260, 277, 467
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 4, 10, 460
ClaI ATCGAT 1 cut(s) 145
Csp6I GTAC 1 cut(s) 160
CviJI RGCY 7 cut(s) 6, 21, 35, 97, 248, 349, 486
CviKI_1 RGCY 7 cut(s) 6, 21, 35, 97, 248, 349, 486
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 488
DpnI GATC 3 cut(s) 290, 318, 335
DpnII GATC 3 cut(s) 288, 316, 333
DraIII CACNNNGTG 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 97
Eco147I AGGCCT 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 10, 460
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 341
Eco72I CACGTG 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 6
EcoRV GATATC 1 cut(s) 149
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 377
FaiI YATR 1 cut(s) 272
FaqI GGGAC 1 cut(s) 266
FauI CCCGC 2 cut(s) 257, 293
Fnu4HI GCNGC 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 404
GlaI GCGC 5 cut(s) 61, 135, 259, 276, 466
GluI GCNGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 3 cut(s) 6, 248, 486
HapII CCGG 3 cut(s) 249, 261, 453
HhaI GCGC 5 cut(s) 62, 136, 260, 277, 467
Hin1I GRCGYC 1 cut(s) 177
Hin6I GCGC 5 cut(s) 60, 134, 258, 275, 465
HinP1I GCGC 5 cut(s) 60, 134, 258, 275, 465
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 3 cut(s) 226, 343, 377
HpaII CCGG 3 cut(s) 249, 261, 453
HphI GGTGA 2 cut(s) 421, 481
Hpy166II GTNNAC 3 cut(s) 121, 372, 412
Hpy188I TCNGA 4 cut(s) 293, 321, 359, 382
Hpy188III TCNNGA 4 cut(s) 16, 92, 314, 331
Hpy8I GTNNAC 3 cut(s) 121, 372, 412
Hpy99I CGWCG 5 cut(s) 110, 167, 170, 179, 197
HpyAV CCTTC 2 cut(s) 82, 316
HpyCH4III ACNGT 3 cut(s) 44, 170, 200
HpyCH4IV ACGT 3 cut(s) 177, 282, 414
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 293, 449
HpyF3I CTNAG 1 cut(s) 488
HpySE526I ACGT 3 cut(s) 177, 282, 414
Hsp92I GRCGYC 1 cut(s) 177
HspAI GCGC 5 cut(s) 60, 134, 258, 275, 465
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Kzo9I GATC 3 cut(s) 288, 316, 333
LmnI GCTCC 2 cut(s) 102, 139
LweI GCATC 1 cut(s) 151
MaeII ACGT 3 cut(s) 177, 282, 414
MaeIII GTNAC 2 cut(s) 38, 476
MalI GATC 3 cut(s) 290, 318, 335
MbiI CCGCTC 2 cut(s) 300, 421
MboI GATC 3 cut(s) 288, 316, 333
MboII GAAGA 1 cut(s) 16
MflI RGATCY 2 cut(s) 316, 333
MhlI GDGCHC 2 cut(s) 99, 306
MreI CGCCGGCG 1 cut(s) 260
MroNI GCCGGC 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 3 cut(s) 249, 261, 453
MspR9I CCNGG 2 cut(s) 8, 250
MvaI CCWGG 1 cut(s) 8
MvnI CGCG 5 cut(s) 60, 93, 134, 465, 467
MwoI GCNNNNNNNGC 3 cut(s) 90, 293, 449
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 250
NdeII GATC 3 cut(s) 288, 316, 333
NgoMIV GCCGGC 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 38
NruI TCGCGA 1 cut(s) 93
PceI AGGCCT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 171, 174
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 3 cut(s) 226, 343, 377
PkrI GCNGC 1 cut(s) 405
PmaCI CACGTG 1 cut(s) 283
PmlI CACGTG 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 10
PshAI GACNNNNGTC 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 6
PspCI CACGTG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 461
PspPI GGNCC 3 cut(s) 4, 10, 460
PspPPI RGGWCCY 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 48
PsuI RGATCY 2 cut(s) 316, 333
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SacI GAGCTC 1 cut(s) 99
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 3 cut(s) 288, 316, 333
Sau96I GGNCC 3 cut(s) 4, 10, 460
ScrFI CCNGG 2 cut(s) 8, 250
SduI GDGCHC 2 cut(s) 99, 306
SetI ASST 7 cut(s) 12, 23, 99, 180, 285, 327, 417
SfaNI GCATC 1 cut(s) 151
SgrAI CRCCGGYG 1 cut(s) 260
SinI GGWCC 2 cut(s) 10, 460
SmlI CTYRAG 2 cut(s) 16, 182
SmoI CTYRAG 2 cut(s) 16, 182
SseBI AGGCCT 1 cut(s) 486
SsiI CCGC 8 cut(s) 58, 127, 264, 300, 404, 421, 463, 467
SstI GAGCTC 1 cut(s) 99
StuI AGGCCT 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 6, 248
TaaI ACNGT 3 cut(s) 44, 170, 200
TaiI ACGT 3 cut(s) 180, 285, 417
TaqI TCGA 5 cut(s) 145, 174, 192, 216, 313
TauI GCSGC 1 cut(s) 406
TfiI GAWTC 3 cut(s) 226, 343, 377
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 5 cut(s) 94, 106, 141, 389, 440
TspRI CASTG 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 10, 460
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.